pycom04g02460

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011554
Physical Location & Seq
Forward (+)
2183740 .. 2187635
3896 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g02460.3

Sequence Viewer

Length: 1224 bp
ATGGTGGGGCTGGAGTGCTTTCTGGACGTCGTCAGGGCCCTCGGCATGGGCTCCTGCCTCTCTGCTGATGGCAGCGCTGCCGCCTACGGCTCCGTCCCTTCATCCGACAGCAAGAGGCGGATGAGGAGGGTGGCTGCGATTTCCTCCCCGTCCTCAACGACACGGTCAGCCGATAACGTGGAGATGTGGCTGCACAGAGTCCATGACAGGATGTTCTTGAATGGGTCCTCTGACTTTACTTCCTTGTTCAGCAAGCAAGGCAAGAAAGGGGTCAATCAGGACGCCATGATTGCTTGGGAGAATTTCGGTTCGGCGGATACCACTTTCTGTGGTGTTTTTGATGGGCATGGTCCTTTTGGCCATGTGGTTGCAAAGAAGGTGAGGGATGCTCTACCTTTGCGGCTGACTTCACAGTGGGTATTGAATGGTAATGCCAGGAACGGGCCTGCTGATGATTCGAGCGAGGCTGTTGCATATCACGAAGGCAAGGATGAGGAGCGCTGTGATCTTTTTATGACATTGAAAGATTCATTTTTGAAGGCTTTTAAGGTCATGGACAAGGAGCTCAAATTACACCCCCGTATTGATTGTTATTGCAGCGGGACAACTGCTGTCACCTTGGTCAAGCAGGGTCAGGATCTTGTTATTGCAAATGTTGGGGACTCTAGAGCTGTATTGGGCACAAGAGATAAAGATGGTTCCCTCGTTGCTGTTCAGTTGACTGTAGACCTCAAACCAAATCTTCCAAGGGAAGCAGAGAGGATCAGGCTATGCAAGGGACGAGTATTTGCTCTCCAAAATGAGCCTGAGATCGCTCGAGTTTGGTTGCCCAACTGTAATTCACCTGGCCTGGCCATGGCACGGGCTTTTGGAGATTTCTGCCTAAAGGACTTTGGCTTAATCTCTGTCCCTGATATATCTTATCGTCGCCTCACTGAGAAGGATGAATTTGTAGTGTTAGCTACGGATGGGATATGGGATGTCCTCTCAAATGAGGAAGTGGTGGATATAGTAGCATTAGCTCCACGACCATCTGCTGCTCGAGCCCTGGTAGAGTCAGCAACTCGTGCTTGGAGGTTAAAATATCCGTATGCCAAGGTTGATGATTGTGCTGTGGTTTGCCTCTTCCTCAACTCCAGTACATCAAACGCTACTTCCCATTTCTCGCCAACCGGAGAGGAAATGAGCTCTGGAAAATCGCTGCAGTATCCGGAAGGGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

408

Amino Acids

44.42

Weight (kDa)

6.21

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
AccI GTMKAC 1 cut(s) 726
AccIII TCCGGA 1 cut(s) 1210
AciI CCGC 5 cut(s) 81, 118, 314, 400, 600
AclWI GGATC 2 cut(s) 645, 770
AcoI YGGCCR 2 cut(s) 358, 852
AcsI RAATTY 2 cut(s) 301, 947
AcyI GRCGYC 2 cut(s) 27, 282
AfaI GTAC 1 cut(s) 1141
AfeI AGCGCT 2 cut(s) 76, 500
AfiI CCNNNNNNNGG 4 cut(s) 46, 441, 856, 861
AgsI TTSAA 4 cut(s) 220, 424, 523, 538
AjnI CCWGG 4 cut(s) 434, 844, 849, 1047
AluBI AGCT 5 cut(s) 565, 671, 962, 1022, 1188
AluI AGCT 5 cut(s) 565, 671, 962, 1022, 1188
Alw21I GWGCWC 2 cut(s) 567, 1190
AlwI GGATC 2 cut(s) 645, 770
Ama87I CYCGRG 2 cut(s) 816, 1041
Aor13HI TCCGGA 1 cut(s) 1210
Aor51HI AGCGCT 2 cut(s) 76, 500
AoxI GGCC 5 cut(s) 36, 358, 443, 847, 852
ApaI GGGCCC 1 cut(s) 40
ApeKI GCWGC 7 cut(s) 72, 77, 134, 190, 597, 1037, 1201
ApoI RAATTY 2 cut(s) 301, 947
AspLEI GCGC 2 cut(s) 77, 501
AspS9I GGNCC 5 cut(s) 36, 37, 225, 350, 443
AsuHPI GGTGA 3 cut(s) 391, 607, 834
AvaI CYCGRG 2 cut(s) 816, 1041
AvaII GGWCC 2 cut(s) 225, 350
BaeGI GKGCMC 2 cut(s) 40, 683
BalI TGGCCA 2 cut(s) 360, 854
BanII GRGCYC 5 cut(s) 40, 53, 567, 1048, 1190
BauI CACGAG 1 cut(s) 1065
Bbv12I GWGCWC 2 cut(s) 567, 1190
BbvI GCAGC 7 cut(s) 64, 84, 121, 177, 609, 1024, 1188
BccI CCATC 5 cut(s) 62, 335, 689, 962, 1039
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 4 cut(s) 436, 846, 851, 1049
BciVI GTATCC 2 cut(s) 310, 1218
BfaI CTAG 1 cut(s) 666
BfmI CTRYAG 2 cut(s) 723, 1202
BfoI RGCGCY 2 cut(s) 78, 502
BfuI GTATCC 2 cut(s) 310, 1218
BisI GCNGC 9 cut(s) 73, 78, 81, 135, 191, 401, 598, 1038, 1202
BlsI GCNGC 9 cut(s) 74, 79, 82, 136, 192, 402, 599, 1039, 1203
Bme1390I CCNGG 4 cut(s) 436, 846, 851, 1049
Bme18I GGWCC 2 cut(s) 225, 350
BmeT110I CYCGRG 2 cut(s) 816, 1041
BmgT120I GGNCC 5 cut(s) 36, 37, 225, 350, 443
BmiI GGNNCC 5 cut(s) 38, 52, 91, 226, 700
BmrFI CCNGG 4 cut(s) 436, 846, 851, 1049
BmsI GCATC 1 cut(s) 376
BplI GAGNNNNNCTC 2 cut(s) 373, 405
BpmI CTGGAG 2 cut(s) 32, 1120
BsaHI GRCGYC 2 cut(s) 27, 282
BsaJI CCNNGG 6 cut(s) 40, 618, 746, 855, 1047, 1095
BsaWI WCCGGW 2 cut(s) 1172, 1210
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 441, 856, 861
Bse1I ACTGG 1 cut(s) 1137
BseAI TCCGGA 1 cut(s) 1210
BseBI CCWGG 4 cut(s) 436, 846, 851, 1049
BseDI CCNNGG 6 cut(s) 40, 618, 746, 855, 1047, 1095
BseGI GGATG 8 cut(s) 101, 126, 216, 391, 496, 949, 973, 985
BseLI CCNNNNNNNGG 4 cut(s) 46, 441, 856, 861
BseMII CTCAG 2 cut(s) 798, 927
BseNI ACTGG 1 cut(s) 1137
BseRI GAGGAG 2 cut(s) 139, 509
BseSI GKGCMC 2 cut(s) 40, 683
BseXI GCAGC 7 cut(s) 64, 84, 121, 177, 609, 1024, 1188
BsgI GTGCAG 1 cut(s) 176
BshFI GGCC 5 cut(s) 38, 360, 445, 849, 854
BsiHKAI GWGCWC 2 cut(s) 567, 1190
BsiHKCI CYCGRG 2 cut(s) 816, 1041
BsiSI CCGG 2 cut(s) 1173, 1211
BslFI GGGAC 5 cut(s) 80, 616, 674, 792, 893
BslI CCNNNNNNNGG 4 cut(s) 46, 441, 856, 861
BsmFI GGGAC 5 cut(s) 80, 616, 674, 792, 893
BsnI GGCC 5 cut(s) 38, 360, 445, 849, 854
BsoBI CYCGRG 2 cut(s) 816, 1041
Bsp120I GGGCCC 1 cut(s) 36
Bsp1286I GDGCHC 6 cut(s) 40, 53, 567, 683, 1048, 1190
Bsp13I TCCGGA 1 cut(s) 1210
Bsp143I GATC 4 cut(s) 505, 637, 762, 810
Bsp19I CCATGG 1 cut(s) 855
BspACI CCGC 5 cut(s) 81, 118, 314, 400, 600
BspANI GGCC 5 cut(s) 38, 360, 445, 849, 854
BspCNI CTCAG 2 cut(s) 799, 928
BspEI TCCGGA 1 cut(s) 1210
BspLI GGNNCC 5 cut(s) 38, 52, 91, 226, 700
BspMAI CTGCAG 1 cut(s) 1206
BspPI GGATC 2 cut(s) 645, 770
BsrI ACTGG 1 cut(s) 1137
BssECI CCNNGG 6 cut(s) 40, 618, 746, 855, 1047, 1095
BssMI GATC 4 cut(s) 505, 637, 762, 810
BssNI GRCGYC 2 cut(s) 27, 282
BssSI CACGAG 1 cut(s) 1065
BssT1I CCWWGG 4 cut(s) 618, 746, 855, 1095
Bst2BI CACGAG 1 cut(s) 1065
Bst2UI CCWGG 4 cut(s) 436, 846, 851, 1049
Bst4CI ACNGT 4 cut(s) 165, 414, 724, 836
Bst6I CTCTTC 1 cut(s) 1130
BstACI GRCGYC 2 cut(s) 27, 282
BstAPI GCANNNNNTGC 1 cut(s) 1067
BstC8I GCNNGC 2 cut(s) 254, 447
BstDEI CTNAG 2 cut(s) 807, 936
BstDSI CCRYGG 1 cut(s) 855
BstF5I GGATG 8 cut(s) 101, 126, 216, 391, 496, 949, 973, 985
BstH2I RGCGCY 2 cut(s) 78, 502
BstHHI GCGC 2 cut(s) 77, 501
BstKTI GATC 4 cut(s) 508, 640, 765, 813
BstMBI GATC 4 cut(s) 505, 637, 762, 810
BstMWI GCNNNNNNNGC 4 cut(s) 258, 290, 1043, 1067
BstNI CCWGG 4 cut(s) 436, 846, 851, 1049
BstSCI CCNGG 4 cut(s) 434, 844, 849, 1047
BstSFI CTRYAG 2 cut(s) 723, 1202
BstSLI GKGCMC 2 cut(s) 40, 683
BstV1I GCAGC 7 cut(s) 64, 84, 121, 177, 609, 1024, 1188
BstX2I RGATCY 1 cut(s) 637
BstYI RGATCY 1 cut(s) 637
BsuI GTATCC 2 cut(s) 310, 1218
BsuRI GGCC 5 cut(s) 38, 360, 445, 849, 854
BtgI CCRYGG 1 cut(s) 855
BtsCI GGATG 8 cut(s) 101, 126, 216, 391, 496, 949, 973, 985
BtsIMutI CAGTG 2 cut(s) 419, 933
Cac8I GCNNGC 2 cut(s) 254, 447
CfoI GCGC 2 cut(s) 77, 501
Cfr13I GGNCC 5 cut(s) 36, 37, 225, 350, 443
CseI GACGC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 1140
CviAII CATG 7 cut(s) 46, 203, 286, 347, 362, 553, 856
CviQI GTAC 1 cut(s) 1140
DdeI CTNAG 2 cut(s) 807, 936
DpnI GATC 4 cut(s) 507, 639, 764, 812
DpnII GATC 4 cut(s) 505, 637, 762, 810
EaeI YGGCCR 2 cut(s) 358, 852
Eam1104I CTCTTC 1 cut(s) 1130
EarI CTCTTC 1 cut(s) 1130
EciI GGCGGA 2 cut(s) 133, 329
Ecl136II GAGCTC 2 cut(s) 565, 1188
Eco130I CCWWGG 4 cut(s) 618, 746, 855, 1095
Eco24I GRGCYC 5 cut(s) 40, 53, 567, 1048, 1190
Eco47I GGWCC 2 cut(s) 225, 350
Eco47III AGCGCT 2 cut(s) 76, 500
Eco53kI GAGCTC 2 cut(s) 565, 1188
Eco88I CYCGRG 2 cut(s) 816, 1041
EcoICRI GAGCTC 2 cut(s) 565, 1188
EcoO109I RGGNCCY 3 cut(s) 36, 37, 225
EcoRII CCWGG 4 cut(s) 434, 844, 849, 1047
EcoT14I CCWWGG 4 cut(s) 618, 746, 855, 1095
EcoT38I GRGCYC 5 cut(s) 40, 53, 567, 1048, 1190
ErhI CCWWGG 4 cut(s) 618, 746, 855, 1095
FaeI CATG 7 cut(s) 49, 206, 289, 350, 365, 556, 859
FaqI GGGAC 5 cut(s) 80, 616, 674, 792, 893
FatI CATG 7 cut(s) 45, 202, 285, 346, 361, 552, 855
FauI CCCGC 1 cut(s) 593
FblI GTMKAC 1 cut(s) 726
Fnu4HI GCNGC 9 cut(s) 73, 78, 81, 135, 191, 401, 598, 1038, 1202
FokI GGATG 8 cut(s) 88, 133, 223, 398, 503, 956, 980, 992
FriOI GRGCYC 5 cut(s) 40, 53, 567, 1048, 1190
Fsp4HI GCNGC 9 cut(s) 73, 78, 81, 135, 191, 401, 598, 1038, 1202
FspBI CTAG 1 cut(s) 666
GlaI GCGC 2 cut(s) 76, 500
GluI GCNGC 9 cut(s) 73, 78, 81, 135, 191, 401, 598, 1038, 1202
GsuI CTGGAG 2 cut(s) 32, 1120
HaeII RGCGCY 2 cut(s) 78, 502
HaeIII GGCC 5 cut(s) 38, 360, 445, 849, 854
HapII CCGG 2 cut(s) 1173, 1211
HgaI GACGC 1 cut(s) 290
HhaI GCGC 2 cut(s) 77, 501
Hin1I GRCGYC 2 cut(s) 27, 282
Hin1II CATG 7 cut(s) 49, 206, 289, 350, 365, 556, 859
Hin6I GCGC 2 cut(s) 75, 499
HinP1I GCGC 2 cut(s) 75, 499
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HinfI GANTC 5 cut(s) 198, 455, 527, 662, 1055
HpaII CCGG 2 cut(s) 1173, 1211
HphI GGTGA 3 cut(s) 391, 607, 834
Hpy166II GTNNAC 2 cut(s) 720, 727
Hpy188I TCNGA 2 cut(s) 106, 232
Hpy188III TCNNGA 8 cut(s) 23, 217, 278, 479, 635, 666, 1191, 1211
Hpy8I GTNNAC 2 cut(s) 720, 727
Hpy99I CGWCG 2 cut(s) 32, 930
HpyAV CCTTC 6 cut(s) 108, 370, 476, 532, 934, 1208
HpyCH4III ACNGT 4 cut(s) 165, 414, 724, 836
HpyCH4IV ACGT 2 cut(s) 27, 177
HpyCH4V TGCA 7 cut(s) 193, 371, 473, 597, 650, 774, 1204
HpyF10VI GCNNNNNNNGC 4 cut(s) 258, 290, 1043, 1067
HpyF3I CTNAG 2 cut(s) 807, 936
HpySE526I ACGT 2 cut(s) 27, 177
Hsp92I GRCGYC 2 cut(s) 27, 282
Hsp92II CATG 7 cut(s) 49, 206, 289, 350, 365, 556, 859
HspAI GCGC 2 cut(s) 75, 499
Kpn2I TCCGGA 1 cut(s) 1210
Kzo9I GATC 4 cut(s) 505, 637, 762, 810
LmnI GCTCC 5 cut(s) 56, 95, 496, 562, 1027
Lsp1109I GCAGC 7 cut(s) 64, 84, 121, 177, 609, 1024, 1188
LweI GCATC 1 cut(s) 376
MaeI CTAG 1 cut(s) 666
MaeII ACGT 2 cut(s) 27, 177
MaeIII GTNAC 1 cut(s) 613
MalI GATC 4 cut(s) 507, 639, 764, 812
MboI GATC 4 cut(s) 505, 637, 762, 810
MboII GAAGA 2 cut(s) 734, 1117
MflI RGATCY 1 cut(s) 637
MhlI GDGCHC 6 cut(s) 40, 53, 567, 683, 1048, 1190
MlsI TGGCCA 2 cut(s) 360, 854
MluCI AATT 4 cut(s) 301, 569, 838, 947
MluNI TGGCCA 2 cut(s) 360, 854
MlyI GAGTC 3 cut(s) 207, 656, 1064
MmeI TCCRAC 1 cut(s) 129
Mox20I TGGCCA 2 cut(s) 360, 854
MroI TCCGGA 1 cut(s) 1210
MscI TGGCCA 2 cut(s) 360, 854
MseI TTAA 3 cut(s) 546, 899, 1079
Msp20I TGGCCA 2 cut(s) 360, 854
MspA1I CMGCKG 1 cut(s) 600
MspI CCGG 2 cut(s) 1173, 1211
MspR9I CCNGG 4 cut(s) 436, 846, 851, 1049
MvaI CCWGG 4 cut(s) 436, 846, 851, 1049
MwoI GCNNNNNNNGC 4 cut(s) 258, 290, 1043, 1067
NcoI CCATGG 1 cut(s) 855
NdeII GATC 4 cut(s) 505, 637, 762, 810
NlaIII CATG 7 cut(s) 49, 206, 289, 350, 365, 556, 859
NlaIV GGNNCC 5 cut(s) 38, 52, 91, 226, 700
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 1 cut(s) 613
PaeR7I CTCGAG 2 cut(s) 816, 1041
PfeI GAWTC 2 cut(s) 455, 527
PflFI GACNNNGTC 2 cut(s) 29, 163
PkrI GCNGC 9 cut(s) 74, 79, 82, 136, 192, 402, 599, 1039, 1203
PleI GAGTC 3 cut(s) 206, 656, 1063
PpsI GAGTC 3 cut(s) 206, 656, 1063
PpuMI RGGWCCY 1 cut(s) 225
Psp124BI GAGCTC 2 cut(s) 567, 1190
Psp5II RGGWCCY 1 cut(s) 225
Psp6I CCWGG 4 cut(s) 434, 844, 849, 1047
PspGI CCWGG 4 cut(s) 434, 844, 849, 1047
PspN4I GGNNCC 5 cut(s) 38, 52, 91, 226, 700
PspOMI GGGCCC 1 cut(s) 36
PspPI GGNCC 5 cut(s) 36, 37, 225, 350, 443
PspPPI RGGWCCY 1 cut(s) 225
PspXI VCTCGAGB 2 cut(s) 816, 1041
PstI CTGCAG 1 cut(s) 1206
PsuI RGATCY 1 cut(s) 637
PsyI GACNNNGTC 2 cut(s) 29, 163
RsaI GTAC 1 cut(s) 1141
RsaNI GTAC 1 cut(s) 1140
SacI GAGCTC 2 cut(s) 567, 1190
SaqAI TTAA 3 cut(s) 546, 899, 1079
SatI GCNGC 9 cut(s) 73, 78, 81, 135, 191, 401, 598, 1038, 1202
Sau3AI GATC 4 cut(s) 505, 637, 762, 810
Sau96I GGNCC 5 cut(s) 36, 37, 225, 350, 443
SchI GAGTC 3 cut(s) 207, 656, 1064
ScrFI CCNGG 4 cut(s) 436, 846, 851, 1049
SduI GDGCHC 6 cut(s) 40, 53, 567, 683, 1048, 1190
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 2 cut(s) 723, 1202
Sfr274I CTCGAG 2 cut(s) 816, 1041
SinI GGWCC 2 cut(s) 225, 350
SlaI CTCGAG 2 cut(s) 816, 1041
SmlI CTYRAG 2 cut(s) 816, 1041
SmoI CTYRAG 2 cut(s) 816, 1041
Sse9I AATT 4 cut(s) 301, 569, 838, 947
SsiI CCGC 5 cut(s) 81, 118, 314, 400, 600
SspMI CTAG 1 cut(s) 666
SstI GAGCTC 2 cut(s) 567, 1190
StyD4I CCNGG 4 cut(s) 434, 844, 849, 1047
StyI CCWWGG 4 cut(s) 618, 746, 855, 1095
TaaI ACNGT 4 cut(s) 165, 414, 724, 836
TaiI ACGT 2 cut(s) 30, 180
TaqI TCGA 3 cut(s) 458, 817, 1042
TasI AATT 4 cut(s) 301, 569, 838, 947
TatI WGTACW 1 cut(s) 1139
TauI GCSGC 2 cut(s) 83, 403
TfiI GAWTC 2 cut(s) 455, 527
Tru1I TTAA 3 cut(s) 546, 899, 1079
Tru9I TTAA 3 cut(s) 546, 899, 1079
TscAI CASTG 2 cut(s) 419, 940
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 7 cut(s) 72, 77, 134, 190, 597, 1037, 1201
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 3 cut(s) 90, 519, 960
TspGWI ACGGA 3 cut(s) 82, 980, 1077
TspRI CASTG 2 cut(s) 419, 940
Tth111I GACNNNGTC 2 cut(s) 29, 163
VpaK11BI GGWCC 2 cut(s) 225, 350
XapI RAATTY 2 cut(s) 301, 947
XbaI TCTAGA 1 cut(s) 665
XhoI CTCGAG 2 cut(s) 816, 1041
XmiI GTMKAC 1 cut(s) 726
XspI CTAG 1 cut(s) 666
ZraI GACGTC 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.