Rorug06G0154300

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013247
Physical Location & Seq
Forward (+)
22135009 .. 22137391
2383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0154300.1

Sequence Viewer

Length: 1785 bp
ATGGTTCGGCCAATCTTATACAAAGTGGATCCATCGGACGTAAGACACCAAACAGGCAAATTTGGTGAGGCAGTTGCAGAGCACGAACGCAGATTCAAGGATGACATGGACAAGGTGTTAAGATGGAGAGCATCTCTTTCAGAAGCAGCAAATTTATCTGGGTTGCGTTTCTCGGGCACCGGGTATGAATATGCATTTATTGATAAAATTGTTGAAGAGATTACCGCACAAGTAAAAGAACCTATCTGTTTGGATATGCCAAAGTGCCAAGTTGGGCTAGACTCTCAGGTAGGACGTATACTTAAAATGTTAGATGTTGGCGGAAGTGATGTACGCATGGTAGGAATATGGGGAATTGGCGGAATAGGGAAGACAACAATTGCTAAAGCTGTTCACAATACAATTGCCCATAAGTTTGATGGTAGCTGCTTTTTGGCAAATGTTAGAGAAGGTCAGAGCAACATGGAGAAATTAGCTGGGAGAACTGATTGGTTTGGTCACGGCAGCAGAGTTATCATAACAACAAGAGATAAGCATTTGTTGATTGCTCATAAAGTGAAACCAATATACAAGGTTCATGAACTAGGTCATCATGAAGCTCTTGAGCTCTTCAGTTCAAATGCATTCAAGGATAATAAGAATTTAGATGATAATGAGAAATTGTCAGTTACTACTGTTGTTAAATATGCTCAAGGCATTCCACTTGCACTGGAAGTTTTAGGTTCATATCTATGTGATACCTCTATACATAAATGGCAAGCTATGTTAGATGGTTTTAAAAAGAATCCTCCTAGGCGCATTCAAGATATTCTCATAATCAGTTATGATGGACTGCAAGATACAGTGCAAAACGTTTTCCTTGACATTGCATGTTTTTTCAAATGTTGGAAGAGAAATGAAATGATACAAATACTTGAAGGTTGTGACCGCATCAACCCTGAGCATAGTATTAAAGTTCTCGAAGAAAAGGCTCTCATATATGTTGGTGGAGATGGTCAGATTTGCATGCATGATTTGCTGGAAGGGATGGGAAAAGATAAAGTTTTGCAAGAGTCTACAGAGCCCGGTGAGCGAAGCAGATTGTGGCGTCACAAGGATGTGCAAGAGGTTCTAACAGAAAACACGGGAACAAGCAAAATTGAAGGCATGATGGTAAAGATGCCCAAAGAAGATGAGATACGCTTGAGTCCTAAATGCTTCAAAAAGATGAGAAATCTTAAAATTTTTATAAACGTCAATGGACAGATTTGTGGAAAGGTTGATTATTATCCGAATCAGTTGCGGTTACTTGATTGGCCTGACTGTCCACTGAAATCTTTGCCCTCCAATTTTAATACGAAGAATCTAGTTCGACTTAGTATGCCTTGCAGCCGCATCTCACGTTTTGGAGAAGGATTTAAGAGTATGGAAAATCTGAAATCCTTAAATTTGAAGAGATGTGAATTCCTAGCATCAAGCCCAGACCTGTCTGGAAGCCCAAACTTAGAGTTTCTGGATCTAAGTGGGTGTAAAAGGTTAGAGGAGGTTCACCCTTCGGTTGGATCCCTCAAAAAGCTTGTTCACCTCTATCTTCAAGGATGCTCTAAACTTCGGATGCTACCTGGCGAAGTCAACTGGAGATCCCTCCAATACATTAATCTTGACCATTGCAGAATGCTGGAGAGTTTTCCTGAAATTGTGGGAGAGATGAAATACATGACATCCTTGCGTCTGTGCGACACTGGCATCAAAGCTTTGCCTTCATCCAAAGAGAAAAGAAAAGAGAAGTGTAGGAGAAAACAATGA

Protein Analysis

594

Amino Acids

67.52

Weight (kDa)

8.86

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 3 - 78 2.9e-13 TIR domain
NB-ARC PF00931 95 - 145 3.5e-06 NB-ARC domain
WHD_ROQ1 PF23282 283 - 350 2.2e-09 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07370
fragaria_vesca FvH4_2g18060 FvH4_2g18060
malus_domestica MD05G1165100.v1.1 MD10G1154400.v1.1
prunus_persica Prupe.8G193300_v2.0.a1
pyrus_communis pycom05g15370 pycom10g13350
rosa_chinensis RchiOBHm_Chr6g0283451
rosa_laevigata RLG00000012825
rosa_multiflora Rmu_sc0004959.1_g000017
rosa_roxburghii Rroxscaffold_7G00185530
rosa_rugosa Rorug06G0154300
rosa_samantha Rh6AG266600 Rh6BG269100 Rh6CG269000 Rh6DG262400
rosa_wichuraiana Rw6G023080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1229
AccB1I GGYRCC 1 cut(s) 176
AccI GTMKAC 2 cut(s) 298, 1055
AciI CCGC 6 cut(s) 225, 321, 360, 928, 1282, 1372
AclI AACGTT 1 cut(s) 852
AclWI GGATC 6 cut(s) 23, 36, 1503, 1536, 1549, 1614
AcoI YGGCCR 1 cut(s) 8
AcsI RAATTY 6 cut(s) 59, 151, 640, 1221, 1426, 1442
AcuI CTGAAG 1 cut(s) 595
AcyI GRCGYC 1 cut(s) 1087
AfaI GTAC 1 cut(s) 333
AfiI CCNNNNNNNGG 1 cut(s) 1538
AjnI CCWGG 1 cut(s) 1600
AluBI AGCT 8 cut(s) 389, 426, 476, 599, 607, 761, 1555, 1733
AluI AGCT 8 cut(s) 389, 426, 476, 599, 607, 761, 1555, 1733
Alw21I GWGCWC 2 cut(s) 84, 609
AlwI GGATC 6 cut(s) 23, 36, 1503, 1536, 1549, 1614
Ama87I CYCGRG 1 cut(s) 172
AoxI GGCC 2 cut(s) 8, 1295
ApeKI GCWGC 4 cut(s) 146, 426, 504, 1368
ApoI RAATTY 6 cut(s) 59, 151, 640, 1221, 1426, 1442
AseI ATTAAT 1 cut(s) 1635
AspA2I CCTAGG 1 cut(s) 791
AspLEI GCGC 1 cut(s) 798
AsuC2I CCSGG 2 cut(s) 181, 1065
AsuHPI GGTGA 4 cut(s) 77, 1079, 1520, 1553
AvaI CYCGRG 1 cut(s) 172
AvrII CCTAGG 1 cut(s) 791
BaeGI GKGCMC 1 cut(s) 179
BamHI GGATCC 2 cut(s) 28, 1541
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 2 cut(s) 609, 1065
BarI GAAGNNNNNNTAC 2 cut(s) 1161, 1193
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 2 cut(s) 84, 609
BbvI GCAGC 4 cut(s) 158, 413, 516, 1380
BccI CCATC 8 cut(s) 40, 117, 413, 764, 821, 986, 1021, 1144
BceAI ACGGC 1 cut(s) 517
BcgI CGANNNNNNTGC 2 cut(s) 1261, 1295
BciT130I CCWGG 1 cut(s) 1602
BcnI CCSGG 2 cut(s) 181, 1065
BfaI CTAG 5 cut(s) 278, 584, 792, 1346, 1448
BfmI CTRYAG 1 cut(s) 1056
BisI GCNGC 5 cut(s) 147, 427, 505, 1369, 1372
BlnI CCTAGG 1 cut(s) 791
BlsI GCNGC 5 cut(s) 148, 428, 506, 1370, 1373
Bme1390I CCNGG 3 cut(s) 181, 1065, 1602
BmeT110I CYCGRG 1 cut(s) 172
BmiI GGNNCC 3 cut(s) 30, 178, 1543
BmrFI CCNGG 3 cut(s) 181, 1065, 1602
BmsI GCATC 8 cut(s) 140, 939, 1149, 1383, 1460, 1568, 1584, 1734
BpiI GAAGAC 1 cut(s) 377
BplI GAGNNNNNCTC 2 cut(s) 118, 150
BpmI CTGGAG 2 cut(s) 1636, 1679
Bpu10I CCTNAGC 1 cut(s) 939
BpuEI CTTGAG 3 cut(s) 623, 675, 1204
BpuMI CCSGG 2 cut(s) 181, 1065
BsaBI GATNNNNATC 1 cut(s) 1266
BsaHI GRCGYC 1 cut(s) 1087
BsaJI CCNNGG 1 cut(s) 791
Bsc4I CCNNNNNNNGG 1 cut(s) 1538
Bse1I ACTGG 3 cut(s) 714, 1619, 1726
Bse3DI GCAATG 2 cut(s) 864, 1645
Bse8I GATNNNNATC 1 cut(s) 1266
BseBI CCWGG 1 cut(s) 1602
BseDI CCNNGG 1 cut(s) 791
BseGI GGATG 7 cut(s) 106, 1032, 1102, 1583, 1599, 1700, 1742
BseJI GATNNNNATC 1 cut(s) 1266
BseLI CCNNNNNNNGG 1 cut(s) 1538
BseMI GCAATG 2 cut(s) 864, 1645
BseMII CTCAG 2 cut(s) 299, 930
BseNI ACTGG 3 cut(s) 714, 1619, 1726
BseRI GAGGAG 1 cut(s) 1535
BseSI GKGCMC 1 cut(s) 179
BseXI GCAGC 4 cut(s) 158, 413, 516, 1380
BseYI CCCAGC 1 cut(s) 476
BshFI GGCC 2 cut(s) 10, 1297
BshNI GGYRCC 1 cut(s) 176
BsiHKAI GWGCWC 2 cut(s) 84, 609
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 2 cut(s) 180, 1065
BslI CCNNNNNNNGG 1 cut(s) 1538
BsmI GAATGC 4 cut(s) 623, 696, 798, 1659
BsnI GGCC 2 cut(s) 10, 1297
BsoBI CYCGRG 1 cut(s) 172
Bsp1286I GDGCHC 4 cut(s) 84, 179, 609, 1065
Bsp143I GATC 4 cut(s) 28, 1495, 1541, 1619
BspACI CCGC 6 cut(s) 225, 321, 360, 928, 1282, 1372
BspANI GGCC 2 cut(s) 10, 1297
BspCNI CTCAG 2 cut(s) 298, 931
BspHI TCATGA 2 cut(s) 577, 592
BspLI GGNNCC 3 cut(s) 30, 178, 1543
BspPI GGATC 6 cut(s) 23, 36, 1503, 1536, 1549, 1614
BspQI GCTCTTC 1 cut(s) 614
BspT107I GGYRCC 1 cut(s) 176
BsrDI GCAATG 2 cut(s) 864, 1645
BsrI ACTGG 3 cut(s) 714, 1619, 1726
BssECI CCNNGG 1 cut(s) 791
BssMI GATC 4 cut(s) 28, 1495, 1541, 1619
BssNAI GTATAC 1 cut(s) 299
BssNI GRCGYC 1 cut(s) 1087
BssT1I CCWWGG 1 cut(s) 791
Bst1107I GTATAC 1 cut(s) 299
Bst2UI CCWGG 1 cut(s) 1602
Bst4CI ACNGT 3 cut(s) 676, 844, 1304
Bst6I CTCTTC 4 cut(s) 210, 614, 884, 1427
BstACI GRCGYC 1 cut(s) 1087
BstAPI GCANNNNNTGC 1 cut(s) 1015
BstC8I GCNNGC 2 cut(s) 759, 1007
BstDEI CTNAG 5 cut(s) 285, 939, 1355, 1483, 1499
BstF5I GGATG 7 cut(s) 106, 1032, 1102, 1583, 1599, 1700, 1742
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 4 cut(s) 31, 1498, 1544, 1622
BstMBI GATC 4 cut(s) 28, 1495, 1541, 1619
BstMWI GCNNNNNNNGC 3 cut(s) 1015, 1069, 1722
BstNI CCWGG 1 cut(s) 1602
BstNSI RCATGY 2 cut(s) 873, 1009
BstSCI CCNGG 3 cut(s) 179, 1063, 1600
BstSFI CTRYAG 1 cut(s) 1056
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 4 cut(s) 158, 413, 516, 1380
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 4 cut(s) 28, 1495, 1541, 1619
BstYI RGATCY 4 cut(s) 28, 1495, 1541, 1619
BstZ17I GTATAC 1 cut(s) 299
BsuRI GGCC 2 cut(s) 10, 1297
BtsCI GGATG 7 cut(s) 106, 1032, 1102, 1583, 1599, 1700, 1742
BtsIMutI CAGTG 4 cut(s) 707, 849, 1307, 1719
Cac8I GCNNGC 2 cut(s) 759, 1007
CciI TCATGA 2 cut(s) 577, 592
CfoI GCGC 1 cut(s) 798
CseI GACGC 2 cut(s) 1076, 1697
Csp6I GTAC 1 cut(s) 332
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 5 cut(s) 285, 939, 1355, 1483, 1499
DpnI GATC 4 cut(s) 30, 1497, 1543, 1621
DpnII GATC 4 cut(s) 28, 1495, 1541, 1619
DraI TTTAAA 1 cut(s) 778
EaeI YGGCCR 1 cut(s) 8
Eam1104I CTCTTC 4 cut(s) 210, 614, 884, 1427
EarI CTCTTC 4 cut(s) 210, 614, 884, 1427
EciI GGCGGA 2 cut(s) 336, 375
Ecl136II GAGCTC 1 cut(s) 607
Eco130I CCWWGG 1 cut(s) 791
Eco24I GRGCYC 2 cut(s) 609, 1065
Eco53kI GAGCTC 1 cut(s) 607
Eco57I CTGAAG 1 cut(s) 595
Eco88I CYCGRG 1 cut(s) 172
EcoICRI GAGCTC 1 cut(s) 607
EcoRI GAATTC 1 cut(s) 1442
EcoRII CCWGG 1 cut(s) 1600
EcoT14I CCWWGG 1 cut(s) 791
EcoT22I ATGCAT 3 cut(s) 196, 625, 1011
EcoT38I GRGCYC 2 cut(s) 609, 1065
ErhI CCWWGG 1 cut(s) 791
FblI GTMKAC 2 cut(s) 298, 1055
Fnu4HI GCNGC 5 cut(s) 147, 427, 505, 1369, 1372
FokI GGATG 7 cut(s) 113, 1039, 1109, 1590, 1606, 1687, 1729
FriOI GRGCYC 2 cut(s) 609, 1065
Fsp4HI GCNGC 5 cut(s) 147, 427, 505, 1369, 1372
FspBI CTAG 5 cut(s) 278, 584, 792, 1346, 1448
GlaI GCGC 1 cut(s) 797
GluI GCNGC 5 cut(s) 147, 427, 505, 1369, 1372
GsaI CCCAGC 1 cut(s) 480
GsuI CTGGAG 2 cut(s) 1636, 1679
HaeIII GGCC 2 cut(s) 10, 1297
HapII CCGG 2 cut(s) 180, 1065
HgaI GACGC 2 cut(s) 1076, 1697
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 1 cut(s) 1087
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HincII GTYRAC 1 cut(s) 1612
HindII GTYRAC 1 cut(s) 1612
HindIII AAGCTT 2 cut(s) 1553, 1731
HinfI GANTC 7 cut(s) 93, 281, 784, 1052, 1186, 1273, 1342
HpaII CCGG 2 cut(s) 180, 1065
HphI GGTGA 4 cut(s) 77, 1079, 1520, 1553
Hpy166II GTNNAC 7 cut(s) 299, 394, 1056, 1307, 1528, 1561, 1612
Hpy188I TCNGA 7 cut(s) 37, 142, 456, 999, 1272, 1416, 1593
Hpy188III TCNNGA 9 cut(s) 578, 593, 602, 803, 959, 1470, 1493, 1640, 1670
Hpy8I GTNNAC 7 cut(s) 299, 394, 1056, 1307, 1528, 1561, 1612
HpyAV CCTTC 7 cut(s) 443, 911, 1016, 1136, 1385, 1542, 1749
HpyCH4III ACNGT 3 cut(s) 676, 844, 1304
HpyCH4IV ACGT 5 cut(s) 39, 295, 852, 1233, 1381
HpyF10VI GCNNNNNNNGC 3 cut(s) 1015, 1069, 1722
HpyF3I CTNAG 5 cut(s) 285, 939, 1355, 1483, 1499
HpySE526I ACGT 5 cut(s) 39, 295, 852, 1233, 1381
Hsp92I GRCGYC 1 cut(s) 1087
HspAI GCGC 1 cut(s) 796
Kzo9I GATC 4 cut(s) 28, 1495, 1541, 1619
LguI GCTCTTC 1 cut(s) 614
Lsp1109I GCAGC 4 cut(s) 158, 413, 516, 1380
LweI GCATC 8 cut(s) 140, 939, 1149, 1383, 1460, 1568, 1584, 1734
MaeI CTAG 5 cut(s) 278, 584, 792, 1346, 1448
MaeII ACGT 5 cut(s) 39, 295, 852, 1233, 1381
MaeIII GTNAC 5 cut(s) 497, 667, 923, 1088, 1284
MalI GATC 4 cut(s) 30, 1497, 1543, 1621
MboI GATC 4 cut(s) 28, 1495, 1541, 1619
MboII GAAGA 9 cut(s) 227, 382, 601, 901, 974, 1181, 1351, 1444, 1562
MfeI CAATTG 2 cut(s) 378, 402
MflI RGATCY 4 cut(s) 28, 1495, 1541, 1619
MhlI GDGCHC 4 cut(s) 84, 179, 609, 1065
MlyI GAGTC 3 cut(s) 275, 1061, 1195
MmeI TCCRAC 2 cut(s) 866, 1519
Mph1103I ATGCAT 3 cut(s) 196, 625, 1011
MslI CAYNNNNRTG 2 cut(s) 730, 1095
MspI CCGG 2 cut(s) 180, 1065
MspR9I CCNGG 3 cut(s) 181, 1065, 1602
MunI CAATTG 2 cut(s) 378, 402
Mva1269I GAATGC 4 cut(s) 623, 696, 798, 1659
MvaI CCWGG 1 cut(s) 1602
MwoI GCNNNNNNNGC 3 cut(s) 1015, 1069, 1722
NciI CCSGG 2 cut(s) 181, 1065
NdeII GATC 4 cut(s) 28, 1495, 1541, 1619
NlaIV GGNNCC 3 cut(s) 30, 178, 1543
NmuCI GTSAC 3 cut(s) 497, 923, 1088
NsiI ATGCAT 3 cut(s) 196, 625, 1011
NspI RCATGY 2 cut(s) 873, 1009
PaeI GCATGC 1 cut(s) 1009
PagI TCATGA 2 cut(s) 577, 592
PciSI GCTCTTC 1 cut(s) 614
PctI GAATGC 4 cut(s) 623, 696, 798, 1659
PfeI GAWTC 4 cut(s) 93, 784, 1273, 1342
PkrI GCNGC 5 cut(s) 148, 428, 506, 1370, 1373
PleI GAGTC 3 cut(s) 275, 1060, 1194
PpsI GAGTC 3 cut(s) 275, 1060, 1194
PshBI ATTAAT 1 cut(s) 1635
PsiI TTATAA 1 cut(s) 1229
Psp124BI GAGCTC 1 cut(s) 609
Psp1406I AACGTT 1 cut(s) 852
Psp6I CCWGG 1 cut(s) 1600
PspFI CCCAGC 1 cut(s) 476
PspGI CCWGG 1 cut(s) 1600
PspN4I GGNNCC 3 cut(s) 30, 178, 1543
PsuI RGATCY 4 cut(s) 28, 1495, 1541, 1619
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
RseI CAYNNNNRTG 2 cut(s) 730, 1095
SacI GAGCTC 1 cut(s) 609
SapI GCTCTTC 1 cut(s) 614
SatI GCNGC 5 cut(s) 147, 427, 505, 1369, 1372
Sau3AI GATC 4 cut(s) 28, 1495, 1541, 1619
SchI GAGTC 3 cut(s) 275, 1061, 1195
ScrFI CCNGG 3 cut(s) 181, 1065, 1602
SduI GDGCHC 4 cut(s) 84, 179, 609, 1065
SfaNI GCATC 8 cut(s) 140, 939, 1149, 1383, 1460, 1568, 1584, 1734
SfcI CTRYAG 1 cut(s) 1056
SmiMI CAYNNNNRTG 2 cut(s) 730, 1095
SmlI CTYRAG 3 cut(s) 602, 690, 1183
SmoI CTYRAG 3 cut(s) 602, 690, 1183
SphI GCATGC 1 cut(s) 1009
SsiI CCGC 6 cut(s) 225, 321, 360, 928, 1282, 1372
SspMI CTAG 5 cut(s) 278, 584, 792, 1346, 1448
SstI GAGCTC 1 cut(s) 609
StyD4I CCNGG 3 cut(s) 179, 1063, 1600
StyI CCWWGG 1 cut(s) 791
TaaI ACNGT 3 cut(s) 676, 844, 1304
TaiI ACGT 5 cut(s) 42, 298, 855, 1236, 1384
TaqI TCGA 2 cut(s) 960, 1351
TauI GCSGC 1 cut(s) 1374
TfiI GAWTC 4 cut(s) 93, 784, 1273, 1342
TscAI CASTG 4 cut(s) 714, 849, 1314, 1726
TseFI GTSAC 3 cut(s) 497, 923, 1088
TseI GCWGC 4 cut(s) 146, 426, 504, 1368
Tsp45I GTSAC 3 cut(s) 497, 923, 1088
TspDTI ATGAA 8 cut(s) 201, 566, 594, 609, 714, 912, 1703, 1731
TspRI CASTG 4 cut(s) 714, 849, 1314, 1726
VspI ATTAAT 1 cut(s) 1635
XapI RAATTY 6 cut(s) 59, 151, 640, 1221, 1426, 1442
XceI RCATGY 2 cut(s) 873, 1009
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
XmaJI CCTAGG 1 cut(s) 791
XmiI GTMKAC 2 cut(s) 298, 1055
XspI CTAG 5 cut(s) 278, 584, 792, 1346, 1448
Zsp2I ATGCAT 3 cut(s) 196, 625, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.