pycom03g04060

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007364
Physical Location & Seq
Forward (+)
3202089 .. 3205323
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1431 bp
ATGCGGCGGATCATGACCGTCCCCTTCTTCACCAACAAGGTCGTCCAGCTGTACCGCCACGGATTGGAATCGGAGGCGGCGGCTGTCGTCGAGGACGTGAAAAAGCAGCCTGAGGCGGCGACCAGTGGGATGGTGTTGCGGAGGCGGCTGCAGCTGATGATGTACAACAACGTATACAGAATCATGTTAGATCGGCGATTCGAGAGCAAGGAGGATCTCCTGTTCGAGAAGCTGAAGGGATCGAACGGGGAGAAGAGCGGATTGGCGCAGAGCTTCGATTACAACTACGGCGATTTTATCCCGATATTAAGGCCGTTTTTGAGAGGGTATTTGAAGATGTGCAAGGAAGTGAAGGAGAAGAGGATGCAGCTGTTTAAGGACTATTTCATTGACGAGAGGAAGAAAATTTGGAGCACACAGGCTACAACAAATGAAGGGTTGAAGTGCGCCATTGACCACCTCCTCGACGCGCAGCACAAGGGAGAAATCAACGACGACAATATTTACTACATCCTTGACAACATCAATGCCGCTGCTATTGACACCACTTTGTGGTCGATCGAGTGGGGAATTGTCGAGCTAGTGAACCACCCTGAAATCCAGAAGAAGTTGAGTGAAGAACTTGATGCAGTCCTCGGCCGCGGAGTTCAGATCACAGAGCCCGACGTTCAGAAACTTCCCTACCTGCAAGCCGCGATCAAGGAGACTCTCCGACTTCACATGCCAACCCCACTCCTTGTTCCCCACATGAACCTCCTGGACGCCAAGCTTGGCGGGTTTGACATCCCGGCGGAAAGCAAGATTTTGGTGAATGCTGGGGGGTTGGCAAACAACCCTGCCTTGTGGAAGAAGCCAGAGGAGTTTAGGCCCGAGAGGTTTTTGGAGGAGTCGTCGAAGGTGGAGGCTAATAGGAACGACTTCAGGTATCTCCCGTTTGGTGTTGGGAGAAGAAGTTGTCCGGGAATTATCCTAGCCCTTTCAATTTTGGGCATTACAATTGGACGTTTAGTCCAGAACTTGAAGCTTCTTCCTCCGCCGGGACAGTCCAAGGTCGACACCTCGGAGAAATGTGGGCAGTTCACCCTGCAGATCCTTAAGCATTCCACCATTGTGATGAAGCCGAGGGGGCCTCTAACAAAAGAGCTTCAAAAAAAGCACTTGTTGACAGAAGTGCTTTCCATAGAAGCACTTTTAAACAAGAACCCGGTTCAGGGGCTAAAAAGCAGAAAAAATTCGATCTCGAAAACAAGAAAAAAAAAAGGAGGAAATGAGATAGAAAAGGTAATTTGGGTCGTAACCAGGCCCTCAAATCAGAGAAATTCAGAGAGGGCAGACATGCGGACCCTTTGCAATTCAATTTCCGTGCACATAACCATGCTTTCTTCATTCTTTTGTATCCAAAGCATCGGCTTATTGTGCACGGATCCGTAA

Protein Analysis

477

Amino Acids

54.17

Weight (kDa)

9.33

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 367 3.1e-85 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 693
AccB7I CCANNNNNTGG 2 cut(s) 64, 552
AccBSI CCGCTC 1 cut(s) 258
AccI GTMKAC 2 cut(s) 174, 1053
AccII CGCG 3 cut(s) 470, 642, 695
AclWI GGATC 5 cut(s) 17, 222, 247, 1084, 1418
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 3 cut(s) 405, 1231, 1318
AcuI CTGAAG 2 cut(s) 254, 904
AcyI GRCGYC 1 cut(s) 762
AdeI CACNNNGTG 1 cut(s) 552
AfaI GTAC 2 cut(s) 53, 164
AfiI CCNNNNNNNGG 5 cut(s) 64, 552, 1037, 1210, 1211
AflII CTTAAG 1 cut(s) 1094
AgsI TTSAA 6 cut(s) 334, 442, 981, 1021, 1148, 1356
AhdI GACNNNNNGTC 1 cut(s) 1007
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 2 cut(s) 756, 1298
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AleI CACNNNNGTG 1 cut(s) 1109
AloI GAACNNNNNNTCC 2 cut(s) 206, 238
AluBI AGCT 9 cut(s) 49, 154, 232, 273, 370, 580, 769, 1024, 1144
AluI AGCT 9 cut(s) 49, 154, 232, 273, 370, 580, 769, 1024, 1144
Alw21I GWGCWC 3 cut(s) 416, 1368, 1421
Alw26I GTCTC 1 cut(s) 698
Alw44I GTGCAC 2 cut(s) 1364, 1417
AlwI GGATC 5 cut(s) 17, 222, 247, 1084, 1418
Ama87I CYCGRG 1 cut(s) 869
AoxI GGCC 5 cut(s) 311, 637, 866, 1127, 1301
ApaLI GTGCAC 2 cut(s) 1364, 1417
ApeKI GCWGC 6 cut(s) 106, 148, 151, 367, 472, 533
ApoI RAATTY 3 cut(s) 405, 1231, 1318
Asp700I GAANNNNTTC 2 cut(s) 917, 1231
AspLEI GCGC 3 cut(s) 268, 449, 472
AspS9I GGNCC 4 cut(s) 867, 1127, 1302, 1341
AsuC2I CCSGG 4 cut(s) 788, 960, 1038, 1205
AsuHPI GGTGA 3 cut(s) 22, 820, 1072
AvaI CYCGRG 1 cut(s) 869
AvaII GGWCC 1 cut(s) 1341
AxyI CCTNAGG 1 cut(s) 111
BaeGI GKGCMC 2 cut(s) 1368, 1421
BamHI GGATCC 1 cut(s) 1423
BanII GRGCYC 1 cut(s) 663
Bbv12I GWGCWC 3 cut(s) 416, 1368, 1421
BbvI GCAGC 6 cut(s) 118, 135, 163, 379, 484, 520
BccI CCATC 1 cut(s) 124
BceAI ACGGC 2 cut(s) 298, 304
BciT130I CCWGG 2 cut(s) 758, 1300
BciVI GTATCC 1 cut(s) 1406
BcnI CCSGG 4 cut(s) 788, 960, 1038, 1205
BcoDI GTCTC 1 cut(s) 698
BfaI CTAG 2 cut(s) 581, 971
BfmI CTRYAG 2 cut(s) 149, 1085
BfrI CTTAAG 1 cut(s) 1094
BfuAI ACCTGC 1 cut(s) 693
BfuI GTATCC 1 cut(s) 1406
BglI GCCNNNNNGGC 1 cut(s) 1126
Bme1390I CCNGG 6 cut(s) 758, 788, 960, 1038, 1205, 1300
Bme18I GGWCC 1 cut(s) 1341
BmeRI GACNNNNNGTC 1 cut(s) 1007
BmeT110I CYCGRG 1 cut(s) 869
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 4 cut(s) 867, 1127, 1302, 1341
BmiI GGNNCC 3 cut(s) 1128, 1343, 1425
BmrFI CCNGG 6 cut(s) 758, 788, 960, 1038, 1205, 1300
BmsI GCATC 3 cut(s) 354, 616, 1413
BplI GAGNNNNNCTC 2 cut(s) 1114, 1146
BpuMI CCSGG 4 cut(s) 788, 960, 1038, 1205
BsaBI GATNNNNATC 1 cut(s) 67
BsaHI GRCGYC 1 cut(s) 762
BsaJI CCNNGG 6 cut(s) 58, 634, 640, 1047, 1059, 1121
BsaXI ACNNNNNCTCC 4 cut(s) 875, 905, 937, 967
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 552, 1037, 1210, 1211
Bse1I ACTGG 1 cut(s) 123
Bse21I CCTNAGG 1 cut(s) 111
Bse8I GATNNNNATC 1 cut(s) 67
BseBI CCWGG 2 cut(s) 758, 1300
BseDI CCNNGG 6 cut(s) 58, 634, 640, 1047, 1059, 1121
BseGI GGATG 4 cut(s) 135, 369, 510, 783
BseJI GATNNNNATC 1 cut(s) 67
BseLI CCNNNNNNNGG 5 cut(s) 64, 552, 1037, 1210, 1211
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 123
BseRI GAGGAG 3 cut(s) 452, 872, 899
BseSI GKGCMC 2 cut(s) 1368, 1421
BseX3I CGGCCG 1 cut(s) 637
BseXI GCAGC 6 cut(s) 118, 135, 163, 379, 484, 520
BseYI CCCAGC 1 cut(s) 815
Bsh1236I CGCG 3 cut(s) 470, 642, 695
Bsh1285I CGRYCG 2 cut(s) 561, 640
BshFI GGCC 5 cut(s) 313, 639, 868, 1129, 1303
BsiEI CGRYCG 2 cut(s) 561, 640
BsiHKAI GWGCWC 3 cut(s) 416, 1368, 1421
BsiHKCI CYCGRG 1 cut(s) 869
BsiSI CCGG 4 cut(s) 788, 959, 1037, 1205
BslFI GGGAC 2 cut(s) 5, 1053
BslI CCNNNNNNNGG 5 cut(s) 64, 552, 1037, 1210, 1211
BsmAI GTCTC 1 cut(s) 698
BsmFI GGGAC 2 cut(s) 5, 1053
BsmI GAATGC 2 cut(s) 817, 1099
BsnI GGCC 5 cut(s) 313, 639, 868, 1129, 1303
BsoBI CYCGRG 1 cut(s) 869
Bsp1286I GDGCHC 4 cut(s) 416, 663, 1368, 1421
Bsp1407I TGTACA 1 cut(s) 162
BspANI GGCC 5 cut(s) 313, 639, 868, 1129, 1303
BspCNI CTCAG 1 cut(s) 103
BspFNI CGCG 3 cut(s) 470, 642, 695
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 3 cut(s) 1128, 1343, 1425
BspMAI CTGCAG 2 cut(s) 153, 1089
BspMI ACCTGC 1 cut(s) 693
BspPI GGATC 5 cut(s) 17, 222, 247, 1084, 1418
BspQI GCTCTTC 1 cut(s) 248
BspTI CTTAAG 1 cut(s) 1094
BsrBI CCGCTC 1 cut(s) 258
BsrGI TGTACA 1 cut(s) 162
BsrI ACTGG 1 cut(s) 123
BssECI CCNNGG 6 cut(s) 58, 634, 640, 1047, 1059, 1121
BssNAI GTATAC 1 cut(s) 175
BssNI GRCGYC 1 cut(s) 762
BssT1I CCWWGG 1 cut(s) 1047
Bst1107I GTATAC 1 cut(s) 175
Bst2UI CCWGG 2 cut(s) 758, 1300
Bst4CI ACNGT 2 cut(s) 19, 1044
Bst6I CTCTTC 2 cut(s) 248, 353
BstACI GRCGYC 1 cut(s) 762
BstAFI CTTAAG 1 cut(s) 1094
BstAUI TGTACA 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 1 cut(s) 111
BstDSI CCRYGG 2 cut(s) 58, 640
BstF5I GGATG 4 cut(s) 135, 369, 510, 783
BstFNI CGCG 3 cut(s) 470, 642, 695
BstHHI GCGC 3 cut(s) 268, 449, 472
BstMAI GTCTC 1 cut(s) 698
BstMCI CGRYCG 2 cut(s) 561, 640
BstMWI GCNNNNNNNGC 4 cut(s) 145, 151, 1126, 1416
BstNI CCWGG 2 cut(s) 758, 1300
BstNSI RCATGY 2 cut(s) 724, 1339
BstSCI CCNGG 6 cut(s) 756, 786, 958, 1036, 1203, 1298
BstSFI CTRYAG 2 cut(s) 149, 1085
BstSLI GKGCMC 2 cut(s) 1368, 1421
BstUI CGCG 3 cut(s) 470, 642, 695
BstV1I GCAGC 6 cut(s) 118, 135, 163, 379, 484, 520
BstX2I RGATCY 3 cut(s) 214, 1089, 1423
BstXI CCANNNNNNTGG 1 cut(s) 130
BstYI RGATCY 3 cut(s) 214, 1089, 1423
BstZ17I GTATAC 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 637
Bsu36I CCTNAGG 1 cut(s) 111
BsuI GTATCC 1 cut(s) 1406
BsuRI GGCC 5 cut(s) 313, 639, 868, 1129, 1303
BtgI CCRYGG 2 cut(s) 58, 640
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 4 cut(s) 135, 369, 510, 783
BtsIMutI CAGTG 1 cut(s) 130
BveI ACCTGC 1 cut(s) 693
Cac8I GCNNGC 1 cut(s) 690
CciI TCATGA 1 cut(s) 12
CfoI GCGC 3 cut(s) 268, 449, 472
Cfr13I GGNCC 4 cut(s) 867, 1127, 1302, 1341
Cfr42I CCGCGG 1 cut(s) 643
CseI GACGC 2 cut(s) 476, 770
Csp6I GTAC 2 cut(s) 52, 163
CviAII CATG 6 cut(s) 13, 184, 721, 748, 1336, 1375
CviQI GTAC 2 cut(s) 52, 163
DdeI CTNAG 1 cut(s) 111
DraI TTTAAA 1 cut(s) 1194
DraIII CACNNNGTG 1 cut(s) 552
DriI GACNNNNNGTC 1 cut(s) 1007
EaeI YGGCCR 1 cut(s) 637
EagI CGGCCG 1 cut(s) 637
Eam1104I CTCTTC 2 cut(s) 248, 353
Eam1105I GACNNNNNGTC 1 cut(s) 1007
EarI CTCTTC 2 cut(s) 248, 353
EciI GGCGGA 3 cut(s) 22, 806, 1023
EclXI CGGCCG 1 cut(s) 637
Eco130I CCWWGG 1 cut(s) 1047
Eco24I GRGCYC 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 1341
Eco52I CGGCCG 1 cut(s) 637
Eco57I CTGAAG 2 cut(s) 254, 904
Eco81I CCTNAGG 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 869
EcoO109I RGGNCCY 2 cut(s) 1127, 1302
EcoRII CCWGG 2 cut(s) 756, 1298
EcoT14I CCWWGG 1 cut(s) 1047
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 1047
FaeI CATG 6 cut(s) 16, 187, 724, 751, 1339, 1378
FaiI YATR 9 cut(s) 14, 175, 185, 722, 749, 1181, 1337, 1370, 1376
FaqI GGGAC 2 cut(s) 5, 1053
FatI CATG 6 cut(s) 12, 183, 720, 747, 1335, 1374
FauI CCCGC 1 cut(s) 767
FblI GTMKAC 2 cut(s) 174, 1053
FokI GGATG 4 cut(s) 142, 376, 497, 770
FriOI GRGCYC 1 cut(s) 663
FspBI CTAG 2 cut(s) 581, 971
GlaI GCGC 3 cut(s) 267, 448, 471
GsaI CCCAGC 1 cut(s) 819
HaeIII GGCC 5 cut(s) 313, 639, 868, 1129, 1303
HapII CCGG 4 cut(s) 788, 959, 1037, 1205
HgaI GACGC 2 cut(s) 476, 770
HhaI GCGC 3 cut(s) 268, 449, 472
Hin1I GRCGYC 1 cut(s) 762
Hin1II CATG 6 cut(s) 16, 187, 724, 751, 1339, 1378
Hin6I GCGC 3 cut(s) 266, 447, 470
HinP1I GCGC 3 cut(s) 266, 447, 470
HincII GTYRAC 2 cut(s) 1054, 1164
HindII GTYRAC 2 cut(s) 1054, 1164
HindIII AAGCTT 2 cut(s) 767, 1022
HinfI GANTC 5 cut(s) 68, 180, 198, 706, 887
HpaII CCGG 4 cut(s) 788, 959, 1037, 1205
HphI GGTGA 3 cut(s) 22, 820, 1072
Hpy166II GTNNAC 7 cut(s) 175, 586, 1054, 1080, 1164, 1366, 1419
Hpy188I TCNGA 7 cut(s) 73, 651, 672, 713, 1063, 1314, 1324
Hpy188III TCNNGA 7 cut(s) 13, 202, 226, 301, 601, 1012, 1240
Hpy8I GTNNAC 7 cut(s) 175, 586, 1054, 1080, 1164, 1366, 1419
Hpy99I CGWCG 5 cut(s) 92, 470, 497, 668, 895
HpyAV CCTTC 5 cut(s) 34, 229, 346, 428, 889
HpyCH4III ACNGT 2 cut(s) 19, 1044
HpyCH4IV ACGT 4 cut(s) 96, 171, 666, 1003
HpyCH4V TGCA 9 cut(s) 151, 342, 367, 629, 688, 1087, 1350, 1366, 1419
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 151, 1126, 1416
HpyF3I CTNAG 1 cut(s) 111
HpySE526I ACGT 4 cut(s) 96, 171, 666, 1003
Hsp92I GRCGYC 1 cut(s) 762
Hsp92II CATG 6 cut(s) 16, 187, 724, 751, 1339, 1378
HspAI GCGC 3 cut(s) 266, 447, 470
KspI CCGCGG 1 cut(s) 643
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 1 cut(s) 411
Lsp1109I GCAGC 6 cut(s) 118, 135, 163, 379, 484, 520
LweI GCATC 3 cut(s) 354, 616, 1413
MaeI CTAG 2 cut(s) 581, 971
MaeII ACGT 4 cut(s) 96, 171, 666, 1003
MaeIII GTNAC 1 cut(s) 1294
MbiI CCGCTC 1 cut(s) 258
MfeI CAATTG 1 cut(s) 996
MflI RGATCY 3 cut(s) 214, 1089, 1423
MhlI GDGCHC 4 cut(s) 416, 663, 1368, 1421
MlyI GAGTC 2 cut(s) 700, 896
MmeI TCCRAC 1 cut(s) 736
MroXI GAANNNNTTC 2 cut(s) 917, 1231
MseI TTAA 4 cut(s) 308, 375, 1095, 1193
MslI CAYNNNNRTG 3 cut(s) 1109, 1112, 1373
MspA1I CMGCKG 5 cut(s) 49, 154, 370, 533, 642
MspCI CTTAAG 1 cut(s) 1094
MspI CCGG 4 cut(s) 788, 959, 1037, 1205
MspR9I CCNGG 6 cut(s) 758, 788, 960, 1038, 1205, 1300
MunI CAATTG 1 cut(s) 996
Mva1269I GAATGC 2 cut(s) 817, 1099
MvaI CCWGG 2 cut(s) 758, 1300
MvnI CGCG 3 cut(s) 470, 642, 695
MwoI GCNNNNNNNGC 4 cut(s) 145, 151, 1126, 1416
NciI CCSGG 4 cut(s) 788, 960, 1038, 1205
NlaIII CATG 6 cut(s) 16, 187, 724, 751, 1339, 1378
NlaIV GGNNCC 3 cut(s) 1128, 1343, 1425
NmeAIII GCCGAG 2 cut(s) 615, 1146
NspI RCATGY 2 cut(s) 724, 1339
OliI CACNNNNGTG 1 cut(s) 1109
PagI TCATGA 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 248
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 2 cut(s) 817, 1099
PdmI GAANNNNTTC 2 cut(s) 917, 1231
PfeI GAWTC 3 cut(s) 68, 180, 198
PflMI CCANNNNNTGG 2 cut(s) 64, 552
PfoI TCCNGGA 2 cut(s) 756, 958
Ple19I CGATCG 1 cut(s) 561
PleI GAGTC 2 cut(s) 700, 895
PpsI GAGTC 2 cut(s) 700, 895
Psp6I CCWGG 2 cut(s) 756, 1298
PspFI CCCAGC 1 cut(s) 815
PspGI CCWGG 2 cut(s) 756, 1298
PspN4I GGNNCC 3 cut(s) 1128, 1343, 1425
PspPI GGNCC 4 cut(s) 867, 1127, 1302, 1341
PstI CTGCAG 2 cut(s) 153, 1089
PsuI RGATCY 3 cut(s) 214, 1089, 1423
PvuI CGATCG 1 cut(s) 561
PvuII CAGCTG 3 cut(s) 49, 154, 370
RsaI GTAC 2 cut(s) 53, 164
RsaNI GTAC 2 cut(s) 52, 163
RseI CAYNNNNRTG 3 cut(s) 1109, 1112, 1373
SacII CCGCGG 1 cut(s) 643
SalI GTCGAC 1 cut(s) 1052
SapI GCTCTTC 1 cut(s) 248
SaqAI TTAA 4 cut(s) 308, 375, 1095, 1193
Sau96I GGNCC 4 cut(s) 867, 1127, 1302, 1341
SchI GAGTC 2 cut(s) 700, 896
ScrFI CCNGG 6 cut(s) 758, 788, 960, 1038, 1205, 1300
SduI GDGCHC 4 cut(s) 416, 663, 1368, 1421
SfaNI GCATC 3 cut(s) 354, 616, 1413
SfcI CTRYAG 2 cut(s) 149, 1085
Sfr303I CCGCGG 1 cut(s) 643
SgrBI CCGCGG 1 cut(s) 643
SinI GGWCC 1 cut(s) 1341
SmiMI CAYNNNNRTG 3 cut(s) 1109, 1112, 1373
SmlI CTYRAG 1 cut(s) 1094
SmoI CTYRAG 1 cut(s) 1094
SspI AATATT 1 cut(s) 502
SspMI CTAG 2 cut(s) 581, 971
StyD4I CCNGG 6 cut(s) 756, 786, 958, 1036, 1203, 1298
StyI CCWWGG 1 cut(s) 1047
TaaI ACNGT 2 cut(s) 19, 1044
TaiI ACGT 4 cut(s) 99, 174, 669, 1006
TatI WGTACW 1 cut(s) 162
TauI GCSGC 8 cut(s) 7, 80, 83, 119, 148, 533, 642, 695
TfiI GAWTC 3 cut(s) 68, 180, 198
Tru1I TTAA 4 cut(s) 308, 375, 1095, 1193
Tru9I TTAA 4 cut(s) 308, 375, 1095, 1193
TscAI CASTG 1 cut(s) 130
TseI GCWGC 6 cut(s) 106, 148, 151, 367, 472, 533
TspDTI ATGAA 5 cut(s) 376, 447, 764, 1130, 1374
TspGWI ACGGA 3 cut(s) 75, 1351, 1416
TspRI CASTG 1 cut(s) 130
Van91I CCANNNNNTGG 2 cut(s) 64, 552
Vha464I CTTAAG 1 cut(s) 1094
VneI GTGCAC 2 cut(s) 1364, 1417
VpaK11BI GGWCC 1 cut(s) 1341
XapI RAATTY 3 cut(s) 405, 1231, 1318
XceI RCATGY 2 cut(s) 724, 1339
XmiI GTMKAC 2 cut(s) 174, 1053
XmnI GAANNNNTTC 2 cut(s) 917, 1231
XspI CTAG 2 cut(s) 581, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.