pycom05g10410

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005708
Physical Location & Seq
Reverse (-)
13767211 .. 13790172
22962 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6267 bp
ATGGCTGGTGCTTTGGATGGGGAACTTGTCCACTATTTGCCGACAATAACGTCTCATTTACGTGATGCGATTGCTCCACGTAGAGGTAGACCCTCTCTTGAGGCTTTGGCTTGTGTTGGAAACATAGCAAAAGCAATGGGGCCAGCAATGGAACCTCATGTTCGTGGTCTTTTGGATGTTATGTTGTCTGCTGGTCTTTCCCCTACGCTTGTTGAGGCACTTGAGCATATAACTACCAGTATTCCATCTTTGCTTCCTACTATCCAAGATCGGTTGCTTGACAGCATTTCGGTGGTTCTTTCAAAATCCCACCATTCTCAGGGAAGGTCGGCCGTTGGCATGGGCAGAGGAAAATTAATGAATATCCCTCAGCAAGTCTCAGACCTCAGTGGTTCAGCCCTTGTGCAGCTTGCTTTGCAGACTCTAGCTCGTTTTAATTTCAAGGGCCATGACCTTCTTGATTTTGCAAGAGAATCAGTTGTTGTATATTTGGATGATGATGATGGAGCCATCCGGAAAGATGCTGCCCTATGTTGTTGCAAATTAGTTGCAAATTCCTTTTCTGGTGTGCAATATGCTTCTGATAGGTCTAATCGGGGAAAACGACGACGTCTTGTTGAGGAGATTGTGGAAAAGCTTCTCATTGAAGCTGTTGCAGACGCTGATGTTATTGTTCGCCATTCAATATTTTCCTCACTTCATGGGAATAGAGGTTTTGATGATTTTTTGGCGCAGGCTGATAGTCTGAGCGCAGTTTTTGCTGCTTTGAATGATGAGGATTTTGATGTTCGAGAGTTTGCAATTTCTGTGGCTGGGAGATTGTCGGAAAAAAATCCTGCTTATGTTCTTCCAGCACTTCGTCGGCATCTCATACAATTGTTGACTTACCTGGAGCAGAGTGCAGACACCAAATGCCGAGAAGAAAGTGCGAAGTTACTGGGTTGTTTAATACGAAACTGTGAACGACTAATACTTCCATACATTGCTCCGATACACAAGGCACTTGTTGCAAGGCTTACTGATGGCACTGGTGTGAACGCAAATAATGGCATTATTAGTGGAGTTCTTGTAACTGTTGGGGATCTGGCTAGAGTGGGTGGTTATGCAATGCGAATATACATTCCTGAGCTTATGCCTTTAATTGTTGAGGCATTATTGGATGGAACAGCTGTCACCAAACGGGAAGTGGCTGTCGCTACTCTTGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACGCCATACAATGAATATCCACTGTTACTTGGCTTACTCTTGAAGTTACTGAATGGTGAATTAGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTCGGAATTATGGGTGCTCTAGACCCTCATGTGCATAAACGACATCAGCAAAGCCTGCCCGGTTCACATGGAGATGTTGCATGTTGTGCCAATGAATCTGGTCAACACATTCAATCTGTGGATGAATTGCCTATGGACCCGTGGCCATCATTTGCAACGCCTGAAGACTATTATTCTACGGTTGCAATCAACTCCCTTATGCGTATACTTAGGGACCCTTCACTTGCTACTTACCACCTGAACGTGGTCAGATCTCTTATGTTCATATTCAAGTCAATGGGTGTTGGTTGTGTTCCATACTTACCAAAGGTTTTACCTGATTTCTTCCATATAGTCCGGACATGTGATGATGCTTTAAAGGATTTTATAACATGGAAGCTTGGTACGCTAGTGTCTATTGTGCGTCAGCATATTGGGAAATATCTGCATGAGTTGCTCGTTCTAATTTCAGAATTATGGTCAACCTTCAGTTTTCCTGCTGCTGGCCGTCCTCAACTTGGTTATCCAGTTCTGCATCTGGTGGAGCAGCTTTGCTTGGCTCTCAATGATGAATTTCGAACGTACCTTCCGGATATTCTTCCATGTTGTATTCAAGTTCTAAGTGATGCAGAACGGTATAACGATTATACTTATGTTCTTGACATCCTCCATACCCTTGAAGTGTTTGGTGGAACATTGGATGAACATATGCATCTTCTTCTTCCTGCACTCATTCGCTTGTTTAAAGTTGATGCCTCAGTGGACATAAGACGTGCTGCTATTAAAACTTTGATGAAACTGATCCCTCGTGTGCAGGTTACTGGTCACATATCTTCTCTCGTGCATCACTTAAAGCTAGTCTTAGATGGCAAGAATGATGAGCTCCGGAAGGATGCTGTTGATGCCCTTTATTGTCTAGCTCATGCCCTTGGGGAGGACTTCACAATTTTTATCCCATCAATACACAAACTTCTGCTGAGACATCGCTTAAGGCATAAGGAATTCGAGGAAATTGAAGGATGGTTGAAGCAACATGAACCTCTAATTTTGGGGAGTACAACTTTTCAAAGATTAAGCCGACGGCTTCCAGCAGAGGTTATTACTGACAATTGGAATGATTTAGAGACTGACCCTTATGATGTTGGGTCAGATGTGCAGAAACAACTTCGAGGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAGCGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACATTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCTTTACGAACCTGTGCAAGGCTTGCACAGCTGCAGCCATTTGTTGGGCGGGAGCTATTTGCAGCTGGTTTTGTTAGCTGTTGGGCACAGCTAAATGAGACTAGTGAGAAACAGTTAGTTGGAAGTTTGGAGATGGCATTTTCATCCCCAAATTTACCTCCTGAAATTTTGGCAACACTTCTTAACTTGGCAGAGTTCATGGAACACAATGAGAAGCCTCTTCCTATAGACATTCGCCTGCTTGGTGCTCTTGCAGAAAAGTGTCGTGCATTTGCAAAGGCCTTGCATTACAAAGAAATGGAATTTGAAGGAGCGCGCTCCAAGAAAATGGACCCTGTTGCTGTAGTTGAAGATCTTATACATATAAACAACCAGTTACACCAGCATGAGGCTGCAGTTGGAATATTGACCTTTGCCCAACTAGATTTGGATGTTCAACTCAAAGAGTCATGGTATGAGAAGTTGCAGCGATGGGACGATGCTCTCAAGGCCTACACTGAAAAAGCCTCTCAAGCATCGAGTCCACGTCTTGTTTTGGATGCCGCTTTAGGTCGGATGCGATGTCTTGCTGCTTTGGCACGATGGGAGGAGCTTAACAACCTGTTCAAGGAATATTGGACCCCAGCTGAACCGGCTGCTCGGTTGGAAATGGCATCAATGGCTGCTAATGCTGCTTGGAACATGGGGGAGTGGGATCAAATGGCAGAATACGTGTCTCGGTTAGATGATGGTGATGAAACCAAACTTAGGGGCTTGGGGAACACTGCTGCTAGTGGGGATGGAAGTGGTAATGGAACATTCTTTAGAGCTGTTCTGTTAGTTCGAAGAGGAAAGTATGATGAAGCACGTGAGTATGTTGAGAGAGCTAGGAAATGCTTGGCAACAGAGCTCGCTGCTTTGGTTTTGGAGAGCTATGAACGTGCATATGGCAATATGGTCCGTGTTCAGCAACTGTCAGAACTTGAGGAGGTCATTGATTATTGCACACTTCCCCTGGGAAACCCTGTTGCTGAAGGACGACGAGCACTTATTCGCAATATGTGGAATGAGCGGATACAAGGAGCAAAACGTAATGTTGAGGTATGGCAGGCGCTTTTAGCAGTTAGAGCACTTGTTCTACCTCCAACAGAAGATATTGACACTTGGCTTAAGTATGCTTACCTTTGTCGGAAAAGTGGGCGAATTAGTCAGGCTAGATCTACTTTAGTCGAACTCTTACAGTATGATCCTGAAACATCTCCTGAAAGCGTGCGGTATGATGGGCCCCCACAAGTGATGCTTGCATATTTGGAATACCAATGGTCACTTGGAGAGGACATAAAGCGCAAGGAAGCATTTGCTAGGCTACAGAATTTGGCAGTAGAGCTTTCAAGTCGACCTAGTATTCAACCGGTTACCCCGTCTGGCTTGATGAGCTCTAGTAGTCTGAGTGTTCCACTTGTCGCCCGTGTGCATCTCATACTGGGAGACTGGAATTGGGCTCTTTCTCCTGGATTGGATGATGATTCTATACAAGAAATTCTCAATGCCTTCCGGACTGCAACTCAATGTGCCAATAAATGGGCAAGGGCATGGCACAAGTGGGCACTGTTTAATACAGCAGTGATGTCACTTTATAATGCAAGAGGTTGTGCAAATGTCGCGTCACAGTTTGTTGTTGCAGCGGTCACTGGATATTTTCATTCGATAGCATGTTCAGCGAATACTAAAGGTGTTGATGATAGTTTACAGGATATTCTTCGTCTTCTTACATTGTGGTTCAACCATGGAGCTACTGCAGAAGTGCAAATGGCACTACAGAAAGGGTTTGCACATGTTAATATTAATACATGGCTGGTGGTTTTGCCTCAAATAATCGCCAGGATACATTCTAATACCAATGCTGTGAGGGAACTGATACATTCGCTTTTGGTTCGGATTGGACAAAGTCATCCACAGGCTCTTATGTACCCTCTCCTTGTAGCATGTAAATCAATAAGTAATTTACGTAAAGCTGCAGCTCAAGAAGTGGTTGACAAAGTTCGGCAGCATAGTGGTGTGCTTGTGGACCAGGCACAGCTTGTGTCAAAGGAACTAATCCGGGTCGCAATACTTTGGCATGAAAAGTGGCATGAGGGTCTGGAAGAAGCTAGTCGCCTGTATTTTGGTGAACATAACATTGAGGGAATGCTAAAAGAATTGGAGCAACTCCATGAAATGCTTGAGGAAGGAGCTAAAGAGAACAATACAACCATAAAAGAGAAAGCATTCATTGAGGCATATCGTCATGATTTGCTGGAGGCCTATGAGTGCTGCATGAAATTTAAGAGAACAGGGAAAGATGCTGAGCTTACTCGGGCTTGGGATGTTTACTATCAGGTATTTAGACGAATAGATAAGCAGCTGCAGAGTCTTACTACTCTGGACTTAGAGTCCGTCTCACCAGAGTTGTTGGAATGTCGTAATTTGGAGCTCGCTGTTCCTGGGAGATATCGTGCGGAATCTCCTGTTGTAACAATTGCATCGTTTGCATGCCAACTTGTTGTCATAACATCCAAACAAAGACCAAGAAAATTGACGATTCGTGGGAGTGATGGTAAGGATTATGCCTTCTTGCTGAAGGGACATGAAGATTTACGCCAAGATGAACGCGTCATGCAGCTTTTTTGTTTGGTGAATACACTACTGGAGAATTCAACAAAAACTGAGGAGAAGGATCTCTCCATTGAACGATATGATGTCATTCCATTGTCTTCAAACAGTGGACTAATTGGTTGGGTTCCAAAATGTGACACCCTTAACCAGCTCATCAGGGAGTACAGAGATGCCAGAAAGATCACTCTAAATCAAGAGCACAAGTATTTGCTAAGTTTTGCTCCGGATTATGATCGTTTGCCACTCATAGCTAAAGTGGAAGTCTTTGAGTATGCCCTGCACAATACAGAGGGAAACGACCTTGCAAGGGTCCTTTGGCTGAAAAGTCGCACTTCTGAGGTATGGCTGGAGAGGAGGACAAACTATACGAGAAGTTTAGCAGTTATGAGCATGGTTGGGTACCTTCTTGGCTTAGGTGATCGGCACCCAAGCAATCTTATGCTGAACCGCTACAGTGGGAAGATCTTGCACATTGATTTTGGGGACTGCTTTGAGGCGTCTATGAACCGGGAAAAGTTCCCTGAGAAGGTCCCCTTTCGGTTGACTAGAATGCTTGTGAAAGCAATGGGGGTTAGTGGTATAGAGGGCAACTTTCGTTCAACATGTCAAAATGTGATGGAAGTCCTACGAATACATAAAGATAGCGTTATGGCTATGATGGAGGCCTTTGTCCATGATCCTCTCATCAATTGGCGTCTCTTCAACTTCAATGAAGTTCCCCAAATGTCAGTGCTTGGAAATAGTAATGTTACTCCTGTTGTGGGTGCTGAAGAACCTGCTCAACATAGAGAGCTTCGCCAACCTCAACGAGGTGCTCGTGAAAGGGAACTTCTTCAGGCTGTCAATCAACTGGGTGATGCTAATGAGGTCTTGAATGAGCTTGCTGTGGTTGTCATGGCTCGGATGAAGAGTAAACTTACTGGACGTGATTTTTCTGCCAGTTCCTCTGTATCTTCCAGCTCTATTCAACATGCAATTGACCACAGCACGCTGATTTCTGGAGACAGTAGAGAAGTTGATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0001558 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005844 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009303 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009733 GO:0009743 GO:0009744 GO:0009745 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010115 GO:0010116 GO:0010154 GO:0010182 GO:0010243 GO:0010467 GO:0010468 GO:0010506 GO:0010507 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0016070 GO:0016072 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019216 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019747 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030258 GO:0030307 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035004 GO:0036092 GO:0036211 GO:0040008 GO:0040019 GO:0042221 GO:0042493 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0043621 GO:0043900 GO:0043901 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0045017 GO:0045472 GO:0045828 GO:0045834 GO:0045893 GO:0045927 GO:0045935 GO:0045995 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046677 GO:0046834 GO:0046854 GO:0046889 GO:0046890 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050687 GO:0050688 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0052742 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071704 GO:0080090 GO:0080134 GO:0090069 GO:0090070 GO:0090304 GO:0090407 GO:0097159 GO:0097305 GO:0097327 GO:0097659 GO:0098781 GO:0140096 GO:1900457 GO:1900459 GO:1901355 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901654 GO:1901698 GO:1901700 GO:1901701 GO:1902659 GO:1902661 GO:1902680 GO:1902930 GO:1902932 GO:1903506 GO:1903508 GO:1990904 GO:2000026 GO:2000112 GO:2000232 GO:2000234 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04072 ko04136 ko04138 ko04139 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04218 ko04371 ko04630 ko04659 ko04714 ko04910 ko04919 ko04920 ko04930 ko04931 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05212 ko05214 ko05215 ko05221 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04012 map04066 map04072 map04136 map04138 map04139 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04218 map04371 map04630 map04659 map04714 map04910 map04919 map04920 map04930 map04931 map05165 map05167 map05200 map05205 map05206 map05210 map05212 map05214 map05215 map05221 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
DUF3385 FAT FATC FRB_dom PI3_PI4_kinase

Protein Analysis

2089

Amino Acids

234.57

Weight (kDa)

6.2

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTOR_dom PF11865 411 - 579 2.7e-51 Serine/threonine-protein kinase mTOR domain
FAT PF02259 1087 - 1453 1.4e-91 FAT domain
HEAT_ATR PF23593 1468 - 1547 9.9e-11 Serine/threonine-protein kinase ATR-like, HEAT repeats
FRB_dom PF08771 1560 - 1662 3.5e-36 FKBP12-rapamycin binding domain
PI3_PI4_kinase PF00454 1731 - 1979 1.1e-77 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1700, 4224
AasI GACNNNNNNGTC 1 cut(s) 49
AatII GACGTC 1 cut(s) 613
Acc36I ACCTGC 2 cut(s) 2116, 6025
Acc65I GGTACC 1 cut(s) 5640
AccB1I GGYRCC 2 cut(s) 5640, 5664
AccB7I CCANNNNNTGG 2 cut(s) 2651, 4167
AccBSI CCGCTC 1 cut(s) 3658
AccI GTMKAC 4 cut(s) 88, 1307, 1537, 3982
AccII CGCG 3 cut(s) 2923, 4250, 5238
AccIII TCCGGA 6 cut(s) 513, 1668, 1900, 2196, 4140, 5464
AciI CCGC 7 cut(s) 2656, 3150, 3658, 3859, 4271, 5084, 5689
AclWI GGATC 6 cut(s) 1089, 2107, 3309, 3827, 5310, 5912
AcoI YGGCCR 3 cut(s) 330, 1475, 1816
AcuI CTGAAG 7 cut(s) 1515, 1783, 2609, 3639, 5225, 6030, 6059
AcvI CACGTG 1 cut(s) 3455
AcyI GRCGYC 3 cut(s) 610, 5738, 5935
AfaI GTAC 9 cut(s) 1215, 1323, 1717, 1895, 2368, 2539, 4556, 5405, 5642
AflII CTTAAG 2 cut(s) 2299, 3755
AflIII ACRYGT 5 cut(s) 1673, 3318, 4420, 5236, 5843
AgeI ACCGGT 1 cut(s) 3997
AhdI GACNNNNNGTC 1 cut(s) 5017
AhlI ACTAGT 1 cut(s) 2708
AjiI CACGTC 4 cut(s) 1316, 2084, 3134, 6167
AjnI CCWGG 6 cut(s) 888, 3600, 4096, 4466, 4656, 5068
AleI CACNNNNGTG 1 cut(s) 1031
AloI GAACNNNNNNTCC 2 cut(s) 1882, 1914
AlwI GGATC 6 cut(s) 1089, 2107, 3309, 3827, 5310, 5912
AlwNI CAGNNNCTG 2 cut(s) 662, 3559
Ama87I CYCGRG 1 cut(s) 4941
Aor13HI TCCGGA 6 cut(s) 513, 1668, 1900, 2196, 4140, 5464
ApaI GGGCCC 1 cut(s) 3873
ArsI GACNNNNNNTTYG 2 cut(s) 4235, 4267
AseI ATTAAT 2 cut(s) 356, 4431
AsiGI ACCGGT 1 cut(s) 3997
Asp700I GAANNNNTTC 3 cut(s) 636, 4853, 6072
Asp718I GGTACC 1 cut(s) 5640
AspLEI GCGC 7 cut(s) 733, 752, 2535, 2923, 2925, 3700, 3933
AsuC2I CCSGG 3 cut(s) 1392, 4688, 5750
AsuHPI GGTGA 8 cut(s) 1165, 1304, 3350, 4766, 5019, 5272, 5669, 6107
AsuII TTCGAA 2 cut(s) 1888, 3430
AvaI CYCGRG 1 cut(s) 4941
AvaII GGWCC 8 cut(s) 1468, 1546, 2938, 3225, 3544, 4654, 5551, 5770
BaeGI GKGCMC 3 cut(s) 2695, 3873, 4195
BaeI ACNNNNGTAYC 2 cut(s) 1707, 1740
BalI TGGCCA 1 cut(s) 1477
BanI GGYRCC 2 cut(s) 5640, 5664
BanII GRGCYC 6 cut(s) 2196, 3498, 3873, 4025, 4090, 5061
BarI GAAGNNNNNNTAC 2 cut(s) 2949, 2981
BauI CACGAG 3 cut(s) 2118, 2150, 6057
BbrPI CACGTG 1 cut(s) 3455
BbsI GAAGAC 3 cut(s) 1503, 4343, 5331
BbvCI CCTCAGC 1 cut(s) 369
BceAI ACGGC 3 cut(s) 317, 1803, 2408
BciT130I CCWGG 6 cut(s) 890, 3602, 4098, 4468, 4658, 5070
BciVI GTATCC 2 cut(s) 3654, 4464
BcnI CCSGG 3 cut(s) 1392, 4688, 5750
BcuI ACTAGT 1 cut(s) 2708
BfoI RGCGCY 1 cut(s) 3701
BfrI CTTAAG 2 cut(s) 2299, 3755
BfuAI ACCTGC 2 cut(s) 2116, 6025
BfuI GTATCC 2 cut(s) 3654, 4464
BglII AGATCT 4 cut(s) 1583, 2959, 3803, 5703
BlpI GCTNAGC 1 cut(s) 4932
BmcAI AGTACT 1 cut(s) 1323
Bme1390I CCNGG 9 cut(s) 890, 1392, 3602, 4098, 4468, 4658, 4688, 5070, 5750
Bme18I GGWCC 8 cut(s) 1468, 1546, 2938, 3225, 3544, 4654, 5551, 5770
BmeRI GACNNNNNGTC 1 cut(s) 5017
BmeT110I CYCGRG 1 cut(s) 4941
BmgBI CACGTC 4 cut(s) 1316, 2084, 3134, 6167
BmrFI CCNGG 9 cut(s) 890, 1392, 3602, 4098, 4468, 4658, 4688, 5070, 5750
BmrI ACTGGG 4 cut(s) 947, 1233, 4079, 6101
BmuI ACTGGG 4 cut(s) 947, 1233, 4079, 6101
BpiI GAAGAC 3 cut(s) 1503, 4343, 5331
BplI GAGNNNNNCTC 2 cut(s) 5009, 5041
BpmI CTGGAG 5 cut(s) 911, 4904, 5294, 5609, 6261
Bpu10I CCTNAGC 3 cut(s) 369, 1125, 5653
Bpu1102I GCTNAGC 1 cut(s) 4932
Bpu14I TTCGAA 2 cut(s) 1888, 3430
BpuEI CTTGAG 7 cut(s) 119, 242, 3077, 3102, 3590, 4593, 4829
BpuMI CCSGG 3 cut(s) 1392, 4688, 5750
Bsa29I ATCGAT 1 cut(s) 6262
BsaAI YACGTR 5 cut(s) 62, 80, 3319, 3455, 4595
BsaBI GATNNNNATC 1 cut(s) 5472
BsaHI GRCGYC 3 cut(s) 610, 5738, 5935
BsaJI CCNNGG 6 cut(s) 1472, 2239, 3600, 3601, 4372, 5069
BsaWI WCCGGW 7 cut(s) 513, 1668, 1900, 2196, 3997, 4140, 5464
BsaXI ACNNNNNCTCC 6 cut(s) 1053, 1083, 5065, 5095, 5167, 5197
Bse118I RCCGGY 2 cut(s) 3238, 3997
Bse3DI GCAATG 5 cut(s) 141, 153, 981, 1113, 5811
Bse8I GATNNNNATC 1 cut(s) 5472
BseAI TCCGGA 6 cut(s) 513, 1668, 1900, 2196, 4140, 5464
BseBI CCWGG 6 cut(s) 890, 3602, 4098, 4468, 4658, 5070
BseCI ATCGAT 1 cut(s) 6262
BseDI CCNNGG 6 cut(s) 1472, 2239, 3600, 3601, 4372, 5069
BseJI GATNNNNATC 1 cut(s) 5472
BseMI GCAATG 5 cut(s) 141, 153, 981, 1113, 5811
BsePI GCGCGC 1 cut(s) 2921
BseRI GAGGAG 5 cut(s) 635, 3209, 3587, 5309, 5608
BseSI GKGCMC 3 cut(s) 2695, 3873, 4195
BseX3I CGGCCG 1 cut(s) 330
BseYI CCCAGC 3 cut(s) 812, 2516, 3229
BsgI GTGCAG 6 cut(s) 425, 921, 2022, 2144, 2486, 5504
Bsh1236I CGCG 3 cut(s) 2923, 4250, 5238
Bsh1285I CGRYCG 1 cut(s) 333
BshNI GGYRCC 2 cut(s) 5640, 5664
BshTI ACCGGT 1 cut(s) 3997
BshVI ATCGAT 1 cut(s) 6262
BsiEI CGRYCG 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 4941
BslFI GGGAC 5 cut(s) 1559, 3095, 5223, 5738, 5756
BsmBI CGTCTC 3 cut(s) 57, 5029, 5942
BsmFI GGGAC 5 cut(s) 1559, 3095, 5223, 5738, 5756
BsmI GAATGC 3 cut(s) 4779, 4853, 5796
BsoBI CYCGRG 1 cut(s) 4941
Bsp119I TTCGAA 2 cut(s) 1888, 3430
Bsp120I GGGCCC 1 cut(s) 3869
Bsp13I TCCGGA 6 cut(s) 513, 1668, 1900, 2196, 4140, 5464
Bsp1407I TGTACA 1 cut(s) 1213
Bsp1720I GCTNAGC 1 cut(s) 4932
Bsp19I CCATGG 1 cut(s) 4372
BspACI CCGC 7 cut(s) 2656, 3150, 3658, 3859, 4271, 5084, 5689
BspDI ATCGAT 1 cut(s) 6262
BspEI TCCGGA 6 cut(s) 513, 1668, 1900, 2196, 4140, 5464
BspFNI CGCG 3 cut(s) 2923, 4250, 5238
BspHI TCATGA 1 cut(s) 4873
BspMAI CTGCAG 5 cut(s) 2643, 3004, 4387, 4606, 4995
BspMI ACCTGC 2 cut(s) 2116, 6025
BspPI GGATC 6 cut(s) 1089, 2107, 3309, 3827, 5310, 5912
BspT104I TTCGAA 2 cut(s) 1888, 3430
BspT107I GGYRCC 2 cut(s) 5640, 5664
BspTI CTTAAG 2 cut(s) 2299, 3755
BsrBI CCGCTC 1 cut(s) 3658
BsrDI GCAATG 5 cut(s) 141, 153, 981, 1113, 5811
BsrFI RCCGGY 2 cut(s) 3238, 3997
BsrGI TGTACA 1 cut(s) 1213
BssAI RCCGGY 2 cut(s) 3238, 3997
BssECI CCNNGG 6 cut(s) 1472, 2239, 3600, 3601, 4372, 5069
BssHII GCGCGC 1 cut(s) 2921
BssNAI GTATAC 1 cut(s) 1538
BssNI GRCGYC 3 cut(s) 610, 5738, 5935
BssSI CACGAG 3 cut(s) 2118, 2150, 6057
BssT1I CCWWGG 2 cut(s) 2239, 4372
Bst1107I GTATAC 1 cut(s) 1538
Bst2BI CACGAG 3 cut(s) 2118, 2150, 6057
Bst2UI CCWGG 6 cut(s) 890, 3602, 4098, 4468, 4658, 5070
Bst6I CTCTTC 4 cut(s) 2832, 3427, 5945, 6143
BstACI GRCGYC 3 cut(s) 610, 5738, 5935
BstAFI CTTAAG 2 cut(s) 2299, 3755
BstAPI GCANNNNNTGC 7 cut(s) 758, 1007, 1387, 1418, 1765, 2630, 5114
BstAUI TGTACA 1 cut(s) 1213
BstBAI YACGTR 5 cut(s) 62, 80, 3319, 3455, 4595
BstBI TTCGAA 2 cut(s) 1888, 3430
BstDSI CCRYGG 2 cut(s) 1472, 4372
BstEII GGTNACC 1 cut(s) 4000
BstENI CCTNNNNNAGG 4 cut(s) 2243, 2623, 3212, 6048
BstFNI CGCG 3 cut(s) 2923, 4250, 5238
BstH2I RGCGCY 1 cut(s) 3701
BstHHI GCGC 7 cut(s) 733, 752, 2535, 2923, 2925, 3700, 3933
BstMCI CGRYCG 1 cut(s) 333
BstNI CCWGG 6 cut(s) 890, 3602, 4098, 4468, 4658, 5070
BstNSI RCATGY 8 cut(s) 1416, 1677, 4302, 4424, 4575, 5121, 5847, 6215
BstPI GGTNACC 1 cut(s) 4000
BstSCI CCNGG 9 cut(s) 888, 1390, 3600, 4096, 4466, 4656, 4686, 5068, 5748
BstSLI GKGCMC 3 cut(s) 2695, 3873, 4195
BstSNI TACGTA 1 cut(s) 4595
BstUI CGCG 3 cut(s) 2923, 4250, 5238
BstV2I GAAGAC 3 cut(s) 1503, 4343, 5331
BstX2I RGATCY 6 cut(s) 1081, 1583, 2959, 3803, 5302, 5703
BstXI CCANNNNNNTGG 1 cut(s) 2935
BstYI RGATCY 6 cut(s) 1081, 1583, 2959, 3803, 5302, 5703
BstZ17I GTATAC 1 cut(s) 1538
BstZI CGGCCG 1 cut(s) 330
Bsu15I ATCGAT 1 cut(s) 6262
BsuI GTATCC 2 cut(s) 3654, 4464
BsuTUI ATCGAT 1 cut(s) 6262
BtgI CCRYGG 2 cut(s) 1472, 4372
BtgZI GCGATG 3 cut(s) 2279, 3091, 3181
BtrI CACGTC 4 cut(s) 1316, 2084, 3134, 6167
BtsI GCAGTG 3 cut(s) 2511, 3369, 4215
BveI ACCTGC 2 cut(s) 2116, 6025
CaiI CAGNNNCTG 2 cut(s) 662, 3559
CciI TCATGA 1 cut(s) 4873
CfoI GCGC 7 cut(s) 733, 752, 2535, 2923, 2925, 3700, 3933
Cfr10I RCCGGY 2 cut(s) 3238, 3997
ClaI ATCGAT 1 cut(s) 6262
CseI GACGC 6 cut(s) 668, 1724, 4239, 5227, 5727, 5924
Csp6I GTAC 9 cut(s) 1214, 1322, 1716, 1894, 2367, 2538, 4555, 5404, 5641
CspAI ACCGGT 1 cut(s) 3997
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviQI GTAC 9 cut(s) 1214, 1322, 1716, 1894, 2367, 2538, 4555, 5404, 5641
DraI TTTAAA 2 cut(s) 1689, 2056
DrdI GACNNNNNNGTC 1 cut(s) 49
DriI GACNNNNNGTC 1 cut(s) 5017
DseDI GACNNNNNNGTC 1 cut(s) 49
EaeI YGGCCR 3 cut(s) 330, 1475, 1816
EagI CGGCCG 1 cut(s) 330
Eam1104I CTCTTC 4 cut(s) 2832, 3427, 5945, 6143
Eam1105I GACNNNNNGTC 1 cut(s) 5017
EarI CTCTTC 4 cut(s) 2832, 3427, 5945, 6143
Ecl136II GAGCTC 4 cut(s) 2194, 3496, 4023, 5059
EclXI CGGCCG 1 cut(s) 330
Eco105I TACGTA 1 cut(s) 4595
Eco130I CCWWGG 2 cut(s) 2239, 4372
Eco147I AGGCCT 4 cut(s) 2888, 3098, 4889, 5906
Eco24I GRGCYC 6 cut(s) 2196, 3498, 3873, 4025, 4090, 5061
Eco32I GATATC 1 cut(s) 5078
Eco47I GGWCC 8 cut(s) 1468, 1546, 2938, 3225, 3544, 4654, 5551, 5770
Eco52I CGGCCG 1 cut(s) 330
Eco53kI GAGCTC 4 cut(s) 2194, 3496, 4023, 5059
Eco57I CTGAAG 7 cut(s) 1515, 1783, 2609, 3639, 5225, 6030, 6059
Eco72I CACGTG 1 cut(s) 3455
Eco88I CYCGRG 1 cut(s) 4941
Eco91I GGTNACC 1 cut(s) 4000
EcoICRI GAGCTC 4 cut(s) 2194, 3496, 4023, 5059
EcoNI CCTNNNNNAGG 4 cut(s) 2243, 2623, 3212, 6048
EcoO109I RGGNCCY 4 cut(s) 1546, 3870, 5551, 5770
EcoO65I GGTNACC 1 cut(s) 4000
EcoRI GAATTC 2 cut(s) 2312, 5278
EcoRII CCWGG 6 cut(s) 888, 3600, 4096, 4466, 4656, 5068
EcoRV GATATC 1 cut(s) 5078
EcoT14I CCWWGG 2 cut(s) 2239, 4372
EcoT22I ATGCAT 1 cut(s) 2025
EcoT38I GRGCYC 6 cut(s) 2196, 3498, 3873, 4025, 4090, 5061
ErhI CCWWGG 2 cut(s) 2239, 4372
Esp3I CGTCTC 3 cut(s) 57, 5029, 5942
FalI AAGNNNNNCTT 8 cut(s) 2156, 2188, 3870, 3902, 5557, 5589, 5759, 5791
FaqI GGGAC 5 cut(s) 1559, 3095, 5223, 5738, 5756
FauI CCCGC 1 cut(s) 2649
FauNDI CATATG 2 cut(s) 2019, 3532
FblI GTMKAC 4 cut(s) 88, 1307, 1537, 3982
FriOI GRGCYC 6 cut(s) 2196, 3498, 3873, 4025, 4090, 5061
GlaI GCGC 7 cut(s) 732, 751, 2534, 2922, 2924, 3699, 3932
GsaI CCCAGC 3 cut(s) 816, 2520, 3233
GsuI CTGGAG 5 cut(s) 911, 4904, 5294, 5609, 6261
HaeII RGCGCY 1 cut(s) 3701
HgaI GACGC 6 cut(s) 668, 1724, 4239, 5227, 5727, 5924
HhaI GCGC 7 cut(s) 733, 752, 2535, 2923, 2925, 3700, 3933
Hin1I GRCGYC 3 cut(s) 610, 5738, 5935
Hin6I GCGC 7 cut(s) 731, 750, 2533, 2921, 2923, 3698, 3931
HinP1I GCGC 7 cut(s) 731, 750, 2533, 2921, 2923, 3698, 3931
HincII GTYRAC 6 cut(s) 882, 1436, 1794, 3983, 4621, 5784
HindII GTYRAC 6 cut(s) 882, 1436, 1794, 3983, 4621, 5784
HindIII AAGCTT 2 cut(s) 635, 1709
HphI GGTGA 8 cut(s) 1165, 1304, 3350, 4766, 5019, 5272, 5669, 6107
Hpy99I CGWCG 5 cut(s) 609, 612, 864, 2394, 3630
Hsp92I GRCGYC 3 cut(s) 610, 5738, 5935
HspAI GCGC 7 cut(s) 731, 750, 2533, 2921, 2923, 3698, 3931
KflI GGGWCCC 1 cut(s) 1546
Kpn2I TCCGGA 6 cut(s) 513, 1668, 1900, 2196, 4140, 5464
KpnI GGTACC 1 cut(s) 5644
MbiI CCGCTC 1 cut(s) 3658
MfeI CAATTG 5 cut(s) 875, 2419, 5103, 5929, 6216
MflI RGATCY 6 cut(s) 1081, 1583, 2959, 3803, 5302, 5703
MlsI TGGCCA 1 cut(s) 1477
MluI ACGCGT 1 cut(s) 5236
MluNI TGGCCA 1 cut(s) 1477
MlyI GAGTC 5 cut(s) 415, 3062, 3136, 5005, 5027
MmeI TCCRAC 9 cut(s) 97, 804, 2707, 2986, 3140, 3231, 3755, 3755, 5019
Mox20I TGGCCA 1 cut(s) 1477
Mph1103I ATGCAT 1 cut(s) 2025
MroI TCCGGA 6 cut(s) 513, 1668, 1900, 2196, 4140, 5464
MroXI GAANNNNTTC 3 cut(s) 636, 4853, 6072
MscI TGGCCA 1 cut(s) 1477
MslI CAYNNNNRTG 7 cut(s) 60, 162, 290, 1031, 1404, 2991, 4641
Msp20I TGGCCA 1 cut(s) 1477
MspA1I CMGCKG 6 cut(s) 1169, 2638, 2672, 3233, 4271, 4990
MspCI CTTAAG 2 cut(s) 2299, 3755
MspR9I CCNGG 9 cut(s) 890, 1392, 3602, 4098, 4468, 4658, 4688, 5070, 5750
MunI CAATTG 5 cut(s) 875, 2419, 5103, 5929, 6216
Mva1269I GAATGC 3 cut(s) 4779, 4853, 5796
MvaI CCWGG 6 cut(s) 890, 3602, 4098, 4468, 4658, 5070
MvnI CGCG 3 cut(s) 2923, 4250, 5238
NciI CCSGG 3 cut(s) 1392, 4688, 5750
NcoI CCATGG 1 cut(s) 4372
NdeI CATATG 2 cut(s) 2019, 3532
NmeAIII GCCGAG 1 cut(s) 941
NmuCI GTSAC 7 cut(s) 1171, 2135, 3909, 4215, 4251, 4273, 5375
NsiI ATGCAT 1 cut(s) 2025
NspI RCATGY 8 cut(s) 1416, 1677, 4302, 4424, 4575, 5121, 5847, 6215
NspV TTCGAA 2 cut(s) 1888, 3430
OliI CACNNNNGTG 1 cut(s) 1031
PaeI GCATGC 1 cut(s) 5121
PagI TCATGA 1 cut(s) 4873
PasI CCCWGGG 1 cut(s) 3601
PauI GCGCGC 1 cut(s) 2921
PceI AGGCCT 4 cut(s) 2888, 3098, 4889, 5906
PciI ACATGT 3 cut(s) 1673, 4420, 5843
PcsI WCGNNNNNNNCGW 2 cut(s) 601, 6055
PctI GAATGC 3 cut(s) 4779, 4853, 5796
PdmI GAANNNNTTC 3 cut(s) 636, 4853, 6072
PfeI GAWTC 6 cut(s) 473, 1427, 2594, 4112, 5087, 5167
PflFI GACNNNGTC 2 cut(s) 609, 4533
PflMI CCANNNNNTGG 2 cut(s) 2651, 4167
PfoI TCCNGGA 1 cut(s) 4096
PinAI ACCGGT 1 cut(s) 3997
PleI GAGTC 5 cut(s) 415, 3061, 3135, 5004, 5026
PmaCI CACGTG 1 cut(s) 3455
PmlI CACGTG 1 cut(s) 3455
PpsI GAGTC 5 cut(s) 415, 3061, 3135, 5004, 5026
Ppu21I YACGTR 5 cut(s) 62, 80, 3319, 3455, 4595
PpuMI RGGWCCY 3 cut(s) 1546, 5551, 5770
PscI ACATGT 3 cut(s) 1673, 4420, 5843
PshBI ATTAAT 2 cut(s) 356, 4431
PsiI TTATAA 2 cut(s) 1700, 4224
Psp124BI GAGCTC 4 cut(s) 2196, 3498, 4025, 5061
Psp5II RGGWCCY 3 cut(s) 1546, 5551, 5770
Psp6I CCWGG 6 cut(s) 888, 3600, 4096, 4466, 4656, 5068
PspCI CACGTG 1 cut(s) 3455
PspEI GGTNACC 1 cut(s) 4000
PspFI CCCAGC 3 cut(s) 812, 2516, 3229
PspGI CCWGG 6 cut(s) 888, 3600, 4096, 4466, 4656, 5068
PspOMI GGGCCC 1 cut(s) 3869
PspPPI RGGWCCY 3 cut(s) 1546, 5551, 5770
PsrI GAACNNNNNNTAC 4 cut(s) 954, 986, 1314, 1346
PstI CTGCAG 5 cut(s) 2643, 3004, 4387, 4606, 4995
PstNI CAGNNNCTG 2 cut(s) 662, 3559
PsuI RGATCY 6 cut(s) 1081, 1583, 2959, 3803, 5302, 5703
PsyI GACNNNGTC 2 cut(s) 609, 4533
PteI GCGCGC 1 cut(s) 2921
PvuII CAGCTG 5 cut(s) 1169, 2638, 2672, 3233, 4990
RsaI GTAC 9 cut(s) 1215, 1323, 1717, 1895, 2368, 2539, 4556, 5405, 5642
RsaNI GTAC 9 cut(s) 1214, 1322, 1716, 1894, 2367, 2538, 4555, 5404, 5641
RseI CAYNNNNRTG 7 cut(s) 60, 162, 290, 1031, 1404, 2991, 4641
SacI GAGCTC 4 cut(s) 2196, 3498, 4025, 5061
SalI GTCGAC 1 cut(s) 3981
ScaI AGTACT 1 cut(s) 1323
SchI GAGTC 5 cut(s) 415, 3062, 3136, 5005, 5027
ScrFI CCNGG 9 cut(s) 890, 1392, 3602, 4098, 4468, 4658, 4688, 5070, 5750
SfuI TTCGAA 2 cut(s) 1888, 3430
SinI GGWCC 8 cut(s) 1468, 1546, 2938, 3225, 3544, 4654, 5551, 5770
SmiMI CAYNNNNRTG 7 cut(s) 60, 162, 290, 1031, 1404, 2991, 4641
SmlI CTYRAG 9 cut(s) 98, 221, 2299, 3092, 3117, 3569, 3755, 4608, 4808
SmoI CTYRAG 9 cut(s) 98, 221, 2299, 3092, 3117, 3569, 3755, 4608, 4808
SnaBI TACGTA 1 cut(s) 4595
SpeI ACTAGT 1 cut(s) 2708
SphI GCATGC 1 cut(s) 5121
SseBI AGGCCT 4 cut(s) 2888, 3098, 4889, 5906
SsiI CCGC 7 cut(s) 2656, 3150, 3658, 3859, 4271, 5084, 5689
SspI AATATT 4 cut(s) 687, 3012, 3221, 4429
SstI GAGCTC 4 cut(s) 2196, 3498, 4025, 5061
StuI AGGCCT 4 cut(s) 2888, 3098, 4889, 5906
StyD4I CCNGG 9 cut(s) 888, 1390, 3600, 4096, 4466, 4656, 4686, 5068, 5748
StyI CCWWGG 2 cut(s) 2239, 4372
TatI WGTACW 4 cut(s) 1213, 1321, 2366, 5403
TauI GCSGC 1 cut(s) 3152
TfiI GAWTC 6 cut(s) 473, 1427, 2594, 4112, 5087, 5167
TseFI GTSAC 7 cut(s) 1171, 2135, 3909, 4215, 4251, 4273, 5375
Tsp45I GTSAC 7 cut(s) 1171, 2135, 3909, 4215, 4251, 4273, 5375
TspGWI ACGGA 2 cut(s) 3536, 5011
Tth111I GACNNNGTC 2 cut(s) 609, 4533
Van91I CCANNNNNTGG 2 cut(s) 2651, 4167
Vha464I CTTAAG 2 cut(s) 2299, 3755
VpaK11BI GGWCC 8 cut(s) 1468, 1546, 2938, 3225, 3544, 4654, 5551, 5770
VspI ATTAAT 2 cut(s) 356, 4431
XagI CCTNNNNNAGG 4 cut(s) 2243, 2623, 3212, 6048
XbaI TCTAGA 1 cut(s) 1351
XceI RCATGY 8 cut(s) 1416, 1677, 4302, 4424, 4575, 5121, 5847, 6215
XcmI CCANNNNNNNNNTGG 2 cut(s) 1183, 3911
XmiI GTMKAC 4 cut(s) 88, 1307, 1537, 3982
XmnI GAANNNNTTC 3 cut(s) 636, 4853, 6072
ZraI GACGTC 1 cut(s) 611
ZrmI AGTACT 1 cut(s) 1323
Zsp2I ATGCAT 1 cut(s) 2025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.