pycom04g17670

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0008545
Physical Location & Seq
Reverse (-)
19972184 .. 19975684
3501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGGCGGACGAAGAAGAAGCAGAGCTAAGCGAGAAGCAGAAGAAGGAGATAGCAAAGTGGTTCCTCCTCAACTCTCCCCCCGGAGAAATCCAATTCGTCGCCAAAGGTCTCACCTTTCACCCCAAAAATGTGAAGGCGGTAATCAACGACGACGTTTTGTACGGGGAGGCTGCGTCAGAGGCCTTCCCACTGTACAACAAATCCCACTTGATTTCCATCGAAATGCCGGGCCGAATCGGCGACGTTATAGTTACATCATATGGTGAGCTCGGTGAGACCGAGTTCATTGATCCCAGGACAGCTCAAGTTGCTGTAGTTGACCACATTAAACAGGTTTGTACAGAGGTGAGACCTGCACTGGATGAGGAACTTCCATCCGCTTATGTTGAGGAATTCCGGTGTGCTCTGGATGCAGAAATACTTAAATATGTTGGTGAAGCTTATCCGAAAGGAGTCTGCTCAGTGTACTGTGGCAATGGTAAAGATATGGAGGGCCCTGGATTTGACTTTGAGCTTGTAGTGGTGATTTCAGCTGCTAGACACAGCCCACAAAATTTCTGTAACGGTAGTTGGCGTTCTGTATGGAGCATTGAGTTCAAAGATGAAATACAAATGCTGGAATTAAAAGGCAAACTGCAGGTAGGTGCCCACTATTTTGAGGAGGGCAATGTGCAGTTAGATGCAAAACATGAATGCAAAGATACAACAATTTTTCAGTCCTCTGAAGATAGTGCAATTGCCATTAGCAACATTATTCGTCAACACGAGACTGAGTACTTAACATCTCTTGAGGCATCTTATTCAAATTTGCCTGATACCACTTTCAAGGATCTTCGAAGAAAGCTTCCAGTTACGCGCACCCTATTTCCGTGGCAGAATACCTTGCAGTTCAGCTTGACGAGAGACATCACCAAAGAACTTGGCATCGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

312

Amino Acids

34.97

Weight (kDa)

4.86

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 18 - 302 4.9e-76 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 361, 628
AccB1I GGYRCC 1 cut(s) 644
AccII CGCG 1 cut(s) 856
AciI CCGC 3 cut(s) 5, 137, 378
AclWI GGATC 2 cut(s) 284, 837
AcsI RAATTY 3 cut(s) 392, 553, 805
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 5 cut(s) 161, 194, 340, 467, 776
AgsI TTSAA 3 cut(s) 598, 804, 826
AjnI CCWGG 2 cut(s) 293, 496
AluBI AGCT 8 cut(s) 25, 268, 302, 440, 514, 533, 844, 894
AluI AGCT 8 cut(s) 25, 268, 302, 440, 514, 533, 844, 894
Alw21I GWGCWC 2 cut(s) 270, 406
Alw26I GTCTC 5 cut(s) 113, 269, 343, 761, 897
AlwI GGATC 2 cut(s) 284, 837
AoxI GGCC 3 cut(s) 180, 229, 493
ApaI GGGCCC 1 cut(s) 497
ApeKI GCWGC 2 cut(s) 170, 533
ApoI RAATTY 3 cut(s) 392, 553, 805
AspLEI GCGC 1 cut(s) 858
AspS9I GGNCC 3 cut(s) 229, 493, 494
AsuC2I CCSGG 2 cut(s) 81, 228
AsuHPI GGTGA 8 cut(s) 103, 110, 275, 284, 358, 446, 535, 901
AsuII TTCGAA 1 cut(s) 835
BaeGI GKGCMC 2 cut(s) 497, 649
BanI GGYRCC 1 cut(s) 644
BanII GRGCYC 2 cut(s) 270, 497
BauI CACGAG 1 cut(s) 764
Bbv12I GWGCWC 2 cut(s) 270, 406
BbvI GCAGC 2 cut(s) 157, 520
BccI CCATC 2 cut(s) 224, 382
BciT130I CCWGG 2 cut(s) 295, 498
BcnI CCSGG 2 cut(s) 81, 228
BcoDI GTCTC 5 cut(s) 113, 269, 343, 761, 897
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 2 cut(s) 312, 635
BfuAI ACCTGC 2 cut(s) 361, 628
BglI GCCNNNNNGGC 1 cut(s) 237
BisI GCNGC 2 cut(s) 171, 534
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 2 cut(s) 172, 535
BmcAI AGTACT 1 cut(s) 776
Bme1390I CCNGG 4 cut(s) 81, 228, 295, 498
BmgT120I GGNCC 3 cut(s) 229, 493, 494
BmiI GGNNCC 3 cut(s) 62, 495, 646
BmrFI CCNGG 4 cut(s) 81, 228, 295, 498
BmsI GCATC 3 cut(s) 400, 670, 803
Bpu1102I GCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 835
BpuEI CTTGAG 2 cut(s) 288, 809
BpuMI CCSGG 2 cut(s) 81, 228
BsaBI GATNNNNATC 1 cut(s) 215
BsaI GGTCTC 3 cut(s) 113, 269, 343
BsaJI CCNNGG 4 cut(s) 79, 293, 496, 869
BsaWI WCCGGW 1 cut(s) 396
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bse1I ACTGG 2 cut(s) 363, 848
Bse3DI GCAATG 2 cut(s) 481, 673
Bse8I GATNNNNATC 1 cut(s) 215
BseBI CCWGG 2 cut(s) 295, 498
BseDI CCNNGG 4 cut(s) 79, 293, 496, 869
BseGI GGATG 3 cut(s) 367, 374, 415
BseJI GATNNNNATC 1 cut(s) 215
BseMI GCAATG 2 cut(s) 481, 673
BseMII CTCAG 2 cut(s) 474, 762
BseNI ACTGG 2 cut(s) 363, 848
BseRI GAGGAG 2 cut(s) 56, 674
BseSI GKGCMC 2 cut(s) 497, 649
BseXI GCAGC 2 cut(s) 157, 520
BsgI GTGCAG 2 cut(s) 339, 692
Bsh1236I CGCG 1 cut(s) 856
BshFI GGCC 3 cut(s) 182, 231, 495
BshNI GGYRCC 1 cut(s) 644
BsiHKAI GWGCWC 2 cut(s) 270, 406
BsiSI CCGG 3 cut(s) 81, 227, 397
BsmAI GTCTC 5 cut(s) 113, 269, 343, 761, 897
BsmI GAATGC 1 cut(s) 698
BsnI GGCC 3 cut(s) 182, 231, 495
Bso31I GGTCTC 3 cut(s) 113, 269, 343
Bsp119I TTCGAA 1 cut(s) 835
Bsp120I GGGCCC 1 cut(s) 493
Bsp1286I GDGCHC 4 cut(s) 270, 406, 497, 649
Bsp1407I TGTACA 2 cut(s) 192, 338
Bsp143I GATC 2 cut(s) 289, 829
Bsp1720I GCTNAGC 1 cut(s) 26
BspACI CCGC 3 cut(s) 5, 137, 378
BspANI GGCC 3 cut(s) 182, 231, 495
BspCNI CTCAG 2 cut(s) 473, 763
BspFNI CGCG 1 cut(s) 856
BspLI GGNNCC 3 cut(s) 62, 495, 646
BspMAI CTGCAG 1 cut(s) 639
BspMI ACCTGC 2 cut(s) 361, 628
BspPI GGATC 2 cut(s) 284, 837
BspT104I TTCGAA 1 cut(s) 835
BspT107I GGYRCC 1 cut(s) 644
BspTNI GGTCTC 3 cut(s) 113, 269, 343
BsrDI GCAATG 2 cut(s) 481, 673
BsrGI TGTACA 2 cut(s) 192, 338
BsrI ACTGG 2 cut(s) 363, 848
BssECI CCNNGG 4 cut(s) 79, 293, 496, 869
BssMI GATC 2 cut(s) 289, 829
BssSI CACGAG 1 cut(s) 764
Bst2BI CACGAG 1 cut(s) 764
Bst2UI CCWGG 2 cut(s) 295, 498
Bst4CI ACNGT 3 cut(s) 192, 470, 566
BstAUI TGTACA 2 cut(s) 192, 338
BstBI TTCGAA 1 cut(s) 835
BstDEI CTNAG 3 cut(s) 26, 460, 771
BstDSI CCRYGG 1 cut(s) 869
BstF5I GGATG 3 cut(s) 367, 374, 415
BstFNI CGCG 1 cut(s) 856
BstHHI GCGC 1 cut(s) 858
BstKTI GATC 2 cut(s) 292, 832
BstMAI GTCTC 5 cut(s) 113, 269, 343, 761, 897
BstMBI GATC 2 cut(s) 289, 829
BstMWI GCNNNNNNNGC 4 cut(s) 179, 237, 308, 410
BstNI CCWGG 2 cut(s) 295, 498
BstSCI CCNGG 4 cut(s) 79, 226, 293, 496
BstSFI CTRYAG 2 cut(s) 312, 635
BstSLI GKGCMC 2 cut(s) 497, 649
BstUI CGCG 1 cut(s) 856
BstV1I GCAGC 2 cut(s) 157, 520
BstX2I RGATCY 1 cut(s) 829
BstYI RGATCY 1 cut(s) 829
BsuRI GGCC 3 cut(s) 182, 231, 495
BtgI CCRYGG 1 cut(s) 869
BtsCI GGATG 3 cut(s) 367, 374, 415
BtsIMutI CAGTG 3 cut(s) 188, 356, 468
BveI ACCTGC 2 cut(s) 361, 628
CfoI GCGC 1 cut(s) 858
Cfr13I GGNCC 3 cut(s) 229, 493, 494
CseI GACGC 1 cut(s) 162
Csp6I GTAC 5 cut(s) 160, 193, 339, 466, 775
CviAII CATG 1 cut(s) 689
CviQI GTAC 5 cut(s) 160, 193, 339, 466, 775
DdeI CTNAG 3 cut(s) 26, 460, 771
DpnI GATC 2 cut(s) 291, 831
DpnII GATC 2 cut(s) 289, 829
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 268
Eco147I AGGCCT 1 cut(s) 182
Eco24I GRGCYC 2 cut(s) 270, 497
Eco31I GGTCTC 3 cut(s) 113, 269, 343
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 744
EcoICRI GAGCTC 1 cut(s) 268
EcoO109I RGGNCCY 2 cut(s) 493, 494
EcoRI GAATTC 1 cut(s) 392
EcoRII CCWGG 2 cut(s) 293, 496
EcoT38I GRGCYC 2 cut(s) 270, 497
FaeI CATG 1 cut(s) 692
FaiI YATR 8 cut(s) 248, 259, 261, 384, 429, 488, 583, 690
FatI CATG 1 cut(s) 688
FauNDI CATATG 1 cut(s) 259
Fnu4HI GCNGC 2 cut(s) 171, 534
FokI GGATG 3 cut(s) 361, 374, 422
FriOI GRGCYC 2 cut(s) 270, 497
Fsp4HI GCNGC 2 cut(s) 171, 534
FspBI CTAG 1 cut(s) 537
GlaI GCGC 1 cut(s) 857
GluI GCNGC 2 cut(s) 171, 534
HaeIII GGCC 3 cut(s) 182, 231, 495
HapII CCGG 3 cut(s) 81, 227, 397
HgaI GACGC 1 cut(s) 162
HhaI GCGC 1 cut(s) 858
Hin1II CATG 1 cut(s) 692
Hin6I GCGC 1 cut(s) 856
HinP1I GCGC 1 cut(s) 856
HincII GTYRAC 2 cut(s) 319, 761
HindII GTYRAC 2 cut(s) 319, 761
HindIII AAGCTT 2 cut(s) 438, 842
HinfI GANTC 2 cut(s) 234, 453
HpaII CCGG 3 cut(s) 81, 227, 397
HphI GGTGA 8 cut(s) 103, 110, 275, 284, 358, 446, 535, 901
Hpy166II GTNNAC 3 cut(s) 319, 466, 761
Hpy188I TCNGA 4 cut(s) 178, 447, 724, 929
Hpy188III TCNNGA 2 cut(s) 407, 788
Hpy8I GTNNAC 3 cut(s) 319, 466, 761
Hpy99I CGWCG 4 cut(s) 101, 152, 155, 245
HpyAV CCTTC 3 cut(s) 37, 127, 193
HpyCH4III ACNGT 3 cut(s) 192, 470, 566
HpyCH4IV ACGT 2 cut(s) 153, 243
HpyCH4V TGCA 8 cut(s) 356, 413, 637, 673, 683, 696, 734, 886
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 237, 308, 410
HpyF3I CTNAG 3 cut(s) 26, 460, 771
HpySE526I ACGT 2 cut(s) 153, 243
Hsp92II CATG 1 cut(s) 692
HspAI GCGC 1 cut(s) 856
Kzo9I GATC 2 cut(s) 289, 829
LmnI GCTCC 1 cut(s) 585
Lsp1109I GCAGC 2 cut(s) 157, 520
LweI GCATC 3 cut(s) 400, 670, 803
MaeI CTAG 1 cut(s) 537
MaeII ACGT 2 cut(s) 153, 243
MaeIII GTNAC 3 cut(s) 250, 560, 850
MalI GATC 2 cut(s) 291, 831
MboI GATC 2 cut(s) 289, 829
MboII GAAGA 6 cut(s) 23, 26, 52, 737, 824, 849
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 829
MhlI GDGCHC 4 cut(s) 270, 406, 497, 649
MluCI AATT 7 cut(s) 92, 392, 553, 620, 708, 735, 805
MlyI GAGTC 1 cut(s) 462
MseI TTAA 4 cut(s) 327, 423, 623, 779
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 3 cut(s) 81, 227, 397
MspR9I CCNGG 4 cut(s) 81, 228, 295, 498
MunI CAATTG 1 cut(s) 735
Mva1269I GAATGC 1 cut(s) 698
MvaI CCWGG 2 cut(s) 295, 498
MvnI CGCG 1 cut(s) 856
MwoI GCNNNNNNNGC 4 cut(s) 179, 237, 308, 410
NciI CCSGG 2 cut(s) 81, 228
NdeI CATATG 1 cut(s) 259
NdeII GATC 2 cut(s) 289, 829
NlaIII CATG 1 cut(s) 692
NlaIV GGNNCC 3 cut(s) 62, 495, 646
NspV TTCGAA 1 cut(s) 835
PceI AGGCCT 1 cut(s) 182
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 698
PfeI GAWTC 1 cut(s) 234
PkrI GCNGC 2 cut(s) 172, 535
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 2 cut(s) 293, 496
PspGI CCWGG 2 cut(s) 293, 496
PspN4I GGNNCC 3 cut(s) 62, 495, 646
PspOMI GGGCCC 1 cut(s) 493
PspPI GGNCC 3 cut(s) 229, 493, 494
PsrI GAACNNNNNNTAC 2 cut(s) 559, 591
PstI CTGCAG 1 cut(s) 639
PsuI RGATCY 1 cut(s) 829
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 5 cut(s) 161, 194, 340, 467, 776
RsaNI GTAC 5 cut(s) 160, 193, 339, 466, 775
RseI CAYNNNNRTG 1 cut(s) 221
SacI GAGCTC 1 cut(s) 270
SaqAI TTAA 4 cut(s) 327, 423, 623, 779
SatI GCNGC 2 cut(s) 171, 534
Sau3AI GATC 2 cut(s) 289, 829
Sau96I GGNCC 3 cut(s) 229, 493, 494
ScaI AGTACT 1 cut(s) 776
SchI GAGTC 1 cut(s) 462
ScrFI CCNGG 4 cut(s) 81, 228, 295, 498
SduI GDGCHC 4 cut(s) 270, 406, 497, 649
SfaNI GCATC 3 cut(s) 400, 670, 803
SfcI CTRYAG 2 cut(s) 312, 635
SfuI TTCGAA 1 cut(s) 835
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 2 cut(s) 303, 788
SmoI CTYRAG 2 cut(s) 303, 788
Sse9I AATT 7 cut(s) 92, 392, 553, 620, 708, 735, 805
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 3 cut(s) 5, 137, 378
SspMI CTAG 1 cut(s) 537
SstI GAGCTC 1 cut(s) 270
StuI AGGCCT 1 cut(s) 182
StyD4I CCNGG 4 cut(s) 79, 226, 293, 496
TaaI ACNGT 3 cut(s) 192, 470, 566
TaiI ACGT 2 cut(s) 156, 246
TaqI TCGA 2 cut(s) 219, 835
TaqII GACCGA 1 cut(s) 293
TasI AATT 7 cut(s) 92, 392, 553, 620, 708, 735, 805
TatI WGTACW 4 cut(s) 192, 338, 465, 774
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 4 cut(s) 327, 423, 623, 779
Tru9I TTAA 4 cut(s) 327, 423, 623, 779
TscAI CASTG 3 cut(s) 195, 363, 468
TseI GCWGC 2 cut(s) 170, 533
TspDTI ATGAA 3 cut(s) 274, 618, 705
TspGWI ACGGA 1 cut(s) 858
TspRI CASTG 3 cut(s) 195, 363, 468
XapI RAATTY 3 cut(s) 392, 553, 805
XspI CTAG 1 cut(s) 537
ZrmI AGTACT 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.