pycom05g15120

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011320
Physical Location & Seq
Forward (+)
18646184 .. 18647075
892 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGGCGGAGCAAAATGTCCAGAACGGTGGGGCCGCGGAGAAGGTCGCACCCGTGTCCCTTATCACTGTCACGGAATGCCATCCTCGTCTTTCCATCAAGGCGCCCAATGGCGAAGGCGCCATCAAGTTCTACGTGGCCGTCCTGGGCGCCGTGGAGCTTGAGCGTGAGACTGATCTCAAGCGCAAGGCTGACCAGGAGCGTCCGTACATCTACAGTGCCAAGCTCAAACTTGGGTCCATCACCTTCCTCGTGACCGAGTCTGACTCTCTTGTTGACTCCGGCGAGGGCGAAGTTGATGGTGGTTTTAAGCAAGGCTTTAACCTGTTACTTACTGTTGTGGACGTCGATGCGGCTGTGTCCAAGGCCGTCCAGGCGGGAGCTGCAGCGGTTGGGGAGATCAAAACCGGTGGTGCTTTCTTTGGCGACGAGCAGCGTTGGGCTCAGATTAAGGATCCATTCGGCATTTGCTGGTTCTTTGTCACTCCCCTTGAGAAGAAGGCCGCTGTCCCTGAAGCCCCAGCTACCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

177

Amino Acids

18.7

Weight (kDa)

4.9

Isoelectric Point (pI)

27.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 30 - 85 7.6e-17 Glyoxalase At5g48480-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48480
fragaria_vesca FvH4_2g17810
malus_domestica MD05G1159600.v1.1 MD10G1152100.v1.1
prunus_persica Prupe.8G190400_v2.0.a1
pyrus_communis pycom05g15120 pycom10g13110
rosa_chinensis RchiOBHm_Chr6g0282841
rosa_laevigata RLG00000012864 RLG00000021743
rosa_multiflora Rmu_sc0006070.1_g000011
rosa_roxburghii Rroxscaffold_7G00185960
rosa_rugosa Rorug02G0530700 Rorug06G0149900
rosa_samantha Rh2BG610300 Rh6AG263200 Rh6BG266100 Rh6CG265300 Rh6DG257900
rosa_wichuraiana Rw6G022720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
AccB1I GGYRCC 3 cut(s) 100, 116, 146
AccII CGCG 1 cut(s) 35
AciI CCGC 7 cut(s) 5, 33, 35, 350, 374, 386, 501
AclWI GGATC 2 cut(s) 446, 459
AcoI YGGCCR 1 cut(s) 135
AcuI CTGAAG 1 cut(s) 531
AcyI GRCGYC 4 cut(s) 101, 117, 147, 342
AfaI GTAC 1 cut(s) 206
AgeI ACCGGT 1 cut(s) 404
AjnI CCWGG 3 cut(s) 141, 192, 369
AluBI AGCT 4 cut(s) 157, 223, 380, 521
AluI AGCT 4 cut(s) 157, 223, 380, 521
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 2 cut(s) 446, 459
AoxI GGCC 4 cut(s) 30, 135, 363, 498
ApeKI GCWGC 3 cut(s) 380, 383, 430
AsiGI ACCGGT 1 cut(s) 404
AspLEI GCGC 4 cut(s) 103, 119, 149, 183
AspS9I GGNCC 2 cut(s) 30, 234
AsuHPI GGTGA 1 cut(s) 232
AvaII GGWCC 1 cut(s) 234
BamHI GGATCC 1 cut(s) 451
BanI GGYRCC 3 cut(s) 100, 116, 146
BanII GRGCYC 1 cut(s) 442
BauI CACGAG 1 cut(s) 248
BbvI GCAGC 3 cut(s) 367, 395, 442
BccI CCATC 5 cut(s) 87, 101, 128, 245, 290
BceAI ACGGC 3 cut(s) 122, 134, 350
BciT130I CCWGG 3 cut(s) 143, 194, 371
BcoDI GTCTC 1 cut(s) 161
BfmI CTRYAG 2 cut(s) 211, 381
BfoI RGCGCY 3 cut(s) 104, 120, 150
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 6 cut(s) 33, 351, 381, 384, 431, 501
BlsI GCNGC 6 cut(s) 34, 352, 382, 385, 432, 502
Bme1390I CCNGG 3 cut(s) 143, 194, 371
Bme18I GGWCC 1 cut(s) 234
BmgT120I GGNCC 2 cut(s) 30, 234
BmiI GGNNCC 6 cut(s) 31, 102, 118, 148, 235, 453
BmrFI CCNGG 3 cut(s) 143, 194, 371
BmsI GCATC 1 cut(s) 337
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpuEI CTTGAG 3 cut(s) 161, 179, 509
BsaAI YACGTR 1 cut(s) 133
BsaHI GRCGYC 4 cut(s) 101, 117, 147, 342
BsaJI CCNNGG 4 cut(s) 33, 142, 150, 360
BsaWI WCCGGW 1 cut(s) 404
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 404
BseBI CCWGG 3 cut(s) 143, 194, 371
BseDI CCNNGG 4 cut(s) 33, 142, 150, 360
BseGI GGATG 1 cut(s) 79
BseMII CTCAG 1 cut(s) 455
BseXI GCAGC 3 cut(s) 367, 395, 442
BseYI CCCAGC 1 cut(s) 517
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 4 cut(s) 32, 137, 365, 500
BshNI GGYRCC 3 cut(s) 100, 116, 146
BshTI ACCGGT 1 cut(s) 404
BsiSI CCGG 2 cut(s) 279, 405
BslFI GGGAC 2 cut(s) 40, 491
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 2 cut(s) 40, 491
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 4 cut(s) 32, 137, 365, 500
Bsp1286I GDGCHC 1 cut(s) 442
Bsp143I GATC 3 cut(s) 172, 396, 451
BspACI CCGC 7 cut(s) 5, 33, 35, 350, 374, 386, 501
BspANI GGCC 4 cut(s) 32, 137, 365, 500
BspCNI CTCAG 1 cut(s) 454
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 6 cut(s) 31, 102, 118, 148, 235, 453
BspMAI CTGCAG 1 cut(s) 385
BspPI GGATC 2 cut(s) 446, 459
BspT107I GGYRCC 3 cut(s) 100, 116, 146
BsrFI RCCGGY 1 cut(s) 404
BssAI RCCGGY 1 cut(s) 404
BssECI CCNNGG 4 cut(s) 33, 142, 150, 360
BssMI GATC 3 cut(s) 172, 396, 451
BssNI GRCGYC 4 cut(s) 101, 117, 147, 342
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 1 cut(s) 360
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 3 cut(s) 143, 194, 371
Bst4CI ACNGT 5 cut(s) 26, 67, 215, 334, 526
BstACI GRCGYC 4 cut(s) 101, 117, 147, 342
BstBAI YACGTR 1 cut(s) 133
BstDEI CTNAG 1 cut(s) 441
BstDSI CCRYGG 2 cut(s) 33, 150
BstF5I GGATG 1 cut(s) 79
BstFNI CGCG 1 cut(s) 35
BstH2I RGCGCY 3 cut(s) 104, 120, 150
BstHHI GCGC 4 cut(s) 103, 119, 149, 183
BstKTI GATC 3 cut(s) 175, 399, 454
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 3 cut(s) 172, 396, 451
BstMWI GCNNNNNNNGC 2 cut(s) 371, 380
BstNI CCWGG 3 cut(s) 143, 194, 371
BstSCI CCNGG 3 cut(s) 141, 192, 369
BstSFI CTRYAG 2 cut(s) 211, 381
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 367, 395, 442
BstX2I RGATCY 1 cut(s) 451
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 1 cut(s) 451
BsuRI GGCC 4 cut(s) 32, 137, 365, 500
BtgI CCRYGG 2 cut(s) 33, 150
BtsCI GGATG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 63, 220
CfoI GCGC 4 cut(s) 103, 119, 149, 183
Cfr10I RCCGGY 1 cut(s) 404
Cfr13I GGNCC 2 cut(s) 30, 234
Cfr42I CCGCGG 1 cut(s) 36
CseI GACGC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 205
CspAI ACCGGT 1 cut(s) 404
CspCI CAANNNNNGTGG 2 cut(s) 388, 423
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 1 cut(s) 441
DinI GGCGCC 3 cut(s) 102, 118, 148
DpnI GATC 3 cut(s) 174, 398, 453
DpnII GATC 3 cut(s) 172, 396, 451
EaeI YGGCCR 1 cut(s) 135
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 234
Eco57I CTGAAG 1 cut(s) 531
EcoRII CCWGG 3 cut(s) 141, 192, 369
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 442
EgeI GGCGCC 3 cut(s) 102, 118, 148
EheI GGCGCC 3 cut(s) 102, 118, 148
ErhI CCWWGG 1 cut(s) 360
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 2 cut(s) 40, 491
FauI CCCGC 1 cut(s) 367
Fnu4HI GCNGC 6 cut(s) 33, 351, 381, 384, 431, 501
FokI GGATG 1 cut(s) 66
FriOI GRGCYC 1 cut(s) 442
Fsp4HI GCNGC 6 cut(s) 33, 351, 381, 384, 431, 501
GlaI GCGC 4 cut(s) 102, 118, 148, 182
GluI GCNGC 6 cut(s) 33, 351, 381, 384, 431, 501
GsaI CCCAGC 1 cut(s) 521
HaeII RGCGCY 3 cut(s) 104, 120, 150
HaeIII GGCC 4 cut(s) 32, 137, 365, 500
HapII CCGG 2 cut(s) 279, 405
HgaI GACGC 1 cut(s) 188
HhaI GCGC 4 cut(s) 103, 119, 149, 183
Hin1I GRCGYC 4 cut(s) 101, 117, 147, 342
Hin6I GCGC 4 cut(s) 101, 117, 147, 181
HinP1I GCGC 4 cut(s) 101, 117, 147, 181
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 3 cut(s) 257, 263, 275
HpaII CCGG 2 cut(s) 279, 405
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 2 cut(s) 274, 340
Hpy188I TCNGA 2 cut(s) 262, 444
Hpy188III TCNNGA 2 cut(s) 19, 250
Hpy8I GTNNAC 2 cut(s) 274, 340
Hpy99I CGWCG 2 cut(s) 347, 428
HpyAV CCTTC 4 cut(s) 34, 107, 253, 490
HpyCH4III ACNGT 5 cut(s) 26, 67, 215, 334, 526
HpyCH4IV ACGT 2 cut(s) 132, 342
HpyCH4V TGCA 1 cut(s) 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 380
HpyF3I CTNAG 1 cut(s) 441
HpySE526I ACGT 2 cut(s) 132, 342
Hsp92I GRCGYC 4 cut(s) 101, 117, 147, 342
HspAI GCGC 4 cut(s) 101, 117, 147, 181
KasI GGCGCC 3 cut(s) 100, 116, 146
KspI CCGCGG 1 cut(s) 36
Kzo9I GATC 3 cut(s) 172, 396, 451
LmnI GCTCC 4 cut(s) 7, 154, 196, 377
Lsp1109I GCAGC 3 cut(s) 367, 395, 442
LweI GCATC 1 cut(s) 337
MaeII ACGT 2 cut(s) 132, 342
MaeIII GTNAC 4 cut(s) 67, 250, 324, 478
MalI GATC 3 cut(s) 174, 398, 453
MboI GATC 3 cut(s) 172, 396, 451
MboII GAAGA 1 cut(s) 505
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 1 cut(s) 442
Mly113I GGCGCC 3 cut(s) 101, 117, 147
MlyI GAGTC 3 cut(s) 257, 266, 269
MnlI CCTC 3 cut(s) 93, 257, 277
MseI TTAA 3 cut(s) 306, 318, 447
MspA1I CMGCKG 3 cut(s) 35, 386, 503
MspI CCGG 2 cut(s) 279, 405
MspR9I CCNGG 3 cut(s) 143, 194, 371
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 3 cut(s) 143, 194, 371
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 371, 380
NarI GGCGCC 3 cut(s) 101, 117, 147
NdeII GATC 3 cut(s) 172, 396, 451
NlaIV GGNNCC 6 cut(s) 31, 102, 118, 148, 235, 453
NmuCI GTSAC 3 cut(s) 67, 250, 478
PctI GAATGC 1 cut(s) 80
PflFI GACNNNGTC 1 cut(s) 256
PinAI ACCGGT 1 cut(s) 404
PkrI GCNGC 6 cut(s) 34, 352, 382, 385, 432, 502
PleI GAGTC 3 cut(s) 257, 265, 269
PluTI GGCGCC 3 cut(s) 104, 120, 150
PpsI GAGTC 3 cut(s) 257, 265, 269
Ppu21I YACGTR 1 cut(s) 133
Psp6I CCWGG 3 cut(s) 141, 192, 369
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 3 cut(s) 141, 192, 369
PspN4I GGNNCC 6 cut(s) 31, 102, 118, 148, 235, 453
PspPI GGNCC 2 cut(s) 30, 234
PstI CTGCAG 1 cut(s) 385
PsuI RGATCY 1 cut(s) 451
PsyI GACNNNGTC 1 cut(s) 256
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SacII CCGCGG 1 cut(s) 36
SaqAI TTAA 3 cut(s) 306, 318, 447
SatI GCNGC 6 cut(s) 33, 351, 381, 384, 431, 501
Sau3AI GATC 3 cut(s) 172, 396, 451
Sau96I GGNCC 2 cut(s) 30, 234
SchI GAGTC 3 cut(s) 257, 266, 269
ScrFI CCNGG 3 cut(s) 143, 194, 371
SduI GDGCHC 1 cut(s) 442
SetI ASST 9 cut(s) 45, 135, 159, 225, 245, 324, 345, 382, 523
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 2 cut(s) 211, 381
SfoI GGCGCC 3 cut(s) 102, 118, 148
Sfr303I CCGCGG 1 cut(s) 36
SgrBI CCGCGG 1 cut(s) 36
SinI GGWCC 1 cut(s) 234
SmlI CTYRAG 3 cut(s) 158, 176, 488
SmoI CTYRAG 3 cut(s) 158, 176, 488
SsiI CCGC 7 cut(s) 5, 33, 35, 350, 374, 386, 501
SspDI GGCGCC 3 cut(s) 100, 116, 146
StyD4I CCNGG 3 cut(s) 141, 192, 369
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 5 cut(s) 26, 67, 215, 334, 526
TaiI ACGT 2 cut(s) 135, 345
TaqI TCGA 1 cut(s) 345
TaqII GACCGA 1 cut(s) 269
TauI GCSGC 3 cut(s) 35, 353, 503
Tru1I TTAA 3 cut(s) 306, 318, 447
Tru9I TTAA 3 cut(s) 306, 318, 447
TscAI CASTG 2 cut(s) 70, 220
TseFI GTSAC 3 cut(s) 67, 250, 478
TseI GCWGC 3 cut(s) 380, 383, 430
Tsp45I GTSAC 3 cut(s) 67, 250, 478
TspGWI ACGGA 2 cut(s) 86, 192
TspRI CASTG 2 cut(s) 70, 220
Tth111I GACNNNGTC 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 234
ZraI GACGTC 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.