pycom03g07980

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011426
Physical Location & Seq
Forward (+)
6379038 .. 6382928
3891 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1425 bp
ATGGGGGACGCATCGCGTAGAATCCCTCCAGAGGCCAATGTGGTTATGAATGGAAAGACCAAGTCGCAGAATCATATAGTTGCTTCTCGCCCCAAGGGCTTTCTGCAGGATGTAGTGTCGCTTCGAATTGGGAGTCGCGTGGTGAGCCAGGCAGCTTTGTTTCTGCTCAAAATGGCTGCATTGGAGACAGTAAGGCGGTTCTCAAGGGCCAAGTGCCCGTGGGCATGGCGTGGATTCCAGGCTTTGCAATTTCTGTGTTACCCTCCGTTCAAGTGGTTTCGAAGATTTGCGCCCTTCAAGGCTTTGGTCAATGGCATGCAGGTAGTTTCAAAGCCATTACTAGTGCTTTCCATTGCAACGGTGTTCTCTGATGAAGCAGAGTTCTTTAATAGAACCTCAGATGGCATTAACAATTCTGATGCATGTTCAGAAGTGCAGTCGGAAGTATTGTCTGAGGGTATTAACGATTCTACTTTGGATACAAGGGTTCCTGATCAATTTCCTCAAAATGTAGAACCTGAAATGTGGTTGGTAGAACTCCATGAAGAGCTTGAAAGACAAGGGATTAGCTTGCCAGAAAGAATTGATGAGGAGGAACTTCGTAGGTTTTACACTGCTGCAAATGGAGACTTTTCTCGCTTGCTATCATCAGTAAAGAAGACAATCCATTGGAGGGAGACTTACGGAATTCTTTCAATACAGGAGCTTGAGATGTGGTCAAATATGGTTTTCTGGCATGGATTTGATGTTAAGCACCGACCTTGCCTCATAGTGCGACTTGGGCTAGCTTGCACTAGCTTGCCATCTCATGATAGACCTCGCTTTGCTCAAGCTATCATTTCCCAAGTAGAGCATGGAGTCTATCATTTGCTCGATGCCACCAATGCTCAAATTACAGTTGTAGTAGATTGTGAAGGTCTATCTCCATTGAAAATTCCCATGCAAGTTATGAGGACTTGTTCTTCTCTTTTGCAAGATCACTTCCCCAACCGTCTTGGCTGTCTGTTTGTTATTCGGCTTCCTCCAATGCTTCGTGTTATTGCTCAAACCTTTATTCAAGTTTTGAAACCTTATACCCAAGAGAAGTTGAGGATTGAAGGTGAGAGTTACCGGAAGATTCTCTCTGAGTACCTTGAGGCACTTCCATTATATCTTGGTGGCAAATGCCAGTGCAAAATATGTACAGATATGTGGCACCCTCGCACAAATGAAGTTATCAAGACAGAGCTGAGAGAAGATATGCACGAGGGTCGGACTCCACTTCCAGCCCATCCAACGTATGAGAATGAGGTTGACATGGAAGATAACTGGGATCAGTTGGTGAGGACTGCCATATTTGGTCTCCTTATGGTATGGGTTTTCATAGCATTCCTTTCTTTATTCTTTGACCCCGAAAGCCGTCCCTTTCCCTTTTCTCCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

475

Amino Acids

54.48

Weight (kDa)

6.48

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 240 - 387 6.8e-26 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011426)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46450 AT3G46450 AT3G46450
fragaria_vesca FvH4_3g35600
malus_domestica MD03G1096600.v1.1 MD11G1142800.v1.1
prunus_persica Prupe.6G080900_v2.0.a1
pyrus_communis pycom03g07980 pycom11g09290
rosa_chinensis RchiOBHm_Chr5g0063781
rosa_laevigata RLG00000035613
rosa_multiflora Rmu_sc0005685.1_g000020 Rmu_ssc0000309.1_g000026
rosa_roxburghii Rroxscaffold_1G00017060
rosa_rugosa Rorug05G0359900
rosa_samantha Rh5AG418400 Rh5BG434000 Rh5CG457200 Rh5DG447300
rosa_wichuraiana Rw5G039300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 310
AccB1I GGYRCC 1 cut(s) 1194
AccII CGCG 2 cut(s) 16, 138
AciI CCGC 1 cut(s) 196
AclWI GGATC 1 cut(s) 1320
AcsI RAATTY 2 cut(s) 687, 933
AfaI GTAC 2 cut(s) 1130, 1183
AfiI CCNNNNNNNGG 2 cut(s) 31, 673
AgsI TTSAA 9 cut(s) 271, 298, 330, 554, 696, 931, 1058, 1066, 1097
AhlI ACTAGT 1 cut(s) 340
AjnI CCWGG 2 cut(s) 147, 237
AluBI AGCT 8 cut(s) 155, 550, 570, 706, 788, 798, 833, 1228
AluI AGCT 8 cut(s) 155, 550, 570, 706, 788, 798, 833, 1228
Alw26I GTCTC 4 cut(s) 179, 621, 671, 1346
AlwI GGATC 1 cut(s) 1320
AoxI GGCC 2 cut(s) 33, 207
ApeKI GCWGC 3 cut(s) 152, 176, 617
ApoI RAATTY 2 cut(s) 687, 933
ArsI GACNNNNNNTTYG 2 cut(s) 118, 150
Asp700I GAANNNNTTC 1 cut(s) 691
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 1 cut(s) 207
AsuHPI GGTGA 3 cut(s) 154, 1112, 1333
AsuII TTCGAA 2 cut(s) 124, 280
AsuNHI GCTAGC 1 cut(s) 784
BaeGI GKGCMC 1 cut(s) 218
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BanI GGYRCC 1 cut(s) 1194
BarI GAAGNNNNNNTAC 2 cut(s) 105, 137
BauI CACGAG 1 cut(s) 1244
BbsI GAAGAC 1 cut(s) 665
BbvI GCAGC 3 cut(s) 163, 164, 604
BccI CCATC 3 cut(s) 395, 811, 1278
BceAI ACGGC 1 cut(s) 1383
BcgI CGANNNNNNTGC 2 cut(s) 1232, 1266
BciT130I CCWGG 2 cut(s) 149, 239
BciVI GTATCC 1 cut(s) 472
BclI TGATCA 1 cut(s) 493
BcoDI GTCTC 4 cut(s) 179, 621, 671, 1346
BcuI ACTAGT 1 cut(s) 340
BfaI CTAG 3 cut(s) 341, 785, 795
BfmI CTRYAG 1 cut(s) 104
BfuAI ACCTGC 1 cut(s) 310
BfuI GTATCC 1 cut(s) 472
BglI GCCNNNNNGGC 1 cut(s) 96
BisI GCNGC 3 cut(s) 153, 177, 618
BlsI GCNGC 3 cut(s) 154, 178, 619
Bme1390I CCNGG 2 cut(s) 149, 239
BmgT120I GGNCC 1 cut(s) 207
BmiI GGNNCC 2 cut(s) 489, 1196
BmrFI CCNGG 2 cut(s) 149, 239
BmrI ACTGGG 1 cut(s) 1318
BmsI GCATC 3 cut(s) 20, 409, 865
BmtI GCTAGC 1 cut(s) 788
BmuI ACTGGG 1 cut(s) 1318
BpiI GAAGAC 1 cut(s) 665
BpmI CTGGAG 1 cut(s) 12
Bpu14I TTCGAA 2 cut(s) 124, 280
BpuEI CTTGAG 4 cut(s) 187, 728, 813, 1154
BsaI GGTCTC 1 cut(s) 1346
BsaJI CCNNGG 2 cut(s) 93, 218
BsaWI WCCGGW 1 cut(s) 1110
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 673
Bse1I ACTGG 2 cut(s) 1168, 1313
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 2 cut(s) 149, 239
BseDI CCNNGG 2 cut(s) 93, 218
BseGI GGATG 2 cut(s) 115, 1270
BseLI CCNNNNNNNGG 2 cut(s) 31, 673
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 4 cut(s) 411, 444, 1116, 1220
BseNI ACTGG 2 cut(s) 1168, 1313
BseRI GAGGAG 1 cut(s) 605
BseSI GKGCMC 1 cut(s) 218
BseXI GCAGC 3 cut(s) 163, 164, 604
BsgI GTGCAG 1 cut(s) 455
Bsh1236I CGCG 2 cut(s) 16, 138
BshFI GGCC 2 cut(s) 35, 209
BshNI GGYRCC 1 cut(s) 1194
BsiSI CCGG 1 cut(s) 1111
BslFI GGGAC 2 cut(s) 20, 1386
BslI CCNNNNNNNGG 2 cut(s) 31, 673
BsmAI GTCTC 4 cut(s) 179, 621, 671, 1346
BsmFI GGGAC 2 cut(s) 20, 1386
BsmI GAATGC 1 cut(s) 1367
BsnI GGCC 2 cut(s) 35, 209
Bso31I GGTCTC 1 cut(s) 1346
Bsp119I TTCGAA 2 cut(s) 124, 280
Bsp1286I GDGCHC 1 cut(s) 218
Bsp1407I TGTACA 1 cut(s) 1181
Bsp143I GATC 3 cut(s) 493, 976, 1312
BspACI CCGC 1 cut(s) 196
BspANI GGCC 2 cut(s) 35, 209
BspCNI CTCAG 4 cut(s) 410, 445, 1117, 1221
BspFNI CGCG 2 cut(s) 16, 138
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 2 cut(s) 489, 1196
BspMAI CTGCAG 1 cut(s) 108
BspMI ACCTGC 1 cut(s) 310
BspOI GCTAGC 1 cut(s) 788
BspPI GGATC 1 cut(s) 1320
BspQI GCTCTTC 1 cut(s) 540
BspT104I TTCGAA 2 cut(s) 124, 280
BspT107I GGYRCC 1 cut(s) 1194
BspTNI GGTCTC 1 cut(s) 1346
BsrDI GCAATG 1 cut(s) 351
BsrGI TGTACA 1 cut(s) 1181
BsrI ACTGG 2 cut(s) 1168, 1313
BssECI CCNNGG 2 cut(s) 93, 218
BssMI GATC 3 cut(s) 493, 976, 1312
BssSI CACGAG 1 cut(s) 1244
BssT1I CCWWGG 1 cut(s) 93
Bst2BI CACGAG 1 cut(s) 1244
Bst2UI CCWGG 2 cut(s) 149, 239
Bst4CI ACNGT 4 cut(s) 190, 361, 898, 992
Bst6I CTCTTC 1 cut(s) 540
BstAUI TGTACA 1 cut(s) 1181
BstBI TTCGAA 2 cut(s) 124, 280
BstC8I GCNNGC 6 cut(s) 317, 572, 641, 786, 790, 800
BstDEI CTNAG 5 cut(s) 397, 453, 1125, 1229, 1419
BstDSI CCRYGG 1 cut(s) 218
BstF5I GGATG 2 cut(s) 115, 1270
BstFNI CGCG 2 cut(s) 16, 138
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 3 cut(s) 496, 979, 1315
BstMAI GTCTC 4 cut(s) 179, 621, 671, 1346
BstMBI GATC 3 cut(s) 493, 976, 1312
BstMWI GCNNNNNNNGC 4 cut(s) 96, 144, 781, 884
BstNI CCWGG 2 cut(s) 149, 239
BstNSI RCATGY 2 cut(s) 319, 426
BstSCI CCNGG 2 cut(s) 147, 237
BstSFI CTRYAG 1 cut(s) 104
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 2 cut(s) 16, 138
BstV1I GCAGC 3 cut(s) 163, 164, 604
BstV2I GAAGAC 1 cut(s) 665
BsuI GTATCC 1 cut(s) 472
BsuRI GGCC 2 cut(s) 35, 209
BtgI CCRYGG 1 cut(s) 218
BtsCI GGATG 2 cut(s) 115, 1270
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 2 cut(s) 612, 1175
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 6 cut(s) 317, 572, 641, 786, 790, 800
CciI TCATGA 1 cut(s) 808
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 207
CseI GACGC 1 cut(s) 17
Csp6I GTAC 2 cut(s) 1129, 1182
CviAII CATG 9 cut(s) 225, 316, 423, 542, 737, 809, 854, 940, 1297
CviQI GTAC 2 cut(s) 1129, 1182
DdeI CTNAG 5 cut(s) 397, 453, 1125, 1229, 1419
DpnI GATC 3 cut(s) 495, 978, 1314
DpnII GATC 3 cut(s) 493, 976, 1312
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
Eco130I CCWWGG 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 1346
EcoRI GAATTC 1 cut(s) 687
EcoRII CCWGG 2 cut(s) 147, 237
EcoT14I CCWWGG 1 cut(s) 93
EcoT22I ATGCAT 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 9 cut(s) 228, 319, 426, 545, 740, 812, 857, 943, 1300
FaqI GGGAC 2 cut(s) 20, 1386
FatI CATG 9 cut(s) 224, 315, 422, 541, 736, 808, 853, 939, 1296
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 3 cut(s) 153, 177, 618
FokI GGATG 2 cut(s) 122, 1257
Fsp4HI GCNGC 3 cut(s) 153, 177, 618
FspBI CTAG 3 cut(s) 341, 785, 795
GlaI GCGC 1 cut(s) 291
GluI GCNGC 3 cut(s) 153, 177, 618
GsuI CTGGAG 1 cut(s) 12
HaeIII GGCC 2 cut(s) 35, 209
HapII CCGG 1 cut(s) 1111
HgaI GACGC 1 cut(s) 17
HhaI GCGC 1 cut(s) 292
Hin1II CATG 9 cut(s) 228, 319, 426, 545, 740, 812, 857, 943, 1300
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 1294
HindII GTYRAC 1 cut(s) 1294
HinfI GANTC 8 cut(s) 21, 70, 133, 234, 467, 858, 1117, 1255
HpaII CCGG 1 cut(s) 1111
HphI GGTGA 3 cut(s) 154, 1112, 1333
Hpy166II GTNNAC 1 cut(s) 1294
Hpy188I TCNGA 8 cut(s) 370, 400, 418, 430, 442, 454, 1126, 1254
Hpy188III TCNNGA 4 cut(s) 29, 491, 809, 1219
Hpy8I GTNNAC 1 cut(s) 1294
HpyAV CCTTC 3 cut(s) 304, 908, 1091
HpyCH4III ACNGT 4 cut(s) 190, 361, 898, 992
HpyCH4IV ACGT 1 cut(s) 1277
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 144, 781, 884
HpyF3I CTNAG 5 cut(s) 397, 453, 1125, 1229, 1419
HpySE526I ACGT 1 cut(s) 1277
Hsp92II CATG 9 cut(s) 228, 319, 426, 545, 740, 812, 857, 943, 1300
HspAI GCGC 1 cut(s) 290
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 3 cut(s) 493, 976, 1312
LguI GCTCTTC 1 cut(s) 540
LmnI GCTCC 1 cut(s) 703
Lsp1109I GCAGC 3 cut(s) 163, 164, 604
LweI GCATC 3 cut(s) 20, 409, 865
MaeI CTAG 3 cut(s) 341, 785, 795
MaeII ACGT 1 cut(s) 1277
MaeIII GTNAC 2 cut(s) 257, 1106
MalI GATC 3 cut(s) 495, 978, 1314
MboI GATC 3 cut(s) 493, 976, 1312
MboII GAAGA 7 cut(s) 294, 557, 670, 954, 1126, 1247, 1313
MhlI GDGCHC 1 cut(s) 218
MluCI AATT 8 cut(s) 126, 248, 412, 497, 582, 687, 891, 933
MlyI GAGTC 3 cut(s) 142, 867, 1249
MmeI TCCRAC 3 cut(s) 420, 1232, 1298
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 691
MseI TTAA 4 cut(s) 387, 408, 462, 750
MspI CCGG 1 cut(s) 1111
MspR9I CCNGG 2 cut(s) 149, 239
Mva1269I GAATGC 1 cut(s) 1367
MvaI CCWGG 2 cut(s) 149, 239
MvnI CGCG 2 cut(s) 16, 138
MwoI GCNNNNNNNGC 4 cut(s) 96, 144, 781, 884
NdeII GATC 3 cut(s) 493, 976, 1312
NheI GCTAGC 1 cut(s) 784
NlaIII CATG 9 cut(s) 228, 319, 426, 545, 740, 812, 857, 943, 1300
NlaIV GGNNCC 2 cut(s) 489, 1196
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 2 cut(s) 319, 426
NspV TTCGAA 2 cut(s) 124, 280
PaeI GCATGC 1 cut(s) 319
PagI TCATGA 1 cut(s) 808
PciSI GCTCTTC 1 cut(s) 540
PctI GAATGC 1 cut(s) 1367
PdmI GAANNNNTTC 1 cut(s) 691
PfeI GAWTC 5 cut(s) 21, 70, 234, 467, 1117
PflFI GACNNNGTC 1 cut(s) 61
PkrI GCNGC 3 cut(s) 154, 178, 619
PleI GAGTC 3 cut(s) 141, 866, 1249
PpsI GAGTC 3 cut(s) 141, 866, 1249
Psp6I CCWGG 2 cut(s) 147, 237
PspGI CCWGG 2 cut(s) 147, 237
PspN4I GGNNCC 2 cut(s) 489, 1196
PspPI GGNCC 1 cut(s) 207
PstI CTGCAG 1 cut(s) 108
PsyI GACNNNGTC 1 cut(s) 61
RsaI GTAC 2 cut(s) 1130, 1183
RsaNI GTAC 2 cut(s) 1129, 1182
SapI GCTCTTC 1 cut(s) 540
SaqAI TTAA 4 cut(s) 387, 408, 462, 750
SatI GCNGC 3 cut(s) 153, 177, 618
Sau3AI GATC 3 cut(s) 493, 976, 1312
Sau96I GGNCC 1 cut(s) 207
SchI GAGTC 3 cut(s) 142, 867, 1249
ScrFI CCNGG 2 cut(s) 149, 239
SduI GDGCHC 1 cut(s) 218
SfaNI GCATC 3 cut(s) 20, 409, 865
SfcI CTRYAG 1 cut(s) 104
SfuI TTCGAA 2 cut(s) 124, 280
SmlI CTYRAG 4 cut(s) 202, 707, 828, 1133
SmoI CTYRAG 4 cut(s) 202, 707, 828, 1133
SpeI ACTAGT 1 cut(s) 340
SphI GCATGC 1 cut(s) 319
Sse9I AATT 8 cut(s) 126, 248, 412, 497, 582, 687, 891, 933
SsiI CCGC 1 cut(s) 196
SspMI CTAG 3 cut(s) 341, 785, 795
StyD4I CCNGG 2 cut(s) 147, 237
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 4 cut(s) 190, 361, 898, 992
TaiI ACGT 1 cut(s) 1280
TaqI TCGA 3 cut(s) 124, 280, 873
TasI AATT 8 cut(s) 126, 248, 412, 497, 582, 687, 891, 933
TatI WGTACW 1 cut(s) 1181
TfiI GAWTC 5 cut(s) 21, 70, 234, 467, 1117
Tru1I TTAA 4 cut(s) 387, 408, 462, 750
Tru9I TTAA 4 cut(s) 387, 408, 462, 750
TscAI CASTG 2 cut(s) 619, 1175
TseI GCWGC 3 cut(s) 152, 176, 617
TspDTI ATGAA 5 cut(s) 62, 387, 558, 1224, 1351
TspGWI ACGGA 2 cut(s) 255, 699
TspRI CASTG 2 cut(s) 619, 1175
Tth111I GACNNNGTC 1 cut(s) 61
XapI RAATTY 2 cut(s) 687, 933
XceI RCATGY 2 cut(s) 319, 426
XcmI CCANNNNNNNNNTGG 1 cut(s) 851
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 3 cut(s) 341, 785, 795
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.