pycom05g15370

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013247
Physical Location & Seq
Forward (+)
18897750 .. 18900795
3046 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15370.5

Sequence Viewer

Length: 1128 bp
ATGATTAGTTTATTACTTAAACTTACAAATTTTATTTTAAAATTTAATCTTTTTAACATTATTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTGGGAAACGTACTCCCCTTTGAGGGTTTGCCTTTGCCTCTCTCTTCGTCTTCGTCTCCTCCATTTCGCGCTTTCCTTTTTATTTCCCTCACCCTTTCGCTTTCGCCCACCACCTCCTACTCCTCCTCTCTCCACCGCTTAAACATCGATTTTTGGACGCCCGCATCCGCAGAAAACGCCATAGGAAACAATCAATTTGGGGAGAGGAAGCAGAAGACGGATATGGCGCCGAGTCCCGCATCGTCGACGGCGGAGAAACTCAAGAAGGACGGCAACACCTACTTCAAGAAAGAGCGATTCGCGGCCGCCATCGATGCGTATACTGAGGCAATCACTCTGTGCCCTAATGTTCCTGTTTATTTTACGAATCGCGCTCTGTGCCATCTCAAGCGTAATGATTGGACGAAAGTCGAAGAGGATTCTCGGAGAGCTATTCAGCTTGATCATAACTCAGTTAAGGGTAACTATATATTGGGGCTTGCTTTACTACAGAAGCAAGAGTATGGCGAGGGAGTTAAGGCACTGGAGAGGGCTTTGGATCTTGGGAGGGGTGCCAATCTGAATGATTATATGGTAGAGGAGATCTGGCAGGAGCTTTCAAAAGCAAAGTACATGGAGTGGGAGGAGACATCGAGCAAACGTTCATGGGAATTGCAAAATTTGAAGTATGTCTTCCTTGGGTTCAGAGAAGCTTGTGAGACTGCTCTCAAAAAGAAACATGACTCTTGTCAAAAGAAACAGTTAGAGGCGTTAGGAAGAGTGTTCGAGAAAGCTGCAGAAGCTGACACGCCTAGTGAGGTGCCGGATTACCTATGTTGCAAAATCATGCTTGACATATTTCGTGATCCTGTGATTACTCCAAGTGGGGCTACATATGAGAGATCAGTGATCCTTCACCATCTTGAGAAGGTTGGCAATTTCGATCCAATCACTCGAGAGCCGCTTGATCAATCAGAGCTAATATCAAACTTTGCCATAAAAGAAGCAGTTCAAGCATATTTACAAGAGCATGGTTGGGCCTACAGAGCATAG

Protein Analysis

376

Amino Acids

42.7

Weight (kDa)

8.96

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07370
fragaria_vesca FvH4_2g18060 FvH4_2g18060
malus_domestica MD05G1165100.v1.1 MD10G1154400.v1.1
prunus_persica Prupe.8G193300_v2.0.a1
pyrus_communis pycom05g15370 pycom10g13350
rosa_chinensis RchiOBHm_Chr6g0283451
rosa_laevigata RLG00000012825
rosa_multiflora Rmu_sc0004959.1_g000017
rosa_roxburghii Rroxscaffold_7G00185530
rosa_rugosa Rorug06G0154300
rosa_samantha Rh6AG266600 Rh6BG269100 Rh6CG269000 Rh6DG262400
rosa_wichuraiana Rw6G023080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 322, 647, 895
AccI GTMKAC 2 cut(s) 341, 416
AccII CGCG 3 cut(s) 165, 398, 468
AciI CCGC 8 cut(s) 232, 258, 264, 333, 347, 398, 402, 1037
AclI AACGTT 1 cut(s) 736
AclWI GGATC 4 cut(s) 642, 935, 979, 1013
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 3 cut(s) 28, 41, 754
AcyI GRCGYC 2 cut(s) 254, 323
AdeI CACNNNGTG 1 cut(s) 435
AfaI GTAC 2 cut(s) 108, 707
AfiI CCNNNNNNNGG 2 cut(s) 118, 119
AgsI TTSAA 4 cut(s) 382, 696, 760, 1088
AluBI AGCT 7 cut(s) 527, 535, 691, 788, 869, 878, 1054
AluI AGCT 7 cut(s) 527, 535, 691, 788, 869, 878, 1054
Alw26I GTCTC 3 cut(s) 156, 716, 788
AlwI GGATC 4 cut(s) 642, 935, 979, 1013
AlwNI CAGNNNCTG 1 cut(s) 878
Ama87I CYCGRG 1 cut(s) 1029
AoxI GGCC 2 cut(s) 399, 1113
ApeKI GCWGC 1 cut(s) 869
ApoI RAATTY 3 cut(s) 28, 41, 754
Asp700I GAANNNNTTC 1 cut(s) 1083
AspLEI GCGC 3 cut(s) 167, 325, 470
AspS9I GGNCC 1 cut(s) 1113
AsuHPI GGTGA 2 cut(s) 178, 983
AvaI CYCGRG 1 cut(s) 1029
BaeGI GKGCMC 1 cut(s) 440
BanI GGYRCC 3 cut(s) 322, 647, 895
BbsI GAAGAC 3 cut(s) 138, 317, 760
BbvI GCAGC 1 cut(s) 856
BccI CCATC 3 cut(s) 413, 486, 1002
BceAI ACGGC 2 cut(s) 360, 382
BcgI CGANNNNNNTGC 2 cut(s) 851, 885
BclI TGATCA 2 cut(s) 538, 1042
BcoDI GTCTC 3 cut(s) 156, 716, 788
BfaI CTAG 1 cut(s) 888
BfmI CTRYAG 3 cut(s) 584, 870, 1117
BfoI RGCGCY 1 cut(s) 326
BglII AGATCT 1 cut(s) 678
BisI GCNGC 4 cut(s) 399, 402, 870, 1037
BlsI GCNGC 4 cut(s) 400, 403, 871, 1038
BmeT110I CYCGRG 1 cut(s) 1029
BmgT120I GGNCC 1 cut(s) 1113
BmiI GGNNCC 3 cut(s) 324, 649, 897
BmsI GCATC 3 cut(s) 269, 344, 400
BoxI GACNNNNGTC 2 cut(s) 503, 822
BpiI GAAGAC 3 cut(s) 138, 317, 760
BpmI CTGGAG 1 cut(s) 641
BpuEI CTTGAG 3 cut(s) 341, 467, 1019
Bsa29I ATCGAT 2 cut(s) 243, 408
BsaHI GRCGYC 2 cut(s) 254, 323
BsaJI CCNNGG 1 cut(s) 772
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 119
Bse1I ACTGG 1 cut(s) 624
BseCI ATCGAT 2 cut(s) 243, 408
BseDI CCNNGG 1 cut(s) 772
BseGI GGATG 1 cut(s) 260
BseLI CCNNNNNNNGG 2 cut(s) 118, 119
BseMII CTCAG 2 cut(s) 411, 561
BseNI ACTGG 1 cut(s) 624
BseRI GAGGAG 5 cut(s) 144, 208, 211, 689, 734
BseSI GKGCMC 1 cut(s) 440
BseX3I CGGCCG 1 cut(s) 399
BseXI GCAGC 1 cut(s) 856
Bsh1236I CGCG 3 cut(s) 165, 398, 468
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 2 cut(s) 401, 1115
BshNI GGYRCC 3 cut(s) 322, 647, 895
BshVI ATCGAT 2 cut(s) 243, 408
BsiEI CGRYCG 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 1 cut(s) 899
BslFI GGGAC 1 cut(s) 315
BslI CCNNNNNNNGG 2 cut(s) 118, 119
BsmAI GTCTC 3 cut(s) 156, 716, 788
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 315
BsnI GGCC 2 cut(s) 401, 1115
BsoBI CYCGRG 1 cut(s) 1029
Bsp1286I GDGCHC 1 cut(s) 440
Bsp143I GATC 8 cut(s) 538, 634, 678, 940, 977, 984, 1018, 1042
BspACI CCGC 8 cut(s) 232, 258, 264, 333, 347, 398, 402, 1037
BspANI GGCC 2 cut(s) 401, 1115
BspCNI CTCAG 2 cut(s) 412, 560
BspDI ATCGAT 2 cut(s) 243, 408
BspFNI CGCG 3 cut(s) 165, 398, 468
BspLI GGNNCC 3 cut(s) 324, 649, 897
BspMAI CTGCAG 1 cut(s) 874
BspPI GGATC 4 cut(s) 642, 935, 979, 1013
BspT107I GGYRCC 3 cut(s) 322, 647, 895
BsrI ACTGG 1 cut(s) 624
BssECI CCNNGG 1 cut(s) 772
BssMI GATC 8 cut(s) 538, 634, 678, 940, 977, 984, 1018, 1042
BssNAI GTATAC 1 cut(s) 417
BssNI GRCGYC 2 cut(s) 254, 323
BssT1I CCWWGG 1 cut(s) 772
Bst1107I GTATAC 1 cut(s) 417
Bst4CI ACNGT 1 cut(s) 837
Bst6I CTCTTC 3 cut(s) 145, 504, 847
BstACI GRCGYC 2 cut(s) 254, 323
BstC8I GCNNGC 2 cut(s) 258, 576
BstDEI CTNAG 2 cut(s) 420, 547
BstF5I GGATG 1 cut(s) 260
BstFNI CGCG 3 cut(s) 165, 398, 468
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 3 cut(s) 167, 325, 470
BstKTI GATC 8 cut(s) 541, 637, 681, 943, 980, 987, 1021, 1045
BstMAI GTCTC 3 cut(s) 156, 716, 788
BstMBI GATC 8 cut(s) 538, 634, 678, 940, 977, 984, 1018, 1042
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 6 cut(s) 272, 410, 474, 875, 1088, 1121
BstPAI GACNNNNGTC 2 cut(s) 503, 822
BstSFI CTRYAG 3 cut(s) 584, 870, 1117
BstSLI GKGCMC 1 cut(s) 440
BstUI CGCG 3 cut(s) 165, 398, 468
BstV1I GCAGC 1 cut(s) 856
BstV2I GAAGAC 3 cut(s) 138, 317, 760
BstX2I RGATCY 2 cut(s) 634, 678
BstYI RGATCY 2 cut(s) 634, 678
BstZ17I GTATAC 1 cut(s) 417
BstZI CGGCCG 1 cut(s) 399
Bsu15I ATCGAT 2 cut(s) 243, 408
BsuRI GGCC 2 cut(s) 401, 1115
BsuTUI ATCGAT 2 cut(s) 243, 408
BtsCI GGATG 1 cut(s) 260
BtsIMutI CAGTG 2 cut(s) 617, 987
Cac8I GCNNGC 2 cut(s) 258, 576
CaiI CAGNNNCTG 1 cut(s) 878
CciNI GCGGCCGC 1 cut(s) 399
CfoI GCGC 3 cut(s) 167, 325, 470
Cfr13I GGNCC 1 cut(s) 1113
ClaI ATCGAT 2 cut(s) 243, 408
CseI GACGC 1 cut(s) 262
Csp6I GTAC 2 cut(s) 107, 706
CviAII CATG 5 cut(s) 709, 741, 815, 922, 1106
CviQI GTAC 2 cut(s) 107, 706
DdeI CTNAG 2 cut(s) 420, 547
DinI GGCGCC 1 cut(s) 324
DpnI GATC 8 cut(s) 540, 636, 680, 942, 979, 986, 1020, 1044
DpnII GATC 8 cut(s) 538, 634, 678, 940, 977, 984, 1018, 1042
DraI TTTAAA 1 cut(s) 39
DraIII CACNNNGTG 1 cut(s) 435
EaeI YGGCCR 1 cut(s) 399
EagI CGGCCG 1 cut(s) 399
Eam1104I CTCTTC 3 cut(s) 145, 504, 847
EarI CTCTTC 3 cut(s) 145, 504, 847
EciI GGCGGA 1 cut(s) 362
EclXI CGGCCG 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 772
Eco52I CGGCCG 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 1029
EcoT14I CCWWGG 1 cut(s) 772
EgeI GGCGCC 1 cut(s) 324
EheI GGCGCC 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 772
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 5 cut(s) 712, 744, 818, 925, 1109
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FaqI GGGAC 1 cut(s) 315
FatI CATG 5 cut(s) 708, 740, 814, 921, 1105
FauI CCCGC 2 cut(s) 265, 340
FauNDI CATATG 1 cut(s) 970
FbaI TGATCA 2 cut(s) 538, 1042
FblI GTMKAC 2 cut(s) 341, 416
Fnu4HI GCNGC 4 cut(s) 399, 402, 870, 1037
FokI GGATG 1 cut(s) 247
Fsp4HI GCNGC 4 cut(s) 399, 402, 870, 1037
FspBI CTAG 1 cut(s) 888
GlaI GCGC 3 cut(s) 166, 324, 469
GluI GCNGC 4 cut(s) 399, 402, 870, 1037
GsuI CTGGAG 1 cut(s) 641
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 2 cut(s) 401, 1115
HapII CCGG 1 cut(s) 899
HgaI GACGC 1 cut(s) 262
HhaI GCGC 3 cut(s) 167, 325, 470
Hin1I GRCGYC 2 cut(s) 254, 323
Hin1II CATG 5 cut(s) 712, 744, 818, 925, 1109
Hin6I GCGC 3 cut(s) 165, 323, 468
HinP1I GCGC 3 cut(s) 165, 323, 468
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 786
HinfI GANTC 5 cut(s) 328, 393, 463, 515, 818
HpaII CCGG 1 cut(s) 899
HphI GGTGA 2 cut(s) 178, 983
Hpy166II GTNNAC 2 cut(s) 342, 417
Hpy188I TCNGA 4 cut(s) 522, 657, 782, 1051
Hpy188III TCNNGA 6 cut(s) 358, 382, 862, 938, 998, 1031
Hpy8I GTNNAC 2 cut(s) 342, 417
Hpy99I CGWCG 2 cut(s) 343, 346
HpyAV CCTTC 3 cut(s) 355, 997, 998
HpyCH4III ACNGT 1 cut(s) 837
HpyCH4IV ACGT 2 cut(s) 105, 736
HpyCH4V TGCA 3 cut(s) 751, 872, 915
HpyF10VI GCNNNNNNNGC 6 cut(s) 272, 410, 474, 875, 1088, 1121
HpyF3I CTNAG 2 cut(s) 420, 547
HpySE526I ACGT 2 cut(s) 105, 736
Hsp92I GRCGYC 2 cut(s) 254, 323
Hsp92II CATG 5 cut(s) 712, 744, 818, 925, 1109
HspAI GCGC 3 cut(s) 165, 323, 468
KasI GGCGCC 1 cut(s) 322
Ksp22I TGATCA 2 cut(s) 538, 1042
Kzo9I GATC 8 cut(s) 538, 634, 678, 940, 977, 984, 1018, 1042
LmnI GCTCC 1 cut(s) 688
LpnPI CCDG 6 cut(s) 462, 605, 667, 671, 912, 957
Lsp1109I GCAGC 1 cut(s) 856
LweI GCATC 3 cut(s) 269, 344, 400
MaeI CTAG 1 cut(s) 888
MaeII ACGT 2 cut(s) 105, 736
MaeIII GTNAC 1 cut(s) 557
MalI GATC 8 cut(s) 540, 636, 680, 942, 979, 986, 1020, 1044
MboI GATC 8 cut(s) 538, 634, 678, 940, 977, 984, 1018, 1042
MboII GAAGA 6 cut(s) 132, 138, 322, 521, 760, 864
MflI RGATCY 2 cut(s) 634, 678
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 7 cut(s) 28, 41, 95, 290, 746, 754, 1012
Mly113I GGCGCC 1 cut(s) 323
MlyI GAGTC 2 cut(s) 337, 812
MroXI GAANNNNTTC 1 cut(s) 1083
MseI TTAA 8 cut(s) 18, 38, 45, 54, 66, 236, 552, 612
MspI CCGG 1 cut(s) 899
MvnI CGCG 3 cut(s) 165, 398, 468
MwoI GCNNNNNNNGC 6 cut(s) 272, 410, 474, 875, 1088, 1121
NarI GGCGCC 1 cut(s) 323
NdeI CATATG 1 cut(s) 970
NdeII GATC 8 cut(s) 538, 634, 678, 940, 977, 984, 1018, 1042
NlaIII CATG 5 cut(s) 712, 744, 818, 925, 1109
NlaIV GGNNCC 3 cut(s) 324, 649, 897
NmeAIII GCCGAG 1 cut(s) 351
NotI GCGGCCGC 1 cut(s) 399
PaeR7I CTCGAG 1 cut(s) 1029
PdmI GAANNNNTTC 1 cut(s) 1083
PfeI GAWTC 3 cut(s) 393, 463, 515
PkrI GCNGC 4 cut(s) 400, 403, 871, 1038
PleI GAGTC 2 cut(s) 336, 812
PluTI GGCGCC 1 cut(s) 326
PpsI GAGTC 2 cut(s) 336, 812
PshAI GACNNNNGTC 2 cut(s) 503, 822
Psp1406I AACGTT 1 cut(s) 736
PspN4I GGNNCC 3 cut(s) 324, 649, 897
PspPI GGNCC 1 cut(s) 1113
PstI CTGCAG 1 cut(s) 874
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 2 cut(s) 634, 678
RsaI GTAC 2 cut(s) 108, 707
RsaNI GTAC 2 cut(s) 107, 706
SalI GTCGAC 1 cut(s) 340
SaqAI TTAA 8 cut(s) 18, 38, 45, 54, 66, 236, 552, 612
SatI GCNGC 4 cut(s) 399, 402, 870, 1037
Sau3AI GATC 8 cut(s) 538, 634, 678, 940, 977, 984, 1018, 1042
Sau96I GGNCC 1 cut(s) 1113
SchI GAGTC 2 cut(s) 337, 812
SduI GDGCHC 1 cut(s) 440
SfaNI GCATC 3 cut(s) 269, 344, 400
SfcI CTRYAG 3 cut(s) 584, 870, 1117
SfoI GGCGCC 1 cut(s) 324
Sfr274I CTCGAG 1 cut(s) 1029
SgrDI CGTCGACG 1 cut(s) 340
SlaI CTCGAG 1 cut(s) 1029
SmlI CTYRAG 4 cut(s) 356, 482, 998, 1029
SmoI CTYRAG 4 cut(s) 356, 482, 998, 1029
Sse9I AATT 7 cut(s) 28, 41, 95, 290, 746, 754, 1012
SsiI CCGC 8 cut(s) 232, 258, 264, 333, 347, 398, 402, 1037
SspDI GGCGCC 1 cut(s) 322
SspMI CTAG 1 cut(s) 888
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 1 cut(s) 837
TaiI ACGT 2 cut(s) 108, 739
TaqI TCGA 8 cut(s) 243, 341, 408, 507, 728, 861, 1017, 1030
TasI AATT 7 cut(s) 28, 41, 95, 290, 746, 754, 1012
TatI WGTACW 1 cut(s) 705
TauI GCSGC 3 cut(s) 401, 404, 1039
TfiI GAWTC 3 cut(s) 393, 463, 515
Tru1I TTAA 8 cut(s) 18, 38, 45, 54, 66, 236, 552, 612
Tru9I TTAA 8 cut(s) 18, 38, 45, 54, 66, 236, 552, 612
TscAI CASTG 2 cut(s) 624, 987
TseI GCWGC 1 cut(s) 869
TspDTI ATGAA 1 cut(s) 729
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 2 cut(s) 624, 987
XapI RAATTY 3 cut(s) 28, 41, 754
XhoI CTCGAG 1 cut(s) 1029
XmiI GTMKAC 2 cut(s) 341, 416
XmnI GAANNNNTTC 1 cut(s) 1083
XspI CTAG 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.