Rorug05G0500200

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013606
Physical Location & Seq
Reverse (-)
68149630 .. 68150781
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0500200.1

Sequence Viewer

Length: 1152 bp
ATGGCAGCTGAAGAAACTAAAAAACCAGAGGCTGCTGCCATTGACAATGTTGCAGCCATTTTTGGGGTAACTGGGTTAGTTGGGAAGGTACTTGCCAGAAAGCTAATATCAACCCCCAAATGGAAGGTTTATGGTATAGCTCGAGCTCCTGATCATCAGATCATACCCATTGATCAAAGTTCTTCAAACTTCCATTTCATCCAATGTGATTTGCTAAACCCATTGGAAACCCAGAAAAGACTCCCATTGTTACAAGATGTGACTCATGTCTTTTGGGTCACTTGGGCAAGCCAGTACCAATTGGATAGCATTGAGTGCTGTGACCAAAACAGAGCCATGATGTCCAATGCATTGGATGCTATACTTCCTGGTGCCATGGCATTGAGGCATGTTTCTCTTCAGACTGGGATGAAGCATTATGTGTCACTGCAAGGCCCTTTTGATGAAACTAAAGAAGCTCGATACTACAATGAGGAGTCTCCGCGAGTAAATCCAAGCTACAACTTTTACTATGTTCTTGAAGACTTGCTCAAGGAGAGGCTACATGGTCAGATAGGCTGGTCTGTGCATAGGCCTGGTTTGTTAATCGGTAGTTCTTCTAGGACTTTGTACAATTTTATGGGTAGTTTATGTGTTTATGGGACTATTTGTAAGCATTTAAATCTCCCTTTTGTGTTTGGAGGGACAAAAAAGTGTTGGGAAGAGACTTGTATTGATGGCTCAGATGCAAGGCTAGTAGCTGATCAACACATTTGGGCAGCCACAAATGTTGATTTGGATGATCATAGCAATTTGTTTAATGCGATCAACGGTCCAAGTTTTACATGGAAGGAGATCTGGAAAGCTGTTGCAGAAAAGCTTGGAGTACAAGTATCAGAAGAACACACATTTTCTGAGGACTTCTGGTATTCAAAAGCCATGGCTGACAAGCAACAAGTGTGGGAACAAATTGTAGCAGAAAAAGGGCTGGTTGAAACTGAGATGGACAATTTGGCCAATTGGGATTTCTTGGATGTTCTGTTTCGCTGCCCGGTTAAGATTTTGGGGACTCGAGAAAAAGTTGATGGACTTGGTTTTACTGTAAGGTACAAGACAGTGGATGCAATTATGTATTGGATAGATTGTATGAGAGATGAGAAACTAATACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

383

Amino Acids

43.57

Weight (kDa)

5.51

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 76 - 334 4.9e-13 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013606)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05560
malus_domestica MD05G1091700.v1.1
prunus_persica Prupe.8G131100_v2.0.a1
pyrus_communis pycom05g08580 pycom14g01040
rosa_chinensis RchiOBHm_Chr6g0255871
rosa_laevigata RLG00000014807
rosa_multiflora Rmu_sc0001738.1_g000001 Rmu_sc0001738.1_g000002
rosa_roxburghii Rroxscaffold_7G00209580
rosa_rugosa Rorug05G0500200 Rorug05G0561900
rosa_samantha Rh6AG080200 Rh6CG005900 Rh6CG071000 Rh6DG006100 Rh6DG068300
rosa_wichuraiana Rw6G006920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 371
AccII CGCG 1 cut(s) 484
AciI CCGC 1 cut(s) 482
AcoI YGGCCR 1 cut(s) 993
AcuI CTGAAG 2 cut(s) 30, 383
AfaI GTAC 5 cut(s) 90, 296, 611, 867, 1088
AfiI CCNNNNNNNGG 2 cut(s) 63, 120
AgsI TTSAA 4 cut(s) 186, 521, 912, 974
AjnI CCWGG 2 cut(s) 367, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 989, 1021
AluBI AGCT 9 cut(s) 8, 103, 140, 146, 458, 498, 740, 845, 859
AluI AGCT 9 cut(s) 8, 103, 140, 146, 458, 498, 740, 845, 859
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 2 cut(s) 483, 698
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 2 cut(s) 141, 1050
AoxI GGCC 3 cut(s) 433, 572, 993
ApeKI GCWGC 6 cut(s) 5, 32, 35, 53, 758, 1026
AspS9I GGNCC 2 cut(s) 434, 812
AsuC2I CCSGG 1 cut(s) 1031
AvaI CYCGRG 2 cut(s) 141, 1050
AvaII GGWCC 1 cut(s) 812
BalI TGGCCA 1 cut(s) 995
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 6 cut(s) 17, 19, 22, 65, 770, 1013
BccI CCATC 3 cut(s) 710, 976, 1058
BciT130I CCWGG 2 cut(s) 369, 576
BclI TGATCA 4 cut(s) 151, 172, 742, 781
BcnI CCSGG 1 cut(s) 1031
BcoDI GTCTC 2 cut(s) 483, 698
BfaI CTAG 2 cut(s) 600, 734
BglII AGATCT 1 cut(s) 834
BisI GCNGC 6 cut(s) 6, 33, 36, 54, 759, 1027
BlsI GCNGC 6 cut(s) 7, 34, 37, 55, 760, 1028
Bme1390I CCNGG 3 cut(s) 369, 576, 1031
Bme18I GGWCC 1 cut(s) 812
BmeT110I CYCGRG 2 cut(s) 141, 1050
BmgT120I GGNCC 2 cut(s) 434, 812
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 3 cut(s) 369, 576, 1031
BmrI ACTGGG 2 cut(s) 81, 414
BmsI GCATC 3 cut(s) 346, 715, 1090
BmuI ACTGGG 2 cut(s) 81, 414
BoxI GACNNNNGTC 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 1031
BsaJI CCNNGG 2 cut(s) 375, 918
BsaXI ACNNNNNCTCC 2 cut(s) 855, 885
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 120
Bse1I ACTGG 3 cut(s) 76, 292, 409
BseBI CCWGG 2 cut(s) 369, 576
BseDI CCNNGG 2 cut(s) 375, 918
BseGI GGATG 6 cut(s) 198, 361, 414, 784, 1018, 1105
BseLI CCNNNNNNNGG 2 cut(s) 63, 120
BseMII CTCAG 3 cut(s) 735, 885, 969
BseNI ACTGG 3 cut(s) 76, 292, 409
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 6 cut(s) 17, 19, 22, 65, 770, 1013
Bsh1236I CGCG 1 cut(s) 484
BshFI GGCC 3 cut(s) 435, 574, 995
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 1 cut(s) 148
BsiHKCI CYCGRG 2 cut(s) 141, 1050
BsiSI CCGG 1 cut(s) 1031
BslFI GGGAC 3 cut(s) 655, 697, 1060
BslI CCNNNNNNNGG 2 cut(s) 63, 120
BsmAI GTCTC 2 cut(s) 483, 698
BsmFI GGGAC 3 cut(s) 655, 697, 1060
BsnI GGCC 3 cut(s) 435, 574, 995
BsoBI CYCGRG 2 cut(s) 141, 1050
Bsp1286I GDGCHC 1 cut(s) 148
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
Bsp19I CCATGG 2 cut(s) 375, 918
BspACI CCGC 1 cut(s) 482
BspANI GGCC 3 cut(s) 435, 574, 995
BspCNI CTCAG 3 cut(s) 734, 886, 970
BspFNI CGCG 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 3 cut(s) 76, 292, 409
BssECI CCNNGG 2 cut(s) 375, 918
BssMI GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
BssT1I CCWWGG 2 cut(s) 375, 918
Bst2UI CCWGG 2 cut(s) 369, 576
Bst4CI ACNGT 3 cut(s) 812, 1081, 1096
Bst6I CTCTTC 2 cut(s) 402, 696
BstAPI GCANNNNNTGC 2 cut(s) 315, 356
BstAUI TGTACA 1 cut(s) 609
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 3 cut(s) 721, 894, 978
BstDSI CCRYGG 2 cut(s) 375, 918
BstF5I GGATG 6 cut(s) 198, 361, 414, 784, 1018, 1105
BstFNI CGCG 1 cut(s) 484
BstKTI GATC 7 cut(s) 154, 162, 175, 745, 784, 807, 837
BstMAI GTCTC 2 cut(s) 483, 698
BstMBI GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
BstMWI GCNNNNNNNGC 2 cut(s) 315, 356
BstNI CCWGG 2 cut(s) 369, 576
BstNSI RCATGY 1 cut(s) 392
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 3 cut(s) 367, 574, 1029
BstUI CGCG 1 cut(s) 484
BstV1I GCAGC 6 cut(s) 17, 19, 22, 65, 770, 1013
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 1 cut(s) 834
BstXI CCANNNNNNTGG 1 cut(s) 352
BstYI RGATCY 1 cut(s) 834
BsuRI GGCC 3 cut(s) 435, 574, 995
BtgI CCRYGG 2 cut(s) 375, 918
BtsCI GGATG 6 cut(s) 198, 361, 414, 784, 1018, 1105
BtsI GCAGTG 1 cut(s) 425
BtsIMutI CAGTG 2 cut(s) 425, 1101
Cac8I GCNNGC 1 cut(s) 289
CaiI CAGNNNCTG 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 434, 812
Csp6I GTAC 5 cut(s) 89, 295, 610, 866, 1087
CspCI CAANNNNNGTGG 4 cut(s) 751, 786, 920, 955
CviAII CATG 7 cut(s) 266, 337, 376, 389, 545, 825, 919
CviQI GTAC 5 cut(s) 89, 295, 610, 866, 1087
DdeI CTNAG 3 cut(s) 721, 894, 978
DpnI GATC 7 cut(s) 153, 161, 174, 744, 783, 806, 836
DpnII GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
DraI TTTAAA 1 cut(s) 660
EaeI YGGCCR 1 cut(s) 993
Eam1104I CTCTTC 2 cut(s) 402, 696
EarI CTCTTC 2 cut(s) 402, 696
Ecl136II GAGCTC 1 cut(s) 146
Eco130I CCWWGG 2 cut(s) 375, 918
Eco147I AGGCCT 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 812
Eco53kI GAGCTC 1 cut(s) 146
Eco57I CTGAAG 2 cut(s) 30, 383
Eco88I CYCGRG 2 cut(s) 141, 1050
EcoICRI GAGCTC 1 cut(s) 146
EcoO109I RGGNCCY 1 cut(s) 434
EcoRII CCWGG 2 cut(s) 367, 574
EcoT14I CCWWGG 2 cut(s) 375, 918
EcoT22I ATGCAT 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 375, 918
FaeI CATG 7 cut(s) 269, 340, 379, 392, 548, 828, 922
FaqI GGGAC 3 cut(s) 655, 697, 1060
FatI CATG 7 cut(s) 265, 336, 375, 388, 544, 824, 918
FbaI TGATCA 4 cut(s) 151, 172, 742, 781
Fnu4HI GCNGC 6 cut(s) 6, 33, 36, 54, 759, 1027
FokI GGATG 6 cut(s) 185, 368, 421, 791, 1025, 1112
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 6 cut(s) 6, 33, 36, 54, 759, 1027
FspBI CTAG 2 cut(s) 600, 734
GluI GCNGC 6 cut(s) 6, 33, 36, 54, 759, 1027
HaeIII GGCC 3 cut(s) 435, 574, 995
HapII CCGG 1 cut(s) 1031
Hin1II CATG 7 cut(s) 269, 340, 379, 392, 548, 828, 922
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 4 cut(s) 240, 262, 476, 1048
HpaII CCGG 1 cut(s) 1031
Hpy188I TCNGA 6 cut(s) 159, 402, 552, 724, 877, 895
Hpy188III TCNNGA 4 cut(s) 149, 518, 838, 1052
HpyAV CCTTC 3 cut(s) 79, 118, 823
HpyCH4III ACNGT 3 cut(s) 812, 1081, 1096
HpyCH4V TGCA 7 cut(s) 53, 350, 430, 568, 728, 851, 1103
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 356
HpyF3I CTNAG 3 cut(s) 721, 894, 978
Hsp92II CATG 7 cut(s) 269, 340, 379, 392, 548, 828, 922
Ksp22I TGATCA 4 cut(s) 151, 172, 742, 781
Kzo9I GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
LmnI GCTCC 1 cut(s) 151
Lsp1109I GCAGC 6 cut(s) 17, 19, 22, 65, 770, 1013
LweI GCATC 3 cut(s) 346, 715, 1090
MaeI CTAG 2 cut(s) 600, 734
MaeIII GTNAC 6 cut(s) 67, 249, 259, 277, 320, 423
MalI GATC 7 cut(s) 153, 161, 174, 744, 783, 806, 836
MboI GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
MboII GAAGA 7 cut(s) 23, 174, 389, 533, 588, 713, 890
MfeI CAATTG 2 cut(s) 299, 997
MflI RGATCY 1 cut(s) 834
MhlI GDGCHC 1 cut(s) 148
MlsI TGGCCA 1 cut(s) 995
MluCI AATT 7 cut(s) 299, 613, 790, 948, 988, 997, 1104
MluNI TGGCCA 1 cut(s) 995
MlyI GAGTC 4 cut(s) 234, 256, 485, 1042
MnlI CCTC 6 cut(s) 22, 378, 466, 531, 674, 889
Mox20I TGGCCA 1 cut(s) 995
Mph1103I ATGCAT 1 cut(s) 352
MscI TGGCCA 1 cut(s) 995
MseI TTAA 5 cut(s) 584, 659, 798, 1035, 1150
Msp20I TGGCCA 1 cut(s) 995
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 1031
MspR9I CCNGG 3 cut(s) 369, 576, 1031
MunI CAATTG 2 cut(s) 299, 997
MvaI CCWGG 2 cut(s) 369, 576
MvnI CGCG 1 cut(s) 484
MwoI GCNNNNNNNGC 2 cut(s) 315, 356
NciI CCSGG 1 cut(s) 1031
NcoI CCATGG 2 cut(s) 375, 918
NdeII GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
NlaIII CATG 7 cut(s) 269, 340, 379, 392, 548, 828, 922
NlaIV GGNNCC 1 cut(s) 373
NmuCI GTSAC 4 cut(s) 259, 277, 320, 423
NsiI ATGCAT 1 cut(s) 352
NspI RCATGY 1 cut(s) 392
PaeR7I CTCGAG 2 cut(s) 141, 1050
PceI AGGCCT 1 cut(s) 574
PkrI GCNGC 6 cut(s) 7, 34, 37, 55, 760, 1028
PleI GAGTC 4 cut(s) 234, 256, 484, 1042
PpsI GAGTC 4 cut(s) 234, 256, 484, 1042
PshAI GACNNNNGTC 1 cut(s) 266
Psp124BI GAGCTC 1 cut(s) 148
Psp6I CCWGG 2 cut(s) 367, 574
PspGI CCWGG 2 cut(s) 367, 574
PspN4I GGNNCC 1 cut(s) 373
PspPI GGNCC 2 cut(s) 434, 812
PspXI VCTCGAGB 1 cut(s) 141
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 1 cut(s) 834
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 5 cut(s) 90, 296, 611, 867, 1088
RsaNI GTAC 5 cut(s) 89, 295, 610, 866, 1087
SacI GAGCTC 1 cut(s) 148
SaqAI TTAA 5 cut(s) 584, 659, 798, 1035, 1150
SatI GCNGC 6 cut(s) 6, 33, 36, 54, 759, 1027
Sau3AI GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
Sau96I GGNCC 2 cut(s) 434, 812
SchI GAGTC 4 cut(s) 234, 256, 485, 1042
ScrFI CCNGG 3 cut(s) 369, 576, 1031
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 3 cut(s) 346, 715, 1090
Sfr274I CTCGAG 2 cut(s) 141, 1050
SinI GGWCC 1 cut(s) 812
SlaI CTCGAG 2 cut(s) 141, 1050
SmiI ATTTAAAT 1 cut(s) 660
SmlI CTYRAG 3 cut(s) 141, 530, 1050
SmoI CTYRAG 3 cut(s) 141, 530, 1050
Sse9I AATT 7 cut(s) 299, 613, 790, 948, 988, 997, 1104
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 1 cut(s) 482
SspMI CTAG 2 cut(s) 600, 734
SstI GAGCTC 1 cut(s) 148
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 3 cut(s) 367, 574, 1029
StyI CCWWGG 2 cut(s) 375, 918
SwaI ATTTAAAT 1 cut(s) 660
TaaI ACNGT 3 cut(s) 812, 1081, 1096
TaqI TCGA 3 cut(s) 142, 460, 1051
TasI AATT 7 cut(s) 299, 613, 790, 948, 988, 997, 1104
TatI WGTACW 2 cut(s) 609, 865
Tru1I TTAA 5 cut(s) 584, 659, 798, 1035, 1150
Tru9I TTAA 5 cut(s) 584, 659, 798, 1035, 1150
TscAI CASTG 2 cut(s) 432, 1101
TseFI GTSAC 4 cut(s) 259, 277, 320, 423
TseI GCWGC 6 cut(s) 5, 32, 35, 53, 758, 1026
Tsp45I GTSAC 4 cut(s) 259, 277, 320, 423
TspDTI ATGAA 3 cut(s) 187, 425, 459
TspRI CASTG 2 cut(s) 432, 1101
VpaK11BI GGWCC 1 cut(s) 812
XceI RCATGY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 822
XhoI CTCGAG 2 cut(s) 141, 1050
XspI CTAG 2 cut(s) 600, 734
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.