Rw4G013310

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011554
Physical Location & Seq
Reverse (-)
31133928 .. 31139086
5159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013310.1

Sequence Viewer

Length: 1233 bp
ATGGTAGGTCCGGTGGGGGAGGCAAGAAGGGGTGGATATGACTGTGTGGTTGGGTTGGACTGTTTTCTGGACATCGTCAGGGCTTTAGGGATGGGCTCGTGTCTCTCCATCACCGGTGACAACTCCGCCGGCTACGGCCGGGTGTCTTCCCCGGCGCAGGGCAACAAGAGGAGGAAGAGGTTGACCGGCAACTCGTCGTCACCGGTTTCGTCGGCGGCGGCGAGTTCCATGGAGATGTGGCTGCACAGGATTCCGGGGAGGTTGTTCCTGAATGGCTTCTCTGATGTGGCTTCCCTGTTCAGCAAGCAAGGCAAGAAGGGTGTCAATCAGGACGCTATGATTGCTTGGGAGAATTTCGGCTCCAAGGAAGATACAATATTTTGTGGTGTTTTTGATGGGCATGGTCCTTGTGGTCATATGGTGGCGAAGAAGGTGAGGGATTCTCTACCTTTGAAGGTCAGCTCACAATGGGTGTTGAACACCAGCGCCGCGGATACTAACCACGCCGGTGCATCTGTGGAGACCAAGGACAGCCATGGTGAATTTTTCGCCACATTGAAAGATTCGTTTTTGAAGGCTTTTAAGGTCATGGACAAGGAGCTTAAGTTGCATCCTCGTATTGATTGCTATTGCAGTGGGACAACTGCAGTTACGTTGGTCAAGCAGGGTCAGGATCTTGTTATTGCAAATATTGGGGACTCTAGAGCTGTGCTAGGCACAAGAGATGAAGGGGGTTCTCTTGTTGCAGTTCAGTTGACCGTAGACTTAAAACCAAATCTTCCAAGGGAAGCCGAGAGGATTACGTTATGTAAAGGTCGAGTATTTGCTCTCCACAACGAGCCTGATGTCACTCGAGTTTGGTTGCCCAACAGTAATTCACCTGGGCTTGCTATGGCCCGGGCTTTTGGAGATTTCTGCCTAAAGGATTATGGTTTAATCTCTGTCCCTGATGTTTCTTATCGTCGCCTCACTGAGAAGGATGAATTTGTAGTACTGGCTACAGATGGGATCTGGGATGTTCTCTCTAACAAAGAAGTTGTGGATATAGTGGCAGCAACCCCACGACCCTCTGCTGCTCGAGCCCTGGTGGAGTCAGCCAATCGAGCTTGGAGATTTAAACACCCATATGCCAAGGTTGATGATTGTGCCGTTGTTTGCCTCTTCCTCACTTCCGGTGCTTCAGACAATACTTCCCTTTCCTCACCAGAGGAGAAGAAAGTGAACTCAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

410

Amino Acids

44.22

Weight (kDa)

8.23

Isoelectric Point (pI)

27.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 103 - 353 4e-42 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 762
AccII CGCG 1 cut(s) 491
AciI CCGC 5 cut(s) 126, 215, 218, 489, 491
AclWI GGATC 2 cut(s) 681, 1016
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 3 cut(s) 352, 542, 983
AcuI CTGAAG 1 cut(s) 1164
AfaI GTAC 1 cut(s) 993
AfiI CCNNNNNNNGG 2 cut(s) 157, 158
AflII CTTAAG 1 cut(s) 602
AgeI ACCGGT 2 cut(s) 113, 202
AgsI TTSAA 4 cut(s) 454, 478, 559, 574
AjnI CCWGG 2 cut(s) 880, 1083
AleI CACNNNNGTG 1 cut(s) 507
AluBI AGCT 4 cut(s) 462, 601, 707, 1106
AluI AGCT 4 cut(s) 462, 601, 707, 1106
Alw26I GTCTC 2 cut(s) 107, 515
AlwI GGATC 2 cut(s) 681, 1016
Ama87I CYCGRG 3 cut(s) 852, 897, 1077
AoxI GGCC 2 cut(s) 136, 894
ApeKI GCWGC 3 cut(s) 241, 1052, 1073
ApoI RAATTY 3 cut(s) 352, 542, 983
AsiGI ACCGGT 2 cut(s) 113, 202
Asp700I GAANNNNTTC 1 cut(s) 275
AspLEI GCGC 2 cut(s) 157, 488
AspS9I GGNCC 3 cut(s) 8, 404, 895
AsuC2I CCSGG 5 cut(s) 140, 152, 255, 898, 899
AsuHPI GGTGA 7 cut(s) 103, 128, 192, 445, 551, 870, 1194
AvaI CYCGRG 3 cut(s) 852, 897, 1077
AvaII GGWCC 2 cut(s) 8, 404
BanII GRGCYC 2 cut(s) 98, 1084
BauI CACGAG 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 138
BbvI GCAGC 3 cut(s) 228, 1060, 1064
BccI CCATC 4 cut(s) 85, 116, 389, 998
BceAI ACGGC 2 cut(s) 151, 1133
BciT130I CCWGG 2 cut(s) 882, 1085
BciVI GTATCC 1 cut(s) 487
BcnI CCSGG 5 cut(s) 140, 152, 255, 898, 899
BcoDI GTCTC 2 cut(s) 107, 515
BfaI CTAG 2 cut(s) 702, 713
BfmI CTRYAG 2 cut(s) 645, 999
BfoI RGCGCY 1 cut(s) 489
BfrI CTTAAG 1 cut(s) 602
BfuI GTATCC 1 cut(s) 487
BisI GCNGC 6 cut(s) 216, 219, 242, 489, 1053, 1074
BlsI GCNGC 6 cut(s) 217, 220, 243, 490, 1054, 1075
BmcAI AGTACT 1 cut(s) 993
Bme1390I CCNGG 7 cut(s) 140, 152, 255, 882, 898, 899, 1085
Bme18I GGWCC 2 cut(s) 8, 404
BmeT110I CYCGRG 3 cut(s) 852, 897, 1077
BmgT120I GGNCC 3 cut(s) 8, 404, 895
BmiI GGNNCC 1 cut(s) 361
BmrFI CCNGG 7 cut(s) 140, 152, 255, 882, 898, 899, 1085
BmsI GCATC 2 cut(s) 521, 619
BpiI GAAGAC 1 cut(s) 138
BplI GAGNNNNNCTC 2 cut(s) 427, 459
BpuMI CCSGG 5 cut(s) 140, 152, 255, 898, 899
BsaI GGTCTC 1 cut(s) 515
BsaWI WCCGGW 4 cut(s) 10, 113, 202, 1172
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 158
Bse118I RCCGGY 5 cut(s) 113, 128, 185, 202, 506
Bse1I ACTGG 1 cut(s) 999
BseBI CCWGG 2 cut(s) 882, 1085
BseGI GGATG 4 cut(s) 96, 610, 985, 1021
BseLI CCNNNNNNNGG 2 cut(s) 157, 158
BseMII CTCAG 1 cut(s) 963
BseNI ACTGG 1 cut(s) 999
BseRI GAGGAG 2 cut(s) 184, 1223
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 3 cut(s) 228, 1060, 1064
BsgI GTGCAG 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 491
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 2 cut(s) 138, 896
BshTI ACCGGT 2 cut(s) 113, 202
BsiEI CGRYCG 1 cut(s) 139
BsiHKCI CYCGRG 3 cut(s) 852, 897, 1077
BslFI GGGAC 3 cut(s) 652, 710, 929
BslI CCNNNNNNNGG 2 cut(s) 157, 158
BsmAI GTCTC 2 cut(s) 107, 515
BsmFI GGGAC 3 cut(s) 652, 710, 929
BsnI GGCC 2 cut(s) 138, 896
Bso31I GGTCTC 1 cut(s) 515
BsoBI CYCGRG 3 cut(s) 852, 897, 1077
Bsp1286I GDGCHC 2 cut(s) 98, 1084
Bsp143I GATC 2 cut(s) 673, 1008
Bsp19I CCATGG 2 cut(s) 228, 535
BspACI CCGC 5 cut(s) 126, 215, 218, 489, 491
BspANI GGCC 2 cut(s) 138, 896
BspCNI CTCAG 1 cut(s) 964
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 1 cut(s) 361
BspMAI CTGCAG 1 cut(s) 649
BspPI GGATC 2 cut(s) 681, 1016
BspTI CTTAAG 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 515
BsrFI RCCGGY 5 cut(s) 113, 128, 185, 202, 506
BsrI ACTGG 1 cut(s) 999
BssAI RCCGGY 5 cut(s) 113, 128, 185, 202, 506
BssMI GATC 2 cut(s) 673, 1008
BssSI CACGAG 1 cut(s) 97
BssT1I CCWWGG 6 cut(s) 228, 363, 525, 535, 782, 1131
Bst2BI CACGAG 1 cut(s) 97
Bst2UI CCWGG 2 cut(s) 882, 1085
Bst4CI ACNGT 4 cut(s) 44, 62, 760, 872
Bst6I CTCTTC 2 cut(s) 170, 1166
BstAFI CTTAAG 1 cut(s) 602
BstC8I GCNNGC 3 cut(s) 130, 305, 888
BstDEI CTNAG 1 cut(s) 972
BstDSI CCRYGG 3 cut(s) 228, 489, 535
BstF5I GGATG 4 cut(s) 96, 610, 985, 1021
BstFNI CGCG 1 cut(s) 491
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 2 cut(s) 157, 488
BstKTI GATC 2 cut(s) 676, 1011
BstMAI GTCTC 2 cut(s) 107, 515
BstMBI GATC 2 cut(s) 673, 1008
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 5 cut(s) 309, 341, 607, 1079, 1103
BstNI CCWGG 2 cut(s) 882, 1085
BstSCI CCNGG 7 cut(s) 138, 150, 253, 880, 896, 897, 1083
BstSFI CTRYAG 2 cut(s) 645, 999
BstUI CGCG 1 cut(s) 491
BstV1I GCAGC 3 cut(s) 228, 1060, 1064
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 2 cut(s) 673, 1008
BstYI RGATCY 2 cut(s) 673, 1008
BstZI CGGCCG 1 cut(s) 136
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 2 cut(s) 138, 896
BtgI CCRYGG 3 cut(s) 228, 489, 535
BtsCI GGATG 4 cut(s) 96, 610, 985, 1021
BtsI GCAGTG 1 cut(s) 640
BtsIMutI CAGTG 2 cut(s) 640, 969
Cac8I GCNNGC 3 cut(s) 130, 305, 888
CfoI GCGC 2 cut(s) 157, 488
Cfr10I RCCGGY 5 cut(s) 113, 128, 185, 202, 506
Cfr13I GGNCC 3 cut(s) 8, 404, 895
Cfr42I CCGCGG 1 cut(s) 492
Cfr9I CCCGGG 1 cut(s) 897
CseI GACGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 992
CspAI ACCGGT 2 cut(s) 113, 202
CviAII CATG 4 cut(s) 229, 401, 536, 589
CviQI GTAC 1 cut(s) 992
DdeI CTNAG 1 cut(s) 972
DpnI GATC 2 cut(s) 675, 1010
DpnII GATC 2 cut(s) 673, 1008
DraI TTTAAA 1 cut(s) 1117
EaeI YGGCCR 1 cut(s) 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 2 cut(s) 170, 1166
EarI CTCTTC 2 cut(s) 170, 1166
EciI GGCGGA 1 cut(s) 115
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 6 cut(s) 228, 363, 525, 535, 782, 1131
Eco24I GRGCYC 2 cut(s) 98, 1084
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 2 cut(s) 8, 404
Eco52I CGGCCG 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 1164
Eco88I CYCGRG 3 cut(s) 852, 897, 1077
EcoRII CCWGG 2 cut(s) 880, 1083
EcoT14I CCWWGG 6 cut(s) 228, 363, 525, 535, 782, 1131
EcoT38I GRGCYC 2 cut(s) 98, 1084
ErhI CCWWGG 6 cut(s) 228, 363, 525, 535, 782, 1131
FaeI CATG 4 cut(s) 232, 404, 539, 592
FaqI GGGAC 3 cut(s) 652, 710, 929
FatI CATG 4 cut(s) 228, 400, 535, 588
FauNDI CATATG 2 cut(s) 417, 1126
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 6 cut(s) 216, 219, 242, 489, 1053, 1074
FokI GGATG 4 cut(s) 103, 597, 992, 1028
FriOI GRGCYC 2 cut(s) 98, 1084
Fsp4HI GCNGC 6 cut(s) 216, 219, 242, 489, 1053, 1074
FspBI CTAG 2 cut(s) 702, 713
GlaI GCGC 2 cut(s) 156, 487
GluI GCNGC 6 cut(s) 216, 219, 242, 489, 1053, 1074
HaeII RGCGCY 1 cut(s) 489
HaeIII GGCC 2 cut(s) 138, 896
HgaI GACGC 1 cut(s) 341
HhaI GCGC 2 cut(s) 157, 488
Hin1II CATG 4 cut(s) 232, 404, 539, 592
Hin6I GCGC 2 cut(s) 155, 486
HinP1I GCGC 2 cut(s) 155, 486
HincII GTYRAC 2 cut(s) 183, 756
HindII GTYRAC 2 cut(s) 183, 756
HinfI GANTC 5 cut(s) 250, 440, 563, 698, 1091
HphI GGTGA 7 cut(s) 103, 128, 192, 445, 551, 870, 1194
Hpy166II GTNNAC 4 cut(s) 183, 756, 763, 1222
Hpy188I TCNGA 2 cut(s) 283, 1183
Hpy188III TCNNGA 5 cut(s) 68, 268, 329, 671, 702
Hpy8I GTNNAC 4 cut(s) 183, 756, 763, 1222
Hpy99I CGWCG 3 cut(s) 199, 214, 966
HpyAV CCTTC 7 cut(s) 21, 310, 424, 448, 568, 722, 970
HpyCH4III ACNGT 4 cut(s) 44, 62, 760, 872
HpyCH4IV ACGT 2 cut(s) 653, 803
HpyCH4V TGCA 7 cut(s) 244, 512, 610, 633, 647, 686, 746
HpyF10VI GCNNNNNNNGC 5 cut(s) 309, 341, 607, 1079, 1103
HpyF3I CTNAG 1 cut(s) 972
HpySE526I ACGT 2 cut(s) 653, 803
Hsp92II CATG 4 cut(s) 232, 404, 539, 592
HspAI GCGC 2 cut(s) 155, 486
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
KspI CCGCGG 1 cut(s) 492
Kzo9I GATC 2 cut(s) 673, 1008
LmnI GCTCC 2 cut(s) 365, 598
Lsp1109I GCAGC 3 cut(s) 228, 1060, 1064
LweI GCATC 2 cut(s) 521, 619
MaeI CTAG 2 cut(s) 702, 713
MaeII ACGT 2 cut(s) 653, 803
MaeIII GTNAC 4 cut(s) 116, 198, 649, 847
MalI GATC 2 cut(s) 675, 1010
MboI GATC 2 cut(s) 673, 1008
MboII GAAGA 7 cut(s) 138, 187, 380, 439, 770, 1153, 1225
MflI RGATCY 2 cut(s) 673, 1008
MhlI GDGCHC 2 cut(s) 98, 1084
MluCI AATT 4 cut(s) 352, 542, 874, 983
MlyI GAGTC 2 cut(s) 692, 1100
MmeI TCCRAC 1 cut(s) 36
MroNI GCCGGC 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 5 cut(s) 582, 603, 767, 935, 1116
MslI CAYNNNNRTG 3 cut(s) 233, 507, 1125
MspA1I CMGCKG 1 cut(s) 491
MspCI CTTAAG 1 cut(s) 602
MspR9I CCNGG 7 cut(s) 140, 152, 255, 882, 898, 899, 1085
MvaI CCWGG 2 cut(s) 882, 1085
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 5 cut(s) 309, 341, 607, 1079, 1103
NaeI GCCGGC 1 cut(s) 130
NciI CCSGG 5 cut(s) 140, 152, 255, 898, 899
NcoI CCATGG 2 cut(s) 228, 535
NdeI CATATG 2 cut(s) 417, 1126
NdeII GATC 2 cut(s) 673, 1008
NgoMIV GCCGGC 1 cut(s) 128
NlaIII CATG 4 cut(s) 232, 404, 539, 592
NlaIV GGNNCC 1 cut(s) 361
NmeAIII GCCGAG 1 cut(s) 817
NmuCI GTSAC 3 cut(s) 116, 198, 847
OliI CACNNNNGTG 1 cut(s) 507
PaeR7I CTCGAG 2 cut(s) 852, 1077
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PdiI GCCGGC 1 cut(s) 130
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 3 cut(s) 250, 440, 563
PflFI GACNNNGTC 1 cut(s) 74
PinAI ACCGGT 2 cut(s) 113, 202
PkrI GCNGC 6 cut(s) 217, 220, 243, 490, 1054, 1075
PleI GAGTC 2 cut(s) 692, 1099
PpsI GAGTC 2 cut(s) 692, 1099
Psp6I CCWGG 2 cut(s) 880, 1083
PspGI CCWGG 2 cut(s) 880, 1083
PspN4I GGNNCC 1 cut(s) 361
PspPI GGNCC 3 cut(s) 8, 404, 895
PspXI VCTCGAGB 2 cut(s) 852, 1077
PstI CTGCAG 1 cut(s) 649
PsuI RGATCY 2 cut(s) 673, 1008
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 1 cut(s) 993
RsaNI GTAC 1 cut(s) 992
RseI CAYNNNNRTG 3 cut(s) 233, 507, 1125
SacII CCGCGG 1 cut(s) 492
SaqAI TTAA 5 cut(s) 582, 603, 767, 935, 1116
SatI GCNGC 6 cut(s) 216, 219, 242, 489, 1053, 1074
Sau3AI GATC 2 cut(s) 673, 1008
Sau96I GGNCC 3 cut(s) 8, 404, 895
ScaI AGTACT 1 cut(s) 993
SchI GAGTC 2 cut(s) 692, 1100
ScrFI CCNGG 7 cut(s) 140, 152, 255, 882, 898, 899, 1085
SduI GDGCHC 2 cut(s) 98, 1084
SfaNI GCATC 2 cut(s) 521, 619
SfcI CTRYAG 2 cut(s) 645, 999
Sfr274I CTCGAG 2 cut(s) 852, 1077
Sfr303I CCGCGG 1 cut(s) 492
SgrAI CRCCGGYG 2 cut(s) 113, 506
SgrBI CCGCGG 1 cut(s) 492
SinI GGWCC 2 cut(s) 8, 404
SlaI CTCGAG 2 cut(s) 852, 1077
SmaI CCCGGG 1 cut(s) 899
SmiMI CAYNNNNRTG 3 cut(s) 233, 507, 1125
SmlI CTYRAG 3 cut(s) 602, 852, 1077
SmoI CTYRAG 3 cut(s) 602, 852, 1077
SrfI GCCCGGGC 1 cut(s) 899
Sse9I AATT 4 cut(s) 352, 542, 874, 983
SsiI CCGC 5 cut(s) 126, 215, 218, 489, 491
SspI AATATT 2 cut(s) 378, 691
SspMI CTAG 2 cut(s) 702, 713
StyD4I CCNGG 7 cut(s) 138, 150, 253, 880, 896, 897, 1083
StyI CCWWGG 6 cut(s) 228, 363, 525, 535, 782, 1131
TaaI ACNGT 4 cut(s) 44, 62, 760, 872
TaiI ACGT 2 cut(s) 656, 806
TaqI TCGA 4 cut(s) 817, 853, 1078, 1102
TasI AATT 4 cut(s) 352, 542, 874, 983
TatI WGTACW 1 cut(s) 991
TauI GCSGC 3 cut(s) 218, 221, 491
TfiI GAWTC 3 cut(s) 250, 440, 563
Tru1I TTAA 5 cut(s) 582, 603, 767, 935, 1116
Tru9I TTAA 5 cut(s) 582, 603, 767, 935, 1116
TscAI CASTG 2 cut(s) 640, 976
TseFI GTSAC 3 cut(s) 116, 198, 847
TseI GCWGC 3 cut(s) 241, 1052, 1073
Tsp45I GTSAC 3 cut(s) 116, 198, 847
TspDTI ATGAA 2 cut(s) 741, 996
TspMI CCCGGG 1 cut(s) 897
TspRI CASTG 2 cut(s) 640, 976
Tth111I GACNNNGTC 1 cut(s) 74
Vha464I CTTAAG 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 8, 404
XapI RAATTY 3 cut(s) 352, 542, 983
XbaI TCTAGA 1 cut(s) 701
XhoI CTCGAG 2 cut(s) 852, 1077
XmaI CCCGGG 1 cut(s) 897
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 2 cut(s) 702, 713
ZrmI AGTACT 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.