Rroxscaffold_7G00217580

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014489
Physical Location & Seq
Reverse (-)
68051716 .. 68053963
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217580.1

Sequence Viewer

Length: 1230 bp
ATGGCAACATCGAGTGGTGGTGTTCCCCCAGGGTTCCGATTCCACCCTACCGACGAAGAGCTGCTTCACTATTATTTGAAGAAGAAGGTGTCCTTTCAGAAGTTTGACATGGAGGTCATCAGAGAGGTCGACTTGAACAAGATGGAACCTTGGGAGTTGCAAGAGAGATGTAGAATTGGGTCGACTCCGCAAAATGAGTGGTACTTTTTCAGCCACAAAGACAGGAAGTACCCGACAGGGTCAAGGACGAATAGGGCAACAAATGCTGGGTTTTGGAAGGCAACAGGGAGAGACAAGTGCATCAGAAACACCTATAAGAAGATTGGAATGAGGAAAACCCTGGTTTTCTACAGAGGAAGAGCACCCCATGGCCAAAAGACTGATTGGATTATGCATGAGTACCGCCTTGAAGATGGCGATGATCCTCATGGAAATTTATCTAGTGAAGATGGATGGGTGATATGTAGGGTGTTTAAAAAGAAGAATTTATTCAAGGTAACTGGGAATAATGAAGGAGGAAGCTCTAGCATGAACTCATCAGATCATCACCATCATCAACAGCTGACTATGAACACCAACCAATTGCAAGCTCGAACCTTCATGCATACGCATAGCAGCAGATCAGATACCAACCAATACTTACTACGCCAACAAGCCTTTGAACTAAACAGACCTCATGATCATGATCAAGATCAGGATCAGTTGCCACCCGCCCTCCACTATGCCCACCTTAATTTGCAGCCGCCTCCTCAATACTCTCTTTTCCACTCTCAAGCCCTTAATGTACCAACTAACTCCCACAAAACCCTTGCTGCCTATAATGACTATTCTACCCTCCCTTCCGATTCGTCACCTGGCCATGGCATGGTCAAGCAGCTCATGACCAACCCCAGAGACTGCGAGAGCACTGGCAGCGAGAGCCTCCGGTACCAAGCTTGTGAACCGGGCTTGGAAGTTAACGACACCACGTGCGAAACAAATCAGCAAGCGATAGTTAATGCAGGAGAAAGATCATCAGATGATCAAGGCATGAGCGAATGGGCTATGCTGGACCGGCTTGTAACTTCTCATCTTGGAAATGAAGATTCAACGTCCAAAGGTGGAGCTTCTGCAGGTGCGAGGTATGATCAGGACGGGAATGGATCCTCCACTGTCAACCAGATTAACCAGCAGCAGCAACAGCAGCTCTCTTTGCGTGGAGAGATGAATTTTTGGGGCTATGCAAAATAG

Protein Analysis

409

Amino Acids

46.62

Weight (kDa)

6.7

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 136 2.2e-46 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10350 AT4G10350
fragaria_vesca FvH4_2g00240
malus_domestica MD05G1005600.v1.1 MD10G1000900.v1.1
prunus_persica Prupe.8G002500_v2.0.a1
pyrus_communis pycom05g00070
rosa_chinensis RchiOBHm_Chr6g0249541
rosa_laevigata RLG00000015571
rosa_multiflora Rmu_ssc0000407.1_g000012
rosa_roxburghii Rroxscaffold_7G00217580
rosa_rugosa Rorug05G0493400
rosa_samantha Rh6AG003100 Rh6BG007000 Rh6CG002100 Rh6DG002100
rosa_wichuraiana Rw6G000360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1103
AasI GACNNNNNNGTC 1 cut(s) 113
Acc36I ACCTGC 1 cut(s) 1103
Acc65I GGTACC 1 cut(s) 927
AccB1I GGYRCC 1 cut(s) 927
AccB7I CCANNNNNTGG 1 cut(s) 865
AccI GTMKAC 2 cut(s) 129, 182
AciI CCGC 4 cut(s) 188, 403, 711, 743
AclWI GGATC 4 cut(s) 416, 705, 1137, 1150
AcoI YGGCCR 2 cut(s) 370, 856
AcsI RAATTY 3 cut(s) 433, 484, 1207
AcvI CACGTG 1 cut(s) 969
AdeI CACNNNGTG 1 cut(s) 969
AfaI GTAC 5 cut(s) 203, 230, 401, 786, 929
AfiI CCNNNNNNNGG 2 cut(s) 860, 865
AgsI TTSAA 6 cut(s) 79, 136, 410, 493, 662, 1089
AjnI CCWGG 3 cut(s) 28, 339, 853
AluBI AGCT 8 cut(s) 61, 522, 562, 590, 877, 935, 1106, 1186
AluI AGCT 8 cut(s) 61, 522, 562, 590, 877, 935, 1106, 1186
Alw21I GWGCWC 2 cut(s) 364, 908
Alw26I GTCTC 2 cut(s) 285, 888
AlwI GGATC 4 cut(s) 416, 705, 1137, 1150
AlwNI CAGNNNCTG 1 cut(s) 897
AoxI GGCC 2 cut(s) 370, 856
ApeKI GCWGC 9 cut(s) 61, 615, 739, 812, 874, 912, 1171, 1174, 1183
ApoI RAATTY 3 cut(s) 433, 484, 1207
Asp700I GAANNNNTTC 1 cut(s) 488
Asp718I GGTACC 1 cut(s) 927
AspS9I GGNCC 1 cut(s) 1051
AsuC2I CCSGG 1 cut(s) 945
AsuHPI GGTGA 3 cut(s) 469, 539, 843
AvaII GGWCC 1 cut(s) 1051
BalI TGGCCA 2 cut(s) 372, 858
BamHI GGATCC 1 cut(s) 1142
BanI GGYRCC 1 cut(s) 927
BbrPI CACGTG 1 cut(s) 969
Bbv12I GWGCWC 2 cut(s) 364, 908
BbvI GCAGC 9 cut(s) 48, 627, 751, 799, 886, 924, 1183, 1186, 1195
BccI CCATC 5 cut(s) 136, 407, 443, 447, 558
BciT130I CCWGG 3 cut(s) 30, 341, 855
BclI TGATCA 4 cut(s) 679, 685, 1021, 1126
BcnI CCSGG 1 cut(s) 945
BcoDI GTCTC 2 cut(s) 285, 888
BfaI CTAG 2 cut(s) 441, 525
BfmI CTRYAG 2 cut(s) 349, 1110
BfuAI ACCTGC 1 cut(s) 1103
Bme1390I CCNGG 4 cut(s) 30, 341, 855, 945
Bme18I GGWCC 1 cut(s) 1051
BmgT120I GGNCC 1 cut(s) 1051
BmiI GGNNCC 4 cut(s) 35, 147, 929, 1144
BmrFI CCNGG 4 cut(s) 30, 341, 855, 945
BmrI ACTGGG 1 cut(s) 510
BmsI GCATC 1 cut(s) 309
BmuI ACTGGG 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 756
BpuMI CCSGG 1 cut(s) 945
BsaAI YACGTR 1 cut(s) 969
BsaBI GATNNNNATC 3 cut(s) 684, 690, 696
BsaJI CCNNGG 6 cut(s) 28, 29, 149, 339, 367, 859
BsaWI WCCGGW 1 cut(s) 924
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 2 cut(s) 860, 865
Bse118I RCCGGY 1 cut(s) 1053
Bse1I ACTGG 2 cut(s) 505, 913
Bse8I GATNNNNATC 3 cut(s) 684, 690, 696
BseBI CCWGG 3 cut(s) 30, 341, 855
BseDI CCNNGG 6 cut(s) 28, 29, 149, 339, 367, 859
BseGI GGATG 1 cut(s) 458
BseJI GATNNNNATC 3 cut(s) 684, 690, 696
BseLI CCNNNNNNNGG 2 cut(s) 860, 865
BseNI ACTGG 2 cut(s) 505, 913
BseRI GAGGAG 1 cut(s) 738
BseXI GCAGC 9 cut(s) 48, 627, 751, 799, 886, 924, 1183, 1186, 1195
BseYI CCCAGC 1 cut(s) 266
BshFI GGCC 2 cut(s) 372, 858
BshNI GGYRCC 1 cut(s) 927
BsiHKAI GWGCWC 2 cut(s) 364, 908
BsiSI CCGG 3 cut(s) 925, 944, 1054
BslI CCNNNNNNNGG 2 cut(s) 860, 865
BsmAI GTCTC 2 cut(s) 285, 888
BsnI GGCC 2 cut(s) 372, 858
Bsp1286I GDGCHC 2 cut(s) 364, 908
Bsp19I CCATGG 2 cut(s) 367, 859
BspACI CCGC 4 cut(s) 188, 403, 711, 743
BspANI GGCC 2 cut(s) 372, 858
BspHI TCATGA 3 cut(s) 676, 682, 879
BspLI GGNNCC 4 cut(s) 35, 147, 929, 1144
BspMAI CTGCAG 1 cut(s) 1114
BspMI ACCTGC 1 cut(s) 1103
BspPI GGATC 4 cut(s) 416, 705, 1137, 1150
BspQI GCTCTTC 2 cut(s) 51, 352
BspT107I GGYRCC 1 cut(s) 927
BsrFI RCCGGY 1 cut(s) 1053
BsrI ACTGG 2 cut(s) 505, 913
BssAI RCCGGY 1 cut(s) 1053
BssECI CCNNGG 6 cut(s) 28, 29, 149, 339, 367, 859
BssT1I CCWWGG 3 cut(s) 149, 367, 859
Bst2UI CCWGG 3 cut(s) 30, 341, 855
Bst4CI ACNGT 1 cut(s) 1153
Bst6I CTCTTC 2 cut(s) 51, 352
BstAPI GCANNNNNTGC 1 cut(s) 263
BstBAI YACGTR 1 cut(s) 969
BstC8I GCNNGC 2 cut(s) 588, 987
BstDSI CCRYGG 2 cut(s) 367, 859
BstF5I GGATG 1 cut(s) 458
BstMAI GTCTC 2 cut(s) 285, 888
BstMWI GCNNNNNNNGC 7 cut(s) 263, 912, 918, 1054, 1180, 1183, 1192
BstNI CCWGG 3 cut(s) 30, 341, 855
BstSCI CCNGG 4 cut(s) 28, 339, 853, 943
BstSFI CTRYAG 2 cut(s) 349, 1110
BstV1I GCAGC 9 cut(s) 48, 627, 751, 799, 886, 924, 1183, 1186, 1195
BstX2I RGATCY 1 cut(s) 1142
BstYI RGATCY 1 cut(s) 1142
BsuRI GGCC 2 cut(s) 372, 858
BtgI CCRYGG 2 cut(s) 367, 859
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 1 cut(s) 458
BtsIMutI CAGTG 2 cut(s) 906, 1149
BveI ACCTGC 1 cut(s) 1103
Cac8I GCNNGC 2 cut(s) 588, 987
CaiI CAGNNNCTG 1 cut(s) 897
CciI TCATGA 3 cut(s) 676, 682, 879
Cfr10I RCCGGY 1 cut(s) 1053
Cfr13I GGNCC 1 cut(s) 1051
Csp6I GTAC 5 cut(s) 202, 229, 400, 785, 928
CspCI CAANNNNNGTGG 2 cut(s) 179, 214
CviQI GTAC 5 cut(s) 202, 229, 400, 785, 928
DraI TTTAAA 1 cut(s) 475
DraIII CACNNNGTG 1 cut(s) 969
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 2 cut(s) 370, 856
Eam1104I CTCTTC 2 cut(s) 51, 352
EarI CTCTTC 2 cut(s) 51, 352
Eco130I CCWWGG 3 cut(s) 149, 367, 859
Eco47I GGWCC 1 cut(s) 1051
Eco72I CACGTG 1 cut(s) 969
EcoRII CCWGG 3 cut(s) 28, 339, 853
EcoT14I CCWWGG 3 cut(s) 149, 367, 859
EcoT22I ATGCAT 2 cut(s) 396, 606
ErhI CCWWGG 3 cut(s) 149, 367, 859
FalI AAGNNNNNCTT 4 cut(s) 48, 80, 77, 109
FauI CCCGC 1 cut(s) 718
FbaI TGATCA 4 cut(s) 679, 685, 1021, 1126
FblI GTMKAC 2 cut(s) 129, 182
FokI GGATG 1 cut(s) 465
FspBI CTAG 2 cut(s) 441, 525
GsaI CCCAGC 1 cut(s) 270
HaeIII GGCC 2 cut(s) 372, 858
HapII CCGG 3 cut(s) 925, 944, 1054
HincII GTYRAC 4 cut(s) 130, 183, 958, 1156
HindII GTYRAC 4 cut(s) 130, 183, 958, 1156
HindIII AAGCTT 1 cut(s) 933
HinfI GANTC 4 cut(s) 39, 184, 845, 1085
HpaI GTTAAC 1 cut(s) 958
HpaII CCGG 3 cut(s) 925, 944, 1054
HphI GGTGA 3 cut(s) 469, 539, 843
Hpy166II GTNNAC 5 cut(s) 130, 183, 941, 958, 1156
Hpy188I TCNGA 8 cut(s) 38, 99, 122, 305, 541, 625, 844, 1018
Hpy188III TCNNGA 6 cut(s) 677, 683, 689, 695, 880, 1130
Hpy8I GTNNAC 5 cut(s) 130, 183, 941, 958, 1156
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 5 cut(s) 79, 271, 506, 607, 849
HpyCH4III ACNGT 1 cut(s) 1153
HpyCH4IV ACGT 2 cut(s) 968, 1091
HpyCH4V TGCA 9 cut(s) 160, 300, 394, 586, 604, 739, 1001, 1112, 1223
HpyF10VI GCNNNNNNNGC 7 cut(s) 263, 912, 918, 1054, 1180, 1183, 1192
HpySE526I ACGT 2 cut(s) 968, 1091
KpnI GGTACC 1 cut(s) 931
Ksp22I TGATCA 4 cut(s) 679, 685, 1021, 1126
KspAI GTTAAC 1 cut(s) 958
LguI GCTCTTC 2 cut(s) 51, 352
LmnI GCTCC 1 cut(s) 1103
Lsp1109I GCAGC 9 cut(s) 48, 627, 751, 799, 886, 924, 1183, 1186, 1195
LweI GCATC 1 cut(s) 309
MaeI CTAG 2 cut(s) 441, 525
MaeII ACGT 2 cut(s) 968, 1091
MaeIII GTNAC 3 cut(s) 496, 849, 1060
MboII GAAGA 9 cut(s) 68, 91, 94, 331, 369, 422, 458, 493, 1094
MfeI CAATTG 1 cut(s) 581
MflI RGATCY 1 cut(s) 1142
MhlI GDGCHC 2 cut(s) 364, 908
MlsI TGGCCA 2 cut(s) 372, 858
MluCI AATT 6 cut(s) 174, 433, 484, 581, 733, 1207
MluNI TGGCCA 2 cut(s) 372, 858
MlyI GAGTC 1 cut(s) 178
Mox20I TGGCCA 2 cut(s) 372, 858
Mph1103I ATGCAT 2 cut(s) 396, 606
MroXI GAANNNNTTC 1 cut(s) 488
MscI TGGCCA 2 cut(s) 372, 858
MseI TTAA 6 cut(s) 474, 732, 780, 957, 996, 1164
MslI CAYNNNNRTG 1 cut(s) 681
Msp20I TGGCCA 2 cut(s) 372, 858
MspA1I CMGCKG 1 cut(s) 562
MspI CCGG 3 cut(s) 925, 944, 1054
MspR9I CCNGG 4 cut(s) 30, 341, 855, 945
MunI CAATTG 1 cut(s) 581
MvaI CCWGG 3 cut(s) 30, 341, 855
MwoI GCNNNNNNNGC 7 cut(s) 263, 912, 918, 1054, 1180, 1183, 1192
NciI CCSGG 1 cut(s) 945
NcoI CCATGG 2 cut(s) 367, 859
NlaIV GGNNCC 4 cut(s) 35, 147, 929, 1144
NmuCI GTSAC 1 cut(s) 849
NsiI ATGCAT 2 cut(s) 396, 606
PagI TCATGA 3 cut(s) 676, 682, 879
PaqCI CACCTGC 1 cut(s) 1103
PasI CCCWGGG 1 cut(s) 29
PciSI GCTCTTC 2 cut(s) 51, 352
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 3 cut(s) 39, 845, 1085
PflFI GACNNNGTC 1 cut(s) 238
PflMI CCANNNNNTGG 1 cut(s) 865
PleI GAGTC 1 cut(s) 178
PmaCI CACGTG 1 cut(s) 969
PmlI CACGTG 1 cut(s) 969
PpsI GAGTC 1 cut(s) 178
Ppu21I YACGTR 1 cut(s) 969
Psp6I CCWGG 3 cut(s) 28, 339, 853
PspCI CACGTG 1 cut(s) 969
PspFI CCCAGC 1 cut(s) 266
PspGI CCWGG 3 cut(s) 28, 339, 853
PspN4I GGNNCC 4 cut(s) 35, 147, 929, 1144
PspPI GGNCC 1 cut(s) 1051
PstI CTGCAG 1 cut(s) 1114
PstNI CAGNNNCTG 1 cut(s) 897
PsuI RGATCY 1 cut(s) 1142
PsyI GACNNNGTC 1 cut(s) 238
PvuII CAGCTG 1 cut(s) 562
RsaI GTAC 5 cut(s) 203, 230, 401, 786, 929
RsaNI GTAC 5 cut(s) 202, 229, 400, 785, 928
RseI CAYNNNNRTG 1 cut(s) 681
SalI GTCGAC 2 cut(s) 128, 181
SapI GCTCTTC 2 cut(s) 51, 352
SaqAI TTAA 6 cut(s) 474, 732, 780, 957, 996, 1164
Sau96I GGNCC 1 cut(s) 1051
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 4 cut(s) 30, 341, 855, 945
SduI GDGCHC 2 cut(s) 364, 908
SfaNI GCATC 1 cut(s) 309
SfcI CTRYAG 2 cut(s) 349, 1110
SinI GGWCC 1 cut(s) 1051
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 1 cut(s) 771
SmoI CTYRAG 1 cut(s) 771
Sse9I AATT 6 cut(s) 174, 433, 484, 581, 733, 1207
SsiI CCGC 4 cut(s) 188, 403, 711, 743
SspMI CTAG 2 cut(s) 441, 525
StyD4I CCNGG 4 cut(s) 28, 339, 853, 943
StyI CCWWGG 3 cut(s) 149, 367, 859
TaaI ACNGT 1 cut(s) 1153
TaiI ACGT 2 cut(s) 971, 1094
TaqI TCGA 4 cut(s) 11, 129, 182, 592
TasI AATT 6 cut(s) 174, 433, 484, 581, 733, 1207
TauI GCSGC 1 cut(s) 745
TfiI GAWTC 3 cut(s) 39, 845, 1085
Tru1I TTAA 6 cut(s) 474, 732, 780, 957, 996, 1164
Tru9I TTAA 6 cut(s) 474, 732, 780, 957, 996, 1164
TscAI CASTG 2 cut(s) 913, 1156
TseFI GTSAC 1 cut(s) 849
TseI GCWGC 9 cut(s) 61, 615, 739, 812, 874, 912, 1171, 1174, 1183
Tsp45I GTSAC 1 cut(s) 849
TspDTI ATGAA 6 cut(s) 525, 545, 584, 589, 1095, 1220
TspRI CASTG 2 cut(s) 913, 1156
Tth111I GACNNNGTC 1 cut(s) 238
Van91I CCANNNNNTGG 1 cut(s) 865
VpaK11BI GGWCC 1 cut(s) 1051
XapI RAATTY 3 cut(s) 433, 484, 1207
XmiI GTMKAC 2 cut(s) 129, 182
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 2 cut(s) 441, 525
Zsp2I ATGCAT 2 cut(s) 396, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.