pycom04g21510

GDT1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009329
Physical Location & Seq
Forward (+)
22641928 .. 22644505
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
ATGGGGTTTCGCTCGATTCCCAGAACGATTGCGTACTCCCTCCCCCTCCTCTTGCTCCTCCTCCTTGCGGTTTCTGCTCAGGAGTCTGGGGCTGAAGCTGAGAGGGGAGGCTATGGGGGATCCAAAGATCTGGGCCGTCGTAGCAAGATAAAATTTGGTATTCTTGAAGTTAATACTCTTGGAGTCAAGACAGAGGGTGACTCAGTTGCTATCGGCCTCCACGTGGACTCAGGTCTTGGAGTTTTCGATGCCTTTGTTGCAAGCTTGTCAATGATACTAGTTAGTGAGATTGGAGACGAAACATTTATAATAGCAGCTCTTATGGCAATGCGTCACCCCAAGTCAATTGTTCTATCTGGTGCACTTTCAGCCTTGTTTATAATGACGGTACTTTCCACTGGACTTGGTAGGATTGTGCCAAACTTGATATCAAGGAAGCATACAAATAGTGCAGCTACAGTTCTTTATTTATTTTTTGGACTGAGGTTACTTTACATTGCTTGGATCGATCATCTGATTCTAAATCTTCCCAGAAGAAAGAAATTGAAGAAGTTGAAGAGAAACTTGAGTCTGGTCAGGGAAAGACACCCTTCCGCCGTTTCTTTTCCAGATTTTGTACACCTATCTTCTTGGAGTCATTCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0016

Protein Analysis

216

Amino Acids

23.48

Weight (kDa)

10.15

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDT1 PF01169 87 - 160 2.5e-18 Divalent cation/proton antiporter GDT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 308, 380
AciI CCGC 2 cut(s) 68, 594
AclWI GGATC 3 cut(s) 114, 127, 512
AcsI RAATTY 1 cut(s) 152
AcuI CTGAAG 1 cut(s) 114
AcvI CACGTG 1 cut(s) 223
AfaI GTAC 3 cut(s) 35, 390, 618
AfiI CCNNNNNNNGG 2 cut(s) 67, 223
AgsI TTSAA 3 cut(s) 167, 547, 556
AhlI ACTAGT 1 cut(s) 277
AluBI AGCT 4 cut(s) 98, 264, 317, 455
AluI AGCT 4 cut(s) 98, 264, 317, 455
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 1 cut(s) 288
Alw44I GTGCAC 1 cut(s) 360
AlwI GGATC 3 cut(s) 114, 127, 512
AoxI GGCC 2 cut(s) 133, 214
ApaLI GTGCAC 1 cut(s) 360
ApeKI GCWGC 2 cut(s) 314, 452
ApoI RAATTY 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 2 cut(s) 209, 326
BaeGI GKGCMC 1 cut(s) 364
BamHI GGATCC 1 cut(s) 119
BbrPI CACGTG 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 2 cut(s) 326, 464
BceAI ACGGC 2 cut(s) 120, 581
BcoDI GTCTC 1 cut(s) 288
BcuI ACTAGT 1 cut(s) 277
BfaI CTAG 1 cut(s) 278
BfmI CTRYAG 1 cut(s) 456
BglII AGATCT 1 cut(s) 127
BisI GCNGC 2 cut(s) 315, 453
BlsI GCNGC 2 cut(s) 316, 454
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 121
BmsI GCATC 1 cut(s) 238
BoxI GACNNNNGTC 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 185, 217
Bpu10I CCTNAGC 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 586
Bsa29I ATCGAT 1 cut(s) 507
BsaAI YACGTR 1 cut(s) 223
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 223
Bse1I ACTGG 1 cut(s) 403
Bse3DI GCAATG 2 cut(s) 333, 495
BseCI ATCGAT 1 cut(s) 507
BseLI CCNNNNNNNGG 2 cut(s) 67, 223
BseMI GCAATG 2 cut(s) 333, 495
BseMII CTCAG 5 cut(s) 90, 92, 216, 243, 473
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 3 cut(s) 38, 47, 50
BseSI GKGCMC 1 cut(s) 364
BseXI GCAGC 2 cut(s) 326, 464
BsgI GTGCAG 1 cut(s) 471
BshFI GGCC 2 cut(s) 135, 216
BshVI ATCGAT 1 cut(s) 507
BsiHKAI GWGCWC 1 cut(s) 364
BslI CCNNNNNNNGG 2 cut(s) 67, 223
BsmAI GTCTC 1 cut(s) 288
BsmBI CGTCTC 1 cut(s) 288
BsnI GGCC 2 cut(s) 135, 216
Bsp1286I GDGCHC 1 cut(s) 364
Bsp1407I TGTACA 1 cut(s) 616
Bsp143I GATC 4 cut(s) 119, 127, 504, 508
BspACI CCGC 2 cut(s) 68, 594
BspANI GGCC 2 cut(s) 135, 216
BspCNI CTCAG 5 cut(s) 91, 91, 215, 242, 474
BspDI ATCGAT 1 cut(s) 507
BspLI GGNNCC 1 cut(s) 121
BspPI GGATC 3 cut(s) 114, 127, 512
BsrDI GCAATG 2 cut(s) 333, 495
BsrGI TGTACA 1 cut(s) 616
BsrI ACTGG 1 cut(s) 403
BssMI GATC 4 cut(s) 119, 127, 504, 508
Bst4CI ACNGT 2 cut(s) 388, 460
Bst6I CTCTTC 1 cut(s) 551
BstAUI TGTACA 1 cut(s) 616
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 5 cut(s) 78, 99, 202, 229, 482
BstKTI GATC 4 cut(s) 122, 130, 507, 511
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 4 cut(s) 119, 127, 504, 508
BstMWI GCNNNNNNNGC 5 cut(s) 74, 141, 257, 323, 368
BstPAI GACNNNNGTC 1 cut(s) 231
BstSFI CTRYAG 1 cut(s) 456
BstSLI GKGCMC 1 cut(s) 364
BstV1I GCAGC 2 cut(s) 326, 464
BstX2I RGATCY 2 cut(s) 119, 127
BstXI CCANNNNNNTGG 1 cut(s) 130
BstYI RGATCY 2 cut(s) 119, 127
Bsu15I ATCGAT 1 cut(s) 507
BsuRI GGCC 2 cut(s) 135, 216
BsuTUI ATCGAT 1 cut(s) 507
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 1 cut(s) 262
Cfr13I GGNCC 1 cut(s) 133
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 1 cut(s) 320
Csp6I GTAC 3 cut(s) 34, 389, 617
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviJI RGCY 9 cut(s) 92, 98, 111, 135, 216, 264, 317, 371, 455
CviKI_1 RGCY 9 cut(s) 92, 98, 111, 135, 216, 264, 317, 371, 455
CviQI GTAC 3 cut(s) 34, 389, 617
DdeI CTNAG 5 cut(s) 78, 99, 202, 229, 482
DpnI GATC 4 cut(s) 121, 129, 506, 510
DpnII GATC 4 cut(s) 119, 127, 504, 508
Eam1104I CTCTTC 1 cut(s) 551
EarI CTCTTC 1 cut(s) 551
EciI GGCGGA 1 cut(s) 583
Eco32I GATATC 1 cut(s) 429
Eco57I CTGAAG 1 cut(s) 114
Eco72I CACGTG 1 cut(s) 223
EcoRV GATATC 1 cut(s) 429
Esp3I CGTCTC 1 cut(s) 288
FaiI YATR 5 cut(s) 114, 308, 323, 380, 441
FalI AAGNNNNNCTT 4 cut(s) 548, 580, 574, 606
Fnu4HI GCNGC 2 cut(s) 315, 453
Fsp4HI GCNGC 2 cut(s) 315, 453
FspBI CTAG 1 cut(s) 278
GluI GCNGC 2 cut(s) 315, 453
HaeIII GGCC 2 cut(s) 135, 216
HgaI GACGC 1 cut(s) 320
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 8 cut(s) 16, 83, 183, 200, 227, 517, 568, 634
HphI GGTGA 2 cut(s) 209, 326
Hpy166II GTNNAC 3 cut(s) 226, 362, 619
Hpy188I TCNGA 1 cut(s) 516
Hpy188III TCNNGA 4 cut(s) 80, 164, 187, 608
Hpy8I GTNNAC 3 cut(s) 226, 362, 619
Hpy99I CGWCG 1 cut(s) 141
HpyAV CCTTC 1 cut(s) 600
HpyCH4III ACNGT 2 cut(s) 388, 460
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 3 cut(s) 260, 362, 452
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 141, 257, 323, 368
HpyF3I CTNAG 5 cut(s) 78, 99, 202, 229, 482
HpySE526I ACGT 1 cut(s) 222
Kzo9I GATC 4 cut(s) 119, 127, 504, 508
LmnI GCTCC 1 cut(s) 60
Lsp1109I GCAGC 2 cut(s) 326, 464
LweI GCATC 1 cut(s) 238
MaeI CTAG 1 cut(s) 278
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 3 cut(s) 197, 332, 486
MalI GATC 4 cut(s) 121, 129, 506, 510
MboI GATC 4 cut(s) 119, 127, 504, 508
MboII GAAGA 5 cut(s) 518, 546, 559, 568, 618
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 2 cut(s) 119, 127
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 3 cut(s) 152, 345, 542
MlyI GAGTC 6 cut(s) 92, 192, 194, 221, 577, 643
MseI TTAA 2 cut(s) 171, 646
MunI CAATTG 1 cut(s) 345
MwoI GCNNNNNNNGC 5 cut(s) 74, 141, 257, 323, 368
NdeII GATC 4 cut(s) 119, 127, 504, 508
NlaIV GGNNCC 1 cut(s) 121
NmuCI GTSAC 2 cut(s) 197, 332
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 2 cut(s) 16, 517
PkrI GCNGC 2 cut(s) 316, 454
PleI GAGTC 6 cut(s) 91, 191, 194, 221, 576, 642
PmaCI CACGTG 1 cut(s) 223
PmlI CACGTG 1 cut(s) 223
PpsI GAGTC 6 cut(s) 91, 191, 194, 221, 576, 642
Ppu21I YACGTR 1 cut(s) 223
PshAI GACNNNNGTC 1 cut(s) 231
PsiI TTATAA 2 cut(s) 308, 380
PspCI CACGTG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 2 cut(s) 119, 127
RsaI GTAC 3 cut(s) 35, 390, 618
RsaNI GTAC 3 cut(s) 34, 389, 617
SaqAI TTAA 2 cut(s) 171, 646
SatI GCNGC 2 cut(s) 315, 453
Sau3AI GATC 4 cut(s) 119, 127, 504, 508
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 6 cut(s) 92, 192, 194, 221, 577, 643
SduI GDGCHC 1 cut(s) 364
SetI ASST 8 cut(s) 100, 225, 235, 266, 319, 457, 488, 624
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 565
SmoI CTYRAG 1 cut(s) 565
SpeI ACTAGT 1 cut(s) 277
Sse9I AATT 3 cut(s) 152, 345, 542
SsiI CCGC 2 cut(s) 68, 594
SspMI CTAG 1 cut(s) 278
TaaI ACNGT 2 cut(s) 388, 460
TaiI ACGT 1 cut(s) 225
TaqI TCGA 3 cut(s) 14, 246, 507
TasI AATT 3 cut(s) 152, 345, 542
TatI WGTACW 1 cut(s) 616
TfiI GAWTC 2 cut(s) 16, 517
Tru1I TTAA 2 cut(s) 171, 646
Tru9I TTAA 2 cut(s) 171, 646
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 2 cut(s) 197, 332
TseI GCWGC 2 cut(s) 314, 452
Tsp45I GTSAC 2 cut(s) 197, 332
TspDTI ATGAA 1 cut(s) 630
TspRI CASTG 1 cut(s) 403
VneI GTGCAC 1 cut(s) 360
XapI RAATTY 1 cut(s) 152
XspI CTAG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.