pycom03g21270

DNA repair helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010853
Physical Location & Seq
Forward (+)
21578654 .. 21579971
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 879 bp
ATGGAGCCACCGTCCCTCTCCGTCTCAGTCAGAGCCAAAACCCAAAACCTGACAAAAACCATGAAGTTCCAGGTCGAAGACGTGACGGTCTACTTCCCGTACGACCACATATACCCGGAGCAATACGCTTACATGCTCGAGCTCAAGCGCGCCCTCGACGCCAAGGGTCACTGCCTCCTCGAGATGCCCACCGGCACCGGCAAAACCATCGCCCTTCTCTCTCTCATCACCTCCTACACTCTGTCCAAGCCCCAAAACCCCGTCAAGCTAATCTACTGCACCCGCACGGTTCACGAGATGGAGAAGACGCTCGCCGAGCTCAAGCTCCTCCACAATTACCAGGTCAAGCATCTGGGTCCGCAGGCTCAGATCTTGGCGGTGGGGCTTTCGTCGAGGAAGAATCTGTGTGTTAATCCGACGGTGTTGGCGGCGGAGAATCGGGATTCCGTGGATGCTGCGTGCCGGAAATTGACTGCCAGCTGGGTCAGGAACTTGGCGGCGGAAAACCCAAATGTGCCGACTTGTGAGTTTTTTGAGGAGTATGAGAGAGCTGGGTCTGGAGCTGTTTTGCCTCCTGGGGTTTATACATTGCAGGATTTGAGGGCGTATGGGAAGCAGAAAGGGTGGTGCCCCTACTTCTTGGCGAGGCATATGGTTCAGTTTTCGAATGTGGTGGTTTATAGTTATCAGTACATGCTTGATCCTAAAGTGGCTGGGATTATTTCCAAGGAAATGCAAAAGGAGTCTGTTGTGGTGTTTGATGAGGCTCATAACATTGATAATGTGTGCATCGAAGCGCTTAGTGTTAGCGTGAGGAGGCAGACAATTGAGGGTGCCAGGAGGAATCTCAGTAAGATGCAGCATGACATTGAAAGGTAG

Protein Analysis

293

Amino Acids

33.03

Weight (kDa)

8.56

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD_2 PF06733 92 - 275 2.8e-52 DEAD_2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AccB1I GGYRCC 3 cut(s) 194, 627, 833
AccI GTMKAC 1 cut(s) 90
AccII CGCG 1 cut(s) 150
AciI CCGC 7 cut(s) 283, 359, 377, 428, 431, 497, 500
AclWI GGATC 1 cut(s) 695
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 2 cut(s) 101, 692
AfeI AGCGCT 1 cut(s) 798
AgsI TTSAA 1 cut(s) 872
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 4 cut(s) 69, 339, 574, 836
AluBI AGCT 7 cut(s) 142, 268, 319, 325, 480, 551, 563
AluI AGCT 7 cut(s) 142, 268, 319, 325, 480, 551, 563
Alw21I GWGCWC 2 cut(s) 144, 321
Alw26I GTCTC 1 cut(s) 28
AlwI GGATC 1 cut(s) 695
Ama87I CYCGRG 2 cut(s) 137, 179
Aor51HI AGCGCT 1 cut(s) 798
ApeKI GCWGC 2 cut(s) 455, 859
AspLEI GCGC 3 cut(s) 150, 152, 799
AspS9I GGNCC 1 cut(s) 356
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 220
AsuII TTCGAA 1 cut(s) 665
AvaI CYCGRG 2 cut(s) 137, 179
AvaII GGWCC 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 632
BanI GGYRCC 3 cut(s) 194, 627, 833
BanII GRGCYC 2 cut(s) 144, 321
BauI CACGAG 1 cut(s) 293
BbsI GAAGAC 2 cut(s) 84, 311
Bbv12I GWGCWC 2 cut(s) 144, 321
BbvI GCAGC 2 cut(s) 442, 871
BccI CCATC 2 cut(s) 215, 292
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 4 cut(s) 71, 341, 576, 838
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 28
BfoI RGCGCY 1 cut(s) 800
BglII AGATCT 1 cut(s) 369
BisI GCNGC 4 cut(s) 429, 456, 498, 860
BlsI GCNGC 4 cut(s) 430, 457, 499, 861
Bme1390I CCNGG 5 cut(s) 71, 116, 341, 576, 838
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 2 cut(s) 137, 179
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 5 cut(s) 6, 196, 357, 629, 835
BmrFI CCNGG 5 cut(s) 71, 116, 341, 576, 838
BmsI GCATC 5 cut(s) 174, 358, 442, 798, 846
BpiI GAAGAC 2 cut(s) 84, 311
BpmI CTGGAG 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 665
BpuEI CTTGAG 2 cut(s) 128, 305
BpuMI CCSGG 1 cut(s) 116
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 4 cut(s) 162, 447, 575, 726
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse118I RCCGGY 2 cut(s) 191, 197
Bse3DI GCAATG 1 cut(s) 587
BseBI CCWGG 4 cut(s) 71, 341, 576, 838
BseDI CCNNGG 4 cut(s) 162, 447, 575, 726
BseGI GGATG 1 cut(s) 457
BseMI GCAATG 1 cut(s) 587
BseMII CTCAG 3 cut(s) 39, 380, 862
BsePI GCGCGC 1 cut(s) 148
BseRI GAGGAG 4 cut(s) 167, 317, 551, 829
BseSI GKGCMC 1 cut(s) 632
BseXI GCAGC 2 cut(s) 442, 871
BseYI CCCAGC 3 cut(s) 480, 551, 713
BsgI GTGCAG 1 cut(s) 262
Bsh1236I CGCG 1 cut(s) 150
BshNI GGYRCC 3 cut(s) 194, 627, 833
BsiHKAI GWGCWC 2 cut(s) 144, 321
BsiHKCI CYCGRG 2 cut(s) 137, 179
BsiSI CCGG 4 cut(s) 116, 192, 198, 463
BsiWI CGTACG 1 cut(s) 99
BsmAI GTCTC 1 cut(s) 28
BsmBI CGTCTC 1 cut(s) 28
BsoBI CYCGRG 2 cut(s) 137, 179
Bsp119I TTCGAA 1 cut(s) 665
Bsp1286I GDGCHC 3 cut(s) 144, 321, 632
Bsp143I GATC 2 cut(s) 369, 700
BspACI CCGC 7 cut(s) 283, 359, 377, 428, 431, 497, 500
BspCNI CTCAG 3 cut(s) 38, 379, 861
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 5 cut(s) 6, 196, 357, 629, 835
BspPI GGATC 1 cut(s) 695
BspT104I TTCGAA 1 cut(s) 665
BspT107I GGYRCC 3 cut(s) 194, 627, 833
BsrDI GCAATG 1 cut(s) 587
BsrFI RCCGGY 2 cut(s) 191, 197
BssAI RCCGGY 2 cut(s) 191, 197
BssECI CCNNGG 4 cut(s) 162, 447, 575, 726
BssHII GCGCGC 1 cut(s) 148
BssMI GATC 2 cut(s) 369, 700
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 2 cut(s) 162, 726
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 4 cut(s) 71, 341, 576, 838
Bst4CI ACNGT 4 cut(s) 12, 88, 289, 421
BstACI GRCGYC 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 665
BstC8I GCNNGC 5 cut(s) 150, 312, 363, 460, 478
BstDEI CTNAG 4 cut(s) 25, 366, 800, 848
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 1 cut(s) 457
BstFNI CGCG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 800
BstHHI GCGC 3 cut(s) 150, 152, 799
BstKTI GATC 2 cut(s) 372, 703
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 2 cut(s) 369, 700
BstMWI GCNNNNNNNGC 2 cut(s) 158, 316
BstNI CCWGG 4 cut(s) 71, 341, 576, 838
BstNSI RCATGY 2 cut(s) 136, 697
BstSCI CCNGG 5 cut(s) 69, 114, 339, 574, 836
BstSLI GKGCMC 1 cut(s) 632
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 442, 871
BstV2I GAAGAC 2 cut(s) 84, 311
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
BtgI CCRYGG 1 cut(s) 447
BtgZI GCGATG 1 cut(s) 193
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 457
BtsI GCAGTG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 5 cut(s) 150, 312, 363, 460, 478
CfoI GCGC 3 cut(s) 150, 152, 799
Cfr10I RCCGGY 2 cut(s) 191, 197
Cfr13I GGNCC 1 cut(s) 356
CseI GACGC 2 cut(s) 167, 316
CsiI ACCWGGT 1 cut(s) 339
Csp6I GTAC 2 cut(s) 100, 691
CviAII CATG 4 cut(s) 61, 133, 694, 863
CviQI GTAC 2 cut(s) 100, 691
DdeI CTNAG 4 cut(s) 25, 366, 800, 848
DpnI GATC 2 cut(s) 371, 702
DpnII GATC 2 cut(s) 369, 700
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
EciI GGCGGA 2 cut(s) 446, 515
Ecl136II GAGCTC 2 cut(s) 142, 319
Eco130I CCWWGG 2 cut(s) 162, 726
Eco24I GRGCYC 2 cut(s) 144, 321
Eco47I GGWCC 1 cut(s) 356
Eco47III AGCGCT 1 cut(s) 798
Eco53kI GAGCTC 2 cut(s) 142, 319
Eco88I CYCGRG 2 cut(s) 137, 179
EcoICRI GAGCTC 2 cut(s) 142, 319
EcoRII CCWGG 4 cut(s) 69, 339, 574, 836
EcoT14I CCWWGG 2 cut(s) 162, 726
EcoT38I GRGCYC 2 cut(s) 144, 321
ErhI CCWWGG 2 cut(s) 162, 726
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 4 cut(s) 64, 136, 697, 866
FatI CATG 4 cut(s) 60, 132, 693, 862
FauI CCCGC 1 cut(s) 290
FauNDI CATATG 1 cut(s) 651
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 4 cut(s) 429, 456, 498, 860
FokI GGATG 1 cut(s) 464
FriOI GRGCYC 2 cut(s) 144, 321
Fsp4HI GCNGC 4 cut(s) 429, 456, 498, 860
GlaI GCGC 3 cut(s) 149, 151, 798
GluI GCNGC 4 cut(s) 429, 456, 498, 860
GsaI CCCAGC 3 cut(s) 484, 555, 717
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 800
HapII CCGG 4 cut(s) 116, 192, 198, 463
HgaI GACGC 2 cut(s) 167, 316
HhaI GCGC 3 cut(s) 150, 152, 799
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 4 cut(s) 64, 136, 697, 866
Hin6I GCGC 3 cut(s) 148, 150, 797
HinP1I GCGC 3 cut(s) 148, 150, 797
HinfI GANTC 5 cut(s) 400, 436, 443, 743, 844
HpaII CCGG 4 cut(s) 116, 192, 198, 463
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 2 cut(s) 91, 292
Hpy188I TCNGA 3 cut(s) 32, 369, 417
Hpy188III TCNNGA 5 cut(s) 181, 293, 440, 487, 558
Hpy8I GTNNAC 2 cut(s) 91, 292
Hpy99I CGWCG 3 cut(s) 161, 394, 421
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 4 cut(s) 12, 88, 289, 421
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 5 cut(s) 279, 592, 736, 789, 859
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 316
HpyF3I CTNAG 4 cut(s) 25, 366, 800, 848
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 4 cut(s) 64, 136, 697, 866
HspAI GCGC 3 cut(s) 148, 150, 797
Kzo9I GATC 2 cut(s) 369, 700
LmnI GCTCC 4 cut(s) 4, 118, 330, 560
Lsp1109I GCAGC 2 cut(s) 442, 871
LweI GCATC 5 cut(s) 174, 358, 442, 798, 846
MabI ACCWGGT 1 cut(s) 339
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 2 cut(s) 82, 167
MalI GATC 2 cut(s) 371, 702
MboI GATC 2 cut(s) 369, 700
MboII GAAGA 3 cut(s) 89, 316, 409
MfeI CAATTG 1 cut(s) 825
MflI RGATCY 1 cut(s) 369
MhlI GDGCHC 3 cut(s) 144, 321, 632
MluCI AATT 3 cut(s) 334, 467, 825
MlyI GAGTC 1 cut(s) 752
MmeI TCCRAC 1 cut(s) 440
MseI TTAA 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 480
MspI CCGG 4 cut(s) 116, 192, 198, 463
MspR9I CCNGG 5 cut(s) 71, 116, 341, 576, 838
MunI CAATTG 1 cut(s) 825
MvaI CCWGG 4 cut(s) 71, 341, 576, 838
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 2 cut(s) 158, 316
NciI CCSGG 1 cut(s) 116
NdeI CATATG 1 cut(s) 651
NdeII GATC 2 cut(s) 369, 700
NlaIII CATG 4 cut(s) 64, 136, 697, 866
NlaIV GGNNCC 5 cut(s) 6, 196, 357, 629, 835
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 2 cut(s) 82, 167
NspI RCATGY 2 cut(s) 136, 697
NspV TTCGAA 1 cut(s) 665
PaeR7I CTCGAG 2 cut(s) 137, 179
PauI GCGCGC 1 cut(s) 148
PfeI GAWTC 4 cut(s) 400, 436, 443, 844
Pfl23II CGTACG 1 cut(s) 99
PkrI GCNGC 4 cut(s) 430, 457, 499, 861
PleI GAGTC 1 cut(s) 751
PpsI GAGTC 1 cut(s) 751
Psp124BI GAGCTC 2 cut(s) 144, 321
Psp6I CCWGG 4 cut(s) 69, 339, 574, 836
PspFI CCCAGC 3 cut(s) 480, 551, 713
PspGI CCWGG 4 cut(s) 69, 339, 574, 836
PspLI CGTACG 1 cut(s) 99
PspN4I GGNNCC 5 cut(s) 6, 196, 357, 629, 835
PspPI GGNCC 1 cut(s) 356
PspXI VCTCGAGB 1 cut(s) 137
PsuI RGATCY 1 cut(s) 369
PteI GCGCGC 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 480
RsaI GTAC 2 cut(s) 101, 692
RsaNI GTAC 2 cut(s) 100, 691
SacI GAGCTC 2 cut(s) 144, 321
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 4 cut(s) 429, 456, 498, 860
Sau3AI GATC 2 cut(s) 369, 700
Sau96I GGNCC 1 cut(s) 356
SchI GAGTC 1 cut(s) 752
ScrFI CCNGG 5 cut(s) 71, 116, 341, 576, 838
SduI GDGCHC 3 cut(s) 144, 321, 632
SexAI ACCWGGT 1 cut(s) 339
SfaNI GCATC 5 cut(s) 174, 358, 442, 798, 846
Sfr274I CTCGAG 2 cut(s) 137, 179
SfuI TTCGAA 1 cut(s) 665
SinI GGWCC 1 cut(s) 356
SlaI CTCGAG 2 cut(s) 137, 179
SmlI CTYRAG 4 cut(s) 137, 143, 179, 320
SmoI CTYRAG 4 cut(s) 137, 143, 179, 320
Sse9I AATT 3 cut(s) 334, 467, 825
SsiI CCGC 7 cut(s) 283, 359, 377, 428, 431, 497, 500
SstI GAGCTC 2 cut(s) 144, 321
StyD4I CCNGG 5 cut(s) 69, 114, 339, 574, 836
StyI CCWWGG 2 cut(s) 162, 726
TaaI ACNGT 4 cut(s) 12, 88, 289, 421
TaiI ACGT 1 cut(s) 84
TaqI TCGA 7 cut(s) 75, 138, 156, 180, 392, 665, 792
TasI AATT 3 cut(s) 334, 467, 825
TatI WGTACW 1 cut(s) 690
TauI GCSGC 2 cut(s) 431, 500
TfiI GAWTC 4 cut(s) 400, 436, 443, 844
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 2 cut(s) 82, 167
TseI GCWGC 2 cut(s) 455, 859
Tsp45I GTSAC 2 cut(s) 82, 167
TspDTI ATGAA 1 cut(s) 77
TspGWI ACGGA 2 cut(s) 10, 436
TspRI CASTG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 356
XceI RCATGY 2 cut(s) 136, 697
XhoI CTCGAG 2 cut(s) 137, 179
XmiI GTMKAC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.