pycom03g09210

Transcriptional activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0008252
Physical Location & Seq
Reverse (-)
7683797 .. 7685232
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGTTGCAAACTACAACAGGAGAGAAAGATAGCAATCATTTGAAGAGAAAAAGAGAGGAAAAAAAAAAAAAAAGAAAAGAAAAGAGGGAGAGAAGTAAGGGTGTTGCAGCTGCAGAGGAACCAATGGCGACGCCGAAACAGATGTGGGAGCAGCAGCAATTGCAGGTGCAGCTAGTGAAGAACTCCGGTATCGTCAGTTACAATGGGAGTCCGGTAGCTGATGACAAGGAAGAAGAGATGTCAAGATCTGCATTGGCTTTGTTCAAAGCGAAGGAGGAAGAGATTGAGAGGAAGAAGTTGGAGGTGAAAGACAAGGTTCAGGCTCAGCTGGGACGCGTCGAGGAAGCAACTAAGCGCTTGTCTGAGATTCAGGAAGAGCTTGAAACGCTGATAGATCCAATGAGAAAGGAAGTTGCAATTATTCGCAAGAGGATAGACATCATTAACAAAGAGTTAAAGCCGCTAGGTCAGAGCTGCCAGAGGAAGGAGAAAGAATACAAGGAAGCTCTTGAGGCTTTAAACGAGAAGAGCAGAGAGAAATCTCAACTAGTTTCCAAATTGATGGAGCTTGTGGGTGAAAGTGAGAGAATGAGGATAAAGAAGCTGGAGGAGCTGAACAAACATGTTGAAAATCTGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04115 map04115
Pfam Domains
Protein Families
Transcrip_act

Protein Analysis

214

Amino Acids

24.91

Weight (kDa)

9.34

Isoelectric Point (pI)

57.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transcrip_act PF04949 60 - 210 5.2e-71 Transcriptional activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 154
Acc36I ACCTGC 1 cut(s) 154
AccII CGCG 1 cut(s) 336
AciI CCGC 1 cut(s) 461
AclWI GGATC 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 131
AfeI AGCGCT 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 484
AflIII ACRYGT 2 cut(s) 334, 622
AgsI TTSAA 4 cut(s) 43, 265, 383, 629
AhlI ACTAGT 1 cut(s) 547
AlwI GGATC 1 cut(s) 389
Aor51HI AGCGCT 1 cut(s) 356
ApeKI GCWGC 6 cut(s) 107, 110, 151, 154, 169, 474
AspLEI GCGC 1 cut(s) 357
AsuHPI GGTGA 2 cut(s) 316, 587
BaeI ACNNNNGTAYC 2 cut(s) 172, 205
BbvI GCAGC 6 cut(s) 97, 119, 163, 166, 181, 461
BccI CCATC 1 cut(s) 556
BcuI ACTAGT 1 cut(s) 547
BfaI CTAG 3 cut(s) 173, 464, 548
BfmI CTRYAG 1 cut(s) 111
BfoI RGCGCY 1 cut(s) 358
BfuAI ACCTGC 1 cut(s) 154
BglII AGATCT 1 cut(s) 245
BisI GCNGC 7 cut(s) 108, 111, 152, 155, 170, 461, 475
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 7 cut(s) 109, 112, 153, 156, 171, 462, 476
BmiI GGNNCC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 626
Bpu1102I GCTNAGC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 530
BsaBI GATNNNNATC 2 cut(s) 33, 437
BsaHI GRCGYC 1 cut(s) 131
BsaWI WCCGGW 2 cut(s) 185, 211
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse8I GATNNNNATC 2 cut(s) 33, 437
BseJI GATNNNNATC 2 cut(s) 33, 437
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMII CTCAG 2 cut(s) 338, 354
BseRI GAGGAG 1 cut(s) 623
BseXI GCAGC 6 cut(s) 97, 119, 163, 166, 181, 461
BseYI CCCAGC 1 cut(s) 328
BsgI GTGCAG 1 cut(s) 188
Bsh1236I CGCG 1 cut(s) 336
BsiSI CCGG 2 cut(s) 186, 212
BslFI GGGAC 1 cut(s) 345
BslI CCNNNNNNNGG 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 345
Bsp143I GATC 2 cut(s) 245, 394
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 1 cut(s) 461
BspCNI CTCAG 2 cut(s) 337, 355
BspFNI CGCG 1 cut(s) 336
BspLI GGNNCC 1 cut(s) 120
BspMAI CTGCAG 1 cut(s) 115
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 1 cut(s) 389
BspQI GCTCTTC 2 cut(s) 369, 521
BssMI GATC 2 cut(s) 245, 394
BssNI GRCGYC 1 cut(s) 131
Bst6I CTCTTC 5 cut(s) 38, 228, 273, 369, 521
BstACI GRCGYC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 160
BstDEI CTNAG 3 cut(s) 324, 351, 363
BstFNI CGCG 1 cut(s) 336
BstH2I RGCGCY 1 cut(s) 358
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 2 cut(s) 248, 397
BstMBI GATC 2 cut(s) 245, 394
BstMWI GCNNNNNNNGC 5 cut(s) 160, 169, 385, 512, 610
BstNSI RCATGY 1 cut(s) 626
BstSFI CTRYAG 1 cut(s) 111
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 6 cut(s) 97, 119, 163, 166, 181, 461
BstX2I RGATCY 2 cut(s) 245, 394
BstXI CCANNNNNNTGG 1 cut(s) 562
BstYI RGATCY 2 cut(s) 245, 394
BveI ACCTGC 1 cut(s) 154
CfoI GCGC 1 cut(s) 357
CseI GACGC 3 cut(s) 139, 325, 342
CviAII CATG 1 cut(s) 623
DdeI CTNAG 3 cut(s) 324, 351, 363
DpnI GATC 2 cut(s) 247, 396
DpnII GATC 2 cut(s) 245, 394
DraI TTTAAA 1 cut(s) 519
Eam1104I CTCTTC 5 cut(s) 38, 228, 273, 369, 521
EarI CTCTTC 5 cut(s) 38, 228, 273, 369, 521
Eco47III AGCGCT 1 cut(s) 356
FaeI CATG 1 cut(s) 626
FaiI YATR 1 cut(s) 624
FaqI GGGAC 1 cut(s) 345
FatI CATG 1 cut(s) 622
Fnu4HI GCNGC 7 cut(s) 108, 111, 152, 155, 170, 461, 475
Fsp4HI GCNGC 7 cut(s) 108, 111, 152, 155, 170, 461, 475
FspBI CTAG 3 cut(s) 173, 464, 548
GlaI GCGC 1 cut(s) 356
GluI GCNGC 7 cut(s) 108, 111, 152, 155, 170, 461, 475
GsaI CCCAGC 1 cut(s) 332
GsuI CTGGAG 1 cut(s) 626
HaeII RGCGCY 1 cut(s) 358
HapII CCGG 2 cut(s) 186, 212
HgaI GACGC 3 cut(s) 139, 325, 342
HhaI GCGC 1 cut(s) 357
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 1 cut(s) 626
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HinfI GANTC 2 cut(s) 208, 367
HpaII CCGG 2 cut(s) 186, 212
HphI GGTGA 2 cut(s) 316, 587
Hpy188I TCNGA 2 cut(s) 364, 471
Hpy188III TCNNGA 3 cut(s) 243, 371, 509
Hpy99I CGWCG 2 cut(s) 133, 341
HpyAV CCTTC 2 cut(s) 265, 478
HpyCH4V TGCA 7 cut(s) 7, 107, 113, 163, 169, 251, 416
HpyF10VI GCNNNNNNNGC 5 cut(s) 160, 169, 385, 512, 610
HpyF3I CTNAG 3 cut(s) 324, 351, 363
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 1 cut(s) 626
HspAI GCGC 1 cut(s) 355
Kzo9I GATC 2 cut(s) 245, 394
LguI GCTCTTC 2 cut(s) 369, 521
LmnI GCTCC 3 cut(s) 148, 565, 610
LpnPI CCDG 9 cut(s) 3, 149, 199, 225, 305, 314, 356, 491, 590
Lsp1109I GCAGC 6 cut(s) 97, 119, 163, 166, 181, 461
MaeI CTAG 3 cut(s) 173, 464, 548
MaeIII GTNAC 1 cut(s) 197
MalI GATC 2 cut(s) 247, 396
MboI GATC 2 cut(s) 245, 394
MboII GAAGA 8 cut(s) 55, 190, 242, 245, 290, 304, 386, 538
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 2 cut(s) 245, 394
MluCI AATT 3 cut(s) 158, 417, 557
MluI ACGCGT 1 cut(s) 334
MlyI GAGTC 1 cut(s) 217
MmeI TCCRAC 1 cut(s) 279
MseI TTAA 4 cut(s) 444, 455, 518, 640
MspA1I CMGCKG 2 cut(s) 110, 328
MspI CCGG 2 cut(s) 186, 212
MunI CAATTG 1 cut(s) 158
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 5 cut(s) 160, 169, 385, 512, 610
NdeII GATC 2 cut(s) 245, 394
NlaIII CATG 1 cut(s) 626
NlaIV GGNNCC 1 cut(s) 120
NspI RCATGY 1 cut(s) 626
PaqCI CACCTGC 1 cut(s) 154
PciI ACATGT 1 cut(s) 622
PciSI GCTCTTC 2 cut(s) 369, 521
PfeI GAWTC 1 cut(s) 367
PkrI GCNGC 7 cut(s) 109, 112, 153, 156, 171, 462, 476
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
PscI ACATGT 1 cut(s) 622
PspFI CCCAGC 1 cut(s) 328
PspN4I GGNNCC 1 cut(s) 120
PstI CTGCAG 1 cut(s) 115
PsuI RGATCY 2 cut(s) 245, 394
PvuII CAGCTG 2 cut(s) 110, 328
SapI GCTCTTC 2 cut(s) 369, 521
SaqAI TTAA 4 cut(s) 444, 455, 518, 640
SatI GCNGC 7 cut(s) 108, 111, 152, 155, 170, 461, 475
Sau3AI GATC 2 cut(s) 245, 394
SchI GAGTC 1 cut(s) 217
SfcI CTRYAG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
SpeI ACTAGT 1 cut(s) 547
Sse9I AATT 3 cut(s) 158, 417, 557
SsiI CCGC 1 cut(s) 461
SspMI CTAG 3 cut(s) 173, 464, 548
TaqI TCGA 1 cut(s) 339
TasI AATT 3 cut(s) 158, 417, 557
TauI GCSGC 1 cut(s) 463
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 4 cut(s) 444, 455, 518, 640
Tru9I TTAA 4 cut(s) 444, 455, 518, 640
TseI GCWGC 6 cut(s) 107, 110, 151, 154, 169, 474
XceI RCATGY 1 cut(s) 626
XspI CTAG 3 cut(s) 173, 464, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.