pycom03g15040

5'-adenylylsulfate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007763
Physical Location & Seq
Reverse (-)
16412130 .. 16414175
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1200 bp
ATGAACCGTGACACTCGTCAACTGTTTCCCGTACCCCATCTTAATGCTTTTCCTGTGCCCTCCACGCCAACACATTACAGACCCTCTCTCTCTCTCTCTCTAAGCATTCAAATTTCTCTCATGGCCTTCGTAGCTACGTCTCCTTCCACCCAATCATTGCTTCACGCTTCGCTATCTTCCCACGAGCCCAAAGCTCAATGGACATCTTCTGTGAATCCGTCGGATCAATTTTCGTTTGCATCGACGGCTGTGGCCTCTTTCCAGAGACCATCATTGGCAAAACCCCTTTCCGCAGAATCGAAGAGGAGCAAATCAGTTGTTCCGCTTGCTGCAAAAATGGCATCTCCAGATGTTGACAACAATGTGGTAGGAGAAGATTACGAAAAACTGGCAAAAGAGTTGGAAAACGCTTCCCCACTTCAAATCATGAAGAAGGCTCTTGACAAATTTGGGAATGACATTGCCATTGCTTTCAGCGGAGCTGAGGACGTCGCCTTGATCGAGTATGCCAAACTAACAGGTCGGCCCTTCAGGGTATTCAGCCTGGATACTGGGAGGCTTAACCCAGAGACGTACCAGTTTTTTGACACTGTTGAGAAGCACTATGGCATTCACATTGAATACATGTTTCCTGATGCTGTTGAAGTTCAAGCACTGGTGCGAGGCAAAGGACTGTTCTCATTCTATGAAGACGGCCACCAGGAGTGCTGCAGGGTAAGGAAGGTTAGGCCTTTAAGGCGAGCCCTTAAGGGTCTTCGTGCCTGGATCACTGGGCAGAGAAAAGACCAGTCTCCTGGCACAAGAGCTGAAATTCCGGTTGTCCAGGTGGACCCATCGTTTGAAGGAATTGATGGTGGAATTGGCAGCTTGGTGAAATGGAATCCTGTTGCCAACGTGGAAGGCCATGACATATGGAACTTCCTCCAAGTCATGAATGTACCTGTCAATCCTTTGCACTCGCAAGGATACATTTCAATCGGGTGCGAGCCATGCACAAGGCCGGTGCTGCCAGGGCAGCATGAAAGGGAGGGAAGATGGTGGTGGGAGGATGCTAAGGCAAAGGAATGTGGCCTCCATAACGGAAATATCAAAGAAAAAGAGGCGCTGCACCTCAATGGCAATGGGAATGGGATCGATCAACAACAAATGGGAGTGCCAATGTCCCAGACATCTTTAACAGCAAGAACTTGGTTTCCTTGA

Protein Analysis

400

Amino Acids

44.29

Weight (kDa)

7.2

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAPS_reduct PF01507 154 - 334 2.4e-49 Phosphoadenosine phosphosulfate reductase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 492
AciI CCGC 3 cut(s) 291, 323, 477
AclWI GGATC 3 cut(s) 231, 773, 1139
AcoI YGGCCR 1 cut(s) 694
AcsI RAATTY 3 cut(s) 111, 446, 810
AcuI CTGAAG 1 cut(s) 514
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 3 cut(s) 33, 575, 939
AflII CTTAAG 1 cut(s) 746
AflIII ACRYGT 1 cut(s) 624
AgsI TTSAA 7 cut(s) 110, 422, 620, 644, 650, 842, 975
AjnI CCWGG 6 cut(s) 543, 699, 761, 793, 822, 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 144, 176
AloI GAACNNNNNNTCC 1 cut(s) 1177
AluBI AGCT 5 cut(s) 134, 194, 482, 806, 867
AluI AGCT 5 cut(s) 134, 194, 482, 806, 867
Alw26I GTCTC 4 cut(s) 144, 259, 563, 795
AlwI GGATC 3 cut(s) 231, 773, 1139
AoxI GGCC 8 cut(s) 123, 252, 524, 694, 728, 901, 998, 1069
ApeKI GCWGC 6 cut(s) 329, 708, 864, 1006, 1015, 1105
ApoI RAATTY 3 cut(s) 111, 446, 810
ArsI GACNNNNNNTTYG 2 cut(s) 821, 853
AspLEI GCGC 1 cut(s) 1105
AspS9I GGNCC 2 cut(s) 525, 829
AsuHPI GGTGA 1 cut(s) 883
AvaII GGWCC 1 cut(s) 829
BaeGI GKGCMC 1 cut(s) 60
BanII GRGCYC 2 cut(s) 189, 745
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 2 cut(s) 696, 746
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 6 cut(s) 316, 695, 876, 993, 1027, 1092
BccI CCATC 5 cut(s) 45, 277, 841, 845, 1029
BceAI ACGGC 2 cut(s) 261, 709
BciT130I CCWGG 6 cut(s) 545, 701, 763, 795, 824, 1011
BciVI GTATCC 2 cut(s) 541, 959
BcoDI GTCTC 4 cut(s) 144, 259, 563, 795
BfmI CTRYAG 1 cut(s) 709
BfoI RGCGCY 1 cut(s) 1106
BfrI CTTAAG 1 cut(s) 746
BfuI GTATCC 2 cut(s) 541, 959
BglI GCCNNNNNGGC 1 cut(s) 736
BisI GCNGC 6 cut(s) 330, 709, 865, 1007, 1016, 1106
BlsI GCNGC 6 cut(s) 331, 710, 866, 1008, 1017, 1107
Bme1390I CCNGG 6 cut(s) 545, 701, 763, 795, 824, 1011
Bme18I GGWCC 1 cut(s) 829
BmgT120I GGNCC 2 cut(s) 525, 829
BmiI GGNNCC 1 cut(s) 831
BmrFI CCNGG 6 cut(s) 545, 701, 763, 795, 824, 1011
BmrI ACTGGG 2 cut(s) 561, 780
BmsI GCATC 4 cut(s) 248, 350, 625, 1039
BmuI ACTGGG 2 cut(s) 561, 780
BoxI GACNNNNGTC 1 cut(s) 15
BpiI GAAGAC 2 cut(s) 696, 746
BpmI CTGGAG 1 cut(s) 330
Bpu10I CCTNAGC 2 cut(s) 483, 1053
Bsa29I ATCGAT 1 cut(s) 1134
BsaHI GRCGYC 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 1010
BsaWI WCCGGW 1 cut(s) 814
Bse118I RCCGGY 1 cut(s) 1000
Bse1I ACTGG 6 cut(s) 393, 556, 577, 660, 775, 787
Bse3DI GCAATG 4 cut(s) 155, 459, 465, 1126
BseBI CCWGG 6 cut(s) 545, 701, 763, 795, 824, 1011
BseCI ATCGAT 1 cut(s) 1134
BseDI CCNNGG 1 cut(s) 1010
BseGI GGATG 1 cut(s) 1054
BseMI GCAATG 4 cut(s) 155, 459, 465, 1126
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 6 cut(s) 393, 556, 577, 660, 775, 787
BseRI GAGGAG 1 cut(s) 319
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 6 cut(s) 316, 695, 876, 993, 1027, 1092
BsgI GTGCAG 1 cut(s) 1091
BshFI GGCC 8 cut(s) 125, 254, 526, 696, 730, 903, 1000, 1071
BshVI ATCGAT 1 cut(s) 1134
BsiSI CCGG 2 cut(s) 815, 1001
BslFI GGGAC 1 cut(s) 1147
BsmAI GTCTC 4 cut(s) 144, 259, 563, 795
BsmBI CGTCTC 2 cut(s) 144, 563
BsmFI GGGAC 1 cut(s) 1147
BsmI GAATGC 2 cut(s) 105, 609
BsnI GGCC 8 cut(s) 125, 254, 526, 696, 730, 903, 1000, 1071
Bso31I GGTCTC 1 cut(s) 259
Bsp1286I GDGCHC 3 cut(s) 60, 189, 745
Bsp143I GATC 5 cut(s) 223, 498, 765, 1131, 1135
BspACI CCGC 3 cut(s) 291, 323, 477
BspANI GGCC 8 cut(s) 125, 254, 526, 696, 730, 903, 1000, 1071
BspCNI CTCAG 1 cut(s) 475
BspDI ATCGAT 1 cut(s) 1134
BspHI TCATGA 2 cut(s) 426, 930
BspLI GGNNCC 1 cut(s) 831
BspMAI CTGCAG 1 cut(s) 713
BspPI GGATC 3 cut(s) 231, 773, 1139
BspTI CTTAAG 1 cut(s) 746
BspTNI GGTCTC 1 cut(s) 259
BsrDI GCAATG 4 cut(s) 155, 459, 465, 1126
BsrFI RCCGGY 1 cut(s) 1000
BsrI ACTGG 6 cut(s) 393, 556, 577, 660, 775, 787
BssAI RCCGGY 1 cut(s) 1000
BssECI CCNNGG 1 cut(s) 1010
BssMI GATC 5 cut(s) 223, 498, 765, 1131, 1135
BssNI GRCGYC 1 cut(s) 489
BssSI CACGAG 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 6 cut(s) 545, 701, 763, 795, 824, 1011
Bst4CI ACNGT 4 cut(s) 8, 24, 592, 675
Bst6I CTCTTC 1 cut(s) 296
BstACI GRCGYC 1 cut(s) 489
BstAFI CTTAAG 1 cut(s) 746
BstC8I GCNNGC 3 cut(s) 327, 741, 986
BstDEI CTNAG 3 cut(s) 101, 483, 1053
BstF5I GGATG 1 cut(s) 1054
BstH2I RGCGCY 1 cut(s) 1106
BstHHI GCGC 1 cut(s) 1105
BstKTI GATC 5 cut(s) 226, 501, 768, 1134, 1138
BstMAI GTCTC 4 cut(s) 144, 259, 563, 795
BstMBI GATC 5 cut(s) 223, 498, 765, 1131, 1135
BstMWI GCNNNNNNNGC 9 cut(s) 64, 131, 245, 338, 736, 990, 1006, 1012, 1015
BstNI CCWGG 6 cut(s) 545, 701, 763, 795, 824, 1011
BstNSI RCATGY 1 cut(s) 628
BstPAI GACNNNNGTC 1 cut(s) 15
BstSCI CCNGG 6 cut(s) 543, 699, 761, 793, 822, 1009
BstSFI CTRYAG 1 cut(s) 709
BstSLI GKGCMC 1 cut(s) 60
BstV1I GCAGC 6 cut(s) 316, 695, 876, 993, 1027, 1092
BstV2I GAAGAC 2 cut(s) 696, 746
BstXI CCANNNNNNTGG 1 cut(s) 794
Bsu15I ATCGAT 1 cut(s) 1134
BsuI GTATCC 2 cut(s) 541, 959
BsuRI GGCC 8 cut(s) 125, 254, 526, 696, 730, 903, 1000, 1071
BsuTUI ATCGAT 1 cut(s) 1134
BtsCI GGATG 1 cut(s) 1054
BtsIMutI CAGTG 3 cut(s) 588, 653, 768
Cac8I GCNNGC 3 cut(s) 327, 741, 986
CciI TCATGA 2 cut(s) 426, 930
CfoI GCGC 1 cut(s) 1105
Cfr10I RCCGGY 1 cut(s) 1000
Cfr13I GGNCC 2 cut(s) 525, 829
ClaI ATCGAT 1 cut(s) 1134
Csp6I GTAC 3 cut(s) 32, 574, 938
CviAII CATG 7 cut(s) 121, 427, 625, 905, 931, 990, 1019
CviQI GTAC 3 cut(s) 32, 574, 938
DdeI CTNAG 3 cut(s) 101, 483, 1053
DpnI GATC 5 cut(s) 225, 500, 767, 1133, 1137
DpnII GATC 5 cut(s) 223, 498, 765, 1131, 1135
EaeI YGGCCR 1 cut(s) 694
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco147I AGGCCT 1 cut(s) 730
Eco24I GRGCYC 2 cut(s) 189, 745
Eco31I GGTCTC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 829
Eco57I CTGAAG 1 cut(s) 514
EcoRII CCWGG 6 cut(s) 543, 699, 761, 793, 822, 1009
EcoT38I GRGCYC 2 cut(s) 189, 745
Esp3I CGTCTC 2 cut(s) 144, 563
FaeI CATG 7 cut(s) 124, 430, 628, 908, 934, 993, 1022
FaqI GGGAC 1 cut(s) 1147
FatI CATG 7 cut(s) 120, 426, 624, 904, 930, 989, 1018
FauNDI CATATG 1 cut(s) 911
Fnu4HI GCNGC 6 cut(s) 330, 709, 865, 1007, 1016, 1106
FokI GGATG 1 cut(s) 1061
FriOI GRGCYC 2 cut(s) 189, 745
Fsp4HI GCNGC 6 cut(s) 330, 709, 865, 1007, 1016, 1106
GlaI GCGC 1 cut(s) 1104
GluI GCNGC 6 cut(s) 330, 709, 865, 1007, 1016, 1106
GsuI CTGGAG 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 1106
HaeIII GGCC 8 cut(s) 125, 254, 526, 696, 730, 903, 1000, 1071
HapII CCGG 2 cut(s) 815, 1001
HhaI GCGC 1 cut(s) 1105
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 7 cut(s) 124, 430, 628, 908, 934, 993, 1022
Hin6I GCGC 1 cut(s) 1103
HinP1I GCGC 1 cut(s) 1103
HincII GTYRAC 2 cut(s) 20, 355
HindII GTYRAC 2 cut(s) 20, 355
HinfI GANTC 3 cut(s) 214, 296, 880
HpaII CCGG 2 cut(s) 815, 1001
HphI GGTGA 1 cut(s) 883
Hpy166II GTNNAC 3 cut(s) 20, 355, 829
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 6 cut(s) 262, 347, 427, 440, 632, 931
Hpy8I GTNNAC 3 cut(s) 20, 355, 829
Hpy99I CGWCG 3 cut(s) 223, 247, 494
HpyAV CCTTC 7 cut(s) 136, 153, 427, 538, 715, 836, 893
HpyCH4III ACNGT 4 cut(s) 8, 24, 592, 675
HpyCH4IV ACGT 4 cut(s) 137, 489, 572, 894
HpyCH4V TGCA 6 cut(s) 239, 332, 711, 955, 993, 1108
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 131, 245, 338, 736, 990, 1006, 1012, 1015
HpyF3I CTNAG 3 cut(s) 101, 483, 1053
HpySE526I ACGT 4 cut(s) 137, 489, 572, 894
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 7 cut(s) 124, 430, 628, 908, 934, 993, 1022
HspAI GCGC 1 cut(s) 1103
Kzo9I GATC 5 cut(s) 223, 498, 765, 1131, 1135
LmnI GCTCC 2 cut(s) 306, 479
Lsp1109I GCAGC 6 cut(s) 316, 695, 876, 993, 1027, 1092
LweI GCATC 4 cut(s) 248, 350, 625, 1039
MaeII ACGT 4 cut(s) 137, 489, 572, 894
MaeIII GTNAC 1 cut(s) 8
MalI GATC 5 cut(s) 225, 500, 767, 1133, 1137
MboI GATC 5 cut(s) 223, 498, 765, 1131, 1135
MboII GAAGA 8 cut(s) 168, 198, 313, 386, 442, 701, 746, 1044
MhlI GDGCHC 3 cut(s) 60, 189, 745
MluCI AATT 6 cut(s) 111, 227, 446, 810, 846, 858
MmeI TCCRAC 2 cut(s) 201, 381
MseI TTAA 5 cut(s) 42, 561, 734, 747, 1175
MslI CAYNNNNRTG 2 cut(s) 42, 1113
MspA1I CMGCKG 1 cut(s) 477
MspCI CTTAAG 1 cut(s) 746
MspI CCGG 2 cut(s) 815, 1001
MspR9I CCNGG 6 cut(s) 545, 701, 763, 795, 824, 1011
Mva1269I GAATGC 2 cut(s) 105, 609
MvaI CCWGG 6 cut(s) 545, 701, 763, 795, 824, 1011
MwoI GCNNNNNNNGC 9 cut(s) 64, 131, 245, 338, 736, 990, 1006, 1012, 1015
NdeI CATATG 1 cut(s) 911
NdeII GATC 5 cut(s) 223, 498, 765, 1131, 1135
NlaIII CATG 7 cut(s) 124, 430, 628, 908, 934, 993, 1022
NlaIV GGNNCC 1 cut(s) 831
NmuCI GTSAC 1 cut(s) 8
NspI RCATGY 1 cut(s) 628
PagI TCATGA 2 cut(s) 426, 930
PceI AGGCCT 1 cut(s) 730
PciI ACATGT 1 cut(s) 624
PcsI WCGNNNNNNNCGW 2 cut(s) 239, 498
PctI GAATGC 2 cut(s) 105, 609
PfeI GAWTC 3 cut(s) 214, 296, 880
PkrI GCNGC 6 cut(s) 331, 710, 866, 1008, 1017, 1107
PscI ACATGT 1 cut(s) 624
PshAI GACNNNNGTC 1 cut(s) 15
Psp6I CCWGG 6 cut(s) 543, 699, 761, 793, 822, 1009
PspGI CCWGG 6 cut(s) 543, 699, 761, 793, 822, 1009
PspN4I GGNNCC 1 cut(s) 831
PspPI GGNCC 2 cut(s) 525, 829
PstI CTGCAG 1 cut(s) 713
RsaI GTAC 3 cut(s) 33, 575, 939
RsaNI GTAC 3 cut(s) 32, 574, 938
RseI CAYNNNNRTG 2 cut(s) 42, 1113
SaqAI TTAA 5 cut(s) 42, 561, 734, 747, 1175
SatI GCNGC 6 cut(s) 330, 709, 865, 1007, 1016, 1106
Sau3AI GATC 5 cut(s) 223, 498, 765, 1131, 1135
Sau96I GGNCC 2 cut(s) 525, 829
ScrFI CCNGG 6 cut(s) 545, 701, 763, 795, 824, 1011
SduI GDGCHC 3 cut(s) 60, 189, 745
SfaNI GCATC 4 cut(s) 248, 350, 625, 1039
SfcI CTRYAG 1 cut(s) 709
SinI GGWCC 1 cut(s) 829
SmiMI CAYNNNNRTG 2 cut(s) 42, 1113
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
Sse9I AATT 6 cut(s) 111, 227, 446, 810, 846, 858
SseBI AGGCCT 1 cut(s) 730
SsiI CCGC 3 cut(s) 291, 323, 477
StuI AGGCCT 1 cut(s) 730
StyD4I CCNGG 6 cut(s) 543, 699, 761, 793, 822, 1009
TaaI ACNGT 4 cut(s) 8, 24, 592, 675
TaiI ACGT 4 cut(s) 140, 492, 575, 897
TaqI TCGA 4 cut(s) 242, 299, 501, 1134
TasI AATT 6 cut(s) 111, 227, 446, 810, 846, 858
TfiI GAWTC 3 cut(s) 214, 296, 880
Tru1I TTAA 5 cut(s) 42, 561, 734, 747, 1175
Tru9I TTAA 5 cut(s) 42, 561, 734, 747, 1175
TscAI CASTG 3 cut(s) 595, 660, 775
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 6 cut(s) 329, 708, 864, 1006, 1015, 1105
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 5 cut(s) 17, 443, 702, 947, 1035
TspGWI ACGGA 2 cut(s) 207, 1095
TspRI CASTG 3 cut(s) 595, 660, 775
Vha464I CTTAAG 1 cut(s) 746
VpaK11BI GGWCC 1 cut(s) 829
XapI RAATTY 3 cut(s) 111, 446, 810
XceI RCATGY 1 cut(s) 628
ZraI GACGTC 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.