RLG00000012825

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013247
Physical Location & Seq
Reverse (-)
22761747 .. 22764916
3170 bp
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UTR
Exon/CDS
Intron
RLM00000012825

Sequence Viewer

Length: 828 bp
ATGGGTCAAACCGGGGCATTGGATAGGGCGGAGAAGCTCCGAAAGGACGGCAACTCCTACTTCAAAAAGGGTAAATTCTTGGCCGCCATCGATGCCTACACTGAGGCAATCACGCTGTGCCCGAAAGTCCTTGTTAATTATACCAATCGTGCTCAGTGTCATCGCGTTCTTAAGGATTGGACCAGAGTAGAAGAAGATTCCAGGAAAGCTATTCAGCTCGACAGTGATTGGGTCAAGGGCCACTATTATCTGGGGCTTGCACTACTGCAGAAGCAAGAATATCCTGAAGGTGTTAAGGAATTGGAGAGGGCTTTGGATCTTGGGAGAGGTGCTGATCCTAAGGGTTATATGGTAGAAAACATATGGCAGGCGCTTGCGGAGGCAAAATACAAGGAGTGGGAAGAGGCATCAAGTAAACGTGCATGGGAATTGCAAAGCTTGAAAGAAGCTTGTGATAGTGCTCTCAGAGAGAAACATGATGCTTTTCAGATGGAAGGGTTTCTTGATGAAGATGGTACTACCCATATGAAACAGTTAGAGGCTTTAGGAAGAGTGTTTGAAAAAGCTGCAGAGGCTGACACAGCAAGTGAGGTGCCCGATTATTTATGTTGTAATATCTCACTTGAGATATTTCGTGATCCTGTAATTACACCAAGTGGTGTTACATATGAGAGATCAGTGATCCTGGACCATCTTGAGAAGGTGGGGAAGTTTGATCCTGTTACTCGTGAGCCAGTTGAACCGTCGCAGCTAATACCAAACTTTGCCATAAAAGAAGCAGTCCAAGCTTATTTAAAAGAACATGGTTGGGCCTATAGAACGTCGTAA

Protein Analysis

276

Amino Acids

31.33

Weight (kDa)

5.35

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 198 - 270 1.6e-28 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07370
fragaria_vesca FvH4_2g18060 FvH4_2g18060
malus_domestica MD05G1165100.v1.1 MD10G1154400.v1.1
prunus_persica Prupe.8G193300_v2.0.a1
pyrus_communis pycom05g15370 pycom10g13350
rosa_chinensis RchiOBHm_Chr6g0283451
rosa_laevigata RLG00000012825
rosa_multiflora Rmu_sc0004959.1_g000017
rosa_roxburghii Rroxscaffold_7G00185530
rosa_rugosa Rorug06G0154300
rosa_samantha Rh6AG266600 Rh6BG269100 Rh6CG269000 Rh6DG262400
rosa_wichuraiana Rw6G023080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 592
AccII CGCG 1 cut(s) 165
AciI CCGC 3 cut(s) 29, 84, 377
AclWI GGATC 5 cut(s) 324, 329, 632, 676, 710
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 74
AcuI CTGAAG 1 cut(s) 306
AdeI CACNNNGTG 2 cut(s) 117, 656
AfaI GTAC 1 cut(s) 517
AflII CTTAAG 1 cut(s) 170
AgsI TTSAA 4 cut(s) 64, 442, 560, 740
AjnI CCWGG 2 cut(s) 200, 684
AluBI AGCT 8 cut(s) 37, 209, 217, 438, 449, 566, 751, 788
AluI AGCT 8 cut(s) 37, 209, 217, 438, 449, 566, 751, 788
Alw21I GWGCWC 2 cut(s) 154, 463
AlwI GGATC 5 cut(s) 324, 329, 632, 676, 710
AlwNI CAGNNNCTG 1 cut(s) 575
AoxI GGCC 3 cut(s) 81, 238, 810
ApeKI GCWGC 2 cut(s) 566, 748
ApoI RAATTY 1 cut(s) 74
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 4 cut(s) 180, 238, 688, 810
AsuC2I CCSGG 1 cut(s) 13
AvaII GGWCC 2 cut(s) 180, 688
AxyI CCTNAGG 1 cut(s) 339
BaeGI GKGCMC 2 cut(s) 122, 597
BanI GGYRCC 1 cut(s) 592
BauI CACGAG 1 cut(s) 726
Bbv12I GWGCWC 2 cut(s) 154, 463
BbvI GCAGC 2 cut(s) 553, 760
BccI CCATC 4 cut(s) 95, 484, 506, 699
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 2 cut(s) 202, 686
BcnI CCSGG 1 cut(s) 13
BfmI CTRYAG 3 cut(s) 266, 567, 814
BfoI RGCGCY 1 cut(s) 374
BfrI CTTAAG 1 cut(s) 170
BisI GCNGC 3 cut(s) 84, 567, 749
BlsI GCNGC 3 cut(s) 85, 568, 750
Bme1390I CCNGG 3 cut(s) 13, 202, 686
Bme18I GGWCC 2 cut(s) 180, 688
BmgT120I GGNCC 4 cut(s) 180, 238, 688, 810
BmiI GGNNCC 1 cut(s) 594
BmrFI CCNGG 3 cut(s) 13, 202, 686
BmsI GCATC 3 cut(s) 82, 416, 469
BpuEI CTTGAG 2 cut(s) 644, 716
BpuMI CCSGG 1 cut(s) 13
Bsa29I ATCGAT 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 12
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bse1I ACTGG 1 cut(s) 734
Bse21I CCTNAGG 1 cut(s) 339
BseBI CCWGG 2 cut(s) 202, 686
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 93, 167, 478
BseNI ACTGG 1 cut(s) 734
BseSI GKGCMC 2 cut(s) 122, 597
BseXI GCAGC 2 cut(s) 553, 760
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 3 cut(s) 83, 240, 812
BshNI GGYRCC 1 cut(s) 592
BshVI ATCGAT 1 cut(s) 90
BsiHKAI GWGCWC 2 cut(s) 154, 463
BsiSI CCGG 1 cut(s) 12
BsnI GGCC 3 cut(s) 83, 240, 812
Bsp1286I GDGCHC 4 cut(s) 122, 154, 463, 597
Bsp143I GATC 6 cut(s) 316, 334, 637, 674, 681, 715
BspACI CCGC 3 cut(s) 29, 84, 377
BspANI GGCC 3 cut(s) 83, 240, 812
BspCNI CTCAG 3 cut(s) 94, 166, 477
BspDI ATCGAT 1 cut(s) 90
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 594
BspMAI CTGCAG 2 cut(s) 270, 571
BspPI GGATC 5 cut(s) 324, 329, 632, 676, 710
BspT107I GGYRCC 1 cut(s) 592
BspTI CTTAAG 1 cut(s) 170
BsrI ACTGG 1 cut(s) 734
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 6 cut(s) 316, 334, 637, 674, 681, 715
BssSI CACGAG 1 cut(s) 726
Bst2BI CACGAG 1 cut(s) 726
Bst2UI CCWGG 2 cut(s) 202, 686
Bst4CI ACNGT 3 cut(s) 224, 534, 744
Bst6I CTCTTC 2 cut(s) 396, 544
BstAFI CTTAAG 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 258, 369, 375
BstDEI CTNAG 4 cut(s) 102, 153, 339, 464
BstFNI CGCG 1 cut(s) 165
BstH2I RGCGCY 1 cut(s) 374
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 6 cut(s) 319, 337, 640, 677, 684, 718
BstMBI GATC 6 cut(s) 316, 334, 637, 674, 681, 715
BstMWI GCNNNNNNNGC 4 cut(s) 92, 572, 581, 785
BstNI CCWGG 2 cut(s) 202, 686
BstSCI CCNGG 3 cut(s) 11, 200, 684
BstSFI CTRYAG 3 cut(s) 266, 567, 814
BstSLI GKGCMC 2 cut(s) 122, 597
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 553, 760
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 90
Bsu36I CCTNAGG 1 cut(s) 339
BsuRI GGCC 3 cut(s) 83, 240, 812
BsuTUI ATCGAT 1 cut(s) 90
BtgZI GCGATG 1 cut(s) 146
BtsIMutI CAGTG 4 cut(s) 99, 161, 229, 684
Cac8I GCNNGC 3 cut(s) 258, 369, 375
CaiI CAGNNNCTG 1 cut(s) 575
CfoI GCGC 1 cut(s) 373
Cfr13I GGNCC 4 cut(s) 180, 238, 688, 810
ClaI ATCGAT 1 cut(s) 90
Csp6I GTAC 1 cut(s) 516
CviAII CATG 3 cut(s) 423, 476, 803
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 4 cut(s) 102, 153, 339, 464
DpnI GATC 6 cut(s) 318, 336, 639, 676, 683, 717
DpnII GATC 6 cut(s) 316, 334, 637, 674, 681, 715
DraI TTTAAA 1 cut(s) 795
DraIII CACNNNGTG 2 cut(s) 117, 656
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 396, 544
EarI CTCTTC 2 cut(s) 396, 544
EciI GGCGGA 1 cut(s) 44
Eco47I GGWCC 2 cut(s) 180, 688
Eco57I CTGAAG 1 cut(s) 306
Eco81I CCTNAGG 1 cut(s) 339
EcoRII CCWGG 2 cut(s) 200, 684
FaeI CATG 3 cut(s) 426, 479, 806
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FatI CATG 3 cut(s) 422, 475, 802
FauNDI CATATG 3 cut(s) 362, 525, 667
Fnu4HI GCNGC 3 cut(s) 84, 567, 749
Fsp4HI GCNGC 3 cut(s) 84, 567, 749
GlaI GCGC 1 cut(s) 372
GluI GCNGC 3 cut(s) 84, 567, 749
HaeII RGCGCY 1 cut(s) 374
HaeIII GGCC 3 cut(s) 83, 240, 812
HapII CCGG 1 cut(s) 12
HhaI GCGC 1 cut(s) 373
Hin1II CATG 3 cut(s) 426, 479, 806
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HindIII AAGCTT 3 cut(s) 436, 447, 786
HinfI GANTC 1 cut(s) 197
HpaII CCGG 1 cut(s) 12
Hpy166II GTNNAC 1 cut(s) 416
Hpy188I TCNGA 3 cut(s) 41, 467, 489
Hpy188III TCNNGA 5 cut(s) 284, 503, 635, 695, 728
Hpy8I GTNNAC 1 cut(s) 416
Hpy99I CGWCG 2 cut(s) 748, 826
HpyAV CCTTC 3 cut(s) 281, 488, 694
HpyCH4III ACNGT 3 cut(s) 224, 534, 744
HpyCH4IV ACGT 2 cut(s) 418, 821
HpyCH4V TGCA 5 cut(s) 260, 268, 422, 433, 569
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 572, 581, 785
HpyF3I CTNAG 4 cut(s) 102, 153, 339, 464
HpySE526I ACGT 2 cut(s) 418, 821
Hsp92II CATG 3 cut(s) 426, 479, 806
HspAI GCGC 1 cut(s) 371
Kzo9I GATC 6 cut(s) 316, 334, 637, 674, 681, 715
LmnI GCTCC 1 cut(s) 42
Lsp1109I GCAGC 2 cut(s) 553, 760
LweI GCATC 3 cut(s) 82, 416, 469
MaeII ACGT 2 cut(s) 418, 821
MaeIII GTNAC 2 cut(s) 661, 721
MalI GATC 6 cut(s) 318, 336, 639, 676, 683, 717
MboI GATC 6 cut(s) 316, 334, 637, 674, 681, 715
MboII GAAGA 5 cut(s) 203, 206, 413, 521, 561
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 4 cut(s) 122, 154, 463, 597
MluCI AATT 5 cut(s) 74, 136, 299, 428, 645
MnlI CCTC 8 cut(s) 97, 300, 320, 373, 397, 532, 565, 583
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 4 cut(s) 135, 171, 294, 794
MspCI CTTAAG 1 cut(s) 170
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 3 cut(s) 13, 202, 686
MvaI CCWGG 2 cut(s) 202, 686
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 4 cut(s) 92, 572, 581, 785
NciI CCSGG 1 cut(s) 13
NdeI CATATG 3 cut(s) 362, 525, 667
NdeII GATC 6 cut(s) 316, 334, 637, 674, 681, 715
NlaIII CATG 3 cut(s) 426, 479, 806
NlaIV GGNNCC 1 cut(s) 594
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 197
PfoI TCCNGGA 2 cut(s) 200, 684
PkrI GCNGC 3 cut(s) 85, 568, 750
Psp6I CCWGG 2 cut(s) 200, 684
PspGI CCWGG 2 cut(s) 200, 684
PspN4I GGNNCC 1 cut(s) 594
PspPI GGNCC 4 cut(s) 180, 238, 688, 810
PstI CTGCAG 2 cut(s) 270, 571
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 1 cut(s) 316
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
SaqAI TTAA 4 cut(s) 135, 171, 294, 794
SatI GCNGC 3 cut(s) 84, 567, 749
Sau3AI GATC 6 cut(s) 316, 334, 637, 674, 681, 715
Sau96I GGNCC 4 cut(s) 180, 238, 688, 810
ScrFI CCNGG 3 cut(s) 13, 202, 686
SduI GDGCHC 4 cut(s) 122, 154, 463, 597
SfaNI GCATC 3 cut(s) 82, 416, 469
SfcI CTRYAG 3 cut(s) 266, 567, 814
SinI GGWCC 2 cut(s) 180, 688
SmlI CTYRAG 3 cut(s) 170, 623, 695
SmoI CTYRAG 3 cut(s) 170, 623, 695
Sse9I AATT 5 cut(s) 74, 136, 299, 428, 645
SsiI CCGC 3 cut(s) 29, 84, 377
StyD4I CCNGG 3 cut(s) 11, 200, 684
TaaI ACNGT 3 cut(s) 224, 534, 744
TaiI ACGT 2 cut(s) 421, 824
TaqI TCGA 2 cut(s) 90, 219
TasI AATT 5 cut(s) 74, 136, 299, 428, 645
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 4 cut(s) 135, 171, 294, 794
Tru9I TTAA 4 cut(s) 135, 171, 294, 794
TscAI CASTG 4 cut(s) 106, 161, 229, 684
TseI GCWGC 2 cut(s) 566, 748
TspDTI ATGAA 2 cut(s) 522, 542
TspRI CASTG 4 cut(s) 106, 161, 229, 684
Vha464I CTTAAG 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 180, 688
XapI RAATTY 1 cut(s) 74
XmnI GAANNNNTTC 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.