Rroxscaffold_7G00209580

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013606
Physical Location & Seq
Forward (+)
59777900 .. 59778814
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00209580.1

Sequence Viewer

Length: 915 bp
ATGCCGCCGCTTCCGTCTCCCACCGCCTATGGCTCCTCTTCTTCTTCACCTTCTTCACTCTCGCTTTCGCCCTCGCCCTCATCAACACCACCCTCCCCAACCCCACCTCTCTCTCCCCCACCTCCACTATCCCCCTCCCGCCCTCCGTCGCCGACGCGCTTCTCCCACTACGCCATTTCCTCCAACGCCTCATCCGCATCGTCCCACATGTCCCTCTCCGAAATCACAGCCATCTCCACGGCCCTGACTGCCTGCTCGCACAGGTGTAATTTCCTCGTCTTTGGCCTCACCCACGAGTCCCTCCTCTGGCACGCGCTCAATCTTAACGGCCGTACCGTCTTCCTCGACGACAATGAGTTCCTCATCTCCAAGTTCGAACAGTCACACCCCTACCACGGAATCGAAGCGTACGACGTATCGTTCGCCACCACGGTCAAGGAATTCAGGTCCCTCCTCTCCGCCACCAAGTCTCAAGTCCAAAACGACTGTCGTCCGGTGCAGAACTTGCTGTTTTCCGACTGCAAGCTCGGGATCAACGACTTGCCGAATCACATATACCAAGTCCCTTGGGATGTGATTTTGGTCGACGGGCCAAGTGGGTATTCTCCGTCGTCGCCGGGTCGGATGGCTCCGATTTTCACCGCCGGAGTTTTGGCCCGGAGCAAGAAGGGCGGCGGGGACAAGACCCATGTTTTTGTTCACGATTTTTCTAGAGAGGTTGAGAAGATTAGTAGCGACGAGTTTCTTTGCCGGGAAAACCTGGTGCAGGCTGTCGATTTGTTGGGCCATTTTGTTTTGGAGAAAATGGAAGATAACAAATTTGAGTTTTGTAGAAACTCGACATCATCATCATCATCACGATCTTCGTCTAAATCATCAGGGAATGAAGACGGTGATGATGACGAAGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

304

Amino Acids

32.96

Weight (kDa)

5.41

Isoelectric Point (pI)

73.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 74 - 264 2.1e-76 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013606)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05560
malus_domestica MD05G1091700.v1.1
prunus_persica Prupe.8G131100_v2.0.a1
pyrus_communis pycom05g08580 pycom14g01040
rosa_chinensis RchiOBHm_Chr6g0255871
rosa_laevigata RLG00000014807
rosa_multiflora Rmu_sc0001738.1_g000001 Rmu_sc0001738.1_g000002
rosa_roxburghii Rroxscaffold_7G00209580
rosa_rugosa Rorug05G0500200 Rorug05G0561900
rosa_samantha Rh6AG080200 Rh6CG005900 Rh6CG071000 Rh6DG006100 Rh6DG068300
rosa_wichuraiana Rw6G006920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 585
AccII CGCG 2 cut(s) 157, 314
AciI CCGC 9 cut(s) 5, 8, 24, 139, 195, 459, 642, 672, 675
AclWI GGATC 1 cut(s) 539
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 2 cut(s) 440, 818
AfaI GTAC 2 cut(s) 334, 410
AfiI CCNNNNNNNGG 3 cut(s) 306, 395, 766
AflIII ACRYGT 1 cut(s) 207
AjnI CCWGG 1 cut(s) 759
AjuI GAANNNNNNNTTGG 2 cut(s) 586, 618
AluBI AGCT 1 cut(s) 526
AluI AGCT 1 cut(s) 526
Alw26I GTCTC 2 cut(s) 21, 474
AlwI GGATC 1 cut(s) 539
Ama87I CYCGRG 1 cut(s) 527
AoxI GGCC 6 cut(s) 240, 283, 328, 590, 654, 784
ApoI RAATTY 2 cut(s) 440, 818
ArsI GACNNNNNNTTYG 4 cut(s) 404, 436, 472, 504
AspLEI GCGC 2 cut(s) 159, 316
AspS9I GGNCC 5 cut(s) 241, 447, 590, 655, 784
AsuC2I CCSGG 3 cut(s) 618, 658, 752
AsuHPI GGTGA 4 cut(s) 39, 280, 631, 905
AsuII TTCGAA 1 cut(s) 375
AvaI CYCGRG 1 cut(s) 527
AvaII GGWCC 1 cut(s) 447
BaeI ACNNNNGTAYC 2 cut(s) 316, 349
BauI CACGAG 1 cut(s) 293
BbsI GAAGAC 2 cut(s) 331, 894
BccI CCATC 2 cut(s) 239, 619
BceAI ACGGC 3 cut(s) 255, 315, 343
BciT130I CCWGG 1 cut(s) 761
BcnI CCSGG 3 cut(s) 618, 658, 752
BcoDI GTCTC 2 cut(s) 21, 474
BfaI CTAG 1 cut(s) 711
BisI GCNGC 3 cut(s) 5, 8, 673
BlsI GCNGC 3 cut(s) 6, 9, 674
Bme1390I CCNGG 4 cut(s) 618, 658, 752, 761
Bme18I GGWCC 1 cut(s) 447
BmeT110I CYCGRG 1 cut(s) 527
BmgT120I GGNCC 5 cut(s) 241, 447, 590, 655, 784
BmiI GGNNCC 3 cut(s) 34, 449, 630
BmrFI CCNGG 4 cut(s) 618, 658, 752, 761
BmsI GCATC 1 cut(s) 206
BoxI GACNNNNGTC 1 cut(s) 489
BpiI GAAGAC 2 cut(s) 331, 894
Bpu14I TTCGAA 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 456
BpuMI CCSGG 3 cut(s) 618, 658, 752
BsaJI CCNNGG 4 cut(s) 237, 394, 429, 566
BsaWI WCCGGW 1 cut(s) 493
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 395, 766
BseBI CCWGG 1 cut(s) 761
BseDI CCNNGG 4 cut(s) 237, 394, 429, 566
BseGI GGATG 3 cut(s) 191, 577, 630
BseLI CCNNNNNNNGG 3 cut(s) 306, 395, 766
BseRI GAGGAG 3 cut(s) 25, 293, 443
BseX3I CGGCCG 1 cut(s) 328
BsgI GTGCAG 2 cut(s) 518, 785
Bsh1236I CGCG 2 cut(s) 157, 314
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 6 cut(s) 242, 285, 330, 592, 656, 786
BsiEI CGRYCG 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 527
BsiSI CCGG 5 cut(s) 494, 617, 645, 658, 751
BsiWI CGTACG 1 cut(s) 408
BslFI GGGAC 6 cut(s) 187, 196, 283, 433, 548, 692
BslI CCNNNNNNNGG 3 cut(s) 306, 395, 766
BsmAI GTCTC 2 cut(s) 21, 474
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 6 cut(s) 187, 196, 283, 433, 548, 692
BsnI GGCC 6 cut(s) 242, 285, 330, 592, 656, 786
BsoBI CYCGRG 1 cut(s) 527
Bsp119I TTCGAA 1 cut(s) 375
Bsp143I GATC 2 cut(s) 531, 860
BspACI CCGC 9 cut(s) 5, 8, 24, 139, 195, 459, 642, 672, 675
BspANI GGCC 6 cut(s) 242, 285, 330, 592, 656, 786
BspFNI CGCG 2 cut(s) 157, 314
BspLI GGNNCC 3 cut(s) 34, 449, 630
BspPI GGATC 1 cut(s) 539
BspT104I TTCGAA 1 cut(s) 375
BssECI CCNNGG 4 cut(s) 237, 394, 429, 566
BssMI GATC 2 cut(s) 531, 860
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 1 cut(s) 566
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 761
Bst4CI ACNGT 5 cut(s) 337, 381, 433, 488, 893
Bst6I CTCTTC 1 cut(s) 43
BstAPI GCANNNNNTGC 1 cut(s) 505
BstBI TTCGAA 1 cut(s) 375
BstC8I GCNNGC 5 cut(s) 253, 257, 312, 524, 768
BstDSI CCRYGG 3 cut(s) 237, 394, 429
BstENI CCTNNNNNAGG 1 cut(s) 764
BstF5I GGATG 3 cut(s) 191, 577, 630
BstFNI CGCG 2 cut(s) 157, 314
BstHHI GCGC 2 cut(s) 159, 316
BstKTI GATC 2 cut(s) 534, 863
BstMAI GTCTC 2 cut(s) 21, 474
BstMBI GATC 2 cut(s) 531, 860
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 4 cut(s) 194, 248, 505, 669
BstNI CCWGG 1 cut(s) 761
BstNSI RCATGY 1 cut(s) 211
BstPAI GACNNNNGTC 1 cut(s) 489
BstSCI CCNGG 4 cut(s) 616, 656, 750, 759
BstUI CGCG 2 cut(s) 157, 314
BstV2I GAAGAC 2 cut(s) 331, 894
BstZI CGGCCG 1 cut(s) 328
BsuRI GGCC 6 cut(s) 242, 285, 330, 592, 656, 786
BtgI CCRYGG 3 cut(s) 237, 394, 429
BtsCI GGATG 3 cut(s) 191, 577, 630
Cac8I GCNNGC 5 cut(s) 253, 257, 312, 524, 768
CfoI GCGC 2 cut(s) 159, 316
Cfr13I GGNCC 5 cut(s) 241, 447, 590, 655, 784
CseI GACGC 1 cut(s) 163
CsiI ACCWGGT 1 cut(s) 759
Csp6I GTAC 2 cut(s) 333, 409
CviAII CATG 2 cut(s) 208, 689
CviQI GTAC 2 cut(s) 333, 409
DpnI GATC 2 cut(s) 533, 862
DpnII GATC 2 cut(s) 531, 860
EaeI YGGCCR 1 cut(s) 328
EagI CGGCCG 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 43
EarI CTCTTC 1 cut(s) 43
EciI GGCGGA 1 cut(s) 448
EclXI CGGCCG 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 566
Eco47I GGWCC 1 cut(s) 447
Eco52I CGGCCG 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 527
EcoNI CCTNNNNNAGG 1 cut(s) 764
EcoO109I RGGNCCY 1 cut(s) 447
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 759
EcoT14I CCWWGG 1 cut(s) 566
ErhI CCWWGG 1 cut(s) 566
Esp3I CGTCTC 1 cut(s) 21
FaeI CATG 2 cut(s) 211, 692
FaiI YATR 5 cut(s) 30, 209, 554, 556, 690
FaqI GGGAC 6 cut(s) 187, 196, 283, 433, 548, 692
FatI CATG 2 cut(s) 207, 688
FauI CCCGC 2 cut(s) 146, 668
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 3 cut(s) 5, 8, 673
FokI GGATG 3 cut(s) 178, 584, 637
Fsp4HI GCNGC 3 cut(s) 5, 8, 673
FspBI CTAG 1 cut(s) 711
GlaI GCGC 2 cut(s) 158, 315
GluI GCNGC 3 cut(s) 5, 8, 673
HaeIII GGCC 6 cut(s) 242, 285, 330, 592, 656, 786
HapII CCGG 5 cut(s) 494, 617, 645, 658, 751
HgaI GACGC 1 cut(s) 163
HhaI GCGC 2 cut(s) 159, 316
Hin1II CATG 2 cut(s) 211, 692
Hin6I GCGC 2 cut(s) 157, 314
HinP1I GCGC 2 cut(s) 157, 314
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HinfI GANTC 3 cut(s) 296, 399, 547
HpaII CCGG 5 cut(s) 494, 617, 645, 658, 751
HphI GGTGA 4 cut(s) 39, 280, 631, 905
Hpy166II GTNNAC 2 cut(s) 586, 700
Hpy188I TCNGA 4 cut(s) 220, 517, 624, 633
Hpy188III TCNNGA 4 cut(s) 529, 701, 711, 858
Hpy8I GTNNAC 2 cut(s) 586, 700
Hpy99I CGWCG 8 cut(s) 151, 157, 350, 416, 590, 613, 616, 740
HpyAV CCTTC 2 cut(s) 60, 661
HpyCH4III ACNGT 5 cut(s) 337, 381, 433, 488, 893
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 3 cut(s) 499, 522, 766
HpyF10VI GCNNNNNNNGC 4 cut(s) 194, 248, 505, 669
HpySE526I ACGT 1 cut(s) 414
Hsp92II CATG 2 cut(s) 211, 692
HspAI GCGC 2 cut(s) 157, 314
Kzo9I GATC 2 cut(s) 531, 860
LmnI GCTCC 3 cut(s) 38, 634, 660
LweI GCATC 1 cut(s) 206
MabI ACCWGGT 1 cut(s) 759
MaeI CTAG 1 cut(s) 711
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 381
MalI GATC 2 cut(s) 533, 862
MboI GATC 2 cut(s) 531, 860
MboII GAAGA 9 cut(s) 30, 33, 36, 45, 331, 736, 821, 855, 899
MluCI AATT 3 cut(s) 268, 440, 818
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 3 cut(s) 207, 540, 602
MseI TTAA 2 cut(s) 324, 913
MspI CCGG 5 cut(s) 494, 617, 645, 658, 751
MspR9I CCNGG 4 cut(s) 618, 658, 752, 761
MvaI CCWGG 1 cut(s) 761
MvnI CGCG 2 cut(s) 157, 314
MwoI GCNNNNNNNGC 4 cut(s) 194, 248, 505, 669
NciI CCSGG 3 cut(s) 618, 658, 752
NdeII GATC 2 cut(s) 531, 860
NlaIII CATG 2 cut(s) 211, 692
NlaIV GGNNCC 3 cut(s) 34, 449, 630
NmuCI GTSAC 1 cut(s) 381
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 1 cut(s) 375
PciI ACATGT 1 cut(s) 207
PcsI WCGNNNNNNNCGW 3 cut(s) 333, 408, 534
PfeI GAWTC 2 cut(s) 399, 547
Pfl23II CGTACG 1 cut(s) 408
PkrI GCNGC 3 cut(s) 6, 9, 674
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 447
PscI ACATGT 1 cut(s) 207
PshAI GACNNNNGTC 1 cut(s) 489
Psp5II RGGWCCY 1 cut(s) 447
Psp6I CCWGG 1 cut(s) 759
PspGI CCWGG 1 cut(s) 759
PspLI CGTACG 1 cut(s) 408
PspN4I GGNNCC 3 cut(s) 34, 449, 630
PspPI GGNCC 5 cut(s) 241, 447, 590, 655, 784
PspPPI RGGWCCY 1 cut(s) 447
RsaI GTAC 2 cut(s) 334, 410
RsaNI GTAC 2 cut(s) 333, 409
SalI GTCGAC 1 cut(s) 584
SaqAI TTAA 2 cut(s) 324, 913
SatI GCNGC 3 cut(s) 5, 8, 673
Sau3AI GATC 2 cut(s) 531, 860
Sau96I GGNCC 5 cut(s) 241, 447, 590, 655, 784
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 4 cut(s) 618, 658, 752, 761
SetI ASST 9 cut(s) 52, 109, 124, 266, 417, 449, 528, 720, 762
SexAI ACCWGGT 1 cut(s) 759
SfaNI GCATC 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 375
SinI GGWCC 1 cut(s) 447
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
Sse9I AATT 3 cut(s) 268, 440, 818
SsiI CCGC 9 cut(s) 5, 8, 24, 139, 195, 459, 642, 672, 675
SspMI CTAG 1 cut(s) 711
StyD4I CCNGG 4 cut(s) 616, 656, 750, 759
StyI CCWWGG 1 cut(s) 566
TaaI ACNGT 5 cut(s) 337, 381, 433, 488, 893
TaiI ACGT 1 cut(s) 417
TaqI TCGA 6 cut(s) 345, 375, 402, 585, 774, 839
TasI AATT 3 cut(s) 268, 440, 818
TauI GCSGC 3 cut(s) 7, 10, 675
TfiI GAWTC 2 cut(s) 399, 547
Tru1I TTAA 2 cut(s) 324, 913
Tru9I TTAA 2 cut(s) 324, 913
TseFI GTSAC 1 cut(s) 381
Tsp45I GTSAC 1 cut(s) 381
TspDTI ATGAA 1 cut(s) 900
TspGWI ACGGA 3 cut(s) 135, 411, 597
VpaK11BI GGWCC 1 cut(s) 447
XagI CCTNNNNNAGG 1 cut(s) 764
XapI RAATTY 2 cut(s) 440, 818
XbaI TCTAGA 1 cut(s) 710
XceI RCATGY 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 585
XspI CTAG 1 cut(s) 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.