pycom04g10970

Soluble inorganic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007013
Physical Location & Seq
Forward (+)
13787020 .. 13789229
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGACCAGGAGGTCTATCGCTGCCCACCCATGGCATGACCTTGAAATCGGACCTGGAGCTCCTAAGATTTTCAACTGTGTTATCGAAATTCCAAAAGGAAGCAAAGTGAAATATGAGCTTGACAAAAAAACTGGACTCATCAAGGTTGACCGCATACTGTACTCATCTGTTGTCTATCCCCATAACTATGGTTTCATCCCCCGTACTCTTTGTGAGGACAATGACCCCTTGGATGTCTTGATTATTATGCAGGAGCCAGTTGTTCCTGGATGCTTTCTTCGTGCCAAAGCTATTGGCCTGATGCCTATGATTGACCAGGGTGAGAAAGATGACAAGATCATTGCTGTTTGTGCTGATGATCCTGAGTACCGACACTACAATGACATCAAGGAGCTCCCACCTCACCGTTTGGCTGAAATCCGGCGCTTCTTTGAGGACTACAAGAAAAATGAGAACAAAGAAGTTGCAGTTGACGACTTTCTCCCTGCATCTACTGCCTTTGACGCAATCCAGCATTCCATGAACCTTTATGCAGACTACATTGTGGAGAGCCTCAGGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Pyrophosphatase

Protein Analysis

188

Amino Acids

21.49

Weight (kDa)

5.69

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 26 - 177 1.6e-60 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AciI CCGC 2 cut(s) 151, 559
AclWI GGATC 1 cut(s) 353
AcsI RAATTY 1 cut(s) 87
AfaI GTAC 3 cut(s) 161, 205, 368
AfiI CCNNNNNNNGG 1 cut(s) 30
AgsI TTSAA 2 cut(s) 44, 73
AjnI CCWGG 4 cut(s) 5, 52, 265, 315
AluBI AGCT 4 cut(s) 59, 118, 290, 394
AluI AGCT 4 cut(s) 59, 118, 290, 394
Alw21I GWGCWC 2 cut(s) 61, 396
AlwI GGATC 1 cut(s) 353
AoxI GGCC 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 1 cut(s) 87
AspLEI GCGC 1 cut(s) 426
AspS9I GGNCC 1 cut(s) 50
AsuHPI GGTGA 2 cut(s) 332, 395
AvaII GGWCC 1 cut(s) 50
AxyI CCTNAGG 1 cut(s) 554
BanII GRGCYC 2 cut(s) 61, 396
Bbv12I GWGCWC 2 cut(s) 61, 396
BbvI GCAGC 1 cut(s) 7
BciT130I CCWGG 4 cut(s) 7, 54, 267, 317
BfoI RGCGCY 1 cut(s) 427
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 4 cut(s) 7, 54, 267, 317
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 4 cut(s) 7, 54, 267, 317
BmsI GCATC 3 cut(s) 260, 291, 497
BpmI CTGGAG 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 29, 228, 316
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 136, 257
Bse21I CCTNAGG 1 cut(s) 554
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 4 cut(s) 7, 54, 267, 317
BseDI CCNNGG 3 cut(s) 29, 228, 316
BseGI GGATG 3 cut(s) 195, 238, 275
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 2 cut(s) 136, 257
BseXI GCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 297
BsiHKAI GWGCWC 2 cut(s) 61, 396
BsiSI CCGG 1 cut(s) 421
BslI CCNNNNNNNGG 1 cut(s) 30
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 1 cut(s) 297
Bsp1286I GDGCHC 2 cut(s) 61, 396
Bsp143I GATC 2 cut(s) 336, 358
Bsp19I CCATGG 1 cut(s) 29
BspACI CCGC 2 cut(s) 151, 559
BspANI GGCC 1 cut(s) 297
BspCNI CTCAG 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 339
BsrI ACTGG 2 cut(s) 136, 257
BssECI CCNNGG 3 cut(s) 29, 228, 316
BssMI GATC 2 cut(s) 336, 358
BssT1I CCWWGG 2 cut(s) 29, 228
Bst2UI CCWGG 4 cut(s) 7, 54, 267, 317
Bst4CI ACNGT 3 cut(s) 77, 159, 407
BstAPI GCANNNNNTGC 1 cut(s) 494
BstDEI CTNAG 3 cut(s) 63, 363, 554
BstDSI CCRYGG 1 cut(s) 29
BstF5I GGATG 3 cut(s) 195, 238, 275
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 2 cut(s) 339, 361
BstMBI GATC 2 cut(s) 336, 358
BstMWI GCNNNNNNNGC 3 cut(s) 350, 494, 503
BstNI CCWGG 4 cut(s) 7, 54, 267, 317
BstSCI CCNGG 4 cut(s) 5, 52, 265, 315
BstV1I GCAGC 1 cut(s) 7
BstXI CCANNNNNNTGG 1 cut(s) 188
Bsu36I CCTNAGG 1 cut(s) 554
BsuRI GGCC 1 cut(s) 297
BtgI CCRYGG 1 cut(s) 29
BtsCI GGATG 3 cut(s) 195, 238, 275
CfoI GCGC 1 cut(s) 426
Cfr13I GGNCC 1 cut(s) 50
CseI GACGC 1 cut(s) 512
Csp6I GTAC 3 cut(s) 160, 204, 367
CviAII CATG 3 cut(s) 30, 35, 520
CviJI RGCY 8 cut(s) 59, 118, 256, 290, 297, 394, 413, 552
CviKI_1 RGCY 8 cut(s) 59, 118, 256, 290, 297, 394, 413, 552
CviQI GTAC 3 cut(s) 160, 204, 367
DdeI CTNAG 3 cut(s) 63, 363, 554
DpnI GATC 2 cut(s) 338, 360
DpnII GATC 2 cut(s) 336, 358
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Ecl136II GAGCTC 2 cut(s) 59, 394
Eco130I CCWWGG 2 cut(s) 29, 228
Eco24I GRGCYC 2 cut(s) 61, 396
Eco47I GGWCC 1 cut(s) 50
Eco53kI GAGCTC 2 cut(s) 59, 394
Eco81I CCTNAGG 1 cut(s) 554
EcoICRI GAGCTC 2 cut(s) 59, 394
EcoRII CCWGG 4 cut(s) 5, 52, 265, 315
EcoT14I CCWWGG 2 cut(s) 29, 228
EcoT38I GRGCYC 2 cut(s) 61, 396
ErhI CCWWGG 2 cut(s) 29, 228
FaeI CATG 3 cut(s) 33, 38, 523
FatI CATG 3 cut(s) 29, 34, 519
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 3 cut(s) 182, 245, 282
FriOI GRGCYC 2 cut(s) 61, 396
Fsp4HI GCNGC 1 cut(s) 21
GlaI GCGC 1 cut(s) 425
GluI GCNGC 1 cut(s) 21
GsuI CTGGAG 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 1 cut(s) 297
HapII CCGG 1 cut(s) 421
HgaI GACGC 1 cut(s) 512
HhaI GCGC 1 cut(s) 426
Hin1II CATG 3 cut(s) 33, 38, 523
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HincII GTYRAC 2 cut(s) 148, 472
HindII GTYRAC 2 cut(s) 148, 472
HinfI GANTC 1 cut(s) 135
HpaII CCGG 1 cut(s) 421
HphI GGTGA 2 cut(s) 332, 395
Hpy166II GTNNAC 2 cut(s) 148, 472
Hpy188I TCNGA 1 cut(s) 50
Hpy188III TCNNGA 2 cut(s) 238, 362
Hpy8I GTNNAC 2 cut(s) 148, 472
HpyCH4III ACNGT 3 cut(s) 77, 159, 407
HpyCH4V TGCA 4 cut(s) 250, 467, 488, 533
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 494, 503
HpyF3I CTNAG 3 cut(s) 63, 363, 554
Hsp92II CATG 3 cut(s) 33, 38, 523
HspAI GCGC 1 cut(s) 424
Kzo9I GATC 2 cut(s) 336, 358
LmnI GCTCC 5 cut(s) 56, 64, 253, 391, 399
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 3 cut(s) 260, 291, 497
MalI GATC 2 cut(s) 338, 360
MboI GATC 2 cut(s) 336, 358
MboII GAAGA 1 cut(s) 269
MhlI GDGCHC 2 cut(s) 61, 396
MluCI AATT 1 cut(s) 87
MlyI GAGTC 1 cut(s) 129
MnlI CCTC 5 cut(s) 3, 208, 411, 427, 563
MslI CAYNNNNRTG 2 cut(s) 186, 378
MspI CCGG 1 cut(s) 421
MspR9I CCNGG 4 cut(s) 7, 54, 267, 317
Mva1269I GAATGC 1 cut(s) 514
MvaI CCWGG 4 cut(s) 7, 54, 267, 317
MwoI GCNNNNNNNGC 3 cut(s) 350, 494, 503
NcoI CCATGG 1 cut(s) 29
NdeII GATC 2 cut(s) 336, 358
NlaIII CATG 3 cut(s) 33, 38, 523
NlaIV GGNNCC 1 cut(s) 255
PctI GAATGC 1 cut(s) 514
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
Psp124BI GAGCTC 2 cut(s) 61, 396
Psp6I CCWGG 4 cut(s) 5, 52, 265, 315
PspGI CCWGG 4 cut(s) 5, 52, 265, 315
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 1 cut(s) 50
RsaI GTAC 3 cut(s) 161, 205, 368
RsaNI GTAC 3 cut(s) 160, 204, 367
RseI CAYNNNNRTG 2 cut(s) 186, 378
SacI GAGCTC 2 cut(s) 61, 396
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 2 cut(s) 336, 358
Sau96I GGNCC 1 cut(s) 50
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 4 cut(s) 7, 54, 267, 317
SduI GDGCHC 2 cut(s) 61, 396
SfaNI GCATC 3 cut(s) 260, 291, 497
SinI GGWCC 1 cut(s) 50
SmiMI CAYNNNNRTG 2 cut(s) 186, 378
Sse9I AATT 1 cut(s) 87
SsiI CCGC 2 cut(s) 151, 559
SstI GAGCTC 2 cut(s) 61, 396
StyD4I CCNGG 4 cut(s) 5, 52, 265, 315
StyI CCWWGG 2 cut(s) 29, 228
TaaI ACNGT 3 cut(s) 77, 159, 407
TaqI TCGA 1 cut(s) 84
TasI AATT 1 cut(s) 87
TatI WGTACW 1 cut(s) 159
TseI GCWGC 1 cut(s) 20
TspDTI ATGAA 2 cut(s) 184, 536
VpaK11BI GGWCC 1 cut(s) 50
XapI RAATTY 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.