pycom05g00070

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014489
Physical Location & Seq
Forward (+)
131688 .. 134052
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00070.1

Sequence Viewer

Length: 1269 bp
ATGGCTAATTCATCGAGTGGTGGTGTGCCTCCGGGATTCCGATTCCACCCCACGGACGAAGAGTTGCTTCACTACTATTTGAAGAAGAAGGTGTCCTTTCAGAAGTTTGACATGGAGGTCATTAGAGAGGTGGACTTGAATAAGATGGAACCTTGGGACTTGCAAGAGAGATGTAGGATTGGGTCGACTCCACAAAACGAGTGGTATTTTTTCAGCCACAAAGACAGGAAGTACCCAACAGGGTCGAGGACAAATAGGGCTACAAAGGCTGGGTTTTGGAAGGCAACAGGGCGGGACAAATGCATCAGAAACACCTTCAAGCAGATTGGCATGAGAAAAACCCTTGTTTTCTACAGAGGCAGAGCTCCCCACGGCCAAAAGACTGACTGGATTATGCACGAGTACCGCCTGGAAGATAGTGATGATCCTCATGGAAACCTATCTAGTGGTTTAGGATTTTTGTTACCTAATATATCTTTCTTTAATTCGGAGATTTTAATTGGTAACGATAAATATTGCAAACACATGCAGGAAGATGGGTGGGTGATCTGCAGGGTTTTCAAGAAGAAAAATTTATTCAAGGTTGCAAATGGAGGAAGCTCTAGCATGAACTCTTCAGACCATCAGCAGCTCAAAAATACATCAAGCAACAGCCAATTGCAAGCTCGCTCCTTCATGCATAGCCACGGAGAAAACCAGTACTTACTACGCCAACAACTCCATGCAGCAACCCAAGCATTCGAGCTAAACAACAACCCAAGCCTTCAGTACGCCCACCTGCAGCCACCCCCTCACTACTCTCCTTTCCAATCCCAAGCCCTTATCCCGACCTTCGCCCACAAGCCCCTTTCCGCCTGCGACGACTACTCATCCCAGCTCCCCTCCGATGATTCACCTAGCCACGGCATGGTCAAGCAGCTCATGACAAACCCTAGAGACTGTGAAAGTGGCAGTGAGGGCCTCCGGTACCAAGCGTGTGAGCCAGTGTTAGAGGTTGGCACGTGCGAACCAAACCATCAACTGAATACGGTTGCAGGAGGAGCTGGAGCAGCTGGCGGAAGAGATAATAATCATGATCACCAGGGGATGAGTGAATGGGCTATGCTCGACCGCCTGGTTACTTCTCATGACCTTGGAAATGATCAAGATTCGTCGTCCAAAGGAGCAGCCAGGTATGAGGATGCAAATGCACCCTCTTCTGTCAACCAAATTAACCCCCAGCACCTCTCGTTGCGTGGAGAGATGGATTTTTGGGGCTATGCAAAATAA

Protein Analysis

423

Amino Acids

47.69

Weight (kDa)

7.05

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 10 - 137 2.4e-46 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10350 AT4G10350
fragaria_vesca FvH4_2g00240
malus_domestica MD05G1005600.v1.1 MD10G1000900.v1.1
prunus_persica Prupe.8G002500_v2.0.a1
pyrus_communis pycom05g00070
rosa_chinensis RchiOBHm_Chr6g0249541
rosa_laevigata RLG00000015571
rosa_multiflora Rmu_ssc0000407.1_g000012
rosa_roxburghii Rroxscaffold_7G00217580
rosa_rugosa Rorug05G0493400
rosa_samantha Rh6AG003100 Rh6BG007000 Rh6CG002100 Rh6DG002100
rosa_wichuraiana Rw6G000360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 786
AasI GACNNNNNNGTC 1 cut(s) 116
Acc36I ACCTGC 1 cut(s) 786
Acc65I GGTACC 1 cut(s) 966
AccB1I GGYRCC 1 cut(s) 966
AccB7I CCANNNNNTGG 1 cut(s) 907
AccI GTMKAC 1 cut(s) 185
AciI CCGC 5 cut(s) 292, 406, 852, 1056, 1111
AclWI GGATC 1 cut(s) 419
AcoI YGGCCR 1 cut(s) 373
AcsI RAATTY 1 cut(s) 571
AcuI CTGAAG 2 cut(s) 600, 749
AcvI CACGTG 1 cut(s) 1002
AfaI GTAC 5 cut(s) 233, 404, 701, 770, 968
AfiI CCNNNNNNNGG 3 cut(s) 52, 902, 907
AgsI TTSAA 5 cut(s) 82, 139, 319, 562, 580
AjnI CCWGG 4 cut(s) 408, 1080, 1113, 1169
AluBI AGCT 9 cut(s) 365, 600, 631, 665, 745, 877, 919, 1043, 1052
AluI AGCT 9 cut(s) 365, 600, 631, 665, 745, 877, 919, 1043, 1052
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 1 cut(s) 930
AlwI GGATC 1 cut(s) 419
AoxI GGCC 2 cut(s) 373, 958
ApeKI GCWGC 6 cut(s) 628, 725, 781, 916, 1049, 1166
ApoI RAATTY 1 cut(s) 571
Asp718I GGTACC 1 cut(s) 966
AspS9I GGNCC 1 cut(s) 958
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 3 cut(s) 556, 885, 1070
BanI GGYRCC 1 cut(s) 966
BanII GRGCYC 1 cut(s) 367
BauI CACGAG 1 cut(s) 398
BbrPI CACGTG 1 cut(s) 1002
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 6 cut(s) 640, 737, 793, 928, 1061, 1178
BccI CCATC 5 cut(s) 139, 530, 630, 1023, 1237
BceAI ACGGC 2 cut(s) 388, 919
BciT130I CCWGG 4 cut(s) 410, 1082, 1115, 1171
BclI TGATCA 2 cut(s) 1075, 1141
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 930
BfaI CTAG 4 cut(s) 444, 603, 897, 933
BfmI CTRYAG 3 cut(s) 352, 550, 779
BfuAI ACCTGC 1 cut(s) 786
BisI GCNGC 6 cut(s) 629, 726, 782, 917, 1050, 1167
BlsI GCNGC 6 cut(s) 630, 727, 783, 918, 1051, 1168
BmcAI AGTACT 1 cut(s) 701
Bme1390I CCNGG 5 cut(s) 33, 410, 1082, 1115, 1171
BmgT120I GGNCC 1 cut(s) 958
BmiI GGNNCC 2 cut(s) 150, 968
BmrFI CCNGG 5 cut(s) 33, 410, 1082, 1115, 1171
BmsI GCATC 2 cut(s) 312, 1171
BpmI CTGGAG 1 cut(s) 1065
BpuMI CCSGG 1 cut(s) 33
BsaAI YACGTR 1 cut(s) 1002
BsaBI GATNNNNATC 1 cut(s) 1068
BsaJI CCNNGG 7 cut(s) 51, 152, 370, 685, 901, 1081, 1132
BsaWI WCCGGW 1 cut(s) 963
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 902, 907
Bse1I ACTGG 3 cut(s) 392, 697, 983
Bse8I GATNNNNATC 1 cut(s) 1068
BseBI CCWGG 4 cut(s) 410, 1082, 1115, 1171
BseDI CCNNGG 7 cut(s) 51, 152, 370, 685, 901, 1081, 1132
BseGI GGATG 3 cut(s) 869, 1092, 1186
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 3 cut(s) 52, 902, 907
BseNI ACTGG 3 cut(s) 392, 697, 983
BseRI GAGGAG 1 cut(s) 1053
BseXI GCAGC 6 cut(s) 640, 737, 793, 928, 1061, 1178
BseYI CCCAGC 3 cut(s) 269, 873, 1218
Bsh1285I CGRYCG 1 cut(s) 1111
BshFI GGCC 2 cut(s) 375, 960
BshNI GGYRCC 1 cut(s) 966
BsiEI CGRYCG 1 cut(s) 1111
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 2 cut(s) 32, 964
BslFI GGGAC 2 cut(s) 170, 308
BslI CCNNNNNNNGG 3 cut(s) 52, 902, 907
BsmAI GTCTC 1 cut(s) 930
BsmFI GGGAC 2 cut(s) 170, 308
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 2 cut(s) 375, 960
Bsp1286I GDGCHC 1 cut(s) 367
Bsp143I GATC 4 cut(s) 424, 546, 1075, 1141
BspACI CCGC 5 cut(s) 292, 406, 852, 1056, 1111
BspANI GGCC 2 cut(s) 375, 960
BspHI TCATGA 3 cut(s) 921, 1072, 1126
BspLI GGNNCC 2 cut(s) 150, 968
BspMAI CTGCAG 2 cut(s) 554, 783
BspMI ACCTGC 1 cut(s) 786
BspPI GGATC 1 cut(s) 419
BspT107I GGYRCC 1 cut(s) 966
BsrI ACTGG 3 cut(s) 392, 697, 983
BssECI CCNNGG 7 cut(s) 51, 152, 370, 685, 901, 1081, 1132
BssMI GATC 4 cut(s) 424, 546, 1075, 1141
BssSI CACGAG 1 cut(s) 398
BssT1I CCWWGG 2 cut(s) 152, 1132
Bst2BI CACGAG 1 cut(s) 398
Bst2UI CCWGG 4 cut(s) 410, 1082, 1115, 1171
Bst4CI ACNGT 2 cut(s) 941, 1030
Bst6I CTCTTC 4 cut(s) 54, 619, 1054, 1201
BstBAI YACGTR 1 cut(s) 1002
BstC8I GCNNGC 4 cut(s) 663, 667, 856, 1054
BstDSI CCRYGG 4 cut(s) 51, 370, 685, 901
BstF5I GGATG 3 cut(s) 869, 1092, 1186
BstKTI GATC 4 cut(s) 427, 549, 1078, 1144
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 4 cut(s) 424, 546, 1075, 1141
BstMCI CGRYCG 1 cut(s) 1111
BstMWI GCNNNNNNNGC 5 cut(s) 266, 734, 957, 1040, 1049
BstNI CCWGG 4 cut(s) 410, 1082, 1115, 1171
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 5 cut(s) 31, 408, 1080, 1113, 1169
BstSFI CTRYAG 3 cut(s) 352, 550, 779
BstV1I GCAGC 6 cut(s) 640, 737, 793, 928, 1061, 1178
BsuRI GGCC 2 cut(s) 375, 960
BtgI CCRYGG 4 cut(s) 51, 370, 685, 901
BtsCI GGATG 3 cut(s) 869, 1092, 1186
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 2 cut(s) 958, 990
BveI ACCTGC 1 cut(s) 786
Cac8I GCNNGC 4 cut(s) 663, 667, 856, 1054
CciI TCATGA 3 cut(s) 921, 1072, 1126
Cfr13I GGNCC 1 cut(s) 958
Csp6I GTAC 5 cut(s) 232, 403, 700, 769, 967
CspCI CAANNNNNGTGG 2 cut(s) 182, 217
CviQI GTAC 5 cut(s) 232, 403, 700, 769, 967
DpnI GATC 4 cut(s) 426, 548, 1077, 1143
DpnII GATC 4 cut(s) 424, 546, 1075, 1141
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 373
Eam1104I CTCTTC 4 cut(s) 54, 619, 1054, 1201
EarI CTCTTC 4 cut(s) 54, 619, 1054, 1201
EciI GGCGGA 2 cut(s) 841, 1071
Ecl136II GAGCTC 1 cut(s) 365
Eco130I CCWWGG 2 cut(s) 152, 1132
Eco24I GRGCYC 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 365
Eco57I CTGAAG 2 cut(s) 600, 749
Eco72I CACGTG 1 cut(s) 1002
EcoICRI GAGCTC 1 cut(s) 365
EcoO109I RGGNCCY 1 cut(s) 958
EcoRII CCWGG 4 cut(s) 408, 1080, 1113, 1169
EcoT14I CCWWGG 2 cut(s) 152, 1132
EcoT22I ATGCAT 2 cut(s) 305, 681
EcoT38I GRGCYC 1 cut(s) 367
ErhI CCWWGG 2 cut(s) 152, 1132
FalI AAGNNNNNCTT 4 cut(s) 51, 83, 80, 112
FaqI GGGAC 2 cut(s) 170, 308
FauI CCCGC 1 cut(s) 285
FbaI TGATCA 2 cut(s) 1075, 1141
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 6 cut(s) 629, 726, 782, 917, 1050, 1167
FokI GGATG 3 cut(s) 856, 1099, 1193
FriOI GRGCYC 1 cut(s) 367
Fsp4HI GCNGC 6 cut(s) 629, 726, 782, 917, 1050, 1167
FspBI CTAG 4 cut(s) 444, 603, 897, 933
GluI GCNGC 6 cut(s) 629, 726, 782, 917, 1050, 1167
GsaI CCCAGC 3 cut(s) 273, 877, 1222
GsuI CTGGAG 1 cut(s) 1065
HaeIII GGCC 2 cut(s) 375, 960
HapII CCGG 2 cut(s) 32, 964
HincII GTYRAC 2 cut(s) 186, 1204
HindII GTYRAC 2 cut(s) 186, 1204
HinfI GANTC 5 cut(s) 36, 42, 187, 890, 1148
HpaII CCGG 2 cut(s) 32, 964
HphI GGTGA 3 cut(s) 556, 885, 1070
Hpy166II GTNNAC 3 cut(s) 133, 186, 1204
Hpy188I TCNGA 6 cut(s) 41, 102, 308, 490, 619, 886
Hpy188III TCNNGA 6 cut(s) 562, 826, 922, 1073, 1127, 1145
Hpy8I GTNNAC 3 cut(s) 133, 186, 1204
Hpy99I CGWCG 2 cut(s) 863, 1156
HpyAV CCTTC 6 cut(s) 82, 274, 325, 682, 773, 841
HpyCH4III ACNGT 2 cut(s) 941, 1030
HpyCH4IV ACGT 1 cut(s) 1001
HpyF10VI GCNNNNNNNGC 5 cut(s) 266, 734, 957, 1040, 1049
HpySE526I ACGT 1 cut(s) 1001
KpnI GGTACC 1 cut(s) 970
Ksp22I TGATCA 2 cut(s) 1075, 1141
Kzo9I GATC 4 cut(s) 424, 546, 1075, 1141
LmnI GCTCC 6 cut(s) 370, 674, 882, 1040, 1046, 1163
Lsp1109I GCAGC 6 cut(s) 640, 737, 793, 928, 1061, 1178
LweI GCATC 2 cut(s) 312, 1171
MaeI CTAG 4 cut(s) 444, 603, 897, 933
MaeII ACGT 1 cut(s) 1001
MaeIII GTNAC 3 cut(s) 462, 503, 1117
MalI GATC 4 cut(s) 426, 548, 1077, 1143
MboI GATC 4 cut(s) 424, 546, 1075, 1141
MboII GAAGA 9 cut(s) 71, 94, 97, 425, 545, 577, 606, 1071, 1188
MfeI CAATTG 1 cut(s) 656
MhlI GDGCHC 1 cut(s) 367
MluCI AATT 6 cut(s) 7, 484, 498, 571, 656, 1209
MlyI GAGTC 1 cut(s) 181
Mph1103I ATGCAT 2 cut(s) 305, 681
MseI TTAA 3 cut(s) 483, 497, 1212
MspA1I CMGCKG 1 cut(s) 1052
MspI CCGG 2 cut(s) 32, 964
MspR9I CCNGG 5 cut(s) 33, 410, 1082, 1115, 1171
MunI CAATTG 1 cut(s) 656
Mva1269I GAATGC 1 cut(s) 737
MvaI CCWGG 4 cut(s) 410, 1082, 1115, 1171
MwoI GCNNNNNNNGC 5 cut(s) 266, 734, 957, 1040, 1049
NciI CCSGG 1 cut(s) 33
NdeII GATC 4 cut(s) 424, 546, 1075, 1141
NlaIV GGNNCC 2 cut(s) 150, 968
NsiI ATGCAT 2 cut(s) 305, 681
NspI RCATGY 1 cut(s) 529
PagI TCATGA 3 cut(s) 921, 1072, 1126
PaqCI CACCTGC 1 cut(s) 786
PctI GAATGC 1 cut(s) 737
PfeI GAWTC 4 cut(s) 36, 42, 890, 1148
PflMI CCANNNNNTGG 1 cut(s) 907
PfoI TCCNGGA 1 cut(s) 31
PkrI GCNGC 6 cut(s) 630, 727, 783, 918, 1051, 1168
PleI GAGTC 1 cut(s) 181
PmaCI CACGTG 1 cut(s) 1002
PmlI CACGTG 1 cut(s) 1002
PpsI GAGTC 1 cut(s) 181
Ppu21I YACGTR 1 cut(s) 1002
Psp124BI GAGCTC 1 cut(s) 367
Psp6I CCWGG 4 cut(s) 408, 1080, 1113, 1169
PspCI CACGTG 1 cut(s) 1002
PspFI CCCAGC 3 cut(s) 269, 873, 1218
PspGI CCWGG 4 cut(s) 408, 1080, 1113, 1169
PspN4I GGNNCC 2 cut(s) 150, 968
PspPI GGNCC 1 cut(s) 958
PstI CTGCAG 2 cut(s) 554, 783
PvuII CAGCTG 1 cut(s) 1052
RsaI GTAC 5 cut(s) 233, 404, 701, 770, 968
RsaNI GTAC 5 cut(s) 232, 403, 700, 769, 967
SacI GAGCTC 1 cut(s) 367
SalI GTCGAC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 483, 497, 1212
SatI GCNGC 6 cut(s) 629, 726, 782, 917, 1050, 1167
Sau3AI GATC 4 cut(s) 424, 546, 1075, 1141
Sau96I GGNCC 1 cut(s) 958
ScaI AGTACT 1 cut(s) 701
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 5 cut(s) 33, 410, 1082, 1115, 1171
SduI GDGCHC 1 cut(s) 367
SfaNI GCATC 2 cut(s) 312, 1171
SfcI CTRYAG 3 cut(s) 352, 550, 779
Sse9I AATT 6 cut(s) 7, 484, 498, 571, 656, 1209
SsiI CCGC 5 cut(s) 292, 406, 852, 1056, 1111
SspI AATATT 1 cut(s) 515
SspMI CTAG 4 cut(s) 444, 603, 897, 933
SstI GAGCTC 1 cut(s) 367
StyD4I CCNGG 5 cut(s) 31, 408, 1080, 1113, 1169
StyI CCWWGG 2 cut(s) 152, 1132
TaaI ACNGT 2 cut(s) 941, 1030
TaiI ACGT 1 cut(s) 1004
TaqI TCGA 5 cut(s) 14, 185, 245, 741, 1107
TasI AATT 6 cut(s) 7, 484, 498, 571, 656, 1209
TatI WGTACW 1 cut(s) 699
TfiI GAWTC 4 cut(s) 36, 42, 890, 1148
Tru1I TTAA 3 cut(s) 483, 497, 1212
Tru9I TTAA 3 cut(s) 483, 497, 1212
TscAI CASTG 2 cut(s) 958, 990
TseI GCWGC 6 cut(s) 628, 725, 781, 916, 1049, 1166
TspDTI ATGAA 2 cut(s) 623, 664
TspGWI ACGGA 2 cut(s) 68, 702
TspRI CASTG 2 cut(s) 958, 990
Van91I CCANNNNNTGG 1 cut(s) 907
XapI RAATTY 1 cut(s) 571
XceI RCATGY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 198
XmiI GTMKAC 1 cut(s) 185
XspI CTAG 4 cut(s) 444, 603, 897, 933
ZrmI AGTACT 1 cut(s) 701
Zsp2I ATGCAT 2 cut(s) 305, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.