pycom12g23810

GDT1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009329
Physical Location & Seq
Forward (+)
24288781 .. 24291728
2948 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23810.8

Sequence Viewer

Length: 882 bp
ATGGGGTGTCGCTCGATTCCCAGAACGATTGCGTACTCCCTCCCCCTCCTATTGCTCCTTCTCCTCGCCGTTTCTGCTCAGGAGTCCGGGGTTGAAGCTGAGAGGGGAGACTCCAGGGGATCCAAAGATCTGGGCCGTCGGAGCAAGATTAAATTGGGTATTCTTGAAGTTAATACTCTTGGCGTCAAGACAGAGGGTGACTCAGTTGCTATTGGTCTCCACGTGGACTCAGGTCTTGGAATTTTTGATGCCTTTATTGCAAGCTTGTCAATGATACTAGTCAGTGAGATCGGAGATGAAACCTTTATAATAGCAGCTCTTATGGCAATGCGTCACCCCAGGTCAATTGTCCTATCTGGTGCACTTTCAGCCTTGTTTATAATGACGGTACTTTCCACTGGACTTGGTAGGATTGTGCCAAACTTGATATCAAGGAAGCATACGAATAGTGCAGCTACAGTTCTGTATTTATTTTTTGGACTGAGGTTACTTTACATTGCTTGGAGATCATCAGATTCTAAATCTTCCCAGAAGAAAGAAATTGAAGAAGTTGAAGAGAAACTTGAGTCTGGACAGGGAAAAACACCCTTCCGCCGTTTCTTTTCCAGATTTTTAACACCCATCTTCTTGGAGTCATTCATTTTAACGTTTTTAGCAGAGTGGGGAGATCGCAGCCAAATAGCGACAATTGCTCTGGCAACCCATAAAAATGCAGTTGGAGTTGCGGTGGGTGCAACACTAGGACATACAATCTGCACGTCTGTGGCTGTGGTAGGAGGGAGTATGTTGGCATCCAAAATCTCCCAACGGACAGTCGCAACAGTTGGAGGCCTGCTCTTCCTATGCTTCTCCGTGTCGTCTTACTTCTATCCTCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0016

Protein Analysis

294

Amino Acids

31.52

Weight (kDa)

9.32

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 308, 380
AciI CCGC 2 cut(s) 592, 725
AclI AACGTT 1 cut(s) 647
AclWI GGATC 2 cut(s) 114, 127
AcsI RAATTY 1 cut(s) 240
AcvI CACGTG 1 cut(s) 223
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 2 cut(s) 35, 390
AgsI TTSAA 4 cut(s) 95, 167, 545, 554
AhlI ACTAGT 1 cut(s) 277
AjiI CACGTC 1 cut(s) 759
AjnI CCWGG 2 cut(s) 113, 338
AleI CACNNNNGTG 1 cut(s) 761
AluBI AGCT 4 cut(s) 98, 264, 317, 455
AluI AGCT 4 cut(s) 98, 264, 317, 455
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 2 cut(s) 102, 221
Alw44I GTGCAC 1 cut(s) 360
AlwI GGATC 2 cut(s) 114, 127
AoxI GGCC 2 cut(s) 133, 829
ApaLI GTGCAC 1 cut(s) 360
ApeKI GCWGC 3 cut(s) 314, 452, 672
ApoI RAATTY 1 cut(s) 240
AspS9I GGNCC 1 cut(s) 133
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 209, 326
BaeGI GKGCMC 1 cut(s) 364
BamHI GGATCC 1 cut(s) 119
BbrPI CACGTG 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 3 cut(s) 326, 464, 684
BccI CCATC 1 cut(s) 629
BceAI ACGGC 3 cut(s) 53, 120, 579
BciT130I CCWGG 2 cut(s) 115, 340
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 2 cut(s) 102, 221
BcuI ACTAGT 1 cut(s) 277
BfaI CTAG 2 cut(s) 278, 740
BfmI CTRYAG 1 cut(s) 456
BglII AGATCT 1 cut(s) 127
BisI GCNGC 3 cut(s) 315, 453, 673
BlsI GCNGC 3 cut(s) 316, 454, 674
Bme1390I CCNGG 3 cut(s) 88, 115, 340
BmgBI CACGTC 1 cut(s) 759
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 3 cut(s) 88, 115, 340
BmsI GCATC 2 cut(s) 238, 800
BoxI GACNNNNGTC 1 cut(s) 231
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 819, 851
BpmI CTGGAG 1 cut(s) 97
Bpu10I CCTNAGC 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 1 cut(s) 88
BsaAI YACGTR 1 cut(s) 223
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 3 cut(s) 87, 114, 338
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bse1I ACTGG 1 cut(s) 403
Bse3DI GCAATG 2 cut(s) 333, 495
BseBI CCWGG 2 cut(s) 115, 340
BseDI CCNNGG 3 cut(s) 87, 114, 338
BseGI GGATG 1 cut(s) 791
BseMI GCAATG 2 cut(s) 333, 495
BseMII CTCAG 5 cut(s) 90, 92, 216, 243, 473
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 2 cut(s) 53, 864
BseSI GKGCMC 1 cut(s) 364
BseXI GCAGC 3 cut(s) 326, 464, 684
BsgI GTGCAG 2 cut(s) 471, 739
BshFI GGCC 2 cut(s) 135, 831
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 1 cut(s) 87
BsmAI GTCTC 2 cut(s) 102, 221
BsnI GGCC 2 cut(s) 135, 831
Bso31I GGTCTC 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 5 cut(s) 119, 127, 288, 506, 667
BspACI CCGC 2 cut(s) 592, 725
BspANI GGCC 2 cut(s) 135, 831
BspCNI CTCAG 5 cut(s) 91, 91, 215, 242, 474
BspLI GGNNCC 1 cut(s) 121
BspPI GGATC 2 cut(s) 114, 127
BspQI GCTCTTC 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 2 cut(s) 333, 495
BsrI ACTGG 1 cut(s) 403
BssECI CCNNGG 3 cut(s) 87, 114, 338
BssMI GATC 5 cut(s) 119, 127, 288, 506, 667
BssNI GRCGYC 1 cut(s) 183
Bst2UI CCWGG 2 cut(s) 115, 340
Bst4CI ACNGT 4 cut(s) 388, 460, 814, 823
Bst6I CTCTTC 2 cut(s) 549, 842
BstACI GRCGYC 1 cut(s) 183
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 262, 833
BstDEI CTNAG 5 cut(s) 78, 99, 202, 229, 482
BstF5I GGATG 1 cut(s) 791
BstKTI GATC 5 cut(s) 122, 130, 291, 509, 670
BstMAI GTCTC 2 cut(s) 102, 221
BstMBI GATC 5 cut(s) 119, 127, 288, 506, 667
BstMWI GCNNNNNNNGC 7 cut(s) 74, 141, 257, 323, 368, 689, 731
BstNI CCWGG 2 cut(s) 115, 340
BstPAI GACNNNNGTC 1 cut(s) 231
BstSCI CCNGG 3 cut(s) 86, 113, 338
BstSFI CTRYAG 1 cut(s) 456
BstSLI GKGCMC 1 cut(s) 364
BstV1I GCAGC 3 cut(s) 326, 464, 684
BstX2I RGATCY 2 cut(s) 119, 127
BstXI CCANNNNNNTGG 2 cut(s) 130, 628
BstYI RGATCY 2 cut(s) 119, 127
BsuRI GGCC 2 cut(s) 135, 831
BtrI CACGTC 1 cut(s) 759
BtsCI GGATG 1 cut(s) 791
BtsIMutI CAGTG 2 cut(s) 289, 396
Cac8I GCNNGC 2 cut(s) 262, 833
Cfr13I GGNCC 1 cut(s) 133
CseI GACGC 2 cut(s) 172, 320
Csp6I GTAC 2 cut(s) 34, 389
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviJI RGCY 9 cut(s) 98, 135, 264, 317, 371, 455, 675, 767, 831
CviKI_1 RGCY 9 cut(s) 98, 135, 264, 317, 371, 455, 675, 767, 831
CviQI GTAC 2 cut(s) 34, 389
DdeI CTNAG 5 cut(s) 78, 99, 202, 229, 482
DpnI GATC 5 cut(s) 121, 129, 290, 508, 669
DpnII GATC 5 cut(s) 119, 127, 288, 506, 667
Eam1104I CTCTTC 2 cut(s) 549, 842
EarI CTCTTC 2 cut(s) 549, 842
EciI GGCGGA 1 cut(s) 581
Eco147I AGGCCT 1 cut(s) 831
Eco31I GGTCTC 1 cut(s) 221
Eco32I GATATC 1 cut(s) 429
Eco72I CACGTG 1 cut(s) 223
EcoRII CCWGG 2 cut(s) 113, 338
EcoRV GATATC 1 cut(s) 429
FaiI YATR 8 cut(s) 308, 323, 380, 441, 705, 747, 785, 844
FalI AAGNNNNNCTT 2 cut(s) 546, 578
Fnu4HI GCNGC 3 cut(s) 315, 453, 673
FokI GGATG 1 cut(s) 778
Fsp4HI GCNGC 3 cut(s) 315, 453, 673
FspBI CTAG 2 cut(s) 278, 740
GluI GCNGC 3 cut(s) 315, 453, 673
GsuI CTGGAG 1 cut(s) 97
HaeIII GGCC 2 cut(s) 135, 831
HapII CCGG 1 cut(s) 87
HgaI GACGC 2 cut(s) 172, 320
Hin1I GRCGYC 1 cut(s) 183
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 8 cut(s) 16, 83, 110, 200, 227, 515, 566, 632
HpaII CCGG 1 cut(s) 87
HphI GGTGA 2 cut(s) 209, 326
Hpy166II GTNNAC 2 cut(s) 226, 362
Hpy188I TCNGA 3 cut(s) 141, 293, 514
Hpy188III TCNNGA 5 cut(s) 80, 164, 187, 570, 606
Hpy8I GTNNAC 2 cut(s) 226, 362
Hpy99I CGWCG 1 cut(s) 141
HpyAV CCTTC 2 cut(s) 68, 598
HpyCH4III ACNGT 4 cut(s) 388, 460, 814, 823
HpyCH4IV ACGT 3 cut(s) 222, 647, 758
HpyCH4V TGCA 6 cut(s) 260, 362, 452, 713, 734, 756
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 141, 257, 323, 368, 689, 731
HpyF3I CTNAG 5 cut(s) 78, 99, 202, 229, 482
HpySE526I ACGT 3 cut(s) 222, 647, 758
Hsp92I GRCGYC 1 cut(s) 183
Kzo9I GATC 5 cut(s) 119, 127, 288, 506, 667
LguI GCTCTTC 1 cut(s) 842
LmnI GCTCC 2 cut(s) 60, 141
Lsp1109I GCAGC 3 cut(s) 326, 464, 684
LweI GCATC 2 cut(s) 238, 800
MaeI CTAG 2 cut(s) 278, 740
MaeII ACGT 3 cut(s) 222, 647, 758
MaeIII GTNAC 3 cut(s) 197, 332, 486
MalI GATC 5 cut(s) 121, 129, 290, 508, 669
MboI GATC 5 cut(s) 119, 127, 288, 506, 667
MboII GAAGA 6 cut(s) 516, 544, 557, 566, 616, 829
MfeI CAATTG 2 cut(s) 345, 687
MflI RGATCY 2 cut(s) 119, 127
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 5 cut(s) 152, 240, 345, 540, 687
MlyI GAGTC 6 cut(s) 92, 104, 194, 221, 575, 641
MmeI TCCRAC 3 cut(s) 119, 697, 805
MnlI CCTC 9 cut(s) 50, 56, 74, 96, 187, 477, 770, 821, 882
MseI TTAA 4 cut(s) 150, 171, 614, 644
MslI CAYNNNNRTG 2 cut(s) 708, 761
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 3 cut(s) 88, 115, 340
MunI CAATTG 2 cut(s) 345, 687
MvaI CCWGG 2 cut(s) 115, 340
MwoI GCNNNNNNNGC 7 cut(s) 74, 141, 257, 323, 368, 689, 731
NciI CCSGG 1 cut(s) 88
NdeII GATC 5 cut(s) 119, 127, 288, 506, 667
NlaIV GGNNCC 1 cut(s) 121
NmuCI GTSAC 2 cut(s) 197, 332
OliI CACNNNNGTG 1 cut(s) 761
PceI AGGCCT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 842
PfeI GAWTC 2 cut(s) 16, 515
PkrI GCNGC 3 cut(s) 316, 454, 674
PleI GAGTC 6 cut(s) 91, 104, 194, 221, 574, 640
PmaCI CACGTG 1 cut(s) 223
PmlI CACGTG 1 cut(s) 223
PpsI GAGTC 6 cut(s) 91, 104, 194, 221, 574, 640
Ppu21I YACGTR 1 cut(s) 223
PshAI GACNNNNGTC 1 cut(s) 231
PsiI TTATAA 2 cut(s) 308, 380
Psp1406I AACGTT 1 cut(s) 647
Psp6I CCWGG 2 cut(s) 113, 338
PspCI CACGTG 1 cut(s) 223
PspGI CCWGG 2 cut(s) 113, 338
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 2 cut(s) 119, 127
RsaI GTAC 2 cut(s) 35, 390
RsaNI GTAC 2 cut(s) 34, 389
RseI CAYNNNNRTG 2 cut(s) 708, 761
SapI GCTCTTC 1 cut(s) 842
SaqAI TTAA 4 cut(s) 150, 171, 614, 644
SatI GCNGC 3 cut(s) 315, 453, 673
Sau3AI GATC 5 cut(s) 119, 127, 288, 506, 667
Sau96I GGNCC 1 cut(s) 133
SchI GAGTC 6 cut(s) 92, 104, 194, 221, 575, 641
ScrFI CCNGG 3 cut(s) 88, 115, 340
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 2 cut(s) 238, 800
SfcI CTRYAG 1 cut(s) 456
SmiMI CAYNNNNRTG 2 cut(s) 708, 761
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
SpeI ACTAGT 1 cut(s) 277
Sse9I AATT 5 cut(s) 152, 240, 345, 540, 687
SseBI AGGCCT 1 cut(s) 831
SsiI CCGC 2 cut(s) 592, 725
SspMI CTAG 2 cut(s) 278, 740
StuI AGGCCT 1 cut(s) 831
StyD4I CCNGG 3 cut(s) 86, 113, 338
TaaI ACNGT 4 cut(s) 388, 460, 814, 823
TaiI ACGT 3 cut(s) 225, 650, 761
TaqI TCGA 1 cut(s) 14
TasI AATT 5 cut(s) 152, 240, 345, 540, 687
TfiI GAWTC 2 cut(s) 16, 515
Tru1I TTAA 4 cut(s) 150, 171, 614, 644
Tru9I TTAA 4 cut(s) 150, 171, 614, 644
TscAI CASTG 2 cut(s) 289, 403
TseFI GTSAC 2 cut(s) 197, 332
TseI GCWGC 3 cut(s) 314, 452, 672
Tsp45I GTSAC 2 cut(s) 197, 332
TspDTI ATGAA 2 cut(s) 312, 628
TspGWI ACGGA 2 cut(s) 823, 841
TspRI CASTG 2 cut(s) 289, 403
VneI GTGCAC 1 cut(s) 360
XapI RAATTY 1 cut(s) 240
XspI CTAG 2 cut(s) 278, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.