RchiOBHm_Chr6g0283451

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013247
Physical Location & Seq
Forward (+)
46651914 .. 46656266
4353 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25415

Sequence Viewer

Length: 1170 bp
ATGAGAGCCCCCGTCTTGAGTCTTGACAGCAAAATAGGAGAGAAAAAAAAGGGCAAAAAAATAAGGGAACCCTTAAAAAAATCTCGAATCTTCCAATGTTATGTGACCTTGTTAGGGAAGGCTTCAATTTCTTTTCTTTTTATTTTTGGTTTTTTGAAAAGGGGCCAATATAGGCCAATATATAAACTAGTCTTGGTGCTTGACTCCGCTTTTAAGGTCATTTCTTCTTCCTTTCGCGCTTGCTTCCTGAAAACGAAGCCTCCTCTTCGCGCTTCCTCCTCTTCCCTAACACTCACTCAATTTGAGCCGGAGAGAGCCACACAGAGAGAGAGAGAGAGAGAGATGGGTCAAACCGGGGCATTGGATAGGGCGGAGAAGCTCCGGAAGGACGGCAACACCTACTTCAAAAGGGGTAAATACTTGGCCGCCATTGATGCCTACACTGAGGCAATCACACTGTGCCCTAAAGTCCTCGTTAATTATACCAATCGTGCTCAGTGTCATCGCGTTCTTAAGGATTGGACCAGAGTCGAAGAAGATTCCAGGAAAGCTATTCAGCTCGATAGTGATTGGGTCAAGGGCCACTATTATCTGGGGCTTGCACTACTGCAGAAGCAAGAATATCCTGAAGGAGTTAAGGAATTGGAGAGGGCTTTGGATCTTGGGAGAGTTGCTGATCCTAAGGGTTATATGGTAGAAGACATATGGCAGGAGCTTGCAAAGGCAAAATACAGGGAGTGGGAAGAGGCATCAAGTAAACGTGCATGGGAATTGCAAAGCTTGAAAGAAGCTTGTGAAAGTGCTCTCAGAGAGACACATGATGCTTTTCAGATGGAAGGGTTTCTGGATGAAGATGGTACTACCCATATGAAACAGTTAGAGGCTTTAGGAAGAGTGTTTGAAAAAGCTGCGGAAGCTGACACAGCAAGTGAGGTGCCCGATTATTTATGTTGTAATATCTCACTTGATATATTTCGTGATCCTGTAATTACACCAAGTGGTGTTACATATGAGAGATCAGTGATCCTGGACCATCTTGAGAAGGTGGGTAAGTTTGATCCTGTTACTCGTGAGCCAGTTGAACCGTCGCAGCTAATACCAAACTTTGCCATAAAAGAAGCAGTCCAAGCTTATTTAAAAGAACATGGTTGGGCCTATAGAACGTCGTAA

Protein Analysis

389

Amino Acids

44.6

Weight (kDa)

8.73

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 312 - 384 1.3e-27 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013247)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07370
fragaria_vesca FvH4_2g18060 FvH4_2g18060
malus_domestica MD05G1165100.v1.1 MD10G1154400.v1.1
prunus_persica Prupe.8G193300_v2.0.a1
pyrus_communis pycom05g15370 pycom10g13350
rosa_chinensis RchiOBHm_Chr6g0283451
rosa_laevigata RLG00000012825
rosa_multiflora Rmu_sc0004959.1_g000017
rosa_roxburghii Rroxscaffold_7G00185530
rosa_rugosa Rorug06G0154300
rosa_samantha Rh6AG266600 Rh6BG269100 Rh6CG269000 Rh6DG262400
rosa_wichuraiana Rw6G023080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 934
AccII CGCG 3 cut(s) 237, 270, 507
AccIII TCCGGA 1 cut(s) 381
AciI CCGC 4 cut(s) 207, 371, 426, 911
AclWI GGATC 5 cut(s) 666, 671, 974, 1018, 1052
AcoI YGGCCR 1 cut(s) 423
AcuI CTGAAG 1 cut(s) 648
AdeI CACNNNGTG 2 cut(s) 459, 998
AfaI GTAC 1 cut(s) 859
AfiI CCNNNNNNNGG 1 cut(s) 114
AflII CTTAAG 1 cut(s) 512
AgsI TTSAA 6 cut(s) 126, 157, 406, 784, 902, 1082
AhlI ACTAGT 1 cut(s) 187
AjnI CCWGG 2 cut(s) 542, 1026
Alw21I GWGCWC 2 cut(s) 496, 805
Alw26I GTCTC 1 cut(s) 806
AlwI GGATC 5 cut(s) 666, 671, 974, 1018, 1052
Aor13HI TCCGGA 1 cut(s) 381
AoxI GGCC 5 cut(s) 163, 173, 423, 580, 1152
ApeKI GCWGC 2 cut(s) 908, 1090
Asp700I GAANNNNTTC 1 cut(s) 840
AspLEI GCGC 2 cut(s) 239, 272
AspS9I GGNCC 5 cut(s) 163, 522, 580, 1030, 1152
AsuC2I CCSGG 1 cut(s) 355
AvaII GGWCC 2 cut(s) 522, 1030
AxyI CCTNAGG 1 cut(s) 681
BaeGI GKGCMC 2 cut(s) 464, 939
BanI GGYRCC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 10
BauI CACGAG 1 cut(s) 1068
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 2 cut(s) 496, 805
BbvI GCAGC 2 cut(s) 895, 1102
BccI CCATC 4 cut(s) 337, 826, 848, 1041
BceAI ACGGC 1 cut(s) 406
BciT130I CCWGG 2 cut(s) 544, 1028
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 806
BcuI ACTAGT 1 cut(s) 187
BfaI CTAG 1 cut(s) 188
BfmI CTRYAG 2 cut(s) 608, 1156
BfrI CTTAAG 1 cut(s) 512
BisI GCNGC 3 cut(s) 426, 909, 1091
BlsI GCNGC 3 cut(s) 427, 910, 1092
Bme1390I CCNGG 3 cut(s) 355, 544, 1028
Bme18I GGWCC 2 cut(s) 522, 1030
BmgT120I GGNCC 5 cut(s) 163, 522, 580, 1030, 1152
BmiI GGNNCC 3 cut(s) 69, 164, 936
BmrFI CCNGG 3 cut(s) 355, 544, 1028
BmsI GCATC 3 cut(s) 424, 758, 811
BoxI GACNNNNGTC 1 cut(s) 527
BpiI GAAGAC 1 cut(s) 705
BpuEI CTTGAG 2 cut(s) 37, 1058
BpuMI CCSGG 1 cut(s) 355
BsaJI CCNNGG 1 cut(s) 354
BsaWI WCCGGW 1 cut(s) 381
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 1076
Bse21I CCTNAGG 1 cut(s) 681
BseAI TCCGGA 1 cut(s) 381
BseBI CCWGG 2 cut(s) 544, 1028
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 853
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMII CTCAG 3 cut(s) 435, 509, 820
BseNI ACTGG 1 cut(s) 1076
BseRI GAGGAG 2 cut(s) 252, 268
BseSI GKGCMC 2 cut(s) 464, 939
BseXI GCAGC 2 cut(s) 895, 1102
Bsh1236I CGCG 3 cut(s) 237, 270, 507
BshFI GGCC 5 cut(s) 165, 175, 425, 582, 1154
BshNI GGYRCC 1 cut(s) 934
BsiHKAI GWGCWC 2 cut(s) 496, 805
BsiSI CCGG 3 cut(s) 308, 354, 382
BslI CCNNNNNNNGG 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 806
BsnI GGCC 5 cut(s) 165, 175, 425, 582, 1154
Bsp1286I GDGCHC 5 cut(s) 10, 464, 496, 805, 939
Bsp13I TCCGGA 1 cut(s) 381
Bsp143I GATC 6 cut(s) 658, 676, 979, 1016, 1023, 1057
BspACI CCGC 4 cut(s) 207, 371, 426, 911
BspANI GGCC 5 cut(s) 165, 175, 425, 582, 1154
BspCNI CTCAG 3 cut(s) 436, 508, 819
BspEI TCCGGA 1 cut(s) 381
BspFNI CGCG 3 cut(s) 237, 270, 507
BspLI GGNNCC 3 cut(s) 69, 164, 936
BspMAI CTGCAG 1 cut(s) 612
BspPI GGATC 5 cut(s) 666, 671, 974, 1018, 1052
BspT107I GGYRCC 1 cut(s) 934
BspTI CTTAAG 1 cut(s) 512
BsrI ACTGG 1 cut(s) 1076
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 6 cut(s) 658, 676, 979, 1016, 1023, 1057
BssSI CACGAG 1 cut(s) 1068
Bst2BI CACGAG 1 cut(s) 1068
Bst2UI CCWGG 2 cut(s) 544, 1028
Bst4CI ACNGT 3 cut(s) 459, 876, 1086
Bst6I CTCTTC 4 cut(s) 270, 286, 738, 886
BstAFI CTTAAG 1 cut(s) 512
BstC8I GCNNGC 3 cut(s) 241, 600, 717
BstDEI CTNAG 4 cut(s) 444, 495, 681, 806
BstF5I GGATG 1 cut(s) 853
BstFNI CGCG 3 cut(s) 237, 270, 507
BstHHI GCGC 2 cut(s) 239, 272
BstKTI GATC 6 cut(s) 661, 679, 982, 1019, 1026, 1060
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 6 cut(s) 658, 676, 979, 1016, 1023, 1057
BstMWI GCNNNNNNNGC 4 cut(s) 434, 914, 923, 1127
BstNI CCWGG 2 cut(s) 544, 1028
BstPAI GACNNNNGTC 1 cut(s) 527
BstSCI CCNGG 3 cut(s) 353, 542, 1026
BstSFI CTRYAG 2 cut(s) 608, 1156
BstSLI GKGCMC 2 cut(s) 464, 939
BstUI CGCG 3 cut(s) 237, 270, 507
BstV1I GCAGC 2 cut(s) 895, 1102
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 5 cut(s) 165, 175, 425, 582, 1154
BtgZI GCGATG 1 cut(s) 488
BtsCI GGATG 1 cut(s) 853
BtsIMutI CAGTG 4 cut(s) 441, 455, 503, 1026
Cac8I GCNNGC 3 cut(s) 241, 600, 717
CfoI GCGC 2 cut(s) 239, 272
Cfr13I GGNCC 5 cut(s) 163, 522, 580, 1030, 1152
Csp6I GTAC 1 cut(s) 858
CviAII CATG 3 cut(s) 765, 818, 1145
CviQI GTAC 1 cut(s) 858
DdeI CTNAG 4 cut(s) 444, 495, 681, 806
DpnI GATC 6 cut(s) 660, 678, 981, 1018, 1025, 1059
DpnII GATC 6 cut(s) 658, 676, 979, 1016, 1023, 1057
DraI TTTAAA 1 cut(s) 1137
DraIII CACNNNGTG 2 cut(s) 459, 998
EaeI YGGCCR 1 cut(s) 423
Eam1104I CTCTTC 4 cut(s) 270, 286, 738, 886
EarI CTCTTC 4 cut(s) 270, 286, 738, 886
EciI GGCGGA 1 cut(s) 386
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 2 cut(s) 522, 1030
Eco57I CTGAAG 1 cut(s) 648
Eco81I CCTNAGG 1 cut(s) 681
EcoRII CCWGG 2 cut(s) 542, 1026
EcoT38I GRGCYC 1 cut(s) 10
FaeI CATG 3 cut(s) 768, 821, 1148
FatI CATG 3 cut(s) 764, 817, 1144
FauNDI CATATG 3 cut(s) 704, 867, 1009
Fnu4HI GCNGC 3 cut(s) 426, 909, 1091
FokI GGATG 1 cut(s) 860
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 3 cut(s) 426, 909, 1091
FspBI CTAG 1 cut(s) 188
GlaI GCGC 2 cut(s) 238, 271
GluI GCNGC 3 cut(s) 426, 909, 1091
HaeIII GGCC 5 cut(s) 165, 175, 425, 582, 1154
HapII CCGG 3 cut(s) 308, 354, 382
HhaI GCGC 2 cut(s) 239, 272
Hin1II CATG 3 cut(s) 768, 821, 1148
Hin6I GCGC 2 cut(s) 237, 270
HinP1I GCGC 2 cut(s) 237, 270
HindIII AAGCTT 3 cut(s) 778, 789, 1128
HinfI GANTC 5 cut(s) 19, 87, 203, 528, 539
HpaII CCGG 3 cut(s) 308, 354, 382
Hpy166II GTNNAC 1 cut(s) 758
Hpy188I TCNGA 2 cut(s) 809, 831
Hpy8I GTNNAC 1 cut(s) 758
Hpy99I CGWCG 2 cut(s) 1090, 1168
HpyAV CCTTC 5 cut(s) 112, 379, 623, 830, 1036
HpyCH4III ACNGT 3 cut(s) 459, 876, 1086
HpyCH4IV ACGT 2 cut(s) 760, 1163
HpyCH4V TGCA 5 cut(s) 602, 610, 719, 764, 775
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 914, 923, 1127
HpyF3I CTNAG 4 cut(s) 444, 495, 681, 806
HpySE526I ACGT 2 cut(s) 760, 1163
Hsp92II CATG 3 cut(s) 768, 821, 1148
HspAI GCGC 2 cut(s) 237, 270
Kpn2I TCCGGA 1 cut(s) 381
Kzo9I GATC 6 cut(s) 658, 676, 979, 1016, 1023, 1057
LmnI GCTCC 2 cut(s) 384, 712
Lsp1109I GCAGC 2 cut(s) 895, 1102
LweI GCATC 3 cut(s) 424, 758, 811
MaeI CTAG 1 cut(s) 188
MaeII ACGT 2 cut(s) 760, 1163
MaeIII GTNAC 3 cut(s) 103, 1003, 1063
MalI GATC 6 cut(s) 660, 678, 981, 1018, 1025, 1059
MboI GATC 6 cut(s) 658, 676, 979, 1016, 1023, 1057
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 5 cut(s) 10, 464, 496, 805, 939
MluCI AATT 6 cut(s) 126, 299, 478, 641, 770, 987
MlyI GAGTC 3 cut(s) 28, 197, 537
MroI TCCGGA 1 cut(s) 381
MroXI GAANNNNTTC 1 cut(s) 840
MseI TTAA 6 cut(s) 74, 213, 477, 513, 636, 1136
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 3 cut(s) 308, 354, 382
MspR9I CCNGG 3 cut(s) 355, 544, 1028
MvaI CCWGG 2 cut(s) 544, 1028
MvnI CGCG 3 cut(s) 237, 270, 507
MwoI GCNNNNNNNGC 4 cut(s) 434, 914, 923, 1127
NciI CCSGG 1 cut(s) 355
NdeI CATATG 3 cut(s) 704, 867, 1009
NdeII GATC 6 cut(s) 658, 676, 979, 1016, 1023, 1057
NlaIII CATG 3 cut(s) 768, 821, 1148
NlaIV GGNNCC 3 cut(s) 69, 164, 936
NmuCI GTSAC 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 840
PfeI GAWTC 2 cut(s) 87, 539
PfoI TCCNGGA 2 cut(s) 542, 1026
PkrI GCNGC 3 cut(s) 427, 910, 1092
PleI GAGTC 3 cut(s) 27, 197, 536
PpsI GAGTC 3 cut(s) 27, 197, 536
PshAI GACNNNNGTC 1 cut(s) 527
Psp6I CCWGG 2 cut(s) 542, 1026
PspGI CCWGG 2 cut(s) 542, 1026
PspN4I GGNNCC 3 cut(s) 69, 164, 936
PspPI GGNCC 5 cut(s) 163, 522, 580, 1030, 1152
PstI CTGCAG 1 cut(s) 612
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 1 cut(s) 859
RsaNI GTAC 1 cut(s) 858
SaqAI TTAA 6 cut(s) 74, 213, 477, 513, 636, 1136
SatI GCNGC 3 cut(s) 426, 909, 1091
Sau3AI GATC 6 cut(s) 658, 676, 979, 1016, 1023, 1057
Sau96I GGNCC 5 cut(s) 163, 522, 580, 1030, 1152
SchI GAGTC 3 cut(s) 28, 197, 537
ScrFI CCNGG 3 cut(s) 355, 544, 1028
SduI GDGCHC 5 cut(s) 10, 464, 496, 805, 939
SfaNI GCATC 3 cut(s) 424, 758, 811
SfcI CTRYAG 2 cut(s) 608, 1156
SinI GGWCC 2 cut(s) 522, 1030
SmlI CTYRAG 3 cut(s) 16, 512, 1037
SmoI CTYRAG 3 cut(s) 16, 512, 1037
SpeI ACTAGT 1 cut(s) 187
Sse9I AATT 6 cut(s) 126, 299, 478, 641, 770, 987
SsiI CCGC 4 cut(s) 207, 371, 426, 911
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 3 cut(s) 353, 542, 1026
TaaI ACNGT 3 cut(s) 459, 876, 1086
TaiI ACGT 2 cut(s) 763, 1166
TaqI TCGA 3 cut(s) 85, 531, 561
TasI AATT 6 cut(s) 126, 299, 478, 641, 770, 987
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 2 cut(s) 87, 539
Tru1I TTAA 6 cut(s) 74, 213, 477, 513, 636, 1136
Tru9I TTAA 6 cut(s) 74, 213, 477, 513, 636, 1136
TscAI CASTG 4 cut(s) 448, 462, 503, 1026
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 2 cut(s) 908, 1090
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 2 cut(s) 864, 884
TspRI CASTG 4 cut(s) 448, 462, 503, 1026
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 2 cut(s) 522, 1030
XmnI GAANNNNTTC 1 cut(s) 840
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.