pycom05g06310

14-3-3-like protein 16R

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007005
Physical Location & Seq
Forward (+)
8535995 .. 8538184
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 672 bp
ATGGCAGCTTCATCCCCACGAGAAGAGAACGTGTACGTCGCCAAGCTCGCCGAGCAAGCCGAGCGATACGAGGAGATGGTTGAGTTCATGGAGAAGGTCGTCTCCTCCCTCCCCGAAGGCGAAGAGCCCACCGTCGAAGAGCGCAACCTCCTCTCCGTCGCCTACAAGAACGTGATCGGTGCCCGCCGTGCCTCGTGGCGCATCGTCTCCTCCATCGAGCAGAAGGAGGAGAGCCGCGGCAACAGCGACCACGTCGCCACTATCAAGGAGTACAGGGCTCGGATCGAGAACGAGCTCTCCAACATCTGCGGTGGGATTCTCAAGGTTCTCGAATCCAAGCTTGTTCCTGCGGCCAACGTTGGAGAGTCCAAGGTGTTTTATTTGAAGATGAAGGGAGATTACCATAGGTATTTGGCCGAGTTCAAAACTGGAGATGAGCGCAAGGAGGCGGCTGAGAACACCCTTAATTCCTATAAGTCAGCTCAGGTTTTTGCAACCTCTGAGTTGGCCCCAACTCATCCCATCCGTTTAGGATTGGCTTTGAACTTCTCTGTGTTTTACTACGAGATTCTAAATTCTCCTGACCGGGCTTGCAGTCTTGCCAAGCTTTTGATGAAGCCATTGCAGAGCTGGATACACTGGGAGAGGATTCATACAAGGATAGCACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

224

Amino Acids

25.37

Weight (kDa)

6.26

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 14 - 202 6.8e-81 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 1 cut(s) 237
AciI CCGC 6 cut(s) 184, 235, 237, 309, 350, 449
AclI AACGTT 1 cut(s) 357
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 2 cut(s) 351, 414
AcsI RAATTY 1 cut(s) 574
AfaI GTAC 2 cut(s) 35, 272
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 3 cut(s) 385, 424, 544
AjiI CACGTC 1 cut(s) 253
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
AluBI AGCT 7 cut(s) 8, 46, 295, 340, 482, 607, 630
AluI AGCT 7 cut(s) 8, 46, 295, 340, 482, 607, 630
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 106, 211
AlwI GGATC 1 cut(s) 290
AoxI GGCC 3 cut(s) 351, 414, 507
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 574
AspLEI GCGC 3 cut(s) 144, 201, 441
AspS9I GGNCC 1 cut(s) 508
AsuC2I CCSGG 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 184
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 3 cut(s) 129, 280, 297
BarI GAAGNNNNNNTAC 2 cut(s) 383, 415
BauI CACGAG 2 cut(s) 18, 193
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 70, 221, 530
BceAI ACGGC 1 cut(s) 171
BciVI GTATCC 1 cut(s) 627
BcnI CCSGG 1 cut(s) 587
BcoDI GTCTC 2 cut(s) 106, 211
BfuI GTATCC 1 cut(s) 627
BisI GCNGC 5 cut(s) 6, 235, 238, 351, 450
BlsI GCNGC 5 cut(s) 7, 236, 239, 352, 451
Bme1390I CCNGG 1 cut(s) 587
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 2 cut(s) 181, 510
BmrFI CCNGG 1 cut(s) 587
BmrI ACTGGG 1 cut(s) 649
BmsI GCATC 1 cut(s) 210
BmuI ACTGGG 1 cut(s) 649
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 587
BsaJI CCNNGG 2 cut(s) 235, 369
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 281, 311
Bse1I ACTGG 2 cut(s) 433, 644
Bse3DI GCAATG 1 cut(s) 620
BseDI CCNNGG 2 cut(s) 235, 369
BseGI GGATG 3 cut(s) 11, 517, 522
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 3 cut(s) 444, 492, 497
BseNI ACTGG 2 cut(s) 433, 644
BseRI GAGGAG 5 cut(s) 86, 94, 140, 199, 242
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 3 cut(s) 353, 416, 509
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 1 cut(s) 586
BsmAI GTCTC 2 cut(s) 106, 211
BsmBI CGTCTC 2 cut(s) 106, 211
BsnI GGCC 3 cut(s) 353, 416, 509
Bsp1286I GDGCHC 4 cut(s) 129, 184, 280, 297
Bsp143I GATC 2 cut(s) 174, 282
BspACI CCGC 6 cut(s) 184, 235, 237, 309, 350, 449
BspANI GGCC 3 cut(s) 353, 416, 509
BspCNI CTCAG 3 cut(s) 445, 493, 496
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 181, 510
BspPI GGATC 1 cut(s) 290
BspQI GCTCTTC 2 cut(s) 117, 132
BspT107I GGYRCC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 620
BsrI ACTGG 2 cut(s) 433, 644
BssECI CCNNGG 2 cut(s) 235, 369
BssMI GATC 2 cut(s) 174, 282
BssSI CACGAG 2 cut(s) 18, 193
BssT1I CCWWGG 1 cut(s) 369
Bst2BI CACGAG 2 cut(s) 18, 193
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 3 cut(s) 18, 117, 132
BstC8I GCNNGC 4 cut(s) 48, 57, 184, 592
BstDEI CTNAG 3 cut(s) 453, 483, 501
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 3 cut(s) 11, 517, 522
BstFNI CGCG 1 cut(s) 237
BstHHI GCGC 3 cut(s) 144, 201, 441
BstKTI GATC 2 cut(s) 177, 285
BstMAI GTCTC 2 cut(s) 106, 211
BstMBI GATC 2 cut(s) 174, 282
BstMWI GCNNNNNNNGC 6 cut(s) 47, 52, 56, 61, 188, 243
BstSCI CCNGG 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 184
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 17
BsuI GTATCC 1 cut(s) 627
BsuRI GGCC 3 cut(s) 353, 416, 509
BtgI CCRYGG 1 cut(s) 235
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 3 cut(s) 11, 517, 522
BtsIMutI CAGTG 1 cut(s) 637
Cac8I GCNNGC 4 cut(s) 48, 57, 184, 592
CfoI GCGC 3 cut(s) 144, 201, 441
Cfr13I GGNCC 1 cut(s) 508
Cfr42I CCGCGG 1 cut(s) 238
Csp6I GTAC 2 cut(s) 34, 271
CviAII CATG 1 cut(s) 88
CviQI GTAC 2 cut(s) 34, 271
DdeI CTNAG 3 cut(s) 453, 483, 501
DpnI GATC 2 cut(s) 176, 284
DpnII GATC 2 cut(s) 174, 282
EaeI YGGCCR 2 cut(s) 351, 414
Eam1104I CTCTTC 3 cut(s) 18, 117, 132
EarI CTCTTC 3 cut(s) 18, 117, 132
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 3 cut(s) 129, 280, 297
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 369
EcoT38I GRGCYC 3 cut(s) 129, 280, 297
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 2 cut(s) 106, 211
FaeI CATG 1 cut(s) 91
FaiI YATR 4 cut(s) 89, 405, 474, 654
FatI CATG 1 cut(s) 87
FauI CCCGC 1 cut(s) 191
Fnu4HI GCNGC 5 cut(s) 6, 235, 238, 351, 450
FokI GGATG 2 cut(s) 504, 509
FriOI GRGCYC 3 cut(s) 129, 280, 297
Fsp4HI GCNGC 5 cut(s) 6, 235, 238, 351, 450
GlaI GCGC 3 cut(s) 143, 200, 440
GluI GCNGC 5 cut(s) 6, 235, 238, 351, 450
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 3 cut(s) 353, 416, 509
HapII CCGG 1 cut(s) 586
HhaI GCGC 3 cut(s) 144, 201, 441
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 3 cut(s) 142, 199, 439
HinP1I GCGC 3 cut(s) 142, 199, 439
HindIII AAGCTT 2 cut(s) 338, 605
HinfI GANTC 5 cut(s) 316, 332, 365, 568, 649
HpaII CCGG 1 cut(s) 586
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 2 cut(s) 282, 502
Hpy188III TCNNGA 3 cut(s) 286, 329, 581
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 4 cut(s) 41, 137, 161, 257
HpyAV CCTTC 4 cut(s) 88, 110, 217, 385
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 5 cut(s) 30, 36, 171, 252, 357
HpyCH4V TGCA 3 cut(s) 494, 594, 625
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 52, 56, 61, 188, 243
HpyF3I CTNAG 3 cut(s) 453, 483, 501
HpySE526I ACGT 5 cut(s) 30, 36, 171, 252, 357
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 3 cut(s) 142, 199, 439
KspI CCGCGG 1 cut(s) 238
Kzo9I GATC 2 cut(s) 174, 282
LguI GCTCTTC 2 cut(s) 117, 132
LpnPI CCDG 8 cut(s) 259, 360, 414, 470, 594, 599, 616, 625
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 210
MaeII ACGT 5 cut(s) 30, 36, 171, 252, 357
MalI GATC 2 cut(s) 176, 284
MboI GATC 2 cut(s) 174, 282
MboII GAAGA 4 cut(s) 35, 134, 149, 397
MhlI GDGCHC 4 cut(s) 129, 184, 280, 297
MluCI AATT 2 cut(s) 466, 574
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 2 cut(s) 324, 340
MseI TTAA 1 cut(s) 465
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 1 cut(s) 586
MspR9I CCNGG 1 cut(s) 587
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 6 cut(s) 47, 52, 56, 61, 188, 243
NciI CCSGG 1 cut(s) 587
NdeII GATC 2 cut(s) 174, 282
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 2 cut(s) 181, 510
NmeAIII GCCGAG 3 cut(s) 76, 85, 442
PciSI GCTCTTC 2 cut(s) 117, 132
PfeI GAWTC 4 cut(s) 316, 332, 568, 649
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 5 cut(s) 7, 236, 239, 352, 451
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 297
Psp1406I AACGTT 1 cut(s) 357
PspN4I GGNNCC 2 cut(s) 181, 510
PspPI GGNCC 1 cut(s) 508
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 2 cut(s) 35, 272
RsaNI GTAC 2 cut(s) 34, 271
SacI GAGCTC 1 cut(s) 297
SacII CCGCGG 1 cut(s) 238
SapI GCTCTTC 2 cut(s) 117, 132
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 5 cut(s) 6, 235, 238, 351, 450
Sau3AI GATC 2 cut(s) 174, 282
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 1 cut(s) 587
SduI GDGCHC 4 cut(s) 129, 184, 280, 297
SfaNI GCATC 1 cut(s) 210
Sfr303I CCGCGG 1 cut(s) 238
SgrBI CCGCGG 1 cut(s) 238
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 2 cut(s) 466, 574
SsiI CCGC 6 cut(s) 184, 235, 237, 309, 350, 449
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 585
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 5 cut(s) 33, 39, 174, 255, 360
TaqI TCGA 4 cut(s) 135, 216, 285, 330
TasI AATT 2 cut(s) 466, 574
TatI WGTACW 1 cut(s) 270
TauI GCSGC 4 cut(s) 237, 240, 353, 452
TfiI GAWTC 4 cut(s) 316, 332, 568, 649
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 644
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 4 cut(s) 76, 404, 629, 641
TspGWI ACGGA 2 cut(s) 145, 515
TspRI CASTG 1 cut(s) 644
Tth111I GACNNNGTC 1 cut(s) 251
XapI RAATTY 1 cut(s) 574
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.