pycom03g10340

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003236
Physical Location & Seq
Forward (+)
9489461 .. 9494536
5076 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1614 bp
ATGACTCGAATTCTGGTTCAGCGAGGTTCTTCAAGTGGTTCTTCTTCCAACCCAACTCGTCCTTCTTCCTTGTCTGGGTCTTCTTCTGCTTGGGCACAGTCACAGGTTAATAATCCTCAGGCTCTTTCGGTTGTGAAAGACGAGGAAATTGTAGAGGAGGCACCAGAAAATGTTTTCGTGGATGAGCTGACAGAGTATTGTGGGACTGGTGACAATAAGGCCGTGAAAAATGATGATCTTTTGACAGAAAGCTGTAATGATGACAATGTGAGTTCAAATGATGAATTAGCTGAGGGGGAAAAAGTTGGTAAAAATGACATTGCCATTCCTGGGGAATTGATGAAAGGTTTAGACAGGGTGAGGATTTCTGAAAGGGTGACAGTTGAAAATGTGGACTCGAGCAGTGATTCTTTACAAAATGTAATTGCGAGTTCTCAGCCACCACCTCCTCCAGTCCCTCCCCCAAAACTTTCCTCAGCAAACTCGAATTCAAGGAGATCTGTCTCAGGGAGTTCAAATGCTGTCCGGATTGGACCTTCTAGAAGAGCAGCTGCCTGGCCTGTTGTTTCAACTAGGACTTCGCCGAATGGGTCGCTGCCCTCTTCTCCCAGGTCTCATGGTGAAAGCGAGGGGTACAATAGTGCTGATGAGCAAAGTCCTTGCTTTGTATCTTCCTATGATGACACTGAAAGAGAACGACAGTTTGAAATTGATATTAGGCGTGCAAAAGGTTTTGAAGTGAAAAGAATGCTGGAGGATGGGAATTGTCTCTTTCGTGCTGTTGCAGATCAAGTTTATGGGGATTCTGATGCCTGTGACTTGATCCGGCAAATGTGCATAGAGTACATGGAACGAGAGAGGGATCACTTTTCTCAATTTATTACAGAAGGATTTACCTCTTATTGTAAGAGGAAAAGAAGAGACAAGGTCTATGGAAACAATATTGAGATCCAAGCCTTATCTGAAATGTATAATCGGCCCATCCATATTTATTCCTATAGCACAGAACCGATTAATATTTTCCATGGAAGCTATGACACTGACACACCACCCCTACGACTAAGTTATCATCATGGAAATCATTACAACTCTCTTGTTCGATCCCCGTCGTCAGACCATTGGTGTAGGACTTGGGTTCAGCCGTCTTCGTGGGTTGTTTCAAATGTTGACAAGGATCAGGTCAAGGCTGCTATTAAGGCTCAACAGGACCAACAAATTGATAATGCACTTCTAGCTGAGGGGCGATTTTACTCGGATCTGGAGCTTACTGAGAAAGAAATTGAGCGCATGGTTATGGAAGCTTCGAGGCTTGAATATGCAGACGGCATATTTAATCAACAACTTGGACGTAGAGAATCTTCCACTTCTAGTGCTGAGCCATCATCTTCTGGAGCTACAGGATCATCAGGCAGTGATACTAAAGTTGAAGGGAGGGACCAAGGATTTCAGGATTCGACCCTTAGCAGTAGTGTGCAAGTTGTGCTATCGATGGGGTTCAGCTATCTGCAGGTGATTGAGGCTTACAGCATATTTGGAGATGATGTCGATTCCATGGTTTGTTACCTGTTGGAAACTAGCAGCAGCAGCCGGCGCAAAGGCAAGGCAACCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04622 map04622
Pfam Domains
Protein Families
OTU

Protein Analysis

538

Amino Acids

59.36

Weight (kDa)

4.88

Isoelectric Point (pI)

62.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 253 - 362 1.1e-18 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1498
Acc36I ACCTGC 1 cut(s) 1498
AccB1I GGYRCC 1 cut(s) 160
AccIII TCCGGA 1 cut(s) 525
AclWI GGATC 7 cut(s) 817, 870, 943, 1095, 1182, 1263, 1408
AcsI RAATTY 2 cut(s) 9, 487
AfaI GTAC 2 cut(s) 635, 845
AfiI CCNNNNNNNGG 2 cut(s) 75, 330
AjnI CCWGG 3 cut(s) 328, 554, 608
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
Alw26I GTCTC 4 cut(s) 508, 618, 773, 915
AlwI GGATC 7 cut(s) 817, 870, 943, 1095, 1182, 1263, 1408
Ama87I CYCGRG 1 cut(s) 397
Aor13HI TCCGGA 1 cut(s) 525
AoxI GGCC 3 cut(s) 219, 557, 977
ApeKI GCWGC 7 cut(s) 548, 551, 595, 1187, 1578, 1581, 1584
ApoI RAATTY 2 cut(s) 9, 487
AseI ATTAAT 1 cut(s) 1014
AspLEI GCGC 2 cut(s) 1287, 1593
AspS9I GGNCC 4 cut(s) 533, 978, 1207, 1435
AsuHPI GGTGA 5 cut(s) 221, 370, 388, 632, 1522
AvaI CYCGRG 1 cut(s) 397
AvaII GGWCC 3 cut(s) 533, 1207, 1435
AxyI CCTNAGG 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 97
BaeI ACNNNNGTAYC 4 cut(s) 625, 658, 1407, 1440
BanI GGYRCC 1 cut(s) 160
BarI GAAGNNNNNNTAC 2 cut(s) 1342, 1374
BbsI GAAGAC 2 cut(s) 72, 1137
BbvCI CCTCAGC 3 cut(s) 291, 475, 1236
BbvI GCAGC 7 cut(s) 538, 560, 582, 1174, 1590, 1593, 1596
BccI CCATC 4 cut(s) 752, 989, 1387, 1483
BceAI ACGGC 3 cut(s) 206, 1126, 1339
BciT130I CCWGG 3 cut(s) 330, 556, 610
BcoDI GTCTC 4 cut(s) 508, 618, 773, 915
BfaI CTAG 5 cut(s) 540, 573, 1232, 1368, 1575
BfmI CTRYAG 3 cut(s) 997, 1395, 1505
BfuAI ACCTGC 1 cut(s) 1498
BglII AGATCT 1 cut(s) 497
BisI GCNGC 7 cut(s) 549, 552, 596, 1188, 1579, 1582, 1585
BlpI GCTNAGC 1 cut(s) 1374
BlsI GCNGC 7 cut(s) 550, 553, 597, 1189, 1580, 1583, 1586
Bme1390I CCNGG 3 cut(s) 330, 556, 610
Bme18I GGWCC 3 cut(s) 533, 1207, 1435
BmeT110I CYCGRG 1 cut(s) 397
BmgT120I GGNCC 4 cut(s) 533, 978, 1207, 1435
BmiI GGNNCC 2 cut(s) 162, 1436
BmrFI CCNGG 3 cut(s) 330, 556, 610
BmsI GCATC 1 cut(s) 799
BpiI GAAGAC 2 cut(s) 72, 1137
BpmI CTGGAG 4 cut(s) 435, 773, 1280, 1410
Bpu10I CCTNAGC 4 cut(s) 291, 475, 1236, 1460
Bpu1102I GCTNAGC 1 cut(s) 1374
Bsa29I ATCGAT 1 cut(s) 1487
BsaI GGTCTC 1 cut(s) 618
BsaJI CCNNGG 5 cut(s) 329, 608, 1024, 1438, 1551
BsaWI WCCGGW 1 cut(s) 525
BsaXI ACNNNNNCTCC 2 cut(s) 1527, 1557
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 330
Bse118I RCCGGY 1 cut(s) 1587
Bse1I ACTGG 2 cut(s) 211, 452
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 318
BseAI TCCGGA 1 cut(s) 525
BseBI CCWGG 3 cut(s) 330, 556, 610
BseCI ATCGAT 1 cut(s) 1487
BseDI CCNNGG 5 cut(s) 329, 608, 1024, 1438, 1551
BseGI GGATG 3 cut(s) 187, 763, 981
BseLI CCNNNNNNNGG 2 cut(s) 75, 330
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 8 cut(s) 131, 282, 449, 489, 519, 1227, 1260, 1365
BseNI ACTGG 2 cut(s) 211, 452
BseRI GAGGAG 2 cut(s) 170, 438
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 7 cut(s) 538, 560, 582, 1174, 1590, 1593, 1596
BshFI GGCC 3 cut(s) 221, 559, 979
BshNI GGYRCC 1 cut(s) 160
BshVI ATCGAT 1 cut(s) 1487
BsiHKCI CYCGRG 1 cut(s) 397
BsiSI CCGG 3 cut(s) 526, 826, 1588
BslFI GGGAC 3 cut(s) 217, 440, 1448
BslI CCNNNNNNNGG 2 cut(s) 75, 330
BsmAI GTCTC 4 cut(s) 508, 618, 773, 915
BsmFI GGGAC 3 cut(s) 217, 440, 1448
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 3 cut(s) 221, 559, 979
Bso31I GGTCTC 1 cut(s) 618
BsoBI CYCGRG 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 97
Bsp13I TCCGGA 1 cut(s) 525
Bsp1720I GCTNAGC 1 cut(s) 1374
Bsp19I CCATGG 2 cut(s) 1024, 1551
BspANI GGCC 3 cut(s) 221, 559, 979
BspCNI CTCAG 8 cut(s) 130, 283, 448, 488, 518, 1228, 1261, 1366
BspDI ATCGAT 1 cut(s) 1487
BspEI TCCGGA 1 cut(s) 525
BspLI GGNNCC 2 cut(s) 162, 1436
BspMAI CTGCAG 1 cut(s) 1509
BspMI ACCTGC 1 cut(s) 1498
BspPI GGATC 7 cut(s) 817, 870, 943, 1095, 1182, 1263, 1408
BspQI GCTCTTC 1 cut(s) 538
BspT107I GGYRCC 1 cut(s) 160
BspTNI GGTCTC 1 cut(s) 618
BsrDI GCAATG 1 cut(s) 318
BsrFI RCCGGY 1 cut(s) 1587
BsrI ACTGG 2 cut(s) 211, 452
BssAI RCCGGY 1 cut(s) 1587
BssECI CCNNGG 5 cut(s) 329, 608, 1024, 1438, 1551
BssT1I CCWWGG 3 cut(s) 1024, 1438, 1551
Bst2UI CCWGG 3 cut(s) 330, 556, 610
Bst4CI ACNGT 3 cut(s) 99, 382, 702
Bst6I CTCTTC 3 cut(s) 538, 607, 913
BstAPI GCANNNNNTGC 1 cut(s) 1480
BstC8I GCNNGC 2 cut(s) 723, 1589
BstDSI CCRYGG 2 cut(s) 1024, 1551
BstF5I GGATG 3 cut(s) 187, 763, 981
BstHHI GCGC 2 cut(s) 1287, 1593
BstMAI GTCTC 4 cut(s) 508, 618, 773, 915
BstMWI GCNNNNNNNGC 5 cut(s) 1196, 1232, 1480, 1584, 1590
BstNI CCWGG 3 cut(s) 330, 556, 610
BstSCI CCNGG 3 cut(s) 328, 554, 608
BstSFI CTRYAG 3 cut(s) 997, 1395, 1505
BstSLI GKGCMC 1 cut(s) 97
BstV1I GCAGC 7 cut(s) 538, 560, 582, 1174, 1590, 1593, 1596
BstV2I GAAGAC 2 cut(s) 72, 1137
BstX2I RGATCY 3 cut(s) 497, 948, 1255
BstYI RGATCY 3 cut(s) 497, 948, 1255
Bsu15I ATCGAT 1 cut(s) 1487
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 3 cut(s) 221, 559, 979
BsuTUI ATCGAT 1 cut(s) 1487
BtgI CCRYGG 2 cut(s) 1024, 1551
BtsCI GGATG 3 cut(s) 187, 763, 981
BtsI GCAGTG 2 cut(s) 409, 1417
BtsIMutI CAGTG 4 cut(s) 409, 684, 1038, 1417
BveI ACCTGC 1 cut(s) 1498
Cac8I GCNNGC 2 cut(s) 723, 1589
CfoI GCGC 2 cut(s) 1287, 1593
Cfr10I RCCGGY 1 cut(s) 1587
Cfr13I GGNCC 4 cut(s) 533, 978, 1207, 1435
ClaI ATCGAT 1 cut(s) 1487
Csp6I GTAC 2 cut(s) 634, 844
CviAII CATG 6 cut(s) 617, 847, 1025, 1073, 1288, 1552
CviQI GTAC 2 cut(s) 634, 844
Eam1104I CTCTTC 3 cut(s) 538, 607, 913
EarI CTCTTC 3 cut(s) 538, 607, 913
Eco130I CCWWGG 3 cut(s) 1024, 1438, 1551
Eco31I GGTCTC 1 cut(s) 618
Eco47I GGWCC 3 cut(s) 533, 1207, 1435
Eco81I CCTNAGG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 397
EcoRI GAATTC 2 cut(s) 9, 487
EcoRII CCWGG 3 cut(s) 328, 554, 608
EcoT14I CCWWGG 3 cut(s) 1024, 1438, 1551
ErhI CCWWGG 3 cut(s) 1024, 1438, 1551
FaeI CATG 6 cut(s) 620, 850, 1028, 1076, 1291, 1555
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FaqI GGGAC 3 cut(s) 217, 440, 1448
FatI CATG 6 cut(s) 616, 846, 1024, 1072, 1287, 1551
Fnu4HI GCNGC 7 cut(s) 549, 552, 596, 1188, 1579, 1582, 1585
FokI GGATG 3 cut(s) 194, 770, 968
Fsp4HI GCNGC 7 cut(s) 549, 552, 596, 1188, 1579, 1582, 1585
FspBI CTAG 5 cut(s) 540, 573, 1232, 1368, 1575
GlaI GCGC 2 cut(s) 1286, 1592
GluI GCNGC 7 cut(s) 549, 552, 596, 1188, 1579, 1582, 1585
GsuI CTGGAG 4 cut(s) 435, 773, 1280, 1410
HaeIII GGCC 3 cut(s) 221, 559, 979
HapII CCGG 3 cut(s) 526, 826, 1588
HhaI GCGC 2 cut(s) 1287, 1593
Hin1II CATG 6 cut(s) 620, 850, 1028, 1076, 1291, 1555
Hin6I GCGC 2 cut(s) 1285, 1591
HinP1I GCGC 2 cut(s) 1285, 1591
HincII GTYRAC 1 cut(s) 1168
HindII GTYRAC 1 cut(s) 1168
HindIII AAGCTT 1 cut(s) 1299
HinfI GANTC 7 cut(s) 4, 395, 407, 803, 1355, 1451, 1547
HpaII CCGG 3 cut(s) 526, 826, 1588
HphI GGTGA 5 cut(s) 221, 370, 388, 632, 1522
Hpy166II GTNNAC 2 cut(s) 394, 1168
Hpy188I TCNGA 5 cut(s) 370, 808, 964, 1114, 1255
Hpy188III TCNNGA 5 cut(s) 526, 540, 1259, 1389, 1448
Hpy8I GTNNAC 2 cut(s) 394, 1168
Hpy99I CGWCG 1 cut(s) 1111
HpyAV CCTTC 4 cut(s) 72, 546, 881, 1421
HpyCH4III ACNGT 3 cut(s) 99, 382, 702
HpyCH4IV ACGT 1 cut(s) 1348
HpyCH4V TGCA 7 cut(s) 725, 785, 837, 1226, 1319, 1474, 1507
HpyF10VI GCNNNNNNNGC 5 cut(s) 1196, 1232, 1480, 1584, 1590
HpySE526I ACGT 1 cut(s) 1348
Hsp92II CATG 6 cut(s) 620, 850, 1028, 1076, 1291, 1555
HspAI GCGC 2 cut(s) 1285, 1591
Kpn2I TCCGGA 1 cut(s) 525
KroI GCCGGC 1 cut(s) 1587
KroNI GCCGGC 1 cut(s) 1589
LguI GCTCTTC 1 cut(s) 538
LmnI GCTCC 2 cut(s) 1261, 1391
Lsp1109I GCAGC 7 cut(s) 538, 560, 582, 1174, 1590, 1593, 1596
LweI GCATC 1 cut(s) 799
MaeI CTAG 5 cut(s) 540, 573, 1232, 1368, 1575
MaeII ACGT 1 cut(s) 1348
MaeIII GTNAC 5 cut(s) 99, 209, 376, 815, 1559
MflI RGATCY 3 cut(s) 497, 948, 1255
MhlI GDGCHC 1 cut(s) 97
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 2 cut(s) 72, 1548
MroI TCCGGA 1 cut(s) 525
MroNI GCCGGC 1 cut(s) 1587
MseI TTAA 4 cut(s) 108, 1014, 1194, 1332
MslI CAYNNNNRTG 1 cut(s) 1292
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 3 cut(s) 526, 826, 1588
MspR9I CCNGG 3 cut(s) 330, 556, 610
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 3 cut(s) 330, 556, 610
MwoI GCNNNNNNNGC 5 cut(s) 1196, 1232, 1480, 1584, 1590
NaeI GCCGGC 1 cut(s) 1589
NcoI CCATGG 2 cut(s) 1024, 1551
NgoMIV GCCGGC 1 cut(s) 1587
NlaIII CATG 6 cut(s) 620, 850, 1028, 1076, 1291, 1555
NlaIV GGNNCC 2 cut(s) 162, 1436
NmuCI GTSAC 4 cut(s) 99, 209, 376, 815
PaeR7I CTCGAG 1 cut(s) 397
PaqCI CACCTGC 1 cut(s) 1498
PciSI GCTCTTC 1 cut(s) 538
PctI GAATGC 1 cut(s) 753
PdiI GCCGGC 1 cut(s) 1589
PfeI GAWTC 5 cut(s) 407, 803, 1355, 1451, 1547
PflFI GACNNNGTC 1 cut(s) 926
PkrI GCNGC 7 cut(s) 550, 553, 597, 1189, 1580, 1583, 1586
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
PshBI ATTAAT 1 cut(s) 1014
Psp6I CCWGG 3 cut(s) 328, 554, 608
PspGI CCWGG 3 cut(s) 328, 554, 608
PspN4I GGNNCC 2 cut(s) 162, 1436
PspPI GGNCC 4 cut(s) 533, 978, 1207, 1435
PspXI VCTCGAGB 1 cut(s) 397
PstI CTGCAG 1 cut(s) 1509
PsuI RGATCY 3 cut(s) 497, 948, 1255
PsyI GACNNNGTC 1 cut(s) 926
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 2 cut(s) 635, 845
RsaNI GTAC 2 cut(s) 634, 844
RseI CAYNNNNRTG 1 cut(s) 1292
SapI GCTCTTC 1 cut(s) 538
SaqAI TTAA 4 cut(s) 108, 1014, 1194, 1332
SatI GCNGC 7 cut(s) 549, 552, 596, 1188, 1579, 1582, 1585
Sau96I GGNCC 4 cut(s) 533, 978, 1207, 1435
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 3 cut(s) 330, 556, 610
SduI GDGCHC 1 cut(s) 97
SfaNI GCATC 1 cut(s) 799
SfcI CTRYAG 3 cut(s) 997, 1395, 1505
Sfr274I CTCGAG 1 cut(s) 397
SinI GGWCC 3 cut(s) 533, 1207, 1435
SlaI CTCGAG 1 cut(s) 397
SmiMI CAYNNNNRTG 1 cut(s) 1292
SmlI CTYRAG 1 cut(s) 397
SmoI CTYRAG 1 cut(s) 397
SspI AATATT 2 cut(s) 943, 1018
SspMI CTAG 5 cut(s) 540, 573, 1232, 1368, 1575
StyD4I CCNGG 3 cut(s) 328, 554, 608
StyI CCWWGG 3 cut(s) 1024, 1438, 1551
TaaI ACNGT 3 cut(s) 99, 382, 702
TaiI ACGT 1 cut(s) 1351
TaqI TCGA 8 cut(s) 7, 398, 485, 1099, 1304, 1454, 1487, 1545
TatI WGTACW 1 cut(s) 843
TfiI GAWTC 5 cut(s) 407, 803, 1355, 1451, 1547
Tru1I TTAA 4 cut(s) 108, 1014, 1194, 1332
Tru9I TTAA 4 cut(s) 108, 1014, 1194, 1332
TscAI CASTG 4 cut(s) 409, 691, 1045, 1417
TseFI GTSAC 4 cut(s) 99, 209, 376, 815
TseI GCWGC 7 cut(s) 548, 551, 595, 1187, 1578, 1581, 1584
Tsp45I GTSAC 4 cut(s) 99, 209, 376, 815
TspDTI ATGAA 2 cut(s) 297, 356
TspRI CASTG 4 cut(s) 409, 691, 1045, 1417
Tth111I GACNNNGTC 1 cut(s) 926
VpaK11BI GGWCC 3 cut(s) 533, 1207, 1435
VspI ATTAAT 1 cut(s) 1014
XapI RAATTY 2 cut(s) 9, 487
XbaI TCTAGA 1 cut(s) 539
XhoI CTCGAG 1 cut(s) 397
XspI CTAG 5 cut(s) 540, 573, 1232, 1368, 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.