pycom03g12650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006259
Physical Location & Seq
Forward (+)
13387035 .. 13390156
3122 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
ATGTCGACGACGGAAGCTCTCTACTCCAAACTCTACGAAAAGTACACAAAAATCAAGACGAAGAAATGGTCGGAGTTGGATGAAATTGGTAGAGAACAAGAAGGAAAGTTCAAGCATTATGTATCTGTTGCAGAGGAGTATATACAACATTTGCGAAATCAAAATGAGAGGTTGCGCTTGGAAGTTGATGAGCTGAGAAGTGAAGTGGCTTCTATTAGGTCCACCAAAGATGAACAATGTACCGAGTACCAGAATCTTTTGATGGAAGAGAACAGGACAAATAAAGAACTTTCTGAAGAAGTAGAGAGGCTCCGAAAGCTGCAACAGGTGGGAAATCTATCGAATGATAGCAACACAGACAATGGTGGACTGTGTACACCTGCTAGTGGTCAAGTATCCGGAGCAGCAGGAAATCTCTCAAAGAGAAGAAGGACAAGTAAACGTAGGAAGAATTCCTACTCTGAGACAGAGGATATAGTTATGCCTTCTTGTGGGGCTGTTATAACTGTTCCAGAGGTACAATGGTGTAGAACGATTGAAAGACAAGGTTGTGTAAAACCAATTGGCCAAGTGAACTGCGTGTTTCAAGTGCTTGTTGAGTATATGGTGGATTTAAAGTTCTCGACTGTTAGTCAAGCCGAAGAAATATGCATCTCAGCTGTGCATCAATCAAGTGGTTACGCCTTCAATCTTACATGGATGAACAGAGCTGCAGGTAAAATAGAGCTTCTGTACCGCACCTCATCCTTGGGAACGCTTGAGAGGGTAGCACCAGAATGGATGAAGGATGACATAGTGTTAAGCCCAAACATGTGCCCTATCTTCTTTGAAAGGTTATCTCGTGTTGTCAAGCTGCACTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

32.88

Weight (kDa)

6.91

Isoelectric Point (pI)

54.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7806 PF25091 191 - 284 3.9e-42 Domain of unknown function (DUF7806)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 503
AarI CACCTGC 1 cut(s) 388
Acc36I ACCTGC 2 cut(s) 388, 704
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 1 cut(s) 736
AcoI YGGCCR 1 cut(s) 565
AcsI RAATTY 1 cut(s) 451
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 6 cut(s) 44, 241, 248, 376, 519, 734
AfiI CCNNNNNNNGG 2 cut(s) 386, 491
AflIII ACRYGT 1 cut(s) 810
AgsI TTSAA 5 cut(s) 112, 539, 587, 688, 830
AhdI GACNNNNNGTC 1 cut(s) 630
AloI GAACNNNNNNTCC 2 cut(s) 602, 634
AluBI AGCT 7 cut(s) 17, 193, 319, 659, 710, 727, 853
AluI AGCT 7 cut(s) 17, 193, 319, 659, 710, 727, 853
Alw26I GTCTC 1 cut(s) 458
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 1 cut(s) 565
ApeKI GCWGC 4 cut(s) 319, 404, 710, 853
ApoI RAATTY 1 cut(s) 451
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 1 cut(s) 219
AvaII GGWCC 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 818
BaeI ACNNNNGTAYC 2 cut(s) 509, 542
BalI TGGCCA 1 cut(s) 567
BarI GAAGNNNNNNTAC 2 cut(s) 440, 472
BauI CACGAG 1 cut(s) 840
BbvI GCAGC 4 cut(s) 306, 416, 697, 840
BccI CCATC 1 cut(s) 256
BciVI GTATCC 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 2 cut(s) 384, 862
BfmI CTRYAG 1 cut(s) 711
BfuAI ACCTGC 2 cut(s) 388, 704
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 4 cut(s) 320, 405, 711, 854
BlsI GCNGC 4 cut(s) 321, 406, 712, 855
Bme18I GGWCC 1 cut(s) 219
BmeRI GACNNNNNGTC 1 cut(s) 630
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 1 cut(s) 311
BmsI GCATC 2 cut(s) 660, 673
BpuEI CTTGAG 1 cut(s) 779
BsaJI CCNNGG 1 cut(s) 747
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 2 cut(s) 386, 491
BseAI TCCGGA 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 747
BseGI GGATG 5 cut(s) 85, 705, 743, 786, 793
BseLI CCNNNNNNNGG 2 cut(s) 386, 491
BseMII CTCAG 3 cut(s) 185, 453, 669
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 818
BseXI GCAGC 4 cut(s) 306, 416, 697, 840
BsgI GTGCAG 1 cut(s) 839
BshFI GGCC 1 cut(s) 567
BsiSI CCGG 1 cut(s) 399
BslI CCNNNNNNNGG 2 cut(s) 386, 491
BsmAI GTCTC 1 cut(s) 458
BsnI GGCC 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 818
Bsp13I TCCGGA 1 cut(s) 398
Bsp1407I TGTACA 1 cut(s) 374
BspACI CCGC 1 cut(s) 736
BspANI GGCC 1 cut(s) 567
BspCNI CTCAG 3 cut(s) 186, 454, 668
BspEI TCCGGA 1 cut(s) 398
BspLI GGNNCC 1 cut(s) 311
BspMAI CTGCAG 1 cut(s) 715
BspMI ACCTGC 2 cut(s) 388, 704
BsrGI TGTACA 1 cut(s) 374
BssECI CCNNGG 1 cut(s) 747
BssSI CACGAG 1 cut(s) 840
BssT1I CCWWGG 1 cut(s) 747
Bst2BI CACGAG 1 cut(s) 840
Bst4CI ACNGT 3 cut(s) 372, 508, 628
Bst6I CTCTTC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 374
BstDEI CTNAG 3 cut(s) 194, 462, 655
BstF5I GGATG 5 cut(s) 85, 705, 743, 786, 793
BstHHI GCGC 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 458
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstNSI RCATGY 1 cut(s) 814
BstSFI CTRYAG 1 cut(s) 711
BstSLI GKGCMC 1 cut(s) 818
BstV1I GCAGC 4 cut(s) 306, 416, 697, 840
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 1 cut(s) 567
BtsCI GGATG 5 cut(s) 85, 705, 743, 786, 793
BveI ACCTGC 2 cut(s) 388, 704
CfoI GCGC 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 219
Csp6I GTAC 6 cut(s) 43, 240, 247, 375, 518, 733
CviAII CATG 2 cut(s) 696, 811
CviQI GTAC 6 cut(s) 43, 240, 247, 375, 518, 733
DdeI CTNAG 3 cut(s) 194, 462, 655
DraI TTTAAA 1 cut(s) 615
DriI GACNNNNNGTC 1 cut(s) 630
EaeI YGGCCR 1 cut(s) 565
Eam1104I CTCTTC 1 cut(s) 261
Eam1105I GACNNNNNGTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 747
Eco47I GGWCC 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 315
EcoRI GAATTC 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 747
EcoT22I ATGCAT 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 747
FaeI CATG 2 cut(s) 699, 814
FatI CATG 2 cut(s) 695, 810
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 320, 405, 711, 854
FokI GGATG 5 cut(s) 92, 712, 730, 793, 800
Fsp4HI GCNGC 4 cut(s) 320, 405, 711, 854
FspBI CTAG 2 cut(s) 384, 862
GlaI GCGC 1 cut(s) 176
GluI GCNGC 4 cut(s) 320, 405, 711, 854
HaeIII GGCC 1 cut(s) 567
HapII CCGG 1 cut(s) 399
HhaI GCGC 1 cut(s) 177
Hin1II CATG 2 cut(s) 699, 814
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 253
HpaII CCGG 1 cut(s) 399
Hpy166II GTNNAC 8 cut(s) 6, 45, 222, 368, 375, 377, 440, 574
Hpy188I TCNGA 4 cut(s) 73, 295, 314, 463
Hpy188III TCNNGA 4 cut(s) 55, 399, 512, 622
Hpy8I GTNNAC 8 cut(s) 6, 45, 222, 368, 375, 377, 440, 574
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 5 cut(s) 95, 423, 495, 694, 778
HpyCH4III ACNGT 3 cut(s) 372, 508, 628
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 6 cut(s) 131, 322, 651, 664, 713, 856
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 3 cut(s) 194, 462, 655
HpySE526I ACGT 1 cut(s) 442
Hsp92II CATG 2 cut(s) 699, 814
HspAI GCGC 1 cut(s) 175
Kpn2I TCCGGA 1 cut(s) 398
LmnI GCTCC 2 cut(s) 315, 401
LpnPI CCDG 9 cut(s) 259, 263, 311, 393, 393, 412, 525, 699, 786
Lsp1109I GCAGC 4 cut(s) 306, 416, 697, 840
LweI GCATC 2 cut(s) 660, 673
MaeI CTAG 2 cut(s) 384, 862
MaeII ACGT 1 cut(s) 442
MaeIII GTNAC 1 cut(s) 677
MboII GAAGA 7 cut(s) 73, 278, 308, 438, 460, 653, 814
MfeI CAATTG 1 cut(s) 561
MhlI GDGCHC 1 cut(s) 818
MlsI TGGCCA 1 cut(s) 567
MluCI AATT 3 cut(s) 84, 451, 561
MluNI TGGCCA 1 cut(s) 567
MmeI TCCRAC 2 cut(s) 51, 57
MnlI CCTC 7 cut(s) 127, 162, 300, 463, 508, 751, 756
Mox20I TGGCCA 1 cut(s) 567
Mph1103I ATGCAT 1 cut(s) 653
MroI TCCGGA 1 cut(s) 398
MscI TGGCCA 1 cut(s) 567
MseI TTAA 2 cut(s) 614, 800
MslI CAYNNNNRTG 1 cut(s) 775
Msp20I TGGCCA 1 cut(s) 567
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 1 cut(s) 399
MunI CAATTG 1 cut(s) 561
MwoI GCNNNNNNNGC 1 cut(s) 316
NlaIII CATG 2 cut(s) 699, 814
NlaIV GGNNCC 1 cut(s) 311
NsiI ATGCAT 1 cut(s) 653
NspI RCATGY 1 cut(s) 814
PaqCI CACCTGC 1 cut(s) 388
PciI ACATGT 1 cut(s) 810
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 4 cut(s) 321, 406, 712, 855
PscI ACATGT 1 cut(s) 810
PsiI TTATAA 1 cut(s) 503
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 1 cut(s) 219
PstI CTGCAG 1 cut(s) 715
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 6 cut(s) 44, 241, 248, 376, 519, 734
RsaNI GTAC 6 cut(s) 43, 240, 247, 375, 518, 733
RseI CAYNNNNRTG 1 cut(s) 775
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 614, 800
SatI GCNGC 4 cut(s) 320, 405, 711, 854
Sau96I GGNCC 1 cut(s) 219
SduI GDGCHC 1 cut(s) 818
SfaNI GCATC 2 cut(s) 660, 673
SfcI CTRYAG 1 cut(s) 711
SinI GGWCC 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 775
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
Sse9I AATT 3 cut(s) 84, 451, 561
SsiI CCGC 1 cut(s) 736
SspMI CTAG 2 cut(s) 384, 862
StyI CCWWGG 1 cut(s) 747
TaaI ACNGT 3 cut(s) 372, 508, 628
TaiI ACGT 1 cut(s) 445
TaqI TCGA 3 cut(s) 5, 341, 623
TasI AATT 3 cut(s) 84, 451, 561
TatI WGTACW 2 cut(s) 42, 374
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 2 cut(s) 614, 800
Tru9I TTAA 2 cut(s) 614, 800
TseI GCWGC 4 cut(s) 319, 404, 710, 853
TspDTI ATGAA 4 cut(s) 96, 246, 716, 797
TspGWI ACGGA 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 219
XapI RAATTY 1 cut(s) 451
XceI RCATGY 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 519
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 384, 862
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.