pycom05g00270

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005729
Physical Location & Seq
Forward (+)
333877 .. 335312
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00270.1

Sequence Viewer

Length: 1230 bp
ATGGGAAGAGAATTTGATGTTATTAGTACTCCAAAAATTTTATTTGACATTCCAAATTTTCTATCATTAAAAAACAATACACTTGTAATAAGTAAGCTCTCTTACTTTACACTCTGGGGCACGTGTATAAATCACATAACTCAGAAAAGTGCAAAGTTTGGTCAAATCCGGCAGAATCAAAGTCAAACTTGTCTCATGTCTTTATGTCCTATAAAAATCTCTTTGCCTTTACTTCACTCCCCAAACTTTCCATTTTTGCCATATTTGGCTGTCTCAGAAACGATAATATTGAGCATGAAGAACCTCCCCTTCCTCTTCCTTGCTATCCTCACCTTCCGTTGCATGAACCCCGTTTCCGCAGCCTGCCACGTCGACGACGAAGCGGGTCTGCTTGCCTTCAAATCGGCCATCACAGCCGACCCATCTAACAAGCTAAGCTCATGGATAAAGGGTACAGACTGCTGCACATGGGCGGGTACAAACTGCCTCAACAACCGGGTCACCAGCCTCTCCCTCTCGGGTCAACCCGACAAGCCCACTACTTACTTATCCGGTAAAATCTCGCCCTCCCTCTCCAAACTCAAATTCTTGAACGGCGTCTATCTCGAAAACCTTAACCTGTCGGGTCCCTTCCCGGATCTCCTATTCGGACTACCCGACCTCAAATACGTCTACATTGAACACAACAAGCTCTCGGGTCGAATACCCAACACTATCGGCAACTTAACCCAACTCGAAGCACTCAGCTTTTACTACAACCGGTTCACCGGTCCAATTCCGAGTTCAATCTCCAAGCTGACCCGGCTCACCCAGCTCAACCTTGGCGGCAACATCTTAACCGGTTCGATCCCGTCCGGCATTCAAAACCTCAAGAACCTCACCCTCTTCAACCTCCAGCAAAACGGATTGTCGGGTCCCATCCCCGATTTCTTCACATCGTTTCCGGAGCTCCGAGTCCTCAGTCTCTCCGGCAACTACTTTACCGGGAAAATCCCAGCTTCAATTTCCGCTTTGGCGCCAAAACTGGCCTACCTGGAGCTCGGCCACAACGCCCTGACCGGCCAAATCCCTGATTTTCTCGGCAATTTCAAGGCCCTCGACACCCTCAACCTCTCTAGCAACAAATTATCCGGTGTCGTACCTGAGAAGTTCGCGAATCTGACCAAGATCTTCAACCTGCAAGGATCGATCTTTCTCGTAATTACCTCACCGACCCATTTCCGGAACTGA

Protein Analysis

410

Amino Acids

44.88

Weight (kDa)

9.3

Isoelectric Point (pI)

30.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 125 - 162 3.1e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 186 - 295 7.4e-09 Leucine-rich repeat region
LRR_8 PF13855 220 - 277 2.3e-06 Leucine rich repeat
LRR_8 PF13855 268 - 326 3.1e-06 Leucine rich repeat
LRR_8 PF13855 292 - 352 8.8e-06 Leucine rich repeat
LRR_8 PF13855 340 - 393 1.6e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1185
AccB1I GGYRCC 1 cut(s) 1015
AccI GTMKAC 2 cut(s) 372, 672
AccII CGCG 1 cut(s) 1154
AccIII TCCGGA 2 cut(s) 943, 1221
AciI CCGC 5 cut(s) 357, 383, 473, 825, 1008
AclWI GGATC 3 cut(s) 645, 841, 1192
AcoI YGGCCR 3 cut(s) 405, 1042, 1060
AcsI RAATTY 4 cut(s) 11, 36, 55, 584
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 2 cut(s) 597, 1016
AfaI GTAC 4 cut(s) 28, 454, 478, 1140
AfiI CCNNNNNNNGG 1 cut(s) 1221
AflIII ACRYGT 1 cut(s) 122
AgeI ACCGGT 3 cut(s) 759, 767, 839
AgsI TTSAA 9 cut(s) 400, 592, 680, 786, 863, 889, 1002, 1090, 1174
AjiI CACGTC 1 cut(s) 370
AjnI CCWGG 1 cut(s) 1032
AjuI GAANNNNNNNTTGG 4 cut(s) 569, 601, 766, 798
AloI GAACNNNNNNTCC 2 cut(s) 338, 370
Alw21I GWGCWC 2 cut(s) 951, 1041
Alw26I GTCTC 3 cut(s) 197, 277, 968
AlwI GGATC 3 cut(s) 645, 841, 1192
Ama87I CYCGRG 2 cut(s) 517, 694
Aor13HI TCCGGA 2 cut(s) 943, 1221
AoxI GGCC 5 cut(s) 405, 1026, 1042, 1060, 1092
ApeKI GCWGC 2 cut(s) 359, 462
ApoI RAATTY 4 cut(s) 11, 36, 55, 584
AsiGI ACCGGT 3 cut(s) 759, 767, 839
AspLEI GCGC 1 cut(s) 1018
AspS9I GGNCC 4 cut(s) 626, 770, 914, 1093
AsuC2I CCSGG 4 cut(s) 497, 635, 802, 985
AsuHPI GGTGA 6 cut(s) 322, 493, 757, 799, 871, 1200
AvaI CYCGRG 2 cut(s) 517, 694
AvaII GGWCC 3 cut(s) 626, 770, 914
BaeGI GKGCMC 1 cut(s) 122
BanI GGYRCC 1 cut(s) 1015
BanII GRGCYC 2 cut(s) 951, 1041
BbrPI CACGTG 1 cut(s) 123
Bbv12I GWGCWC 2 cut(s) 951, 1041
BbvI GCAGC 2 cut(s) 371, 449
BccI CCATC 3 cut(s) 416, 430, 926
BceAI ACGGC 1 cut(s) 610
BciT130I CCWGG 1 cut(s) 1034
BcnI CCSGG 4 cut(s) 497, 635, 802, 985
BcoDI GTCTC 3 cut(s) 197, 277, 968
BfaI CTAG 1 cut(s) 1116
BfoI RGCGCY 1 cut(s) 1019
BfuAI ACCTGC 1 cut(s) 1185
BglII AGATCT 1 cut(s) 1167
BisI GCNGC 3 cut(s) 360, 463, 826
BlpI GCTNAGC 1 cut(s) 434
BlsI GCNGC 3 cut(s) 361, 464, 827
BmcAI AGTACT 1 cut(s) 28
Bme1390I CCNGG 5 cut(s) 497, 635, 802, 985, 1034
Bme18I GGWCC 3 cut(s) 626, 770, 914
BmeT110I CYCGRG 2 cut(s) 517, 694
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 4 cut(s) 626, 770, 914, 1093
BmiI GGNNCC 5 cut(s) 627, 628, 915, 916, 1017
BmrFI CCNGG 5 cut(s) 497, 635, 802, 985, 1034
BpmI CTGGAG 2 cut(s) 878, 1055
Bpu1102I GCTNAGC 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 854
BpuMI CCSGG 4 cut(s) 497, 635, 802, 985
Bsa29I ATCGAT 1 cut(s) 1187
BsaAI YACGTR 1 cut(s) 123
BsaHI GRCGYC 2 cut(s) 597, 1016
BsaJI CCNNGG 1 cut(s) 820
BsaWI WCCGGW 7 cut(s) 551, 759, 767, 839, 943, 1130, 1221
Bsc4I CCNNNNNNNGG 1 cut(s) 1221
Bse118I RCCGGY 4 cut(s) 759, 767, 839, 1058
Bse1I ACTGG 1 cut(s) 1029
BseAI TCCGGA 2 cut(s) 943, 1221
BseBI CCWGG 1 cut(s) 1034
BseCI ATCGAT 1 cut(s) 1187
BseDI CCNNGG 1 cut(s) 820
BseGI GGATG 1 cut(s) 918
BseLI CCNNNNNNNGG 1 cut(s) 1221
BseMII CTCAG 5 cut(s) 155, 288, 757, 973, 1134
BseNI ACTGG 1 cut(s) 1029
BseSI GKGCMC 1 cut(s) 122
BseXI GCAGC 2 cut(s) 371, 449
BseYI CCCAGC 2 cut(s) 810, 994
BsgI GTGCAG 1 cut(s) 448
Bsh1236I CGCG 1 cut(s) 1154
BshFI GGCC 5 cut(s) 407, 1028, 1044, 1062, 1094
BshNI GGYRCC 1 cut(s) 1015
BshTI ACCGGT 3 cut(s) 759, 767, 839
BshVI ATCGAT 1 cut(s) 1187
BsiHKAI GWGCWC 2 cut(s) 951, 1041
BsiHKCI CYCGRG 2 cut(s) 517, 694
BslFI GGGAC 2 cut(s) 612, 900
BslI CCNNNNNNNGG 1 cut(s) 1221
BsmAI GTCTC 3 cut(s) 197, 277, 968
BsmFI GGGAC 2 cut(s) 612, 900
BsmI GAATGC 1 cut(s) 858
BsnI GGCC 5 cut(s) 407, 1028, 1044, 1062, 1094
BsoBI CYCGRG 2 cut(s) 517, 694
Bsp1286I GDGCHC 3 cut(s) 122, 951, 1041
Bsp13I TCCGGA 2 cut(s) 943, 1221
Bsp143I GATC 5 cut(s) 637, 846, 1167, 1184, 1188
Bsp1720I GCTNAGC 1 cut(s) 434
Bsp68I TCGCGA 1 cut(s) 1154
BspACI CCGC 5 cut(s) 357, 383, 473, 825, 1008
BspANI GGCC 5 cut(s) 407, 1028, 1044, 1062, 1094
BspCNI CTCAG 5 cut(s) 154, 287, 756, 972, 1135
BspDI ATCGAT 1 cut(s) 1187
BspEI TCCGGA 2 cut(s) 943, 1221
BspFNI CGCG 1 cut(s) 1154
BspLI GGNNCC 5 cut(s) 627, 628, 915, 916, 1017
BspMI ACCTGC 1 cut(s) 1185
BspPI GGATC 3 cut(s) 645, 841, 1192
BspT107I GGYRCC 1 cut(s) 1015
BsrFI RCCGGY 4 cut(s) 759, 767, 839, 1058
BsrI ACTGG 1 cut(s) 1029
BssAI RCCGGY 4 cut(s) 759, 767, 839, 1058
BssECI CCNNGG 1 cut(s) 820
BssMI GATC 5 cut(s) 637, 846, 1167, 1184, 1188
BssNI GRCGYC 2 cut(s) 597, 1016
BssT1I CCWWGG 1 cut(s) 820
Bst2UI CCWGG 1 cut(s) 1034
Bst6I CTCTTC 2 cut(s) 320, 890
BstACI GRCGYC 2 cut(s) 597, 1016
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 364, 393
BstDEI CTNAG 6 cut(s) 141, 274, 434, 743, 959, 1143
BstEII GGTNACC 1 cut(s) 499
BstF5I GGATG 1 cut(s) 918
BstFNI CGCG 1 cut(s) 1154
BstH2I RGCGCY 1 cut(s) 1019
BstHHI GCGC 1 cut(s) 1018
BstKTI GATC 5 cut(s) 640, 849, 1170, 1187, 1191
BstMAI GTCTC 3 cut(s) 197, 277, 968
BstMBI GATC 5 cut(s) 637, 846, 1167, 1184, 1188
BstMWI GCNNNNNNNGC 3 cut(s) 413, 802, 811
BstNI CCWGG 1 cut(s) 1034
BstPI GGTNACC 1 cut(s) 499
BstSCI CCNGG 5 cut(s) 495, 633, 800, 983, 1032
BstSLI GKGCMC 1 cut(s) 122
BstUI CGCG 1 cut(s) 1154
BstV1I GCAGC 2 cut(s) 371, 449
BstX2I RGATCY 2 cut(s) 637, 1167
BstYI RGATCY 2 cut(s) 637, 1167
Bsu15I ATCGAT 1 cut(s) 1187
BsuRI GGCC 5 cut(s) 407, 1028, 1044, 1062, 1094
BsuTUI ATCGAT 1 cut(s) 1187
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 1 cut(s) 918
BtuMI TCGCGA 1 cut(s) 1154
BveI ACCTGC 1 cut(s) 1185
Cac8I GCNNGC 2 cut(s) 364, 393
CfoI GCGC 1 cut(s) 1018
Cfr10I RCCGGY 4 cut(s) 759, 767, 839, 1058
Cfr13I GGNCC 4 cut(s) 626, 770, 914, 1093
ClaI ATCGAT 1 cut(s) 1187
CseI GACGC 1 cut(s) 586
Csp6I GTAC 4 cut(s) 27, 453, 477, 1139
CspAI ACCGGT 3 cut(s) 759, 767, 839
CviAII CATG 5 cut(s) 196, 295, 343, 441, 468
CviQI GTAC 4 cut(s) 27, 453, 477, 1139
DdeI CTNAG 6 cut(s) 141, 274, 434, 743, 959, 1143
DinI GGCGCC 1 cut(s) 1017
DpnI GATC 5 cut(s) 639, 848, 1169, 1186, 1190
DpnII GATC 5 cut(s) 637, 846, 1167, 1184, 1188
EaeI YGGCCR 3 cut(s) 405, 1042, 1060
Eam1104I CTCTTC 2 cut(s) 320, 890
EarI CTCTTC 2 cut(s) 320, 890
Ecl136II GAGCTC 2 cut(s) 949, 1039
Eco130I CCWWGG 1 cut(s) 820
Eco24I GRGCYC 2 cut(s) 951, 1041
Eco47I GGWCC 3 cut(s) 626, 770, 914
Eco53kI GAGCTC 2 cut(s) 949, 1039
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 2 cut(s) 517, 694
Eco91I GGTNACC 1 cut(s) 499
EcoICRI GAGCTC 2 cut(s) 949, 1039
EcoO109I RGGNCCY 3 cut(s) 626, 914, 1093
EcoO65I GGTNACC 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 1032
EcoT14I CCWWGG 1 cut(s) 820
EcoT38I GRGCYC 2 cut(s) 951, 1041
EgeI GGCGCC 1 cut(s) 1017
EheI GGCGCC 1 cut(s) 1017
ErhI CCWWGG 1 cut(s) 820
FaeI CATG 5 cut(s) 199, 298, 346, 444, 471
FalI AAGNNNNNCTT 2 cut(s) 172, 204
FaqI GGGAC 2 cut(s) 612, 900
FatI CATG 5 cut(s) 195, 294, 342, 440, 467
FauI CCCGC 2 cut(s) 376, 466
FblI GTMKAC 2 cut(s) 372, 672
Fnu4HI GCNGC 3 cut(s) 360, 463, 826
FokI GGATG 1 cut(s) 905
FriOI GRGCYC 2 cut(s) 951, 1041
Fsp4HI GCNGC 3 cut(s) 360, 463, 826
FspBI CTAG 1 cut(s) 1116
GlaI GCGC 1 cut(s) 1017
GluI GCNGC 3 cut(s) 360, 463, 826
GsaI CCCAGC 2 cut(s) 814, 998
GsuI CTGGAG 2 cut(s) 878, 1055
HaeII RGCGCY 1 cut(s) 1019
HaeIII GGCC 5 cut(s) 407, 1028, 1044, 1062, 1094
HgaI GACGC 1 cut(s) 586
HhaI GCGC 1 cut(s) 1018
Hin1I GRCGYC 2 cut(s) 597, 1016
Hin1II CATG 5 cut(s) 199, 298, 346, 444, 471
Hin6I GCGC 1 cut(s) 1016
HinP1I GCGC 1 cut(s) 1016
HincII GTYRAC 2 cut(s) 373, 524
HindII GTYRAC 2 cut(s) 373, 524
HinfI GANTC 3 cut(s) 175, 954, 1156
HphI GGTGA 6 cut(s) 322, 493, 757, 799, 871, 1200
Hpy166II GTNNAC 4 cut(s) 373, 524, 673, 765
Hpy188I TCNGA 6 cut(s) 144, 277, 650, 780, 953, 1161
Hpy188III TCNNGA 6 cut(s) 589, 605, 871, 944, 1153, 1222
Hpy8I GTNNAC 4 cut(s) 373, 524, 673, 765
Hpy99I CGWCG 3 cut(s) 374, 377, 380
HpyAV CCTTC 4 cut(s) 319, 343, 406, 640
HpyCH4IV ACGT 3 cut(s) 122, 369, 669
HpyCH4V TGCA 4 cut(s) 152, 342, 465, 1180
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 802, 811
HpyF3I CTNAG 6 cut(s) 141, 274, 434, 743, 959, 1143
HpySE526I ACGT 3 cut(s) 122, 369, 669
Hsp92I GRCGYC 2 cut(s) 597, 1016
Hsp92II CATG 5 cut(s) 199, 298, 346, 444, 471
HspAI GCGC 1 cut(s) 1016
KasI GGCGCC 1 cut(s) 1015
KflI GGGWCCC 2 cut(s) 626, 914
Kpn2I TCCGGA 2 cut(s) 943, 1221
Kzo9I GATC 5 cut(s) 637, 846, 1167, 1184, 1188
LmnI GCTCC 3 cut(s) 946, 954, 1036
Lsp1109I GCAGC 2 cut(s) 371, 449
MaeI CTAG 1 cut(s) 1116
MaeII ACGT 3 cut(s) 122, 369, 669
MaeIII GTNAC 1 cut(s) 499
MalI GATC 5 cut(s) 639, 848, 1169, 1186, 1190
MboI GATC 5 cut(s) 637, 846, 1167, 1184, 1188
MboII GAAGA 6 cut(s) 18, 307, 310, 877, 922, 1162
MflI RGATCY 2 cut(s) 637, 1167
MhlI GDGCHC 3 cut(s) 122, 951, 1041
MluCI AATT 9 cut(s) 11, 36, 55, 584, 774, 1002, 1084, 1124, 1200
Mly113I GGCGCC 1 cut(s) 1016
MlyI GAGTC 1 cut(s) 963
MroI TCCGGA 2 cut(s) 943, 1221
MseI TTAA 4 cut(s) 68, 615, 725, 836
MspR9I CCNGG 5 cut(s) 497, 635, 802, 985, 1034
Mva1269I GAATGC 1 cut(s) 858
MvaI CCWGG 1 cut(s) 1034
MvnI CGCG 1 cut(s) 1154
MwoI GCNNNNNNNGC 3 cut(s) 413, 802, 811
NarI GGCGCC 1 cut(s) 1016
NciI CCSGG 4 cut(s) 497, 635, 802, 985
NdeII GATC 5 cut(s) 637, 846, 1167, 1184, 1188
NlaIII CATG 5 cut(s) 199, 298, 346, 444, 471
NlaIV GGNNCC 5 cut(s) 627, 628, 915, 916, 1017
NmeAIII GCCGAG 2 cut(s) 1020, 1059
NmuCI GTSAC 1 cut(s) 499
NruI TCGCGA 1 cut(s) 1154
PcsI WCGNNNNNNNCGW 2 cut(s) 375, 654
PctI GAATGC 1 cut(s) 858
PfeI GAWTC 2 cut(s) 175, 1156
PfoI TCCNGGA 1 cut(s) 633
PinAI ACCGGT 3 cut(s) 759, 767, 839
PkrI GCNGC 3 cut(s) 361, 464, 827
PleI GAGTC 1 cut(s) 962
PluTI GGCGCC 1 cut(s) 1019
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 1 cut(s) 962
Ppu21I YACGTR 1 cut(s) 123
PpuMI RGGWCCY 2 cut(s) 626, 914
Psp124BI GAGCTC 2 cut(s) 951, 1041
Psp5II RGGWCCY 2 cut(s) 626, 914
Psp6I CCWGG 1 cut(s) 1032
PspCI CACGTG 1 cut(s) 123
PspEI GGTNACC 1 cut(s) 499
PspFI CCCAGC 2 cut(s) 810, 994
PspGI CCWGG 1 cut(s) 1032
PspN4I GGNNCC 5 cut(s) 627, 628, 915, 916, 1017
PspPI GGNCC 4 cut(s) 626, 770, 914, 1093
PspPPI RGGWCCY 2 cut(s) 626, 914
PsuI RGATCY 2 cut(s) 637, 1167
RruI TCGCGA 1 cut(s) 1154
RsaI GTAC 4 cut(s) 28, 454, 478, 1140
RsaNI GTAC 4 cut(s) 27, 453, 477, 1139
SacI GAGCTC 2 cut(s) 951, 1041
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 4 cut(s) 68, 615, 725, 836
SatI GCNGC 3 cut(s) 360, 463, 826
Sau3AI GATC 5 cut(s) 637, 846, 1167, 1184, 1188
Sau96I GGNCC 4 cut(s) 626, 770, 914, 1093
ScaI AGTACT 1 cut(s) 28
SchI GAGTC 1 cut(s) 963
ScrFI CCNGG 5 cut(s) 497, 635, 802, 985, 1034
SduI GDGCHC 3 cut(s) 122, 951, 1041
SfoI GGCGCC 1 cut(s) 1017
SgrDI CGTCGACG 1 cut(s) 371
SinI GGWCC 3 cut(s) 626, 770, 914
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
Sse9I AATT 9 cut(s) 11, 36, 55, 584, 774, 1002, 1084, 1124, 1200
SsiI CCGC 5 cut(s) 357, 383, 473, 825, 1008
SspDI GGCGCC 1 cut(s) 1015
SspI AATATT 1 cut(s) 288
SspMI CTAG 1 cut(s) 1116
SstI GAGCTC 2 cut(s) 951, 1041
StyD4I CCNGG 5 cut(s) 495, 633, 800, 983, 1032
StyI CCWWGG 1 cut(s) 820
TaiI ACGT 3 cut(s) 125, 372, 672
TaqI TCGA 7 cut(s) 372, 606, 700, 735, 845, 1098, 1187
TasI AATT 9 cut(s) 11, 36, 55, 584, 774, 1002, 1084, 1124, 1200
TatI WGTACW 1 cut(s) 26
TauI GCSGC 1 cut(s) 828
TfiI GAWTC 2 cut(s) 175, 1156
Tru1I TTAA 4 cut(s) 68, 615, 725, 836
Tru9I TTAA 4 cut(s) 68, 615, 725, 836
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 2 cut(s) 359, 462
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 2 cut(s) 311, 359
TspGWI ACGGA 2 cut(s) 326, 918
VpaK11BI GGWCC 3 cut(s) 626, 770, 914
XapI RAATTY 4 cut(s) 11, 36, 55, 584
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmiI GTMKAC 2 cut(s) 372, 672
XspI CTAG 1 cut(s) 1116
ZrmI AGTACT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.