pycom03g19360

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0004482
Physical Location & Seq
Reverse (-)
19964915 .. 19966005
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGGTTGATCGGTCTGAGCCATTTGACAAGAAAAATGAGGAGCTCCAGGAAAGAGTCGCCTTGGAGGCTCATCTAGCGAAACTGTTTGCAAGCGTTTCATCTGTTAAAGCTGCGTATGCACAACTGCAGCATTCTCAGTCTCCCTATGATGCTGAAGGAATCCAAGCTGCTGACAAAGAGGTAGTCTCCGAATTGAAGAACTTGTCTGAGTTGAAGCGTTGTTTCTTAAAAAAACAGTTCGATCGATCTAAACCTGAGACTACCCTGGTTTTGGCGGAGATCGAGGAGCAGAAGAGTGTTCTAAAAACCTATGAAATCATGGGGAAAAAGTTGGAATCTCAGGTGAGGCTGAAGGACTCTGAAATTGTATTTCTCGAAGAAAAGTTAGAGGAGGCCAAGGATCATAATAAGTTGCTTGAGAAGCGATTAAACCAGAACCAGAGCGGGCAGCTACATGTGCTCGACAATCTTCATTTATCTGCTTTAAGTCCTAGCCATTTCATTACAGTTCTTCGACACACGGTAAAGTCCATCCGGAGCTTTGTTCGGATGATGGTGGATGATATGAAATCTGCCGGTTGGGACATTCATGCAGCAGCTAAAGCGATTGACCGCGATGTGCTTTATTGGAAAGAAGATCACAAGTGTTTTGCATTTGAACACTCTGTTTGCAAGGAAATGTTTGATGCTTTCCAGTACCCCAACTTCTCACTTCCAAATGAGTCCTTGCCGGAGAAAAAGAAACAACAGCAGCAGCTCTTTTTTGTGAGATTCACGGAACTCAAATCGATGAAGCCGAAGGACTATCTCTCCCAGAATCCCAGATCAGCATTTGCCAAGTTCTGCCGTGTCAAGTACTTACGACTGGTTCATCCCAAGATGGAAACATCATTTTTCGGCAATCTGAATCAAAGAAACCTCATCAATGCAGGTGAGTTTCCGAGCTCTGATTTCTTTGCTTCATTTGCTGAAATGGCAAAGCGTGTCTGGCTCCTGCATTGCTTGGCCTTCTCCTTCGATCCTGAAGCCGCAATCTTCCAACTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

350

Amino Acids

40.54

Weight (kDa)

8.41

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF641 PF04859 18 - 142 2.4e-45 Plant protein of unknown function (DUF641)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 920
Acc36I ACCTGC 1 cut(s) 920
AccBSI CCGCTC 1 cut(s) 444
AccII CGCG 1 cut(s) 615
AccIII TCCGGA 1 cut(s) 534
AciI CCGC 4 cut(s) 275, 444, 613, 1029
AclWI GGATC 2 cut(s) 408, 1013
AcuI CTGAAG 3 cut(s) 174, 371, 1044
AfaI GTAC 2 cut(s) 698, 857
AfiI CCNNNNNNNGG 1 cut(s) 271
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 3 cut(s) 196, 214, 659
AjnI CCWGG 2 cut(s) 45, 264
AluBI AGCT 8 cut(s) 43, 110, 167, 451, 540, 599, 757, 945
AluI AGCT 8 cut(s) 43, 110, 167, 451, 540, 599, 757, 945
Alw21I GWGCWC 3 cut(s) 45, 462, 947
Alw26I GTCTC 3 cut(s) 144, 190, 251
AlwI GGATC 2 cut(s) 408, 1013
Aor13HI TCCGGA 1 cut(s) 534
AoxI GGCC 2 cut(s) 393, 1005
ApeKI GCWGC 8 cut(s) 110, 127, 167, 448, 593, 596, 751, 754
ArsI GACNNNNNNTTYG 2 cut(s) 168, 200
AsuHPI GGTGA 2 cut(s) 355, 944
BanII GRGCYC 2 cut(s) 45, 947
BarI GAAGNNNNNNTAC 2 cut(s) 689, 721
Bbv12I GWGCWC 3 cut(s) 45, 462, 947
BbvI GCAGC 8 cut(s) 97, 139, 154, 460, 605, 608, 763, 766
BccI CCATC 3 cut(s) 539, 547, 874
BceAI ACGGC 1 cut(s) 831
BciT130I CCWGG 2 cut(s) 47, 266
BcoDI GTCTC 3 cut(s) 144, 190, 251
BfaI CTAG 2 cut(s) 74, 492
BfmI CTRYAG 1 cut(s) 125
BfuAI ACCTGC 1 cut(s) 920
BglI GCCNNNNNGGC 1 cut(s) 65
BisI GCNGC 9 cut(s) 111, 128, 168, 449, 594, 597, 752, 755, 1029
BlsI GCNGC 9 cut(s) 112, 129, 169, 450, 595, 598, 753, 756, 1030
BmcAI AGTACT 1 cut(s) 857
Bme1390I CCNGG 2 cut(s) 47, 266
BmiI GGNNCC 1 cut(s) 992
BmrFI CCNGG 2 cut(s) 47, 266
BmsI GCATC 2 cut(s) 139, 676
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpmI CTGGAG 1 cut(s) 29
BpuEI CTTGAG 1 cut(s) 437
Bsa29I ATCGAT 2 cut(s) 244, 788
BsaJI CCNNGG 3 cut(s) 60, 264, 396
BsaWI WCCGGW 1 cut(s) 534
BsaXI ACNNNNNCTCC 2 cut(s) 794, 824
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse118I RCCGGY 1 cut(s) 575
Bse1I ACTGG 2 cut(s) 694, 870
Bse3DI GCAATG 1 cut(s) 997
BseAI TCCGGA 1 cut(s) 534
BseBI CCWGG 2 cut(s) 47, 266
BseCI ATCGAT 2 cut(s) 244, 788
BseDI CCNNGG 3 cut(s) 60, 264, 396
BseGI GGATG 4 cut(s) 531, 555, 565, 871
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 997
BseMII CTCAG 5 cut(s) 6, 149, 198, 246, 353
BseNI ACTGG 2 cut(s) 694, 870
BseRI GAGGAG 3 cut(s) 53, 299, 404
BseXI GCAGC 8 cut(s) 97, 139, 154, 460, 605, 608, 763, 766
Bsh1236I CGCG 1 cut(s) 615
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 2 cut(s) 395, 1007
BshVI ATCGAT 2 cut(s) 244, 788
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 3 cut(s) 45, 462, 947
BsiSI CCGG 3 cut(s) 535, 576, 731
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 271
BsmAI GTCTC 3 cut(s) 144, 190, 251
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 2 cut(s) 395, 1007
Bsp1286I GDGCHC 3 cut(s) 45, 462, 947
Bsp13I TCCGGA 1 cut(s) 534
Bsp143I GATC 8 cut(s) 7, 241, 245, 279, 400, 637, 824, 1018
BspACI CCGC 4 cut(s) 275, 444, 613, 1029
BspANI GGCC 2 cut(s) 395, 1007
BspCNI CTCAG 5 cut(s) 7, 148, 199, 247, 352
BspDI ATCGAT 2 cut(s) 244, 788
BspEI TCCGGA 1 cut(s) 534
BspFNI CGCG 1 cut(s) 615
BspLI GGNNCC 1 cut(s) 992
BspMAI CTGCAG 1 cut(s) 129
BspMI ACCTGC 1 cut(s) 920
BspPI GGATC 2 cut(s) 408, 1013
BsrBI CCGCTC 1 cut(s) 444
BsrDI GCAATG 1 cut(s) 997
BsrFI RCCGGY 1 cut(s) 575
BsrI ACTGG 2 cut(s) 694, 870
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 3 cut(s) 60, 264, 396
BssMI GATC 8 cut(s) 7, 241, 245, 279, 400, 637, 824, 1018
BssT1I CCWWGG 2 cut(s) 60, 396
Bst2UI CCWGG 2 cut(s) 47, 266
Bst4CI ACNGT 4 cut(s) 84, 237, 508, 523
Bst6I CTCTTC 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 91, 446
BstDEI CTNAG 6 cut(s) 15, 135, 207, 255, 339, 1043
BstF5I GGATG 4 cut(s) 531, 555, 565, 871
BstFNI CGCG 1 cut(s) 615
BstKTI GATC 8 cut(s) 10, 244, 248, 282, 403, 640, 827, 1021
BstMAI GTCTC 3 cut(s) 144, 190, 251
BstMBI GATC 8 cut(s) 7, 241, 245, 279, 400, 637, 824, 1018
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 9 cut(s) 65, 74, 116, 421, 457, 602, 965, 974, 988
BstNI CCWGG 2 cut(s) 47, 266
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 45, 264
BstSFI CTRYAG 1 cut(s) 125
BstUI CGCG 1 cut(s) 615
BstV1I GCAGC 8 cut(s) 97, 139, 154, 460, 605, 608, 763, 766
Bsu15I ATCGAT 2 cut(s) 244, 788
BsuRI GGCC 2 cut(s) 395, 1007
BsuTUI ATCGAT 2 cut(s) 244, 788
BtgZI GCGATG 1 cut(s) 630
BtsCI GGATG 4 cut(s) 531, 555, 565, 871
BveI ACCTGC 1 cut(s) 920
Cac8I GCNNGC 2 cut(s) 91, 446
Cfr10I RCCGGY 1 cut(s) 575
ClaI ATCGAT 2 cut(s) 244, 788
Csp6I GTAC 2 cut(s) 697, 856
CviAII CATG 3 cut(s) 319, 455, 590
CviQI GTAC 2 cut(s) 697, 856
DdeI CTNAG 6 cut(s) 15, 135, 207, 255, 339, 1043
DpnI GATC 8 cut(s) 9, 243, 247, 281, 402, 639, 826, 1020
DpnII GATC 8 cut(s) 7, 241, 245, 279, 400, 637, 824, 1018
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
EciI GGCGGA 1 cut(s) 290
Ecl136II GAGCTC 2 cut(s) 43, 945
Eco130I CCWWGG 2 cut(s) 60, 396
Eco24I GRGCYC 2 cut(s) 45, 947
Eco53kI GAGCTC 2 cut(s) 43, 945
Eco57I CTGAAG 3 cut(s) 174, 371, 1044
EcoICRI GAGCTC 2 cut(s) 43, 945
EcoRII CCWGG 2 cut(s) 45, 264
EcoT14I CCWWGG 2 cut(s) 60, 396
EcoT38I GRGCYC 2 cut(s) 45, 947
ErhI CCWWGG 2 cut(s) 60, 396
FaeI CATG 3 cut(s) 322, 458, 593
FaiI YATR 8 cut(s) 117, 147, 312, 320, 405, 456, 566, 591
FaqI GGGAC 1 cut(s) 596
FatI CATG 3 cut(s) 318, 454, 589
FauI CCCGC 1 cut(s) 437
Fnu4HI GCNGC 9 cut(s) 111, 128, 168, 449, 594, 597, 752, 755, 1029
FokI GGATG 4 cut(s) 518, 562, 572, 858
FriOI GRGCYC 2 cut(s) 45, 947
Fsp4HI GCNGC 9 cut(s) 111, 128, 168, 449, 594, 597, 752, 755, 1029
FspBI CTAG 2 cut(s) 74, 492
GluI GCNGC 9 cut(s) 111, 128, 168, 449, 594, 597, 752, 755, 1029
GsuI CTGGAG 1 cut(s) 29
HaeIII GGCC 2 cut(s) 395, 1007
HapII CCGG 3 cut(s) 535, 576, 731
Hin1II CATG 3 cut(s) 322, 458, 593
HinfI GANTC 8 cut(s) 54, 159, 335, 356, 722, 771, 817, 907
HpaII CCGG 3 cut(s) 535, 576, 731
HphI GGTGA 2 cut(s) 355, 944
Hpy188I TCNGA 8 cut(s) 16, 190, 208, 361, 549, 906, 942, 949
Hpy188III TCNNGA 3 cut(s) 374, 535, 1022
HpyAV CCTTC 5 cut(s) 149, 346, 793, 1018, 1024
HpyCH4III ACNGT 4 cut(s) 84, 237, 508, 523
HpyCH4V TGCA 8 cut(s) 89, 119, 127, 593, 653, 672, 929, 997
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 74, 116, 421, 457, 602, 965, 974, 988
HpyF3I CTNAG 6 cut(s) 15, 135, 207, 255, 339, 1043
Hsp92II CATG 3 cut(s) 322, 458, 593
Kpn2I TCCGGA 1 cut(s) 534
Kzo9I GATC 8 cut(s) 7, 241, 245, 279, 400, 637, 824, 1018
LmnI GCTCC 5 cut(s) 40, 48, 286, 537, 996
Lsp1109I GCAGC 8 cut(s) 97, 139, 154, 460, 605, 608, 763, 766
LweI GCATC 2 cut(s) 139, 676
MaeI CTAG 2 cut(s) 74, 492
MalI GATC 8 cut(s) 9, 243, 247, 281, 402, 639, 826, 1020
MbiI CCGCTC 1 cut(s) 444
MboI GATC 8 cut(s) 7, 241, 245, 279, 400, 637, 824, 1018
MboII GAAGA 7 cut(s) 208, 304, 389, 461, 503, 647, 1027
MhlI GDGCHC 3 cut(s) 45, 462, 947
MluCI AATT 2 cut(s) 191, 363
MlyI GAGTC 3 cut(s) 63, 350, 731
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 8 cut(s) 31, 58, 172, 277, 339, 382, 385, 929
MroI TCCGGA 1 cut(s) 534
MseI TTAA 4 cut(s) 105, 227, 428, 485
MspI CCGG 3 cut(s) 535, 576, 731
MspR9I CCNGG 2 cut(s) 47, 266
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 2 cut(s) 47, 266
MvnI CGCG 1 cut(s) 615
MwoI GCNNNNNNNGC 9 cut(s) 65, 74, 116, 421, 457, 602, 965, 974, 988
NdeII GATC 8 cut(s) 7, 241, 245, 279, 400, 637, 824, 1018
NlaIII CATG 3 cut(s) 322, 458, 593
NlaIV GGNNCC 1 cut(s) 992
NspI RCATGY 1 cut(s) 458
PaqCI CACCTGC 1 cut(s) 920
PciI ACATGT 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 794
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 5 cut(s) 159, 335, 771, 817, 907
PfoI TCCNGGA 1 cut(s) 45
PkrI GCNGC 9 cut(s) 112, 129, 169, 450, 595, 598, 753, 756, 1030
Ple19I CGATCG 1 cut(s) 244
PleI GAGTC 3 cut(s) 62, 350, 730
PpsI GAGTC 3 cut(s) 62, 350, 730
PscI ACATGT 1 cut(s) 454
Psp124BI GAGCTC 2 cut(s) 45, 947
Psp6I CCWGG 2 cut(s) 45, 264
PspGI CCWGG 2 cut(s) 45, 264
PspN4I GGNNCC 1 cut(s) 992
PstI CTGCAG 1 cut(s) 129
PvuI CGATCG 1 cut(s) 244
RsaI GTAC 2 cut(s) 698, 857
RsaNI GTAC 2 cut(s) 697, 856
SacI GAGCTC 2 cut(s) 45, 947
SaqAI TTAA 4 cut(s) 105, 227, 428, 485
SatI GCNGC 9 cut(s) 111, 128, 168, 449, 594, 597, 752, 755, 1029
Sau3AI GATC 8 cut(s) 7, 241, 245, 279, 400, 637, 824, 1018
ScaI AGTACT 1 cut(s) 857
SchI GAGTC 3 cut(s) 63, 350, 731
ScrFI CCNGG 2 cut(s) 47, 266
SduI GDGCHC 3 cut(s) 45, 462, 947
SfaNI GCATC 2 cut(s) 139, 676
SfcI CTRYAG 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 2 cut(s) 191, 363
SsiI CCGC 4 cut(s) 275, 444, 613, 1029
SspMI CTAG 2 cut(s) 74, 492
SstI GAGCTC 2 cut(s) 45, 947
StyD4I CCNGG 2 cut(s) 45, 264
StyI CCWWGG 2 cut(s) 60, 396
TaaI ACNGT 4 cut(s) 84, 237, 508, 523
TaqI TCGA 8 cut(s) 240, 244, 282, 375, 462, 514, 788, 1017
TasI AATT 2 cut(s) 191, 363
TatI WGTACW 1 cut(s) 855
TauI GCSGC 1 cut(s) 1031
TfiI GAWTC 5 cut(s) 159, 335, 771, 817, 907
Tru1I TTAA 4 cut(s) 105, 227, 428, 485
Tru9I TTAA 4 cut(s) 105, 227, 428, 485
TseI GCWGC 8 cut(s) 110, 127, 167, 448, 593, 596, 751, 754
TspDTI ATGAA 9 cut(s) 87, 327, 461, 490, 578, 581, 806, 860, 951
TspGWI ACGGA 1 cut(s) 791
XceI RCATGY 1 cut(s) 458
XspI CTAG 2 cut(s) 74, 492
ZrmI AGTACT 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.