pycom04g07660

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0006281
Physical Location & Seq
Forward (+)
8429861 .. 8432787
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1905 bp
ATGCTCAGCCAGGAGATTGTGTTTAAAAAGCAGGTTCATCAACTCCATCATCTATATAGGATACAGAAGACGCTGATGCAGAACCTTGGCCCAGTGGAGTTTGGTAGATACAATTATAACATAGCTGGAGCTGAGTCGAATCTTTTACCTCTGAAAAATTATGCTAGAGATGAACCTATGGACTTGTTAGAAGGGTGCAAGGGTGTAGATGATAAGATCCAGCCGAGGACTCCTGAGCTTAAACTTCCCTCTGACCAATTTCTTAGCTATATTGATCCAGCTGAGGTGAAGCTGTCCCTGAGTATTCAAGAGGACAATAGGATAGAGGGAGGTACTATGAGGACTTGGTTTGATTTAGAAACTCATTCCGGCTCGCAACACGTGATTGATTTAGAAGAATCGATTGAAAGGACATCAAATGAGGGTTTGGAGCAGTCACCTTCATTCAGCAGTACTGCTCCAATTACAAGCGTCAGGGGAACCCACAATTTAGAAGTTTCTGTCCTTTTTGATCCGATCATCTCAAGTAGTGTGAAGAAAAATCTATTGTTTCATGCTGCCGAGAGTTACCCTCATTTAGATGGCAGGGAGAGGAATCCTTTCAATCAAGGATTAGAAAAGTGTTCCGGTGGCATTGCCATTAACAATTCTTCCACCAGGAGGGAGCTGTTAAGTTCATATGAAACAGGGGTTCTGGACCTCAACAAAATTCAGTATGATGATTCATCTTGTCTCTCAAATGATCGTACAGTAGCCCATCTTTCAACCGCTAGCTCGTCACATTTACACAGATTAGTTGGCAAGGTAGAGGAGGGCACTCATTGTTTCGGAACTGGGAAGAAGCAAAATAGTAATAGCTCAATTGAAAGTTCTGAAAGGTTTCAAAAATACAATGCTGGAAATTTGAAAGTTGATGGATCGAGCATAAGCGGAATGGGCAATGAAAGCTTGGAGGCTGCGTCTAGATCAAAGATAGACCCCTGTGAAGCAGTTGGTTTCCACAGTGGTGACCCCAGTAATGAAAACAATGGGTTCATCATGAAACTCCTAAACAGTCGAAGTAGTTCATGTACTTCTGCTAAACAAGTGAATTGTGAGAACAACAAAATAGAAGACCCCGTATTTCCATATACTGATAATTGTCAAAAAACTGATCGAGACGGACATGGCAACATATCTTCTGCTTCATGTAAACCATGCTGCAATGCTGAAGATGATTCCAGCAGCGGAAAGACTCTGCAGTCTGAGATTGAATTCGGAAACTCAAATCCCCTTTCCGTTGATCGATCTTCAGGAACACATGGAGGGTCTCAAGTTTCAGAAACCTTAATGGGTGAGCAGGACCGAAGATCTTCTGACAACAATGAATTAAAACACAAAGACCTCAACAAGGGAGGAGATTCAGCTGAACTGGATGTTTTGATCAGAGCAGCAGCTGAAGCACTTGTCGGAATCTCATTGGAGAGTTCATCCTGTTATCAAGATTGCGCTTATGAAGTAGGAGGTTCAACAAAGATGGAAACGAAGGAGAGTGAACAACCGCAGTGTTCTGATTCTTATGAGTTACTCACACTAAATCTAGCAGGGAGCACTGAAGATACCTATTGTGTGTCATCAAAGCCTTCTGAAGTAAATTACAATGACAGTAAGGAATTTGGCGTTAAGTTGAGAAGGGGGAGGAGAATGAAAGATTTTCAGAGGGACGTTCTTCCTGGTCTGGCTTCTCTTTCCAGACATGAAATCCGTGAAGATATTACCATTATGGAGACAGTTTTAAGATCAAGGGAGTACAAAAAATTAAGAGGCAAGGTGGTTGATGGTCAAAGCTGCTGTAGTCCTGTCAAGCACAAACGTTCGCGACTCAACTATGTTCCGCGGAGGAAAAGAAATTTACGAGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

635

Amino Acids

70.22

Weight (kDa)

5.78

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 491 - 593 2.3e-16 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 117
AasI GACNNNNNNGTC 1 cut(s) 1240
Acc36I ACCTGC 1 cut(s) 22
AccII CGCG 2 cut(s) 1858, 1876
AciI CCGC 6 cut(s) 768, 930, 1227, 1541, 1874, 1876
AclI AACGTT 1 cut(s) 1852
AclWI GGATC 4 cut(s) 211, 269, 506, 925
AcsI RAATTY 5 cut(s) 708, 901, 1253, 1652, 1888
AcuI CTGAAG 5 cut(s) 1230, 1275, 1458, 1614, 1647
AcvI CACGTG 1 cut(s) 382
AfaI GTAC 5 cut(s) 334, 454, 748, 1072, 1790
AfiI CCNNNNNNNGG 2 cut(s) 660, 1390
AflIII ACRYGT 1 cut(s) 379
AgsI TTSAA 9 cut(s) 308, 407, 604, 765, 866, 884, 907, 1253, 1509
AjnI CCWGG 3 cut(s) 9, 656, 1711
AleI CACNNNNGTG 1 cut(s) 1005
Alw21I GWGCWC 1 cut(s) 1592
Alw26I GTCTC 4 cut(s) 737, 1152, 1314, 1760
AlwI GGATC 4 cut(s) 211, 269, 506, 925
AlwNI CAGNNNCTG 1 cut(s) 1436
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 7 cut(s) 557, 956, 1200, 1224, 1430, 1433, 1827
ApoI RAATTY 5 cut(s) 708, 901, 1253, 1652, 1888
Asp700I GAANNNNTTC 4 cut(s) 599, 879, 1063, 1351
AspLEI GCGC 1 cut(s) 1490
AspS9I GGNCC 3 cut(s) 89, 697, 1342
AsuHPI GGTGA 4 cut(s) 298, 429, 1019, 1346
AsuNHI GCTAGC 1 cut(s) 770
AvaII GGWCC 2 cut(s) 697, 1342
BaeGI GKGCMC 1 cut(s) 818
BbrPI CACGTG 1 cut(s) 382
BbsI GAAGAC 2 cut(s) 74, 1119
Bbv12I GWGCWC 1 cut(s) 1592
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 7 cut(s) 544, 943, 1187, 1236, 1442, 1445, 1814
BccI CCATC 6 cut(s) 54, 575, 765, 908, 1510, 1811
BciT130I CCWGG 3 cut(s) 11, 658, 1713
BciVI GTATCC 1 cut(s) 54
BclI TGATCA 1 cut(s) 1422
BcoDI GTCTC 4 cut(s) 737, 1152, 1314, 1760
BfaI CTAG 4 cut(s) 165, 771, 963, 1580
BfmI CTRYAG 2 cut(s) 1238, 1831
BfuAI ACCTGC 1 cut(s) 22
BfuI GTATCC 1 cut(s) 54
BglII AGATCT 1 cut(s) 1349
BisI GCNGC 7 cut(s) 558, 957, 1201, 1225, 1431, 1434, 1828
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 7 cut(s) 559, 958, 1202, 1226, 1432, 1435, 1829
BmcAI AGTACT 1 cut(s) 454
Bme1390I CCNGG 3 cut(s) 11, 658, 1713
Bme18I GGWCC 2 cut(s) 697, 1342
BmgT120I GGNCC 3 cut(s) 89, 697, 1342
BmiI GGNNCC 1 cut(s) 481
BmrFI CCNGG 3 cut(s) 11, 658, 1713
BmrI ACTGGG 3 cut(s) 86, 843, 1008
BmsI GCATC 1 cut(s) 66
BmtI GCTAGC 1 cut(s) 774
BmuI ACTGGG 3 cut(s) 86, 843, 1008
BpiI GAAGAC 2 cut(s) 74, 1119
BplI GAGNNNNNCTC 2 cut(s) 556, 588
BpmI CTGGAG 1 cut(s) 147
Bpu10I CCTNAGC 2 cut(s) 234, 282
Bpu1102I GCTNAGC 1 cut(s) 5
BpuEI CTTGAG 2 cut(s) 508, 1296
Bsa29I ATCGAT 2 cut(s) 401, 1285
BsaAI YACGTR 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 1314
BsaJI CCNNGG 3 cut(s) 85, 224, 1874
BsaWI WCCGGW 1 cut(s) 626
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 944, 974
Bsc4I CCNNNNNNNGG 2 cut(s) 660, 1390
Bse1I ACTGG 4 cut(s) 92, 838, 1014, 1416
Bse3DI GCAATG 3 cut(s) 633, 946, 1210
BseBI CCWGG 3 cut(s) 11, 658, 1713
BseCI ATCGAT 2 cut(s) 401, 1285
BseDI CCNNGG 3 cut(s) 85, 224, 1874
BseGI GGATG 2 cut(s) 1420, 1469
BseLI CCNNNNNNNGG 2 cut(s) 660, 1390
BseMI GCAATG 3 cut(s) 633, 946, 1210
BseMII CTCAG 6 cut(s) 19, 123, 225, 273, 290, 1236
BseNI ACTGG 4 cut(s) 92, 838, 1014, 1416
BseRI GAGGAG 3 cut(s) 824, 1410, 1693
BseSI GKGCMC 1 cut(s) 818
BseXI GCAGC 7 cut(s) 544, 943, 1187, 1236, 1442, 1445, 1814
Bsh1236I CGCG 2 cut(s) 1858, 1876
BshFI GGCC 1 cut(s) 90
BshVI ATCGAT 2 cut(s) 401, 1285
BsiHKAI GWGCWC 1 cut(s) 1592
BsiSI CCGG 2 cut(s) 369, 627
BslFI GGGAC 2 cut(s) 280, 1715
BslI CCNNNNNNNGG 2 cut(s) 660, 1390
BsmAI GTCTC 4 cut(s) 737, 1152, 1314, 1760
BsmBI CGTCTC 1 cut(s) 1152
BsmFI GGGAC 2 cut(s) 280, 1715
BsnI GGCC 1 cut(s) 90
Bso31I GGTCTC 1 cut(s) 1314
Bsp1286I GDGCHC 2 cut(s) 818, 1592
Bsp1720I GCTNAGC 1 cut(s) 5
Bsp68I TCGCGA 1 cut(s) 1858
BspACI CCGC 6 cut(s) 768, 930, 1227, 1541, 1874, 1876
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 6 cut(s) 18, 124, 226, 274, 291, 1237
BspDI ATCGAT 2 cut(s) 401, 1285
BspFNI CGCG 2 cut(s) 1858, 1876
BspHI TCATGA 1 cut(s) 1038
BspLI GGNNCC 1 cut(s) 481
BspMAI CTGCAG 1 cut(s) 1242
BspMI ACCTGC 1 cut(s) 22
BspOI GCTAGC 1 cut(s) 774
BspPI GGATC 4 cut(s) 211, 269, 506, 925
BspTNI GGTCTC 1 cut(s) 1314
BsrDI GCAATG 3 cut(s) 633, 946, 1210
BsrI ACTGG 4 cut(s) 92, 838, 1014, 1416
BssECI CCNNGG 3 cut(s) 85, 224, 1874
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 3 cut(s) 11, 658, 1713
Bst4CI ACNGT 5 cut(s) 751, 1004, 1055, 1646, 1771
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 374, 772
BstDEI CTNAG 7 cut(s) 5, 132, 234, 263, 282, 299, 1245
BstDSI CCRYGG 1 cut(s) 1874
BstEII GGTNACC 1 cut(s) 1007
BstENI CCTNNNNNAGG 1 cut(s) 1388
BstF5I GGATG 2 cut(s) 1420, 1469
BstFNI CGCG 2 cut(s) 1858, 1876
BstHHI GCGC 1 cut(s) 1490
BstMAI GTCTC 4 cut(s) 737, 1152, 1314, 1760
BstMWI GCNNNNNNNGC 3 cut(s) 936, 945, 1439
BstNI CCWGG 3 cut(s) 11, 658, 1713
BstPI GGTNACC 1 cut(s) 1007
BstSCI CCNGG 3 cut(s) 9, 656, 1711
BstSFI CTRYAG 2 cut(s) 1238, 1831
BstSLI GKGCMC 1 cut(s) 818
BstUI CGCG 2 cut(s) 1858, 1876
BstV1I GCAGC 7 cut(s) 544, 943, 1187, 1236, 1442, 1445, 1814
BstV2I GAAGAC 2 cut(s) 74, 1119
BstX2I RGATCY 2 cut(s) 216, 1349
BstYI RGATCY 2 cut(s) 216, 1349
Bsu15I ATCGAT 2 cut(s) 401, 1285
BsuI GTATCC 1 cut(s) 54
BsuRI GGCC 1 cut(s) 90
BsuTUI ATCGAT 2 cut(s) 401, 1285
BtgI CCRYGG 1 cut(s) 1874
BtsCI GGATG 2 cut(s) 1420, 1469
BtsI GCAGTG 1 cut(s) 1550
BtsIMutI CAGTG 4 cut(s) 99, 1009, 1550, 1590
BtuMI TCGCGA 1 cut(s) 1858
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 2 cut(s) 374, 772
CaiI CAGNNNCTG 1 cut(s) 1436
CciI TCATGA 1 cut(s) 1038
CfoI GCGC 1 cut(s) 1490
Cfr13I GGNCC 3 cut(s) 89, 697, 1342
Cfr42I CCGCGG 1 cut(s) 1877
ClaI ATCGAT 2 cut(s) 401, 1285
CseI GACGC 3 cut(s) 79, 460, 948
Csp6I GTAC 5 cut(s) 333, 453, 747, 1071, 1789
CviAII CATG 8 cut(s) 554, 1039, 1068, 1166, 1188, 1197, 1301, 1736
CviQI GTAC 5 cut(s) 333, 453, 747, 1071, 1789
DdeI CTNAG 7 cut(s) 5, 132, 234, 263, 282, 299, 1245
DraI TTTAAA 1 cut(s) 25
DrdI GACNNNNNNGTC 1 cut(s) 1240
DseDI GACNNNNNNGTC 1 cut(s) 1240
Eco130I CCWWGG 1 cut(s) 85
Eco31I GGTCTC 1 cut(s) 1314
Eco47I GGWCC 2 cut(s) 697, 1342
Eco57I CTGAAG 5 cut(s) 1230, 1275, 1458, 1614, 1647
Eco72I CACGTG 1 cut(s) 382
Eco91I GGTNACC 1 cut(s) 1007
EcoNI CCTNNNNNAGG 1 cut(s) 1388
EcoO65I GGTNACC 1 cut(s) 1007
EcoRI GAATTC 1 cut(s) 1253
EcoRII CCWGG 3 cut(s) 9, 656, 1711
EcoT14I CCWWGG 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 85
Esp3I CGTCTC 1 cut(s) 1152
FaeI CATG 8 cut(s) 557, 1042, 1071, 1169, 1191, 1200, 1304, 1739
FaqI GGGAC 2 cut(s) 280, 1715
FatI CATG 8 cut(s) 553, 1038, 1067, 1165, 1187, 1196, 1300, 1735
FauNDI CATATG 1 cut(s) 679
FbaI TGATCA 1 cut(s) 1422
Fnu4HI GCNGC 7 cut(s) 558, 957, 1201, 1225, 1431, 1434, 1828
FokI GGATG 2 cut(s) 1427, 1456
Fsp4HI GCNGC 7 cut(s) 558, 957, 1201, 1225, 1431, 1434, 1828
FspBI CTAG 4 cut(s) 165, 771, 963, 1580
GlaI GCGC 1 cut(s) 1489
GluI GCNGC 7 cut(s) 558, 957, 1201, 1225, 1431, 1434, 1828
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 1 cut(s) 90
HapII CCGG 2 cut(s) 369, 627
HgaI GACGC 3 cut(s) 79, 460, 948
HhaI GCGC 1 cut(s) 1490
Hin1II CATG 8 cut(s) 557, 1042, 1071, 1169, 1191, 1200, 1304, 1739
Hin6I GCGC 1 cut(s) 1488
HinP1I GCGC 1 cut(s) 1488
HindIII AAGCTT 2 cut(s) 946, 1899
HpaII CCGG 2 cut(s) 369, 627
HphI GGTGA 4 cut(s) 298, 429, 1019, 1346
Hpy166II GTNNAC 2 cut(s) 1193, 1535
Hpy8I GTNNAC 2 cut(s) 1193, 1535
HpyAV CCTTC 5 cut(s) 185, 450, 1519, 1632, 1665
HpyCH4III ACNGT 5 cut(s) 751, 1004, 1055, 1646, 1771
HpyCH4IV ACGT 3 cut(s) 381, 1704, 1852
HpyCH4V TGCA 4 cut(s) 79, 198, 1203, 1240
HpyF10VI GCNNNNNNNGC 3 cut(s) 936, 945, 1439
HpyF3I CTNAG 7 cut(s) 5, 132, 234, 263, 282, 299, 1245
HpySE526I ACGT 3 cut(s) 381, 1704, 1852
Hsp92II CATG 8 cut(s) 557, 1042, 1071, 1169, 1191, 1200, 1304, 1739
HspAI GCGC 1 cut(s) 1488
Ksp22I TGATCA 1 cut(s) 1422
KspI CCGCGG 1 cut(s) 1877
LmnI GCTCC 5 cut(s) 128, 430, 463, 664, 1587
Lsp1109I GCAGC 7 cut(s) 544, 943, 1187, 1236, 1442, 1445, 1814
LweI GCATC 1 cut(s) 66
MaeI CTAG 4 cut(s) 165, 771, 963, 1580
MaeII ACGT 3 cut(s) 381, 1704, 1852
MaeIII GTNAC 5 cut(s) 435, 566, 777, 1007, 1563
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 2 cut(s) 216, 1349
MhlI GDGCHC 2 cut(s) 818, 1592
MlyI GAGTC 4 cut(s) 143, 223, 1228, 1854
MmeI TCCRAC 1 cut(s) 1429
MroXI GAANNNNTTC 4 cut(s) 599, 879, 1063, 1351
MseI TTAA 9 cut(s) 24, 240, 642, 671, 1328, 1370, 1662, 1775, 1799
MslI CAYNNNNRTG 2 cut(s) 579, 1005
MspA1I CMGCKG 5 cut(s) 281, 1227, 1406, 1436, 1876
MspI CCGG 2 cut(s) 369, 627
MspR9I CCNGG 3 cut(s) 11, 658, 1713
MunI CAATTG 1 cut(s) 861
MvaI CCWGG 3 cut(s) 11, 658, 1713
MvnI CGCG 2 cut(s) 1858, 1876
MwoI GCNNNNNNNGC 3 cut(s) 936, 945, 1439
NdeI CATATG 1 cut(s) 679
NheI GCTAGC 1 cut(s) 770
NlaIII CATG 8 cut(s) 557, 1042, 1071, 1169, 1191, 1200, 1304, 1739
NlaIV GGNNCC 1 cut(s) 481
NmeAIII GCCGAG 2 cut(s) 249, 586
NmuCI GTSAC 3 cut(s) 435, 777, 1007
NruI TCGCGA 1 cut(s) 1858
OliI CACNNNNGTG 1 cut(s) 1005
PagI TCATGA 1 cut(s) 1038
PdmI GAANNNNTTC 4 cut(s) 599, 879, 1063, 1351
PfeI GAWTC 8 cut(s) 139, 398, 595, 722, 1217, 1400, 1452, 1553
PkrI GCNGC 7 cut(s) 559, 958, 1202, 1226, 1432, 1435, 1829
PleI GAGTC 4 cut(s) 142, 223, 1228, 1854
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
PpsI GAGTC 4 cut(s) 142, 223, 1228, 1854
Ppu21I YACGTR 1 cut(s) 382
PsiI TTATAA 1 cut(s) 117
Psp1406I AACGTT 1 cut(s) 1852
Psp6I CCWGG 3 cut(s) 9, 656, 1711
PspCI CACGTG 1 cut(s) 382
PspEI GGTNACC 1 cut(s) 1007
PspGI CCWGG 3 cut(s) 9, 656, 1711
PspN4I GGNNCC 1 cut(s) 481
PspPI GGNCC 3 cut(s) 89, 697, 1342
PstI CTGCAG 1 cut(s) 1242
PstNI CAGNNNCTG 1 cut(s) 1436
PsuI RGATCY 2 cut(s) 216, 1349
PvuII CAGCTG 3 cut(s) 281, 1406, 1436
RruI TCGCGA 1 cut(s) 1858
RsaI GTAC 5 cut(s) 334, 454, 748, 1072, 1790
RsaNI GTAC 5 cut(s) 333, 453, 747, 1071, 1789
RseI CAYNNNNRTG 2 cut(s) 579, 1005
SacII CCGCGG 1 cut(s) 1877
SaqAI TTAA 9 cut(s) 24, 240, 642, 671, 1328, 1370, 1662, 1775, 1799
SatI GCNGC 7 cut(s) 558, 957, 1201, 1225, 1431, 1434, 1828
Sau96I GGNCC 3 cut(s) 89, 697, 1342
ScaI AGTACT 1 cut(s) 454
SchI GAGTC 4 cut(s) 143, 223, 1228, 1854
ScrFI CCNGG 3 cut(s) 11, 658, 1713
SduI GDGCHC 2 cut(s) 818, 1592
SfaNI GCATC 1 cut(s) 66
SfcI CTRYAG 2 cut(s) 1238, 1831
Sfr303I CCGCGG 1 cut(s) 1877
SgrBI CCGCGG 1 cut(s) 1877
SinI GGWCC 2 cut(s) 697, 1342
SmiMI CAYNNNNRTG 2 cut(s) 579, 1005
SmlI CTYRAG 2 cut(s) 523, 1311
SmoI CTYRAG 2 cut(s) 523, 1311
SsiI CCGC 6 cut(s) 768, 930, 1227, 1541, 1874, 1876
SspMI CTAG 4 cut(s) 165, 771, 963, 1580
StyD4I CCNGG 3 cut(s) 9, 656, 1711
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 5 cut(s) 751, 1004, 1055, 1646, 1771
TaiI ACGT 3 cut(s) 384, 1707, 1855
TaqI TCGA 6 cut(s) 137, 401, 920, 1057, 1156, 1285
TaqII GACCGA 1 cut(s) 1359
TatI WGTACW 3 cut(s) 452, 1070, 1788
TfiI GAWTC 8 cut(s) 139, 398, 595, 722, 1217, 1400, 1452, 1553
Tru1I TTAA 9 cut(s) 24, 240, 642, 671, 1328, 1370, 1662, 1775, 1799
Tru9I TTAA 9 cut(s) 24, 240, 642, 671, 1328, 1370, 1662, 1775, 1799
TscAI CASTG 4 cut(s) 99, 1009, 1550, 1597
TseFI GTSAC 3 cut(s) 435, 777, 1007
TseI GCWGC 7 cut(s) 557, 956, 1200, 1224, 1430, 1433, 1827
Tsp45I GTSAC 3 cut(s) 435, 777, 1007
TspGWI ACGGA 3 cut(s) 1176, 1267, 1733
TspRI CASTG 4 cut(s) 99, 1009, 1550, 1597
VpaK11BI GGWCC 2 cut(s) 697, 1342
XagI CCTNNNNNAGG 1 cut(s) 1388
XapI RAATTY 5 cut(s) 708, 901, 1253, 1652, 1888
XbaI TCTAGA 1 cut(s) 962
XmnI GAANNNNTTC 4 cut(s) 599, 879, 1063, 1351
XspI CTAG 4 cut(s) 165, 771, 963, 1580
ZrmI AGTACT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.