pycom04g03700

FAR1 DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004298
Physical Location & Seq
Reverse (-)
3303825 .. 3305751
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGGATGATTTGAATGGGACAGTCGAAACTTCTGCAGAAGGGGAATCAAACAATATTGAAGTTGGCCCATGTCAAGAACCATATGAGGGTATGCTTTTTGAATCAGAAGAAGCTGCCAAAGTATTCTATGATGATTATGCCAGGCGTGTTGGATTTGTGACCCGTGTTTTGTCATCCCGGAAGTCTGAGCGTGATGGGTCAATAATCTCTCGTGGACTTGGATGCAGAGGGATCTCTGAGAATCGTAGAAAAGTAATGCAGAAGGAGTTTGGAGAACAAGAAGCTTGTCCGGCCATGGTCTTGTTGAAACAAGAGATGCCTGGAAGATGGGTTATCAAGAAATTTTTGGGGGACCATAATCATCTGTTAGTGATTCAATCACCAAAGATCCGCAAAACACTTGATGAGAAGGATAAGAAAATCCAGGAGTTATCTGCAGAGCTACGAGTTAAGAAGCGGTTAAGTGCAGCATATAGAGATCAGCTACTCGCCTTTATCAAGGATATTGAAGACCACAACGCGCACCTTTCAACAAAAGTTCAATCGGTTTCTAACAAGATAAAAGAGCTTGAAGCTAAAACGGGTGCTTTAACGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

200

Amino Acids

22.57

Weight (kDa)

8.35

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 42 - 124 2e-17 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 521, 595
AciI CCGC 2 cut(s) 391, 457
AclWI GGATC 2 cut(s) 239, 382
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 9 cut(s) 13, 59, 101, 307, 377, 509, 531, 542, 572
AjnI CCWGG 3 cut(s) 140, 319, 423
AluBI AGCT 6 cut(s) 113, 284, 442, 484, 568, 575
AluI AGCT 6 cut(s) 113, 284, 442, 484, 568, 575
AlwI GGATC 2 cut(s) 239, 382
AoxI GGCC 2 cut(s) 64, 291
ApeKI GCWGC 2 cut(s) 113, 467
ApoI RAATTY 1 cut(s) 341
ArsI GACNNNNNNTTYG 2 cut(s) 151, 183
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 2 cut(s) 65, 352
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 1 cut(s) 372
AvaII GGWCC 1 cut(s) 352
BauI CACGAG 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 100, 479
BccI CCATC 2 cut(s) 188, 321
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 3 cut(s) 142, 321, 425
BcnI CCSGG 1 cut(s) 178
BfmI CTRYAG 2 cut(s) 33, 435
BisI GCNGC 2 cut(s) 114, 468
BlsI GCNGC 2 cut(s) 115, 469
Bme1390I CCNGG 4 cut(s) 142, 178, 321, 425
Bme18I GGWCC 1 cut(s) 352
BmgT120I GGNCC 2 cut(s) 65, 352
BmiI GGNNCC 1 cut(s) 353
BmrFI CCNGG 4 cut(s) 142, 178, 321, 425
BmsI GCATC 2 cut(s) 212, 306
BpiI GAAGAC 1 cut(s) 516
BpuMI CCSGG 1 cut(s) 178
BsaJI CCNNGG 1 cut(s) 294
Bsc4I CCNNNNNNNGG 1 cut(s) 86
BseBI CCWGG 3 cut(s) 142, 321, 425
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 3 cut(s) 10, 173, 227
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 2 cut(s) 177, 228
BseXI GCAGC 2 cut(s) 100, 479
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 2 cut(s) 521, 595
BshFI GGCC 2 cut(s) 66, 293
BsiSI CCGG 2 cut(s) 178, 290
BslFI GGGAC 2 cut(s) 31, 365
BslI CCNNNNNNNGG 1 cut(s) 86
BsmFI GGGAC 2 cut(s) 31, 365
BsnI GGCC 2 cut(s) 66, 293
Bsp143I GATC 3 cut(s) 231, 387, 478
Bsp19I CCATGG 1 cut(s) 294
BspACI CCGC 2 cut(s) 391, 457
BspANI GGCC 2 cut(s) 66, 293
BspCNI CTCAG 2 cut(s) 178, 229
BspFNI CGCG 2 cut(s) 521, 595
BspLI GGNNCC 1 cut(s) 353
BspMAI CTGCAG 2 cut(s) 37, 439
BspPI GGATC 2 cut(s) 239, 382
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 3 cut(s) 231, 387, 478
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 294
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 3 cut(s) 142, 321, 425
Bst4CI ACNGT 1 cut(s) 22
BstDEI CTNAG 2 cut(s) 186, 237
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 3 cut(s) 10, 173, 227
BstFNI CGCG 2 cut(s) 521, 595
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 3 cut(s) 234, 390, 481
BstMBI GATC 3 cut(s) 231, 387, 478
BstMWI GCNNNNNNNGC 1 cut(s) 290
BstNI CCWGG 3 cut(s) 142, 321, 425
BstSCI CCNGG 4 cut(s) 140, 176, 319, 423
BstSFI CTRYAG 2 cut(s) 33, 435
BstUI CGCG 2 cut(s) 521, 595
BstV1I GCAGC 2 cut(s) 100, 479
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 2 cut(s) 231, 387
BstYI RGATCY 2 cut(s) 231, 387
BsuRI GGCC 2 cut(s) 66, 293
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 3 cut(s) 10, 173, 227
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 2 cut(s) 65, 352
CviAII CATG 2 cut(s) 69, 295
CviJI RGCY 8 cut(s) 66, 113, 284, 293, 442, 484, 568, 575
CviKI_1 RGCY 8 cut(s) 66, 113, 284, 293, 442, 484, 568, 575
DdeI CTNAG 2 cut(s) 186, 237
DpnI GATC 3 cut(s) 233, 389, 480
DpnII GATC 3 cut(s) 231, 387, 478
EaeI YGGCCR 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 352
EcoRII CCWGG 3 cut(s) 140, 319, 423
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 2 cut(s) 72, 298
FaqI GGGAC 2 cut(s) 31, 365
FatI CATG 2 cut(s) 68, 294
FauNDI CATATG 1 cut(s) 82
Fnu4HI GCNGC 2 cut(s) 114, 468
FokI GGATG 3 cut(s) 17, 160, 234
Fsp4HI GCNGC 2 cut(s) 114, 468
GlaI GCGC 1 cut(s) 522
GluI GCNGC 2 cut(s) 114, 468
HaeIII GGCC 2 cut(s) 66, 293
HapII CCGG 2 cut(s) 178, 290
HhaI GCGC 1 cut(s) 523
Hin1II CATG 2 cut(s) 72, 298
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HindIII AAGCTT 1 cut(s) 282
HinfI GANTC 4 cut(s) 44, 101, 241, 373
HpaII CCGG 2 cut(s) 178, 290
HphI GGTGA 1 cut(s) 372
Hpy166II GTNNAC 1 cut(s) 215
Hpy188I TCNGA 3 cut(s) 106, 187, 238
Hpy188III TCNNGA 2 cut(s) 74, 337
Hpy8I GTNNAC 1 cut(s) 215
HpyAV CCTTC 3 cut(s) 32, 256, 403
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4V TGCA 5 cut(s) 35, 225, 259, 437, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 290
HpyF3I CTNAG 2 cut(s) 186, 237
Hsp92II CATG 2 cut(s) 72, 298
HspAI GCGC 1 cut(s) 521
Kzo9I GATC 3 cut(s) 231, 387, 478
LpnPI CCDG 8 cut(s) 127, 154, 191, 303, 306, 333, 410, 437
Lsp1109I GCAGC 2 cut(s) 100, 479
LweI GCATC 2 cut(s) 212, 306
MaeIII GTNAC 1 cut(s) 157
MalI GATC 3 cut(s) 233, 389, 480
MboI GATC 3 cut(s) 231, 387, 478
MboII GAAGA 3 cut(s) 119, 336, 521
MflI RGATCY 2 cut(s) 231, 387
MluCI AATT 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 2 cut(s) 79, 221
MseI TTAA 3 cut(s) 450, 461, 590
MspI CCGG 2 cut(s) 178, 290
MspR9I CCNGG 4 cut(s) 142, 178, 321, 425
MvaI CCWGG 3 cut(s) 142, 321, 425
MvnI CGCG 2 cut(s) 521, 595
MwoI GCNNNNNNNGC 1 cut(s) 290
NciI CCSGG 1 cut(s) 178
NcoI CCATGG 1 cut(s) 294
NdeI CATATG 1 cut(s) 82
NdeII GATC 3 cut(s) 231, 387, 478
NlaIII CATG 2 cut(s) 72, 298
NlaIV GGNNCC 1 cut(s) 353
NmuCI GTSAC 1 cut(s) 157
PfeI GAWTC 4 cut(s) 44, 101, 241, 373
PfoI TCCNGGA 2 cut(s) 176, 423
PkrI GCNGC 2 cut(s) 115, 469
Psp6I CCWGG 3 cut(s) 140, 319, 423
PspGI CCWGG 3 cut(s) 140, 319, 423
PspN4I GGNNCC 1 cut(s) 353
PspPI GGNCC 2 cut(s) 65, 352
PstI CTGCAG 2 cut(s) 37, 439
PsuI RGATCY 2 cut(s) 231, 387
SaqAI TTAA 3 cut(s) 450, 461, 590
SatI GCNGC 2 cut(s) 114, 468
Sau3AI GATC 3 cut(s) 231, 387, 478
Sau96I GGNCC 2 cut(s) 65, 352
ScrFI CCNGG 4 cut(s) 142, 178, 321, 425
SetI ASST 7 cut(s) 115, 286, 444, 486, 528, 570, 577
SfaNI GCATC 2 cut(s) 212, 306
SfcI CTRYAG 2 cut(s) 33, 435
SinI GGWCC 1 cut(s) 352
Sse9I AATT 1 cut(s) 341
SsiI CCGC 2 cut(s) 391, 457
SspI AATATT 1 cut(s) 55
StyD4I CCNGG 4 cut(s) 140, 176, 319, 423
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 1 cut(s) 22
TaqI TCGA 1 cut(s) 24
TasI AATT 1 cut(s) 341
TfiI GAWTC 4 cut(s) 44, 101, 241, 373
Tru1I TTAA 3 cut(s) 450, 461, 590
Tru9I TTAA 3 cut(s) 450, 461, 590
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 2 cut(s) 113, 467
Tsp45I GTSAC 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.