pycom04g14770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007096
Physical Location & Seq
Reverse (-)
17572715 .. 17574088
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g14770.1

Sequence Viewer

Length: 957 bp
ATGGAAGACCATTCCCTAGACAAGGTAGCGATGTCAGGCCCAGCCCTGGCCTCCACGATCCAACGCTTCTCCACCTCCCCAGGCTCCGTCGACGGCCTGATCTTCGGCCATGTCAGCCACATCCCCCCTACCCTCACCGACGACTCTCCCAATTCCACCTCCTCCGCCACTCTCATCGCCACCGTCACCGCCTTCTTCTGCTCCGGCTCCACCCTCTCCTTCTACAACTCCTCCGGCCGGGTCGACCCCCTCTCCCTCCGCCGCCTCGTCCAAGCCCAGCAGGCCCAATCCCCCTCCTCCCACCTCCTCGGCTGGTTCTCCGGCCGCCGTAGAACCCACCTCCGCCCCTCCCTCCGAGAATTCTCAGTCTCCAACTCCCTCTCCTCCAACCCCACTCTCAACTTCCCAATCCAAAACCCTCCCGAGAGCTCCGCCGCCGCCCCAACTCTCACCCCCTCCCTCTTTCTCCTCTTCGCTTCTCCGGTCACCGATCAGACCATCCACACTCACGAGTACCGTGCCTATCAGTTCCGGCCATCCAAGCACTCCTTCGAGCCGAAATCGATCCAGATCGTCAACATTGGGCCCGCGTTCCGAGCCCATTACGGGAATTTCAACCCTAGCTCGCCGTTCCCAATTTTGCCCTGCGAGCTTAGGGTTTCGCCGATGAATGTCGACAGGGGAGAGGAGAGCTTAGATCAACTCAAGCAGCACAACAAGGATCAGAGCGAACTTGATATGTGCAGTGAGGGTTTGGATGTTGGAAGTCTCAGTAGGCTGATGGGTTCGGAGGCCGCGAATTACACCGCCGGCGTCGAGGACTTGTATGAGAAAATGCTTGTCAAGATTGAAACTTTGACCAGGCTCGTTGAGCAGAGCTCCGCCAAGGTTCTTGAGCAGGAAAATCACAATAGGAAGCTAAGGTACAAAGCTGCTAGGTCTGCTGGCTTGGAGTAA

Protein Analysis

319

Amino Acids

34.93

Weight (kDa)

6.6

Isoelectric Point (pI)

60.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MPN_2A_DUB_like PF21125 11 - 195 1.5e-08 BRCA1-A complex subunit Abraxas 1 MPN domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 90, 243, 675
AccII CGCG 2 cut(s) 590, 797
AclWI GGATC 3 cut(s) 52, 559, 729
AcoI YGGCCR 4 cut(s) 106, 235, 322, 533
AcsI RAATTY 2 cut(s) 359, 610
AcyI GRCGYC 1 cut(s) 813
AfaI GTAC 2 cut(s) 515, 926
AfiI CCNNNNNNNGG 4 cut(s) 22, 46, 237, 606
AgsI TTSAA 2 cut(s) 616, 851
AjnI CCWGG 3 cut(s) 45, 79, 860
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
AluBI AGCT 7 cut(s) 429, 624, 652, 693, 879, 919, 932
AluI AGCT 7 cut(s) 429, 624, 652, 693, 879, 919, 932
Alw21I GWGCWC 2 cut(s) 431, 881
Alw26I GTCTC 2 cut(s) 373, 773
AlwI GGATC 3 cut(s) 52, 559, 729
Ama87I CYCGRG 1 cut(s) 422
ApaI GGGCCC 1 cut(s) 588
ApeKI GCWGC 2 cut(s) 709, 932
ApoI RAATTY 2 cut(s) 359, 610
AspS9I GGNCC 4 cut(s) 38, 283, 584, 585
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 4 cut(s) 127, 178, 442, 478
AvaI CYCGRG 1 cut(s) 422
BaeGI GKGCMC 1 cut(s) 588
BanII GRGCYC 4 cut(s) 431, 588, 601, 881
BarI GAAGNNNNNNTAC 2 cut(s) 908, 940
BauI CACGAG 1 cut(s) 509
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 431, 881
BbvI GCAGC 2 cut(s) 721, 919
BccI CCATC 3 cut(s) 506, 544, 775
BceAI ACGGC 3 cut(s) 109, 312, 613
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 3 cut(s) 47, 81, 862
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 2 cut(s) 373, 773
BfaI CTAG 3 cut(s) 17, 621, 936
BglI GCCNNNNNGGC 1 cut(s) 281
BisI GCNGC 7 cut(s) 262, 325, 435, 438, 710, 795, 933
BlsI GCNGC 7 cut(s) 263, 326, 436, 439, 711, 796, 934
Bme1390I CCNGG 4 cut(s) 47, 81, 239, 862
BmeT110I CYCGRG 1 cut(s) 422
BmgT120I GGNCC 4 cut(s) 38, 283, 584, 585
BmiI GGNNCC 3 cut(s) 85, 208, 586
BmrFI CCNGG 4 cut(s) 47, 81, 239, 862
BpiI GAAGAC 1 cut(s) 12
BplI GAGNNNNNCTC 2 cut(s) 863, 895
Bpu10I CCTNAGC 2 cut(s) 653, 920
BpuEI CTTGAG 2 cut(s) 689, 914
BpuMI CCSGG 1 cut(s) 239
Bsa29I ATCGAT 1 cut(s) 563
BsaBI GATNNNNATC 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 813
BsaJI CCNNGG 4 cut(s) 45, 79, 307, 885
BsaWI WCCGGW 1 cut(s) 481
BsaXI ACNNNNNCTCC 6 cut(s) 215, 236, 245, 266, 365, 395
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 46, 237, 606
Bse118I RCCGGY 1 cut(s) 809
Bse8I GATNNNNATC 1 cut(s) 569
BseBI CCWGG 3 cut(s) 47, 81, 862
BseCI ATCGAT 1 cut(s) 563
BseDI CCNNGG 4 cut(s) 45, 79, 307, 885
BseGI GGATG 4 cut(s) 120, 498, 536, 763
BseJI GATNNNNATC 1 cut(s) 569
BseLI CCNNNNNNNGG 4 cut(s) 22, 46, 237, 606
BseMII CTCAG 2 cut(s) 378, 784
BseRI GAGGAG 7 cut(s) 151, 220, 286, 296, 373, 458, 701
BseSI GKGCMC 1 cut(s) 588
BseX3I CGGCCG 2 cut(s) 235, 322
BseXI GCAGC 2 cut(s) 721, 919
BseYI CCCAGC 2 cut(s) 40, 276
BsgI GTGCAG 1 cut(s) 763
Bsh1236I CGCG 2 cut(s) 590, 797
Bsh1285I CGRYCG 2 cut(s) 238, 325
BshVI ATCGAT 1 cut(s) 563
BsiEI CGRYCG 2 cut(s) 238, 325
BsiHKAI GWGCWC 2 cut(s) 431, 881
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 7 cut(s) 204, 234, 238, 321, 482, 532, 810
BslI CCNNNNNNNGG 4 cut(s) 22, 46, 237, 606
BsmAI GTCTC 2 cut(s) 373, 773
BsoBI CYCGRG 1 cut(s) 422
Bsp120I GGGCCC 1 cut(s) 584
Bsp1286I GDGCHC 4 cut(s) 431, 588, 601, 881
Bsp143I GATC 7 cut(s) 57, 99, 490, 564, 570, 697, 721
BspCNI CTCAG 2 cut(s) 377, 783
BspDI ATCGAT 1 cut(s) 563
BspFNI CGCG 2 cut(s) 590, 797
BspLI GGNNCC 3 cut(s) 85, 208, 586
BspPI GGATC 3 cut(s) 52, 559, 729
BsrFI RCCGGY 1 cut(s) 809
BssAI RCCGGY 1 cut(s) 809
BssECI CCNNGG 4 cut(s) 45, 79, 307, 885
BssMI GATC 7 cut(s) 57, 99, 490, 564, 570, 697, 721
BssNI GRCGYC 1 cut(s) 813
BssSI CACGAG 1 cut(s) 509
BssT1I CCWWGG 1 cut(s) 885
Bst2BI CACGAG 1 cut(s) 509
Bst2UI CCWGG 3 cut(s) 47, 81, 862
Bst4CI ACNGT 2 cut(s) 184, 518
Bst6I CTCTTC 1 cut(s) 476
BstACI GRCGYC 1 cut(s) 813
BstC8I GCNNGC 6 cut(s) 282, 588, 626, 650, 811, 946
BstDEI CTNAG 5 cut(s) 364, 653, 694, 770, 920
BstEII GGTNACC 1 cut(s) 484
BstENI CCTNNNNNAGG 1 cut(s) 20
BstF5I GGATG 4 cut(s) 120, 498, 536, 763
BstFNI CGCG 2 cut(s) 590, 797
BstKTI GATC 7 cut(s) 60, 102, 493, 567, 573, 700, 724
BstMAI GTCTC 2 cut(s) 373, 773
BstMBI GATC 7 cut(s) 57, 99, 490, 564, 570, 697, 721
BstMCI CGRYCG 2 cut(s) 238, 325
BstMWI GCNNNNNNNGC 7 cut(s) 114, 281, 541, 596, 649, 871, 941
BstNI CCWGG 3 cut(s) 47, 81, 862
BstPI GGTNACC 1 cut(s) 484
BstSCI CCNGG 4 cut(s) 45, 79, 237, 860
BstSLI GKGCMC 1 cut(s) 588
BstUI CGCG 2 cut(s) 590, 797
BstV1I GCAGC 2 cut(s) 721, 919
BstV2I GAAGAC 1 cut(s) 12
BstZI CGGCCG 2 cut(s) 235, 322
Bsu15I ATCGAT 1 cut(s) 563
BsuTUI ATCGAT 1 cut(s) 563
BtgZI GCGATG 2 cut(s) 44, 160
BtsCI GGATG 4 cut(s) 120, 498, 536, 763
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 1 cut(s) 751
Cac8I GCNNGC 6 cut(s) 282, 588, 626, 650, 811, 946
Cfr10I RCCGGY 1 cut(s) 809
Cfr13I GGNCC 4 cut(s) 38, 283, 584, 585
ClaI ATCGAT 1 cut(s) 563
CseI GACGC 1 cut(s) 802
Csp6I GTAC 2 cut(s) 514, 925
CviAII CATG 1 cut(s) 110
CviQI GTAC 2 cut(s) 514, 925
DdeI CTNAG 5 cut(s) 364, 653, 694, 770, 920
DpnI GATC 7 cut(s) 59, 101, 492, 566, 572, 699, 723
DpnII GATC 7 cut(s) 57, 99, 490, 564, 570, 697, 721
EaeI YGGCCR 4 cut(s) 106, 235, 322, 533
EagI CGGCCG 2 cut(s) 235, 322
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
EciI GGCGGA 5 cut(s) 154, 248, 332, 421, 871
Ecl136II GAGCTC 2 cut(s) 429, 879
EclXI CGGCCG 2 cut(s) 235, 322
Eco130I CCWWGG 1 cut(s) 885
Eco24I GRGCYC 4 cut(s) 431, 588, 601, 881
Eco52I CGGCCG 2 cut(s) 235, 322
Eco53kI GAGCTC 2 cut(s) 429, 879
Eco88I CYCGRG 1 cut(s) 422
Eco91I GGTNACC 1 cut(s) 484
EcoICRI GAGCTC 2 cut(s) 429, 879
EcoNI CCTNNNNNAGG 1 cut(s) 20
EcoO65I GGTNACC 1 cut(s) 484
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 45, 79, 860
EcoT14I CCWWGG 1 cut(s) 885
EcoT38I GRGCYC 4 cut(s) 431, 588, 601, 881
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 1 cut(s) 113
FaiI YATR 3 cut(s) 111, 740, 828
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FatI CATG 1 cut(s) 109
FauI CCCGC 1 cut(s) 595
FblI GTMKAC 3 cut(s) 90, 243, 675
Fnu4HI GCNGC 7 cut(s) 262, 325, 435, 438, 710, 795, 933
FokI GGATG 4 cut(s) 107, 485, 523, 770
FriOI GRGCYC 4 cut(s) 431, 588, 601, 881
Fsp4HI GCNGC 7 cut(s) 262, 325, 435, 438, 710, 795, 933
FspBI CTAG 3 cut(s) 17, 621, 936
GluI GCNGC 7 cut(s) 262, 325, 435, 438, 710, 795, 933
GsaI CCCAGC 2 cut(s) 44, 280
HapII CCGG 7 cut(s) 204, 234, 238, 321, 482, 532, 810
HgaI GACGC 1 cut(s) 802
Hin1I GRCGYC 1 cut(s) 813
Hin1II CATG 1 cut(s) 113
HincII GTYRAC 4 cut(s) 91, 244, 577, 676
HindII GTYRAC 4 cut(s) 91, 244, 577, 676
HinfI GANTC 1 cut(s) 143
HpaII CCGG 7 cut(s) 204, 234, 238, 321, 482, 532, 810
HphI GGTGA 4 cut(s) 127, 178, 442, 478
Hpy166II GTNNAC 4 cut(s) 91, 244, 577, 676
Hpy188I TCNGA 5 cut(s) 356, 495, 596, 726, 790
Hpy188III TCNNGA 5 cut(s) 422, 509, 568, 844, 893
Hpy8I GTNNAC 4 cut(s) 91, 244, 577, 676
Hpy99I CGWCG 4 cut(s) 92, 95, 143, 818
HpyAV CCTTC 3 cut(s) 202, 229, 559
HpyCH4III ACNGT 2 cut(s) 184, 518
HpyCH4V TGCA 1 cut(s) 744
HpyF10VI GCNNNNNNNGC 7 cut(s) 114, 281, 541, 596, 649, 871, 941
HpyF3I CTNAG 5 cut(s) 364, 653, 694, 770, 920
Hsp92I GRCGYC 1 cut(s) 813
Hsp92II CATG 1 cut(s) 113
KroI GCCGGC 1 cut(s) 809
KroNI GCCGGC 1 cut(s) 811
Kzo9I GATC 7 cut(s) 57, 99, 490, 564, 570, 697, 721
LmnI GCTCC 5 cut(s) 89, 206, 212, 434, 884
Lsp1109I GCAGC 2 cut(s) 721, 919
MaeI CTAG 3 cut(s) 17, 621, 936
MaeIII GTNAC 2 cut(s) 184, 484
MalI GATC 7 cut(s) 59, 101, 492, 566, 572, 699, 723
MboI GATC 7 cut(s) 57, 99, 490, 564, 570, 697, 721
MboII GAAGA 4 cut(s) 17, 94, 187, 463
MhlI GDGCHC 4 cut(s) 431, 588, 601, 881
MluCI AATT 5 cut(s) 151, 359, 610, 636, 799
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 4 cut(s) 85, 396, 411, 742
MreI CGCCGGCG 1 cut(s) 809
MroNI GCCGGC 1 cut(s) 809
MspI CCGG 7 cut(s) 204, 234, 238, 321, 482, 532, 810
MspR9I CCNGG 4 cut(s) 47, 81, 239, 862
MvaI CCWGG 3 cut(s) 47, 81, 862
MvnI CGCG 2 cut(s) 590, 797
MwoI GCNNNNNNNGC 7 cut(s) 114, 281, 541, 596, 649, 871, 941
NaeI GCCGGC 1 cut(s) 811
NciI CCSGG 1 cut(s) 239
NdeII GATC 7 cut(s) 57, 99, 490, 564, 570, 697, 721
NgoMIV GCCGGC 1 cut(s) 809
NlaIII CATG 1 cut(s) 113
NlaIV GGNNCC 3 cut(s) 85, 208, 586
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 2 cut(s) 184, 484
PcsI WCGNNNNNNNCGW 1 cut(s) 794
PdiI GCCGGC 1 cut(s) 811
PkrI GCNGC 7 cut(s) 263, 326, 436, 439, 711, 796, 934
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp124BI GAGCTC 2 cut(s) 431, 881
Psp6I CCWGG 3 cut(s) 45, 79, 860
PspEI GGTNACC 1 cut(s) 484
PspFI CCCAGC 2 cut(s) 40, 276
PspGI CCWGG 3 cut(s) 45, 79, 860
PspN4I GGNNCC 3 cut(s) 85, 208, 586
PspOMI GGGCCC 1 cut(s) 584
PspPI GGNCC 4 cut(s) 38, 283, 584, 585
RsaI GTAC 2 cut(s) 515, 926
RsaNI GTAC 2 cut(s) 514, 925
SacI GAGCTC 2 cut(s) 431, 881
SalI GTCGAC 3 cut(s) 89, 242, 674
SatI GCNGC 7 cut(s) 262, 325, 435, 438, 710, 795, 933
Sau3AI GATC 7 cut(s) 57, 99, 490, 564, 570, 697, 721
Sau96I GGNCC 4 cut(s) 38, 283, 584, 585
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 4 cut(s) 47, 81, 239, 862
SduI GDGCHC 4 cut(s) 431, 588, 601, 881
SgrAI CRCCGGYG 1 cut(s) 809
SgrDI CGTCGACG 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 704, 893
SmoI CTYRAG 2 cut(s) 704, 893
Sse9I AATT 5 cut(s) 151, 359, 610, 636, 799
SspMI CTAG 3 cut(s) 17, 621, 936
SstI GAGCTC 2 cut(s) 431, 881
StyD4I CCNGG 4 cut(s) 45, 79, 237, 860
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 2 cut(s) 184, 518
TaqI TCGA 6 cut(s) 90, 243, 552, 563, 675, 816
TasI AATT 5 cut(s) 151, 359, 610, 636, 799
TauI GCSGC 5 cut(s) 264, 327, 437, 440, 797
TscAI CASTG 1 cut(s) 751
TseFI GTSAC 2 cut(s) 184, 484
TseI GCWGC 2 cut(s) 709, 932
Tsp45I GTSAC 2 cut(s) 184, 484
TspDTI ATGAA 1 cut(s) 683
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 1 cut(s) 751
XagI CCTNNNNNAGG 1 cut(s) 20
XapI RAATTY 2 cut(s) 359, 610
XmiI GTMKAC 3 cut(s) 90, 243, 675
XspI CTAG 3 cut(s) 17, 621, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.