pycom03g20810

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010707
Physical Location & Seq
Forward (+)
21232755 .. 21235921
3167 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1143 bp
ATGAAGCACGTGTCGGTGGGTTTAGTTTGTTTTTCCGGTTTCCCAACAAATACAATTACAAAAACCCACTCCCCACATTCAGATAGTGGTGGTTGCGTTGACGACGGGATGGGCAGCAGCGGCAGCGCCGAAACGTCGTCGTTAATGAGGGACTTACTCAGGCAGGCTGGCGGAGTCGCGGTGGTCGACGGTGGACTTTCCACCGAGCTCGAACGCCACGGCGCTGACCTCAACGATCCTCTCTGGAGCGCCAAATGCCTCCTCACTTCCCCTCACCTCATTCGCAAGGTACACCTTGATTACCTTGAAGCTGGTGCAGATATCATAATCACAGCATCGTATCAGGCCACCATTCAAGGTTTCAAGGCCAAAGGCTTTTCTACAGAAGAGAGTGAATCTTTGCTTAGGAAAAGTGTTGAAATTGCCCAAGAGGCACGAGATATCTATTACGACAGATGTACTCAATGCTCTTCTGCTGACAGTGGAAATGGTAGAATTCTCAAGCGTCGGCCAATCTTAGTTGCGGCATCTGTGGGGAGCTATGGTGCTTATTTGGCTGATGGGTCTGAGTACAGTGGAAATTATGGTGAGGCTATGACGCTAGGAAGATTAAAAGATTTTCATCGGAGAAGGGTCCAAATCCTAGCAGAATCAGGTCCTGACCTACTTGCATTTGAAACAGTTCCAAATAAGCTGGAAGCTCAGGCTTATGCTGAGCTTTTGGAAGAAGAAAACATGCAACTTCCTGCATGGTTTTCTTTCAACTCTAAAGACGGCATCAATGTTGTTAGTGGTGATTCTCTCCTTGAATGCGCCACAGTTGCTGAATCATGCAAGAAAGTTGTTGCAGTTGGAATCAACTGCACTCCCCCTAGATTTATCCATGGACTGTTAACATTAATTACACAGGTGGCTACAAAACCAATTATTGTGTACCCAAATAGTGGTGAAAGCTATGATCCAGATAGAAAGATGTGGGTGCAAAACACTGGGGTTTCAGATGAAGATTTTGTCTCGTATGTAAACAAATGGTGTGAAGCAGGGGCTTCCCTTGTTGGAGGCTGTTGCAGAACAACTCCAAATACTATCAGAGCAATATACAGGACTCTTCCCAATAGGTCAACATCTCCACCTGAGCAGTAA

Protein Analysis

381

Amino Acids

41.12

Weight (kDa)

5.46

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 61 - 369 6.7e-82 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 944
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 179
AciI CCGC 4 cut(s) 120, 171, 179, 524
AclWI GGATC 2 cut(s) 230, 953
AcoI YGGCCR 1 cut(s) 509
AcsI RAATTY 1 cut(s) 495
AcvI CACGTG 1 cut(s) 10
AfaI GTAC 4 cut(s) 291, 460, 572, 935
AfiI CCNNNNNNNGG 1 cut(s) 944
AflIII ACRYGT 1 cut(s) 9
AgsI TTSAA 7 cut(s) 308, 356, 364, 419, 677, 763, 809
AluBI AGCT 7 cut(s) 208, 311, 540, 694, 701, 718, 954
AluI AGCT 7 cut(s) 208, 311, 540, 694, 701, 718, 954
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 1018
AlwI GGATC 2 cut(s) 230, 953
AlwNI CAGNNNCTG 2 cut(s) 659, 824
AoxI GGCC 3 cut(s) 345, 366, 509
ApeKI GCWGC 3 cut(s) 114, 117, 123
ApoI RAATTY 1 cut(s) 495
AseI ATTAAT 1 cut(s) 899
Asp700I GAANNNNTTC 1 cut(s) 681
AspLEI GCGC 4 cut(s) 128, 224, 251, 815
AspS9I GGNCC 2 cut(s) 634, 656
AsuHPI GGTGA 4 cut(s) 266, 599, 806, 959
AvaII GGWCC 2 cut(s) 634, 656
BanII GRGCYC 1 cut(s) 210
BauI CACGAG 1 cut(s) 435
BbrPI CACGTG 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 3 cut(s) 126, 129, 135
BccI CCATC 2 cut(s) 103, 554
BceAI ACGGC 2 cut(s) 235, 790
BcoDI GTCTC 1 cut(s) 1018
BfaI CTAG 3 cut(s) 602, 644, 873
BfmI CTRYAG 1 cut(s) 381
BfoI RGCGCY 3 cut(s) 129, 225, 252
BglI GCCNNNNNGGC 1 cut(s) 431
BisI GCNGC 5 cut(s) 115, 118, 121, 124, 525
BlpI GCTNAGC 1 cut(s) 714
BlsI GCNGC 5 cut(s) 116, 119, 122, 125, 526
Bme18I GGWCC 2 cut(s) 634, 656
BmgT120I GGNCC 2 cut(s) 634, 656
BmiI GGNNCC 1 cut(s) 635
BmrI ACTGGG 1 cut(s) 999
BmsI GCATC 3 cut(s) 344, 536, 786
BmuI ACTGGG 1 cut(s) 999
BpmI CTGGAG 1 cut(s) 265
Bpu10I CCTNAGC 3 cut(s) 404, 702, 1134
Bpu1102I GCTNAGC 1 cut(s) 714
BpuEI CTTGAG 1 cut(s) 485
BsaAI YACGTR 1 cut(s) 10
BsaBI GATNNNNATC 1 cut(s) 621
BsaJI CCNNGG 2 cut(s) 217, 883
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 4 cut(s) 165, 195, 529, 559
Bsc4I CCNNNNNNNGG 1 cut(s) 944
Bse1I ACTGG 1 cut(s) 994
Bse8I GATNNNNATC 1 cut(s) 621
BseDI CCNNGG 2 cut(s) 217, 883
BseGI GGATG 1 cut(s) 114
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 1 cut(s) 944
BseMII CTCAG 5 cut(s) 172, 558, 705, 716, 1125
BseNI ACTGG 1 cut(s) 994
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 3 cut(s) 126, 129, 135
BsgI GTGCAG 2 cut(s) 336, 847
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 3 cut(s) 347, 368, 511
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 944
BsmAI GTCTC 1 cut(s) 1018
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 815
BsnI GGCC 3 cut(s) 347, 368, 511
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 235, 958
Bsp1720I GCTNAGC 1 cut(s) 714
Bsp19I CCATGG 1 cut(s) 883
BspACI CCGC 4 cut(s) 120, 171, 179, 524
BspANI GGCC 3 cut(s) 347, 368, 511
BspCNI CTCAG 5 cut(s) 171, 559, 706, 715, 1126
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 1 cut(s) 635
BspPI GGATC 2 cut(s) 230, 953
BspQI GCTCTTC 1 cut(s) 475
BsrI ACTGG 1 cut(s) 994
BssECI CCNNGG 2 cut(s) 217, 883
BssMI GATC 2 cut(s) 235, 958
BssSI CACGAG 1 cut(s) 435
BssT1I CCWWGG 1 cut(s) 883
Bst2BI CACGAG 1 cut(s) 435
Bst4CI ACNGT 6 cut(s) 191, 482, 575, 682, 820, 891
Bst6I CTCTTC 3 cut(s) 381, 475, 1113
BstBAI YACGTR 1 cut(s) 10
BstC8I GCNNGC 2 cut(s) 165, 169
BstDEI CTNAG 7 cut(s) 158, 404, 517, 567, 702, 714, 1134
BstDSI CCRYGG 2 cut(s) 217, 883
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 1 cut(s) 179
BstH2I RGCGCY 3 cut(s) 129, 225, 252
BstHHI GCGC 4 cut(s) 128, 224, 251, 815
BstKTI GATC 2 cut(s) 238, 961
BstMAI GTCTC 1 cut(s) 1018
BstMBI GATC 2 cut(s) 235, 958
BstMWI GCNNNNNNNGC 6 cut(s) 120, 123, 255, 431, 554, 821
BstNSI RCATGY 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 381
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 126, 129, 135
BsuRI GGCC 3 cut(s) 347, 368, 511
BtgI CCRYGG 2 cut(s) 217, 883
BtsCI GGATG 1 cut(s) 114
BtsIMutI CAGTG 3 cut(s) 487, 580, 987
Cac8I GCNNGC 2 cut(s) 165, 169
CaiI CAGNNNCTG 2 cut(s) 659, 824
CfoI GCGC 4 cut(s) 128, 224, 251, 815
Cfr13I GGNCC 2 cut(s) 634, 656
CseI GACGC 2 cut(s) 494, 607
Csp6I GTAC 4 cut(s) 290, 459, 571, 934
CviAII CATG 4 cut(s) 736, 750, 831, 884
CviQI GTAC 4 cut(s) 290, 459, 571, 934
DdeI CTNAG 7 cut(s) 158, 404, 517, 567, 702, 714, 1134
DpnI GATC 2 cut(s) 237, 960
DpnII GATC 2 cut(s) 235, 958
EaeI YGGCCR 1 cut(s) 509
Eam1104I CTCTTC 3 cut(s) 381, 475, 1113
EarI CTCTTC 3 cut(s) 381, 475, 1113
EciI GGCGGA 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 883
Eco24I GRGCYC 1 cut(s) 210
Eco32I GATATC 2 cut(s) 322, 442
Eco47I GGWCC 2 cut(s) 634, 656
Eco53kI GAGCTC 1 cut(s) 208
Eco72I CACGTG 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 1 cut(s) 656
EcoRI GAATTC 1 cut(s) 495
EcoRV GATATC 2 cut(s) 322, 442
EcoT14I CCWWGG 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 883
FaeI CATG 4 cut(s) 739, 753, 834, 887
FaqI GGGAC 1 cut(s) 164
FatI CATG 4 cut(s) 735, 749, 830, 883
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 115, 118, 121, 124, 525
FokI GGATG 1 cut(s) 121
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 5 cut(s) 115, 118, 121, 124, 525
FspBI CTAG 3 cut(s) 602, 644, 873
GlaI GCGC 4 cut(s) 127, 223, 250, 814
GluI GCNGC 5 cut(s) 115, 118, 121, 124, 525
GsuI CTGGAG 1 cut(s) 265
HaeII RGCGCY 3 cut(s) 129, 225, 252
HaeIII GGCC 3 cut(s) 347, 368, 511
HapII CCGG 1 cut(s) 36
HgaI GACGC 2 cut(s) 494, 607
HhaI GCGC 4 cut(s) 128, 224, 251, 815
Hin1II CATG 4 cut(s) 739, 753, 834, 887
Hin6I GCGC 4 cut(s) 126, 222, 249, 813
HinP1I GCGC 4 cut(s) 126, 222, 249, 813
HincII GTYRAC 4 cut(s) 100, 187, 894, 1122
HindII GTYRAC 4 cut(s) 100, 187, 894, 1122
HinfI GANTC 7 cut(s) 174, 395, 650, 797, 827, 855, 1105
HpaI GTTAAC 1 cut(s) 894
HpaII CCGG 1 cut(s) 36
HphI GGTGA 4 cut(s) 266, 599, 806, 959
Hpy166II GTNNAC 8 cut(s) 100, 187, 194, 292, 894, 934, 1024, 1122
Hpy188I TCNGA 5 cut(s) 82, 568, 627, 1000, 1091
Hpy188III TCNNGA 3 cut(s) 244, 659, 962
Hpy8I GTNNAC 8 cut(s) 100, 187, 194, 292, 894, 934, 1024, 1122
Hpy99I CGWCG 5 cut(s) 107, 139, 142, 191, 510
HpyAV CCTTC 1 cut(s) 624
HpyCH4III ACNGT 6 cut(s) 191, 482, 575, 682, 820, 891
HpyCH4IV ACGT 2 cut(s) 9, 134
HpyCH4V TGCA 9 cut(s) 317, 671, 739, 749, 834, 848, 864, 982, 1068
HpyF10VI GCNNNNNNNGC 6 cut(s) 120, 123, 255, 431, 554, 821
HpyF3I CTNAG 7 cut(s) 158, 404, 517, 567, 702, 714, 1134
HpySE526I ACGT 2 cut(s) 9, 134
Hsp92II CATG 4 cut(s) 739, 753, 834, 887
HspAI GCGC 4 cut(s) 126, 222, 249, 813
KspAI GTTAAC 1 cut(s) 894
Kzo9I GATC 2 cut(s) 235, 958
LguI GCTCTTC 1 cut(s) 475
LmnI GCTCC 2 cut(s) 246, 537
Lsp1109I GCAGC 3 cut(s) 126, 129, 135
LweI GCATC 3 cut(s) 344, 536, 786
MaeI CTAG 3 cut(s) 602, 644, 873
MaeII ACGT 2 cut(s) 9, 134
MalI GATC 2 cut(s) 237, 960
MboI GATC 2 cut(s) 235, 958
MboII GAAGA 7 cut(s) 398, 462, 618, 737, 740, 1016, 1100
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 6 cut(s) 54, 420, 495, 580, 900, 924
MlyI GAGTC 2 cut(s) 183, 1099
MmeI TCCRAC 2 cut(s) 832, 1036
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 4 cut(s) 143, 611, 893, 899
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 815
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 6 cut(s) 120, 123, 255, 431, 554, 821
NcoI CCATGG 1 cut(s) 883
NdeII GATC 2 cut(s) 235, 958
NlaIII CATG 4 cut(s) 739, 753, 834, 887
NlaIV GGNNCC 1 cut(s) 635
NspI RCATGY 1 cut(s) 739
PciSI GCTCTTC 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 815
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 5 cut(s) 395, 650, 797, 827, 855
PflMI CCANNNNNTGG 1 cut(s) 944
PkrI GCNGC 5 cut(s) 116, 119, 122, 125, 526
PleI GAGTC 2 cut(s) 182, 1099
PmaCI CACGTG 1 cut(s) 10
PmlI CACGTG 1 cut(s) 10
PpsI GAGTC 2 cut(s) 182, 1099
Ppu21I YACGTR 1 cut(s) 10
PpuMI RGGWCCY 1 cut(s) 656
PshBI ATTAAT 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 656
PspCI CACGTG 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 635
PspPI GGNCC 2 cut(s) 634, 656
PspPPI RGGWCCY 1 cut(s) 656
PstNI CAGNNNCTG 2 cut(s) 659, 824
RsaI GTAC 4 cut(s) 291, 460, 572, 935
RsaNI GTAC 4 cut(s) 290, 459, 571, 934
SacI GAGCTC 1 cut(s) 210
SalI GTCGAC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 475
SaqAI TTAA 4 cut(s) 143, 611, 893, 899
SatI GCNGC 5 cut(s) 115, 118, 121, 124, 525
Sau3AI GATC 2 cut(s) 235, 958
Sau96I GGNCC 2 cut(s) 634, 656
SchI GAGTC 2 cut(s) 183, 1099
SduI GDGCHC 1 cut(s) 210
SfaNI GCATC 3 cut(s) 344, 536, 786
SfcI CTRYAG 1 cut(s) 381
SinI GGWCC 2 cut(s) 634, 656
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
Sse9I AATT 6 cut(s) 54, 420, 495, 580, 900, 924
SsiI CCGC 4 cut(s) 120, 171, 179, 524
SspMI CTAG 3 cut(s) 602, 644, 873
SstI GAGCTC 1 cut(s) 210
StyI CCWWGG 1 cut(s) 883
TaaI ACNGT 6 cut(s) 191, 482, 575, 682, 820, 891
TaiI ACGT 2 cut(s) 12, 137
TaqI TCGA 2 cut(s) 186, 210
TasI AATT 6 cut(s) 54, 420, 495, 580, 900, 924
TatI WGTACW 2 cut(s) 458, 570
TauI GCSGC 2 cut(s) 123, 527
TfiI GAWTC 5 cut(s) 395, 650, 797, 827, 855
Tru1I TTAA 4 cut(s) 143, 611, 893, 899
Tru9I TTAA 4 cut(s) 143, 611, 893, 899
TscAI CASTG 3 cut(s) 487, 580, 994
TseI GCWGC 3 cut(s) 114, 117, 123
TspDTI ATGAA 3 cut(s) 17, 611, 1017
TspRI CASTG 3 cut(s) 487, 580, 994
Van91I CCANNNNNTGG 1 cut(s) 944
VpaK11BI GGWCC 2 cut(s) 634, 656
VspI ATTAAT 1 cut(s) 899
XapI RAATTY 1 cut(s) 495
XceI RCATGY 1 cut(s) 739
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 3 cut(s) 602, 644, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.