pycom05g08580

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013606
Physical Location & Seq
Forward (+)
11166702 .. 11167670
969 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g08580.1

Sequence Viewer

Length: 969 bp
ATGAAAACCACCAACAGCACCGCCACCACCACCGCCAACACAAACACAAAACTCATCCTCCTCCACCCCCAAGCAATCCACAAACCCACCACAAGCGGCGGAAGCCACCGCCTGTGGCTCCTCTTCTTCTTCACCTTCTTTACCCTCGCATTCACCCTCACTCTCATCAGCACCACCATCCCCCAAACCCTCCCCACCTCCTCCCTCACGCCCACCTCCGTACTCCCCCTCCCGCCCGCCGTCCTCGACGCACTTCTTCACTACGCCATTTCATCATCAAACACCTCGTCAGCAACCCACATGTCCCCGCCGGAACTTACCTCCATATCCTCCGCCCTCACCCGCCTCTGCGCACCTAACTGCAATTTCCTCGTTTTCGGCCTCACCCACGAGTCCCTCTTCTACCACACGCTCAACCTCAACGGCCGCACGGCTTTCATCGATGAGAGCGAGTTCCTCGTCTCCCGATTCGAGCAGGCCCACTCCCACCACAGCCTCGAGGCGTACGACGTGTCGTACGTGACCCAAGTCAAGGACCACAAACAATTGCTGTCAACGACCAAATCCCAGGTCAAAAACGAGTGCCGGCCGGTCCAGAACTTGTTGTTCTCGGACTGCAAGATCGGGATCAACGACTTGCCGAACCACATATATCAAGTCCCTTGGGATGTGATTCTGGTCGACGGCCCACGTGGGTATTCTCCGTCATCTCCGGGGCGGATGTCGGCTATTTTCACCGCCGGAGTTTTGGCCCGGAGCAAGAGGGGCGGTAGGGATAAAACTCATGTTTTTGTTCATGATTTTTCGAGGGAGGTTGAGAGGATTTTTAGCGACGAGTTTCTTTGCCGGGAGAACTTGGTGGAGGCTGTGGGTAGTTTGGGGCATTTTGTGGTGGAGAAAATGGAGGATCATAGCTTTGAGTTTTGCAGAAATTCACCATCATCTTCAAAAGAGGACGAAGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

323

Amino Acids

35.84

Weight (kDa)

6.23

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 105 - 296 1.3e-74 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013606)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05560
malus_domestica MD05G1091700.v1.1
prunus_persica Prupe.8G131100_v2.0.a1
pyrus_communis pycom05g08580 pycom14g01040
rosa_chinensis RchiOBHm_Chr6g0255871
rosa_laevigata RLG00000014807
rosa_multiflora Rmu_sc0001738.1_g000001 Rmu_sc0001738.1_g000002
rosa_roxburghii Rroxscaffold_7G00209580
rosa_rugosa Rorug05G0500200 Rorug05G0561900
rosa_samantha Rh6AG080200 Rh6CG005900 Rh6CG071000 Rh6DG006100 Rh6DG068300
rosa_wichuraiana Rw6G006920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 497
Acc16I TGCGCA 1 cut(s) 352
AccI GTMKAC 1 cut(s) 681
AclWI GGATC 2 cut(s) 635, 915
AcoI YGGCCR 2 cut(s) 424, 587
AcsI RAATTY 1 cut(s) 931
AcvI CACGTG 1 cut(s) 692
AfaI GTAC 3 cut(s) 222, 506, 518
AfiI CCNNNNNNNGG 1 cut(s) 532
AflIII ACRYGT 2 cut(s) 300, 510
AgsI TTSAA 1 cut(s) 948
AjiI CACGTC 1 cut(s) 511
AjnI CCWGG 1 cut(s) 567
AluBI AGCT 1 cut(s) 915
AluI AGCT 1 cut(s) 915
Alw26I GTCTC 1 cut(s) 466
AlwI GGATC 2 cut(s) 635, 915
Ama87I CYCGRG 1 cut(s) 497
AoxI GGCC 6 cut(s) 379, 424, 477, 587, 685, 750
ApoI RAATTY 1 cut(s) 931
ArsI GACNNNNNNTTYG 4 cut(s) 272, 304, 555, 587
AspLEI GCGC 1 cut(s) 353
AspS9I GGNCC 5 cut(s) 478, 535, 592, 686, 751
AsuC2I CCSGG 3 cut(s) 714, 754, 848
AsuHPI GGTGA 6 cut(s) 124, 145, 331, 376, 727, 927
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 2 cut(s) 535, 592
BauI CACGAG 1 cut(s) 389
BbrPI CACGTG 1 cut(s) 692
BccI CCATC 2 cut(s) 185, 946
BceAI ACGGC 4 cut(s) 224, 439, 447, 700
BcgI CGANNNNNNTGC 2 cut(s) 352, 386
BciT130I CCWGG 1 cut(s) 569
BcnI CCSGG 3 cut(s) 714, 754, 848
BcoDI GTCTC 1 cut(s) 466
BisI GCNGC 2 cut(s) 97, 427
BlsI GCNGC 2 cut(s) 98, 428
Bme1390I CCNGG 4 cut(s) 569, 714, 754, 848
Bme18I GGWCC 2 cut(s) 535, 592
BmeT110I CYCGRG 1 cut(s) 497
BmgBI CACGTC 1 cut(s) 511
BmgT120I GGNCC 5 cut(s) 478, 535, 592, 686, 751
BmiI GGNNCC 1 cut(s) 119
BmrFI CCNGG 4 cut(s) 569, 714, 754, 848
BoxI GACNNNNGTC 1 cut(s) 527
BpuMI CCSGG 3 cut(s) 714, 754, 848
Bsa29I ATCGAT 1 cut(s) 441
BsaAI YACGTR 2 cut(s) 520, 692
BsaBI GATNNNNATC 1 cut(s) 626
BsaJI CCNNGG 3 cut(s) 567, 662, 713
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 213, 243
Bsc4I CCNNNNNNNGG 1 cut(s) 532
Bse118I RCCGGY 2 cut(s) 585, 589
Bse8I GATNNNNATC 1 cut(s) 626
BseBI CCWGG 1 cut(s) 569
BseCI ATCGAT 1 cut(s) 441
BseDI CCNNGG 3 cut(s) 567, 662, 713
BseGI GGATG 4 cut(s) 54, 177, 673, 726
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 1 cut(s) 532
BseRI GAGGAG 3 cut(s) 50, 110, 190
BseX3I CGGCCG 2 cut(s) 424, 587
Bsh1285I CGRYCG 2 cut(s) 427, 590
BshFI GGCC 6 cut(s) 381, 426, 479, 589, 687, 752
BshVI ATCGAT 1 cut(s) 441
BsiEI CGRYCG 2 cut(s) 427, 590
BsiHKCI CYCGRG 1 cut(s) 497
BsiSI CCGG 7 cut(s) 311, 586, 590, 713, 741, 754, 847
BsiWI CGTACG 2 cut(s) 504, 516
BslFI GGGAC 3 cut(s) 289, 379, 644
BslI CCNNNNNNNGG 1 cut(s) 532
BsmAI GTCTC 1 cut(s) 466
BsmBI CGTCTC 1 cut(s) 466
BsmFI GGGAC 3 cut(s) 289, 379, 644
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 6 cut(s) 381, 426, 479, 589, 687, 752
BsoBI CYCGRG 1 cut(s) 497
Bsp143I GATC 3 cut(s) 621, 627, 907
BspANI GGCC 6 cut(s) 381, 426, 479, 589, 687, 752
BspDI ATCGAT 1 cut(s) 441
BspHI TCATGA 1 cut(s) 796
BspLI GGNNCC 1 cut(s) 119
BspPI GGATC 2 cut(s) 635, 915
BsrFI RCCGGY 2 cut(s) 585, 589
BssAI RCCGGY 2 cut(s) 585, 589
BssECI CCNNGG 3 cut(s) 567, 662, 713
BssMI GATC 3 cut(s) 621, 627, 907
BssSI CACGAG 1 cut(s) 389
BssT1I CCWWGG 1 cut(s) 662
Bst2BI CACGAG 1 cut(s) 389
Bst2UI CCWGG 1 cut(s) 569
Bst6I CTCTTC 2 cut(s) 128, 404
BstBAI YACGTR 2 cut(s) 520, 692
BstC8I GCNNGC 3 cut(s) 237, 477, 587
BstF5I GGATG 4 cut(s) 54, 177, 673, 726
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 3 cut(s) 624, 630, 910
BstMAI GTCTC 1 cut(s) 466
BstMBI GATC 3 cut(s) 621, 627, 907
BstMCI CGRYCG 2 cut(s) 427, 590
BstMWI GCNNNNNNNGC 2 cut(s) 102, 765
BstNI CCWGG 1 cut(s) 569
BstNSI RCATGY 1 cut(s) 304
BstPAI GACNNNNGTC 1 cut(s) 527
BstSCI CCNGG 4 cut(s) 567, 712, 752, 846
BstZI CGGCCG 2 cut(s) 424, 587
Bsu15I ATCGAT 1 cut(s) 441
BsuRI GGCC 6 cut(s) 381, 426, 479, 589, 687, 752
BsuTUI ATCGAT 1 cut(s) 441
BtrI CACGTC 1 cut(s) 511
BtsCI GGATG 4 cut(s) 54, 177, 673, 726
Cac8I GCNNGC 3 cut(s) 237, 477, 587
CciI TCATGA 1 cut(s) 796
CfoI GCGC 1 cut(s) 353
Cfr10I RCCGGY 2 cut(s) 585, 589
Cfr13I GGNCC 5 cut(s) 478, 535, 592, 686, 751
ClaI ATCGAT 1 cut(s) 441
CseI GACGC 1 cut(s) 257
Csp6I GTAC 3 cut(s) 221, 505, 517
CviAII CATG 3 cut(s) 301, 785, 797
CviQI GTAC 3 cut(s) 221, 505, 517
DpnI GATC 3 cut(s) 623, 629, 909
DpnII GATC 3 cut(s) 621, 627, 907
EaeI YGGCCR 2 cut(s) 424, 587
EagI CGGCCG 2 cut(s) 424, 587
Eam1104I CTCTTC 2 cut(s) 128, 404
EarI CTCTTC 2 cut(s) 128, 404
EciI GGCGGA 3 cut(s) 114, 322, 733
EclXI CGGCCG 2 cut(s) 424, 587
Eco130I CCWWGG 1 cut(s) 662
Eco47I GGWCC 2 cut(s) 535, 592
Eco52I CGGCCG 2 cut(s) 424, 587
Eco72I CACGTG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 497
EcoRII CCWGG 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 662
ErhI CCWWGG 1 cut(s) 662
Esp3I CGTCTC 1 cut(s) 466
FaeI CATG 3 cut(s) 304, 788, 800
FaiI YATR 7 cut(s) 302, 326, 650, 652, 786, 798, 912
FaqI GGGAC 3 cut(s) 289, 379, 644
FatI CATG 3 cut(s) 300, 784, 796
FauI CCCGC 4 cut(s) 240, 244, 315, 350
FblI GTMKAC 1 cut(s) 681
Fnu4HI GCNGC 2 cut(s) 97, 427
FokI GGATG 4 cut(s) 41, 164, 680, 733
Fsp4HI GCNGC 2 cut(s) 97, 427
FspI TGCGCA 1 cut(s) 352
GlaI GCGC 1 cut(s) 352
GluI GCNGC 2 cut(s) 97, 427
HaeIII GGCC 6 cut(s) 381, 426, 479, 589, 687, 752
HapII CCGG 7 cut(s) 311, 586, 590, 713, 741, 754, 847
HgaI GACGC 1 cut(s) 257
HhaI GCGC 1 cut(s) 353
Hin1II CATG 3 cut(s) 304, 788, 800
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HincII GTYRAC 2 cut(s) 555, 682
HindII GTYRAC 2 cut(s) 555, 682
HinfI GANTC 3 cut(s) 392, 468, 673
HpaII CCGG 7 cut(s) 311, 586, 590, 713, 741, 754, 847
HphI GGTGA 6 cut(s) 124, 145, 331, 376, 727, 927
Hpy166II GTNNAC 2 cut(s) 555, 682
Hpy188I TCNGA 1 cut(s) 613
Hpy188III TCNNGA 4 cut(s) 465, 595, 625, 797
Hpy8I GTNNAC 2 cut(s) 555, 682
Hpy99I CGWCG 4 cut(s) 251, 512, 686, 836
HpyAV CCTTC 1 cut(s) 145
HpyCH4IV ACGT 3 cut(s) 510, 519, 691
HpyCH4V TGCA 3 cut(s) 363, 618, 927
HpyF10VI GCNNNNNNNGC 2 cut(s) 102, 765
HpySE526I ACGT 3 cut(s) 510, 519, 691
Hsp92II CATG 3 cut(s) 304, 788, 800
HspAI GCGC 1 cut(s) 351
KroI GCCGGC 1 cut(s) 585
KroNI GCCGGC 1 cut(s) 587
Kzo9I GATC 3 cut(s) 621, 627, 907
LmnI GCTCC 2 cut(s) 123, 756
MaeII ACGT 3 cut(s) 510, 519, 691
MaeIII GTNAC 1 cut(s) 520
MalI GATC 3 cut(s) 623, 629, 909
MboI GATC 3 cut(s) 621, 627, 907
MboII GAAGA 6 cut(s) 115, 118, 121, 248, 391, 936
MfeI CAATTG 1 cut(s) 545
MluCI AATT 3 cut(s) 364, 545, 931
MlyI GAGTC 1 cut(s) 401
MroNI GCCGGC 1 cut(s) 585
MseI TTAA 1 cut(s) 967
MspI CCGG 7 cut(s) 311, 586, 590, 713, 741, 754, 847
MspR9I CCNGG 4 cut(s) 569, 714, 754, 848
MunI CAATTG 1 cut(s) 545
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 1 cut(s) 569
MwoI GCNNNNNNNGC 2 cut(s) 102, 765
NaeI GCCGGC 1 cut(s) 587
NciI CCSGG 3 cut(s) 714, 754, 848
NdeII GATC 3 cut(s) 621, 627, 907
NgoMIV GCCGGC 1 cut(s) 585
NlaIII CATG 3 cut(s) 304, 788, 800
NlaIV GGNNCC 1 cut(s) 119
NmuCI GTSAC 1 cut(s) 520
NsbI TGCGCA 1 cut(s) 352
NspI RCATGY 1 cut(s) 304
PaeR7I CTCGAG 1 cut(s) 497
PagI TCATGA 1 cut(s) 796
PciI ACATGT 1 cut(s) 300
PcsI WCGNNNNNNNCGW 4 cut(s) 447, 504, 516, 630
PctI GAATGC 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 587
PfeI GAWTC 2 cut(s) 468, 673
Pfl23II CGTACG 2 cut(s) 504, 516
PkrI GCNGC 2 cut(s) 98, 428
PleI GAGTC 1 cut(s) 400
PmaCI CACGTG 1 cut(s) 692
PmlI CACGTG 1 cut(s) 692
PpsI GAGTC 1 cut(s) 400
Ppu21I YACGTR 2 cut(s) 520, 692
PscI ACATGT 1 cut(s) 300
PshAI GACNNNNGTC 1 cut(s) 527
Psp6I CCWGG 1 cut(s) 567
PspCI CACGTG 1 cut(s) 692
PspGI CCWGG 1 cut(s) 567
PspLI CGTACG 2 cut(s) 504, 516
PspN4I GGNNCC 1 cut(s) 119
PspPI GGNCC 5 cut(s) 478, 535, 592, 686, 751
PspXI VCTCGAGB 1 cut(s) 497
RsaI GTAC 3 cut(s) 222, 506, 518
RsaNI GTAC 3 cut(s) 221, 505, 517
SalI GTCGAC 1 cut(s) 680
SaqAI TTAA 1 cut(s) 967
SatI GCNGC 2 cut(s) 97, 427
Sau3AI GATC 3 cut(s) 621, 627, 907
Sau96I GGNCC 5 cut(s) 478, 535, 592, 686, 751
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 4 cut(s) 569, 714, 754, 848
Sfr274I CTCGAG 1 cut(s) 497
SinI GGWCC 2 cut(s) 535, 592
SlaI CTCGAG 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 3 cut(s) 364, 545, 931
StyD4I CCNGG 4 cut(s) 567, 712, 752, 846
StyI CCWWGG 1 cut(s) 662
TaiI ACGT 3 cut(s) 513, 522, 694
TaqI TCGA 6 cut(s) 246, 441, 471, 498, 681, 806
TasI AATT 3 cut(s) 364, 545, 931
TauI GCSGC 2 cut(s) 99, 429
TfiI GAWTC 2 cut(s) 468, 673
Tru1I TTAA 1 cut(s) 967
Tru9I TTAA 1 cut(s) 967
TseFI GTSAC 1 cut(s) 520
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 4 cut(s) 17, 261, 427, 785
TspGWI ACGGA 2 cut(s) 208, 693
VpaK11BI GGWCC 2 cut(s) 535, 592
XapI RAATTY 1 cut(s) 931
XceI RCATGY 1 cut(s) 304
XhoI CTCGAG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.