Rh4BG158800

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011554
Physical Location & Seq
Reverse (-)
28674030 .. 28680370
6341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG158800.1

Sequence Viewer

Length: 876 bp
ATGGTTGGTCCGGTGGGGGAGGCAAGAAGGGGTGGACATGACTGTGTGGTTGGGTTGGACTGTTTTCTGGACATCGTCAGGGCTTTAGGGATGGGCTCCTGCCTCTCCATCACCGGTGACAACGCCCCCGGCTACGGCCGGGTGTCTTCCCCGACGCAGAGCAATAAGAGGAGGAAGAGGTTGACCGGCAACTCGTCGTCACCGGTTTCGTCGGCGGCGACGAGTTCCATGGAGATGTGGCTGCACAGGATTCCGGGGAGGTTGCTCCTGAATGGCTTCTCTGATGTGGCTTCCCTGTTCAGCAAGCAAGGCAAGAAAGGTGTCAATCAGGACGCTATGATTGTTTGGGAGAATTTCGGCTCCAAGGAAGATACAATATTTTGTGGTGTTTTTGATGGCCATGGTCCTAGTGGTCATATGGTGGCGAAGAAGGTGAGGGATTCTCTACCTTTGAAGGTCAGCTCACAATGGGTGTTGAACACCAGCGCCGCGGATACTAACCACGCCGGTGCGTCTGTGGAGAACAAGGACAGCCATGGTGAATTTTTCGCCACATTGAAAGATTCGTTTTTGAAGGCTTTTAAGGTCATGGACAAGGAGCTTAAGTTGCATCCTCGTATTGATTGCTATTGCAGTGGGACAACAGCAGTTACGTTGGTCAAGCAGGGTCAGGATCTTATTATTGCAAATATTGGGGACTCTAGAGCTGTGCTAGGCACAAGAGATGAAGGGGGTTCTCTCGTTGCAGTTCAGTTGACTGTAGACTTAAAACCAAATCTTCCAAGTAACTTTCTTTCTCTTAATTTAGTTAGAGTACAAAAATGTTGTTTTATGTTTATGTTAAAGACTGCTGCATTTGATTACCCTTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

291

Amino Acids

31.47

Weight (kDa)

8.88

Isoelectric Point (pI)

28.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 103 - 261 3.2e-14 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 762
AccII CGCG 1 cut(s) 491
AciI CCGC 3 cut(s) 215, 489, 491
AclWI GGATC 1 cut(s) 681
AcoI YGGCCR 2 cut(s) 136, 397
AcsI RAATTY 2 cut(s) 352, 542
AfaI GTAC 1 cut(s) 816
AfiI CCNNNNNNNGG 1 cut(s) 134
AflII CTTAAG 1 cut(s) 602
AgeI ACCGGT 2 cut(s) 113, 202
AgsI TTSAA 4 cut(s) 454, 478, 559, 574
AleI CACNNNNGTG 1 cut(s) 507
AluBI AGCT 3 cut(s) 462, 601, 707
AluI AGCT 3 cut(s) 462, 601, 707
AlwI GGATC 1 cut(s) 681
AoxI GGCC 2 cut(s) 136, 397
ApeKI GCWGC 2 cut(s) 241, 851
ApoI RAATTY 2 cut(s) 352, 542
AsiGI ACCGGT 2 cut(s) 113, 202
Asp700I GAANNNNTTC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 8, 404
AsuC2I CCSGG 3 cut(s) 129, 140, 255
AsuHPI GGTGA 5 cut(s) 103, 128, 192, 445, 551
AvaII GGWCC 2 cut(s) 8, 404
BalI TGGCCA 1 cut(s) 399
BanII GRGCYC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 228, 838
BccI CCATC 3 cut(s) 85, 116, 389
BceAI ACGGC 1 cut(s) 151
BciVI GTATCC 1 cut(s) 487
BcnI CCSGG 3 cut(s) 129, 140, 255
BfaI CTAG 3 cut(s) 408, 702, 713
BfmI CTRYAG 1 cut(s) 759
BfoI RGCGCY 1 cut(s) 489
BfrI CTTAAG 1 cut(s) 602
BfuI GTATCC 1 cut(s) 487
BisI GCNGC 4 cut(s) 216, 242, 489, 852
BlsI GCNGC 4 cut(s) 217, 243, 490, 853
Bme1390I CCNGG 3 cut(s) 129, 140, 255
Bme18I GGWCC 2 cut(s) 8, 404
BmgT120I GGNCC 2 cut(s) 8, 404
BmiI GGNNCC 2 cut(s) 97, 361
BmrFI CCNGG 3 cut(s) 129, 140, 255
BmsI GCATC 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 138
BplI GAGNNNNNCTC 2 cut(s) 427, 459
BpuMI CCSGG 3 cut(s) 129, 140, 255
BsaJI CCNNGG 7 cut(s) 127, 228, 254, 363, 400, 489, 535
BsaWI WCCGGW 3 cut(s) 10, 113, 202
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse118I RCCGGY 4 cut(s) 113, 185, 202, 506
BseDI CCNNGG 7 cut(s) 127, 228, 254, 363, 400, 489, 535
BseGI GGATG 2 cut(s) 96, 610
BseLI CCNNNNNNNGG 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 184
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 2 cut(s) 228, 838
BsgI GTGCAG 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 491
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 2 cut(s) 138, 399
BshTI ACCGGT 2 cut(s) 113, 202
BsiEI CGRYCG 1 cut(s) 139
BsiSI CCGG 8 cut(s) 11, 114, 129, 139, 186, 203, 254, 507
BslFI GGGAC 2 cut(s) 652, 710
BslI CCNNNNNNNGG 1 cut(s) 134
BsmFI GGGAC 2 cut(s) 652, 710
BsnI GGCC 2 cut(s) 138, 399
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 1 cut(s) 673
Bsp19I CCATGG 3 cut(s) 228, 400, 535
BspACI CCGC 3 cut(s) 215, 489, 491
BspANI GGCC 2 cut(s) 138, 399
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 2 cut(s) 97, 361
BspPI GGATC 1 cut(s) 681
BspTI CTTAAG 1 cut(s) 602
BsrFI RCCGGY 4 cut(s) 113, 185, 202, 506
BssAI RCCGGY 4 cut(s) 113, 185, 202, 506
BssECI CCNNGG 7 cut(s) 127, 228, 254, 363, 400, 489, 535
BssMI GATC 1 cut(s) 673
BssT1I CCWWGG 4 cut(s) 228, 363, 400, 535
Bst4CI ACNGT 3 cut(s) 44, 62, 760
Bst6I CTCTTC 1 cut(s) 170
BstAFI CTTAAG 1 cut(s) 602
BstC8I GCNNGC 1 cut(s) 305
BstDSI CCRYGG 4 cut(s) 228, 400, 489, 535
BstF5I GGATG 2 cut(s) 96, 610
BstFNI CGCG 1 cut(s) 491
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 1 cut(s) 676
BstMBI GATC 1 cut(s) 673
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 2 cut(s) 309, 607
BstSCI CCNGG 3 cut(s) 127, 138, 253
BstSFI CTRYAG 1 cut(s) 759
BstUI CGCG 1 cut(s) 491
BstV1I GCAGC 2 cut(s) 228, 838
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
BstZI CGGCCG 1 cut(s) 136
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 2 cut(s) 138, 399
BtgI CCRYGG 4 cut(s) 228, 400, 489, 535
BtsCI GGATG 2 cut(s) 96, 610
BtsI GCAGTG 1 cut(s) 640
BtsIMutI CAGTG 1 cut(s) 640
Cac8I GCNNGC 1 cut(s) 305
CfoI GCGC 1 cut(s) 488
Cfr10I RCCGGY 4 cut(s) 113, 185, 202, 506
Cfr13I GGNCC 2 cut(s) 8, 404
Cfr42I CCGCGG 1 cut(s) 492
CseI GACGC 3 cut(s) 163, 341, 501
Csp6I GTAC 1 cut(s) 815
CspAI ACCGGT 2 cut(s) 113, 202
CviAII CATG 5 cut(s) 38, 229, 401, 536, 589
CviQI GTAC 1 cut(s) 815
DpnI GATC 1 cut(s) 675
DpnII GATC 1 cut(s) 673
EaeI YGGCCR 2 cut(s) 136, 397
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 4 cut(s) 228, 363, 400, 535
Eco24I GRGCYC 1 cut(s) 98
Eco47I GGWCC 2 cut(s) 8, 404
Eco52I CGGCCG 1 cut(s) 136
EcoT14I CCWWGG 4 cut(s) 228, 363, 400, 535
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 4 cut(s) 228, 363, 400, 535
FaeI CATG 5 cut(s) 41, 232, 404, 539, 592
FaqI GGGAC 2 cut(s) 652, 710
FatI CATG 5 cut(s) 37, 228, 400, 535, 588
FauNDI CATATG 1 cut(s) 417
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 4 cut(s) 216, 242, 489, 852
FokI GGATG 2 cut(s) 103, 597
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 4 cut(s) 216, 242, 489, 852
FspBI CTAG 3 cut(s) 408, 702, 713
GlaI GCGC 1 cut(s) 487
GluI GCNGC 4 cut(s) 216, 242, 489, 852
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 2 cut(s) 138, 399
HapII CCGG 8 cut(s) 11, 114, 129, 139, 186, 203, 254, 507
HgaI GACGC 3 cut(s) 163, 341, 501
HhaI GCGC 1 cut(s) 488
Hin1II CATG 5 cut(s) 41, 232, 404, 539, 592
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 2 cut(s) 183, 756
HindII GTYRAC 2 cut(s) 183, 756
HinfI GANTC 4 cut(s) 250, 440, 563, 698
HpaII CCGG 8 cut(s) 11, 114, 129, 139, 186, 203, 254, 507
HphI GGTGA 5 cut(s) 103, 128, 192, 445, 551
Hpy166II GTNNAC 4 cut(s) 35, 183, 756, 763
Hpy188I TCNGA 1 cut(s) 283
Hpy188III TCNNGA 5 cut(s) 68, 268, 329, 671, 702
Hpy8I GTNNAC 4 cut(s) 35, 183, 756, 763
Hpy99I CGWCG 4 cut(s) 157, 199, 214, 223
HpyAV CCTTC 5 cut(s) 21, 424, 448, 568, 722
HpyCH4III ACNGT 3 cut(s) 44, 62, 760
HpyCH4IV ACGT 1 cut(s) 653
HpyCH4V TGCA 6 cut(s) 244, 610, 633, 686, 746, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 309, 607
HpySE526I ACGT 1 cut(s) 653
Hsp92II CATG 5 cut(s) 41, 232, 404, 539, 592
HspAI GCGC 1 cut(s) 486
KspI CCGCGG 1 cut(s) 492
Kzo9I GATC 1 cut(s) 673
LmnI GCTCC 4 cut(s) 101, 270, 365, 598
Lsp1109I GCAGC 2 cut(s) 228, 838
LweI GCATC 1 cut(s) 619
MaeI CTAG 3 cut(s) 408, 702, 713
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 4 cut(s) 116, 198, 649, 785
MalI GATC 1 cut(s) 675
MboI GATC 1 cut(s) 673
MboII GAAGA 5 cut(s) 138, 187, 380, 439, 770
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 1 cut(s) 98
MlsI TGGCCA 1 cut(s) 399
MluCI AATT 3 cut(s) 352, 542, 802
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 8 cut(s) 13, 113, 162, 165, 171, 252, 429, 624
Mox20I TGGCCA 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 275
MscI TGGCCA 1 cut(s) 399
MseI TTAA 5 cut(s) 582, 603, 767, 801, 842
MslI CAYNNNNRTG 3 cut(s) 42, 233, 507
Msp20I TGGCCA 1 cut(s) 399
MspA1I CMGCKG 1 cut(s) 491
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 8 cut(s) 11, 114, 129, 139, 186, 203, 254, 507
MspR9I CCNGG 3 cut(s) 129, 140, 255
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 2 cut(s) 309, 607
NciI CCSGG 3 cut(s) 129, 140, 255
NcoI CCATGG 3 cut(s) 228, 400, 535
NdeI CATATG 1 cut(s) 417
NdeII GATC 1 cut(s) 673
NlaIII CATG 5 cut(s) 41, 232, 404, 539, 592
NlaIV GGNNCC 2 cut(s) 97, 361
NmuCI GTSAC 2 cut(s) 116, 198
OliI CACNNNNGTG 1 cut(s) 507
PcsI WCGNNNNNNNCGW 2 cut(s) 215, 218
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 3 cut(s) 250, 440, 563
PflFI GACNNNGTC 1 cut(s) 74
PinAI ACCGGT 2 cut(s) 113, 202
PkrI GCNGC 4 cut(s) 217, 243, 490, 853
PleI GAGTC 1 cut(s) 692
PpsI GAGTC 1 cut(s) 692
PspN4I GGNNCC 2 cut(s) 97, 361
PspPI GGNCC 2 cut(s) 8, 404
PsuI RGATCY 1 cut(s) 673
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 1 cut(s) 816
RsaNI GTAC 1 cut(s) 815
RseI CAYNNNNRTG 3 cut(s) 42, 233, 507
SacII CCGCGG 1 cut(s) 492
SaqAI TTAA 5 cut(s) 582, 603, 767, 801, 842
SatI GCNGC 4 cut(s) 216, 242, 489, 852
Sau3AI GATC 1 cut(s) 673
Sau96I GGNCC 2 cut(s) 8, 404
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 3 cut(s) 129, 140, 255
SduI GDGCHC 1 cut(s) 98
SfaNI GCATC 1 cut(s) 619
SfcI CTRYAG 1 cut(s) 759
Sfr303I CCGCGG 1 cut(s) 492
SgrAI CRCCGGYG 2 cut(s) 113, 506
SgrBI CCGCGG 1 cut(s) 492
SinI GGWCC 2 cut(s) 8, 404
SmiMI CAYNNNNRTG 3 cut(s) 42, 233, 507
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
Sse9I AATT 3 cut(s) 352, 542, 802
SsiI CCGC 3 cut(s) 215, 489, 491
SspI AATATT 2 cut(s) 378, 691
SspMI CTAG 3 cut(s) 408, 702, 713
StyD4I CCNGG 3 cut(s) 127, 138, 253
StyI CCWWGG 4 cut(s) 228, 363, 400, 535
TaaI ACNGT 3 cut(s) 44, 62, 760
TaiI ACGT 1 cut(s) 656
TasI AATT 3 cut(s) 352, 542, 802
TatI WGTACW 1 cut(s) 814
TauI GCSGC 2 cut(s) 218, 491
TfiI GAWTC 3 cut(s) 250, 440, 563
Tru1I TTAA 5 cut(s) 582, 603, 767, 801, 842
Tru9I TTAA 5 cut(s) 582, 603, 767, 801, 842
TscAI CASTG 1 cut(s) 640
TseFI GTSAC 2 cut(s) 116, 198
TseI GCWGC 2 cut(s) 241, 851
Tsp45I GTSAC 2 cut(s) 116, 198
TspDTI ATGAA 1 cut(s) 741
TspRI CASTG 1 cut(s) 640
Tth111I GACNNNGTC 1 cut(s) 74
Vha464I CTTAAG 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 8, 404
XapI RAATTY 2 cut(s) 352, 542
XbaI TCTAGA 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 407
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 3 cut(s) 408, 702, 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.