pycom04g06700

Protein TPX2-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005322
Physical Location & Seq
Reverse (-)
6903812 .. 6908205
4394 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1302 bp
ATGGAAATGGAGGACGATATGGAGGTTGAGGAAGTGTTTATGGCACAGGAAGTGGACGTTGACTACGAGTTCGATGCAGCTTGGTACTTTGACTTCACTAGGGAGGAGACGCCGGCTGAGGCTCGCCGCGCCGAGCTCTGGTTCGAGTCCGCCAAGAGCTACCTGCCGTCTCGACTGACATTCAACAGCCACCTAACCGGTGATAAGCTTAAAGGTCAGAGTAATGCTTCTGTAAAGCCATCGTTCCCCAGGAGCTCAACTTTGATGAAGCCCACAGCAAGTCAGCTCGCTAAGCAAAATCTGAAGCCTCAAAATGGTGGTTCCAGATTCCAAATGCTGCATGTTCAAAACAATGAGAAGAGCTTGTGTAGTTCTTCTGGGGTTGAAAGTCAAGCTCCCAAGAGACAGAAGCTAGATGGAGGGCACTTGCGTAAGGTTACTGATGCAAAGCAGCAAACTAATTTTGTCCACAAGGTACCTAAAAAGGATGAAACTTTTGACAAAACTACTGCACACTCTAAGTTGAGACTTACCATTCCAAGAGAGCCTGACTTTGAAACGGCACATAGGGCACACAGGATTAGGCCTAACAATGGATCTATGTTAGAACAAGTGACATCAACTCATCGAAAATTCAAAGCTCGTCCATTGAACCGAAAAATTTTCGAGGCTCCTTCATTGCCACTTCCAACAAGGAGCACTCCAAAGTTGCCGGAATTTCAAGAATTTCACCTCAAGACAATGGAAAGGGCTATGCAAAATGCATCGGCTGTTTCATCATCCTCACATCATCTCAATGGTCACGATAAGGGTTTGGAAAAACCTAGCATTTCTACCGTTGCGGAAAACAGAAAAGGAGAATCCAGAAGACCAAATACAGTGGAATGTCCTAAGCAGGATGGAAATGCAGGAATGCAAACATTTAAAGCTCGTCCTCTTAACAAAAAGATATTTTCTAGCAAAGGGAATATTGGTGTTTTCTGGAATAGCAAGCGAGAAACCACTGTACCAATGGAATTTAATTTTCAGACAGACAGAAGAGTACAGCAATTTCCGCCAACCGAACTTTTTAGTAAGCTCTCTTTAACTACTGAGCGCCAGCCAAATAATGGATCACAGGTGAAACTATCCCAGCCTACATCTTTGTTTACGAAGGACTCAAAAGAAAATAGATCGACTGTTTTCCAACAAGAACATAAGATAAAAGAAAAACCTTCTCTGTTTGGTGGAAAGCAGGCTCAAGACAGGTGCACAACCGATGTTGATAAGAAATTGAGGATTAGTGGGTTGGGCGTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2_importin

Protein Analysis

434

Amino Acids

49.28

Weight (kDa)

9.73

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2_importin PF12214 175 - 342 8.8e-47 Cell cycle regulated microtubule associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 171
Acc65I GGTACC 1 cut(s) 475
AccB1I GGYRCC 1 cut(s) 475
AccB7I CCANNNNNTGG 1 cut(s) 1109
AccII CGCG 1 cut(s) 129
AciI CCGC 4 cut(s) 127, 150, 842, 1055
AclWI GGATC 2 cut(s) 604, 1120
AcsI RAATTY 5 cut(s) 632, 660, 716, 725, 1016
AcuI CTGAAG 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 110
AfaI GTAC 4 cut(s) 86, 477, 1008, 1044
AfiI CCNNNNNNNGG 4 cut(s) 138, 314, 593, 1109
AgeI ACCGGT 1 cut(s) 197
AgsI TTSAA 7 cut(s) 184, 347, 386, 557, 637, 652, 722
AjnI CCWGG 1 cut(s) 248
Alw21I GWGCWC 4 cut(s) 138, 257, 701, 1253
Alw26I GTCTC 4 cut(s) 101, 174, 397, 520
Alw44I GTGCAC 1 cut(s) 1249
AlwI GGATC 2 cut(s) 604, 1120
AoxI GGCC 1 cut(s) 584
ApaLI GTGCAC 1 cut(s) 1249
ApeKI GCWGC 3 cut(s) 77, 337, 451
ApoI RAATTY 5 cut(s) 632, 660, 716, 725, 1016
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AsiGI ACCGGT 1 cut(s) 197
Asp718I GGTACC 1 cut(s) 475
AspLEI GCGC 2 cut(s) 131, 1098
AsuHPI GGTGA 3 cut(s) 212, 722, 1132
BaeGI GKGCMC 3 cut(s) 426, 574, 1253
BaeI ACNNNNGTAYC 2 cut(s) 990, 1023
BanI GGYRCC 1 cut(s) 475
BanII GRGCYC 2 cut(s) 138, 257
BarI GAAGNNNNNNTAC 4 cut(s) 77, 109, 859, 891
BbsI GAAGAC 1 cut(s) 874
Bbv12I GWGCWC 4 cut(s) 138, 257, 701, 1253
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 3 cut(s) 89, 324, 463
BccI CCATC 3 cut(s) 247, 410, 893
BceAI ACGGC 2 cut(s) 151, 576
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BciT130I CCWGG 1 cut(s) 250
BcoDI GTCTC 4 cut(s) 101, 174, 397, 520
BfaI CTAG 4 cut(s) 99, 413, 825, 957
BfoI RGCGCY 1 cut(s) 1099
BfuAI ACCTGC 1 cut(s) 171
BisI GCNGC 4 cut(s) 78, 127, 338, 452
BlpI GCTNAGC 1 cut(s) 291
BlsI GCNGC 4 cut(s) 79, 128, 339, 453
Bme1390I CCNGG 1 cut(s) 250
BmiI GGNNCC 3 cut(s) 322, 477, 672
BmrFI CCNGG 1 cut(s) 250
BmsI GCATC 3 cut(s) 64, 433, 773
BpiI GAAGAC 1 cut(s) 874
Bpu10I CCTNAGC 2 cut(s) 117, 891
Bpu1102I GCTNAGC 1 cut(s) 291
BpuEI CTTGAG 2 cut(s) 719, 1224
BsaHI GRCGYC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 248
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 314, 593, 1109
Bse118I RCCGGY 2 cut(s) 112, 197
Bse3DI GCAATG 1 cut(s) 677
BseBI CCWGG 1 cut(s) 250
BseDI CCNNGG 1 cut(s) 248
BseGI GGATG 3 cut(s) 493, 779, 904
BseLI CCNNNNNNNGG 4 cut(s) 138, 314, 593, 1109
BseMI GCAATG 1 cut(s) 677
BseMII CTCAG 2 cut(s) 108, 1083
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 3 cut(s) 426, 574, 1253
BseXI GCAGC 3 cut(s) 89, 324, 463
BseYI CCCAGC 1 cut(s) 1131
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 1 cut(s) 586
BshNI GGYRCC 1 cut(s) 475
BshTI ACCGGT 1 cut(s) 197
BsiHKAI GWGCWC 4 cut(s) 138, 257, 701, 1253
BsiSI CCGG 3 cut(s) 113, 198, 713
BslI CCNNNNNNNGG 4 cut(s) 138, 314, 593, 1109
BsmAI GTCTC 4 cut(s) 101, 174, 397, 520
BsmBI CGTCTC 2 cut(s) 101, 174
BsmI GAATGC 1 cut(s) 918
BsnI GGCC 1 cut(s) 586
Bsp1286I GDGCHC 6 cut(s) 138, 257, 426, 574, 701, 1253
Bsp143I GATC 3 cut(s) 596, 1112, 1172
Bsp1720I GCTNAGC 1 cut(s) 291
BspACI CCGC 4 cut(s) 127, 150, 842, 1055
BspANI GGCC 1 cut(s) 586
BspCNI CTCAG 2 cut(s) 109, 1084
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 3 cut(s) 322, 477, 672
BspMI ACCTGC 1 cut(s) 171
BspPI GGATC 2 cut(s) 604, 1120
BspQI GCTCTTC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 677
BsrFI RCCGGY 2 cut(s) 112, 197
BssAI RCCGGY 2 cut(s) 112, 197
BssECI CCNNGG 1 cut(s) 248
BssMI GATC 3 cut(s) 596, 1112, 1172
BssNI GRCGYC 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 250
Bst4CI ACNGT 4 cut(s) 838, 880, 1006, 1180
Bst6I CTCTTC 2 cut(s) 353, 1033
BstACI GRCGYC 1 cut(s) 110
BstC8I GCNNGC 6 cut(s) 114, 124, 288, 992, 1100, 1236
BstDEI CTNAG 5 cut(s) 117, 291, 519, 891, 1092
BstF5I GGATG 3 cut(s) 493, 779, 904
BstFNI CGCG 1 cut(s) 129
BstH2I RGCGCY 1 cut(s) 1099
BstHHI GCGC 2 cut(s) 131, 1098
BstKTI GATC 3 cut(s) 599, 1115, 1175
BstMAI GTCTC 4 cut(s) 101, 174, 397, 520
BstMBI GATC 3 cut(s) 596, 1112, 1172
BstMWI GCNNNNNNNGC 4 cut(s) 128, 292, 569, 1054
BstNI CCWGG 1 cut(s) 250
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 248
BstSLI GKGCMC 3 cut(s) 426, 574, 1253
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 89, 324, 463
BstV2I GAAGAC 1 cut(s) 874
BstX2I RGATCY 1 cut(s) 596
BstYI RGATCY 1 cut(s) 596
BsuRI GGCC 1 cut(s) 586
BtsCI GGATG 3 cut(s) 493, 779, 904
BtsIMutI CAGTG 2 cut(s) 885, 1002
BveI ACCTGC 1 cut(s) 171
Cac8I GCNNGC 6 cut(s) 114, 124, 288, 992, 1100, 1236
CfoI GCGC 2 cut(s) 131, 1098
Cfr10I RCCGGY 2 cut(s) 112, 197
CseI GACGC 1 cut(s) 118
Csp6I GTAC 4 cut(s) 85, 476, 1007, 1043
CspAI ACCGGT 1 cut(s) 197
CspCI CAANNNNNGTGG 2 cut(s) 861, 896
CviAII CATG 1 cut(s) 341
CviQI GTAC 4 cut(s) 85, 476, 1007, 1043
DdeI CTNAG 5 cut(s) 117, 291, 519, 891, 1092
DpnI GATC 3 cut(s) 598, 1114, 1174
DpnII GATC 3 cut(s) 596, 1112, 1172
DraI TTTAAA 1 cut(s) 925
Eam1104I CTCTTC 2 cut(s) 353, 1033
EarI CTCTTC 2 cut(s) 353, 1033
EciI GGCGGA 2 cut(s) 139, 1044
Ecl136II GAGCTC 2 cut(s) 136, 255
Eco147I AGGCCT 1 cut(s) 586
Eco24I GRGCYC 2 cut(s) 138, 257
Eco53kI GAGCTC 2 cut(s) 136, 255
Eco57I CTGAAG 1 cut(s) 323
EcoICRI GAGCTC 2 cut(s) 136, 255
EcoRII CCWGG 1 cut(s) 248
EcoT22I ATGCAT 1 cut(s) 766
EcoT38I GRGCYC 2 cut(s) 138, 257
Esp3I CGTCTC 2 cut(s) 101, 174
FaeI CATG 1 cut(s) 344
FaiI YATR 7 cut(s) 20, 41, 342, 567, 602, 755, 1197
FatI CATG 1 cut(s) 340
Fnu4HI GCNGC 4 cut(s) 78, 127, 338, 452
FokI GGATG 3 cut(s) 500, 766, 911
FriOI GRGCYC 2 cut(s) 138, 257
Fsp4HI GCNGC 4 cut(s) 78, 127, 338, 452
FspBI CTAG 4 cut(s) 99, 413, 825, 957
GlaI GCGC 2 cut(s) 130, 1097
GluI GCNGC 4 cut(s) 78, 127, 338, 452
GsaI CCCAGC 1 cut(s) 1135
HaeII RGCGCY 1 cut(s) 1099
HaeIII GGCC 1 cut(s) 586
HapII CCGG 3 cut(s) 113, 198, 713
HgaI GACGC 1 cut(s) 118
HhaI GCGC 2 cut(s) 131, 1098
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 2 cut(s) 129, 1096
HinP1I GCGC 2 cut(s) 129, 1096
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 4 cut(s) 146, 327, 860, 1157
HpaII CCGG 3 cut(s) 113, 198, 713
HphI GGTGA 3 cut(s) 212, 722, 1132
Hpy166II GTNNAC 5 cut(s) 55, 61, 469, 1149, 1251
Hpy188I TCNGA 3 cut(s) 219, 303, 1029
Hpy188III TCNNGA 8 cut(s) 171, 324, 722, 736, 803, 864, 982, 1241
Hpy8I GTNNAC 5 cut(s) 55, 61, 469, 1149, 1251
HpyAV CCTTC 3 cut(s) 684, 1147, 1224
HpyCH4III ACNGT 4 cut(s) 838, 880, 1006, 1180
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 9 cut(s) 77, 340, 446, 512, 757, 764, 908, 916, 1251
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 292, 569, 1054
HpyF3I CTNAG 5 cut(s) 117, 291, 519, 891, 1092
HpySE526I ACGT 1 cut(s) 57
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 2 cut(s) 129, 1096
KpnI GGTACC 1 cut(s) 479
KroI GCCGGC 1 cut(s) 112
KroNI GCCGGC 1 cut(s) 114
Kzo9I GATC 3 cut(s) 596, 1112, 1172
LguI GCTCTTC 1 cut(s) 353
LmnI GCTCC 4 cut(s) 252, 400, 676, 696
Lsp1109I GCAGC 3 cut(s) 89, 324, 463
LweI GCATC 3 cut(s) 64, 433, 773
MaeI CTAG 4 cut(s) 99, 413, 825, 957
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 3 cut(s) 436, 613, 800
MalI GATC 3 cut(s) 598, 1114, 1174
MboI GATC 3 cut(s) 596, 1112, 1172
MboII GAAGA 4 cut(s) 366, 370, 879, 1050
MflI RGATCY 1 cut(s) 596
MhlI GDGCHC 6 cut(s) 138, 257, 426, 574, 701, 1253
MluCI AATT 9 cut(s) 460, 632, 660, 716, 725, 1016, 1021, 1049, 1271
MlyI GAGTC 2 cut(s) 155, 1151
MmeI TCCRAC 2 cut(s) 713, 1210
Mph1103I ATGCAT 1 cut(s) 766
MroNI GCCGGC 1 cut(s) 112
MseI TTAA 5 cut(s) 210, 924, 939, 1020, 1085
MslI CAYNNNNRTG 1 cut(s) 795
MspI CCGG 3 cut(s) 113, 198, 713
MspR9I CCNGG 1 cut(s) 250
Mva1269I GAATGC 1 cut(s) 918
MvaI CCWGG 1 cut(s) 250
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 4 cut(s) 128, 292, 569, 1054
NaeI GCCGGC 1 cut(s) 114
NdeII GATC 3 cut(s) 596, 1112, 1172
NgoMIV GCCGGC 1 cut(s) 112
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 3 cut(s) 322, 477, 672
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 2 cut(s) 613, 800
NsiI ATGCAT 1 cut(s) 766
NspI RCATGY 1 cut(s) 344
PceI AGGCCT 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 918
PdiI GCCGGC 1 cut(s) 114
PfeI GAWTC 2 cut(s) 327, 860
PflMI CCANNNNNTGG 1 cut(s) 1109
PinAI ACCGGT 1 cut(s) 197
PkrI GCNGC 4 cut(s) 79, 128, 339, 453
PleI GAGTC 2 cut(s) 154, 1151
PpsI GAGTC 2 cut(s) 154, 1151
Psp124BI GAGCTC 2 cut(s) 138, 257
Psp6I CCWGG 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 1131
PspGI CCWGG 1 cut(s) 248
PspN4I GGNNCC 3 cut(s) 322, 477, 672
PsuI RGATCY 1 cut(s) 596
RsaI GTAC 4 cut(s) 86, 477, 1008, 1044
RsaNI GTAC 4 cut(s) 85, 476, 1007, 1043
RseI CAYNNNNRTG 1 cut(s) 795
SacI GAGCTC 2 cut(s) 138, 257
SapI GCTCTTC 1 cut(s) 353
SaqAI TTAA 5 cut(s) 210, 924, 939, 1020, 1085
SatI GCNGC 4 cut(s) 78, 127, 338, 452
Sau3AI GATC 3 cut(s) 596, 1112, 1172
SchI GAGTC 2 cut(s) 155, 1151
ScrFI CCNGG 1 cut(s) 250
SduI GDGCHC 6 cut(s) 138, 257, 426, 574, 701, 1253
SfaNI GCATC 3 cut(s) 64, 433, 773
SmiMI CAYNNNNRTG 1 cut(s) 795
SmlI CTYRAG 2 cut(s) 734, 1239
SmoI CTYRAG 2 cut(s) 734, 1239
Sse9I AATT 9 cut(s) 460, 632, 660, 716, 725, 1016, 1021, 1049, 1271
SseBI AGGCCT 1 cut(s) 586
SsiI CCGC 4 cut(s) 127, 150, 842, 1055
SspI AATATT 1 cut(s) 970
SspMI CTAG 4 cut(s) 99, 413, 825, 957
SstI GAGCTC 2 cut(s) 138, 257
StuI AGGCCT 1 cut(s) 586
StyD4I CCNGG 1 cut(s) 248
TaaI ACNGT 4 cut(s) 838, 880, 1006, 1180
TaiI ACGT 1 cut(s) 60
TaqI TCGA 6 cut(s) 72, 144, 172, 628, 666, 1175
TasI AATT 9 cut(s) 460, 632, 660, 716, 725, 1016, 1021, 1049, 1271
TatI WGTACW 1 cut(s) 1042
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 327, 860
Tru1I TTAA 5 cut(s) 210, 924, 939, 1020, 1085
Tru9I TTAA 5 cut(s) 210, 924, 939, 1020, 1085
TscAI CASTG 2 cut(s) 885, 1009
TseFI GTSAC 2 cut(s) 613, 800
TseI GCWGC 3 cut(s) 77, 337, 451
Tsp45I GTSAC 2 cut(s) 613, 800
TspDTI ATGAA 4 cut(s) 281, 504, 666, 765
TspRI CASTG 2 cut(s) 885, 1009
Van91I CCANNNNNTGG 1 cut(s) 1109
VneI GTGCAC 1 cut(s) 1249
XapI RAATTY 5 cut(s) 632, 660, 716, 725, 1016
XceI RCATGY 1 cut(s) 344
XcmI CCANNNNNNNNNTGG 2 cut(s) 1009, 1106
XspI CTAG 4 cut(s) 99, 413, 825, 957
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.