pycom05g24920
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010362
Physical Location & Seq
Forward (+)
26400141 .. 26403373
3233 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 918 bp
ATGTGCAGCTTGTTCATGCCAACTACTAGGATGGAGAAATTTCAAGGTGATTTAACCGACATACTAAGAGCTGGTACTACTACTGCTACCGTTGGAGCTGATCCGTCTGATCATCAAGAACCTTACACCGACTCGTGGCACTTTCCATATGATCATCCCATGATATTCCCATCATCCTCATCAGCCATGGAAGAAGAAGAAGAAACTAAGGACAACTTTGGCGATCCATTTTTGTCGTACATGCGCGATCCGCTGCTTAACGAGCTAGACATATCAAGTTCCAGCCTTTTCAGCAGCCCCAATTGTACAGCTCGTGCGGAGGAAGGTGTTACAACTACTACTTTTGGTAGCGGTGGCGATGGCAGTCATCATGTACTTAGTCTTGGGCATCACAACCATCAAAGTGGGGGTGTTGTGGTTGATGGTGATCCAATAAAGAGGCCTTACAACATATTCTCACGGATGCTACAGATCTCTCCGAGTGCAAAGGTACCGGTGTCGCCGTGTGATTCTCCGGTGATGGGTGCATCAGTGTCGTCGTCTACTAGCGGGGTTAAAACATCGTCAGCTTCTGCTGCCATGGTTGCAAGCGACATGATTATTAATGGGAACAGCTCATCAGCAGCAAAAGGTTGCTTGCTCGAGAACACTGGGATGCAGATCTCATCTCCACGGAATCCGGGTATCAAGCGAAGGAAGAGCCAGGCAAAAAAGGTGGTGTGCATTCCAGCGCCAGCAGCTGCAAACAGCAGGCCTACTGGAGAAGTAGTTCCGTCTGATCTGTGGGCATGGAGAAAGTACGGTCAGAAACCCATCAAAGGGTCACCTTATCCAAGGGGTTACTATAGATGTAGCAGTTCAAAGGGTTGCTCAGCAAGGAAACAAGTAGAGCGTAGCCGGACGGATCGATCCTTCTAG

Protein Analysis

306

Amino Acids

32.83

Weight (kDa)

8.13

Isoelectric Point (pI)

60.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 260 - 303 1.2e-18 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccI GTMKAC 1 cut(s) 542
AccII CGCG 1 cut(s) 246
AciI CCGC 4 cut(s) 251, 317, 351, 549
AclWI GGATC 6 cut(s) 95, 218, 242, 422, 903, 912
AcsI RAATTY 1 cut(s) 38
AfaI GTAC 6 cut(s) 76, 239, 307, 375, 492, 800
AfiI CCNNNNNNNGG 4 cut(s) 135, 521, 818, 819
AgeI ACCGGT 1 cut(s) 493
AgsI TTSAA 2 cut(s) 44, 861
AjnI CCWGG 1 cut(s) 702
AloI GAACNNNNNNTCC 2 cut(s) 753, 785
AluBI AGCT 8 cut(s) 9, 71, 98, 265, 311, 569, 615, 740
AluI AGCT 8 cut(s) 9, 71, 98, 265, 311, 569, 615, 740
AlwI GGATC 6 cut(s) 95, 218, 242, 422, 903, 912
AlwNI CAGNNNCTG 2 cut(s) 572, 740
Ama87I CYCGRG 1 cut(s) 641
AoxI GGCC 2 cut(s) 440, 752
ApeKI GCWGC 7 cut(s) 6, 253, 294, 575, 623, 737, 740
ApoI RAATTY 1 cut(s) 38
AseI ATTAAT 1 cut(s) 603
AsiGI ACCGGT 1 cut(s) 493
Asp700I GAANNNNTTC 1 cut(s) 768
Asp718I GGTACC 1 cut(s) 490
AspLEI GCGC 2 cut(s) 246, 733
AsuC2I CCSGG 1 cut(s) 681
AsuHPI GGTGA 4 cut(s) 59, 437, 529, 816
AvaI CYCGRG 1 cut(s) 641
BaeI ACNNNNGTAYC 2 cut(s) 482, 515
BanI GGYRCC 1 cut(s) 490
BauI CACGAG 2 cut(s) 133, 312
BbvI GCAGC 7 cut(s) 18, 240, 306, 562, 635, 727, 749
BccI CCATC 7 cut(s) 25, 178, 353, 405, 416, 514, 821
BceAI ACGGC 1 cut(s) 487
BcgI CGANNNNNNTGC 2 cut(s) 516, 550
BciT130I CCWGG 1 cut(s) 704
BclI TGATCA 2 cut(s) 109, 151
BcnI CCSGG 1 cut(s) 681
BfaI CTAG 4 cut(s) 27, 266, 546, 916
BfmI CTRYAG 2 cut(s) 467, 844
BfoI RGCGCY 1 cut(s) 734
BglII AGATCT 2 cut(s) 471, 660
BisI GCNGC 7 cut(s) 7, 254, 295, 576, 624, 738, 741
BlpI GCTNAGC 1 cut(s) 871
BlsI GCNGC 7 cut(s) 8, 255, 296, 577, 625, 739, 742
Bme1390I CCNGG 2 cut(s) 681, 704
BmeT110I CYCGRG 1 cut(s) 641
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 2 cut(s) 681, 704
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 4 cut(s) 397, 453, 536, 645
BmuI ACTGGG 1 cut(s) 660
BpmI CTGGAG 1 cut(s) 780
Bpu1102I GCTNAGC 1 cut(s) 871
BpuMI CCSGG 1 cut(s) 681
Bsa29I ATCGAT 1 cut(s) 907
BsaBI GATNNNNATC 2 cut(s) 426, 659
BsaJI CCNNGG 4 cut(s) 186, 579, 671, 833
BsaWI WCCGGW 2 cut(s) 493, 514
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 521, 818, 819
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 2 cut(s) 655, 763
Bse8I GATNNNNATC 2 cut(s) 426, 659
BseBI CCWGG 1 cut(s) 704
BseCI ATCGAT 1 cut(s) 907
BseDI CCNNGG 4 cut(s) 186, 579, 671, 833
BseGI GGATG 5 cut(s) 36, 154, 173, 468, 660
BseJI GATNNNNATC 2 cut(s) 426, 659
BseLI CCNNNNNNNGG 4 cut(s) 135, 521, 818, 819
BseMII CTCAG 1 cut(s) 885
BseNI ACTGG 2 cut(s) 655, 763
BseXI GCAGC 7 cut(s) 18, 240, 306, 562, 635, 727, 749
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 2 cut(s) 442, 754
BshNI GGYRCC 1 cut(s) 490
BshTI ACCGGT 1 cut(s) 493
BshVI ATCGAT 1 cut(s) 907
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 4 cut(s) 494, 515, 680, 898
BslI CCNNNNNNNGG 4 cut(s) 135, 521, 818, 819
BsmI GAATGC 1 cut(s) 723
BsnI GGCC 2 cut(s) 442, 754
BsoBI CYCGRG 1 cut(s) 641
Bsp1407I TGTACA 1 cut(s) 305
Bsp1720I GCTNAGC 1 cut(s) 871
Bsp19I CCATGG 2 cut(s) 186, 579
BspACI CCGC 4 cut(s) 251, 317, 351, 549
BspANI GGCC 2 cut(s) 442, 754
BspCNI CTCAG 1 cut(s) 884
BspDI ATCGAT 1 cut(s) 907
BspFNI CGCG 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 492
BspPI GGATC 6 cut(s) 95, 218, 242, 422, 903, 912
BspQI GCTCTTC 1 cut(s) 692
BspT107I GGYRCC 1 cut(s) 490
BsrFI RCCGGY 1 cut(s) 493
BsrGI TGTACA 1 cut(s) 305
BsrI ACTGG 2 cut(s) 655, 763
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 4 cut(s) 186, 579, 671, 833
BssSI CACGAG 2 cut(s) 133, 312
BssT1I CCWWGG 3 cut(s) 186, 579, 833
Bst2BI CACGAG 2 cut(s) 133, 312
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 2 cut(s) 91, 803
Bst6I CTCTTC 1 cut(s) 692
BstAUI TGTACA 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 589, 638, 735, 752
BstDEI CTNAG 4 cut(s) 65, 207, 377, 871
BstDSI CCRYGG 3 cut(s) 186, 579, 671
BstEII GGTNACC 1 cut(s) 822
BstF5I GGATG 5 cut(s) 36, 154, 173, 468, 660
BstFNI CGCG 1 cut(s) 246
BstH2I RGCGCY 1 cut(s) 734
BstHHI GCGC 2 cut(s) 246, 733
BstMWI GCNNNNNNNGC 6 cut(s) 250, 262, 291, 575, 584, 737
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 1 cut(s) 244
BstPI GGTNACC 1 cut(s) 822
BstSCI CCNGG 2 cut(s) 679, 702
BstSFI CTRYAG 2 cut(s) 467, 844
BstUI CGCG 1 cut(s) 246
BstV1I GCAGC 7 cut(s) 18, 240, 306, 562, 635, 727, 749
BstX2I RGATCY 2 cut(s) 471, 660
BstXI CCANNNNNNTGG 1 cut(s) 404
BstYI RGATCY 2 cut(s) 471, 660
Bsu15I ATCGAT 1 cut(s) 907
BsuRI GGCC 2 cut(s) 442, 754
BsuTUI ATCGAT 1 cut(s) 907
BtgI CCRYGG 3 cut(s) 186, 579, 671
BtgZI GCGATG 1 cut(s) 372
BtsCI GGATG 5 cut(s) 36, 154, 173, 468, 660
BtsIMutI CAGTG 2 cut(s) 537, 648
Cac8I GCNNGC 4 cut(s) 589, 638, 735, 752
CaiI CAGNNNCTG 2 cut(s) 572, 740
CfoI GCGC 2 cut(s) 246, 733
Cfr10I RCCGGY 1 cut(s) 493
ClaI ATCGAT 1 cut(s) 907
Csp6I GTAC 6 cut(s) 75, 238, 306, 374, 491, 799
CspAI ACCGGT 1 cut(s) 493
CspCI CAANNNNNGTGG 2 cut(s) 696, 731
CviAII CATG 8 cut(s) 16, 160, 187, 241, 371, 580, 595, 789
CviQI GTAC 6 cut(s) 75, 238, 306, 374, 491, 799
DdeI CTNAG 4 cut(s) 65, 207, 377, 871
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
Eco130I CCWWGG 3 cut(s) 186, 579, 833
Eco147I AGGCCT 2 cut(s) 442, 754
Eco88I CYCGRG 1 cut(s) 641
Eco91I GGTNACC 1 cut(s) 822
EcoO65I GGTNACC 1 cut(s) 822
EcoRII CCWGG 1 cut(s) 702
EcoT14I CCWWGG 3 cut(s) 186, 579, 833
ErhI CCWWGG 3 cut(s) 186, 579, 833
FaeI CATG 8 cut(s) 19, 163, 190, 244, 374, 583, 598, 792
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FatI CATG 8 cut(s) 15, 159, 186, 240, 370, 579, 594, 788
FauI CCCGC 1 cut(s) 542
FauNDI CATATG 1 cut(s) 148
FbaI TGATCA 2 cut(s) 109, 151
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 7 cut(s) 7, 254, 295, 576, 624, 738, 741
FokI GGATG 5 cut(s) 43, 141, 160, 475, 667
Fsp4HI GCNGC 7 cut(s) 7, 254, 295, 576, 624, 738, 741
FspBI CTAG 4 cut(s) 27, 266, 546, 916
GlaI GCGC 2 cut(s) 245, 732
GluI GCNGC 7 cut(s) 7, 254, 295, 576, 624, 738, 741
GsuI CTGGAG 1 cut(s) 780
HaeII RGCGCY 1 cut(s) 734
HaeIII GGCC 2 cut(s) 442, 754
HapII CCGG 4 cut(s) 494, 515, 680, 898
HhaI GCGC 2 cut(s) 246, 733
Hin1II CATG 8 cut(s) 19, 163, 190, 244, 374, 583, 598, 792
Hin6I GCGC 2 cut(s) 244, 731
HinP1I GCGC 2 cut(s) 244, 731
HinfI GANTC 3 cut(s) 131, 509, 676
HpaII CCGG 4 cut(s) 494, 515, 680, 898
HphI GGTGA 4 cut(s) 59, 437, 529, 816
Hpy166II GTNNAC 1 cut(s) 543
Hpy188I TCNGA 4 cut(s) 109, 480, 778, 807
Hpy188III TCNNGA 2 cut(s) 116, 643
Hpy8I GTNNAC 1 cut(s) 543
Hpy99I CGWCG 1 cut(s) 541
HpyAV CCTTC 2 cut(s) 317, 687
HpyCH4III ACNGT 2 cut(s) 91, 803
HpyCH4V TGCA 7 cut(s) 6, 485, 527, 587, 658, 723, 743
HpyF10VI GCNNNNNNNGC 6 cut(s) 250, 262, 291, 575, 584, 737
HpyF3I CTNAG 4 cut(s) 65, 207, 377, 871
Hsp92II CATG 8 cut(s) 19, 163, 190, 244, 374, 583, 598, 792
HspAI GCGC 2 cut(s) 244, 731
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 2 cut(s) 109, 151
LguI GCTCTTC 1 cut(s) 692
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 7 cut(s) 18, 240, 306, 562, 635, 727, 749
LweI GCATC 4 cut(s) 397, 453, 536, 645
MaeI CTAG 4 cut(s) 27, 266, 546, 916
MaeIII GTNAC 3 cut(s) 328, 822, 839
MboII GAAGA 5 cut(s) 203, 206, 209, 212, 709
MfeI CAATTG 1 cut(s) 301
MflI RGATCY 2 cut(s) 471, 660
MluCI AATT 2 cut(s) 38, 301
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 73
MnlI CCTC 3 cut(s) 187, 313, 432
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 4 cut(s) 53, 258, 555, 603
MslI CAYNNNNRTG 2 cut(s) 402, 653
MspA1I CMGCKG 2 cut(s) 253, 740
MspI CCGG 4 cut(s) 494, 515, 680, 898
MspR9I CCNGG 2 cut(s) 681, 704
MunI CAATTG 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 723
MvaI CCWGG 1 cut(s) 704
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 6 cut(s) 250, 262, 291, 575, 584, 737
NciI CCSGG 1 cut(s) 681
NcoI CCATGG 2 cut(s) 186, 579
NdeI CATATG 1 cut(s) 148
NlaIII CATG 8 cut(s) 19, 163, 190, 244, 374, 583, 598, 792
NlaIV GGNNCC 1 cut(s) 492
NmuCI GTSAC 1 cut(s) 822
NspI RCATGY 1 cut(s) 244
PaeR7I CTCGAG 1 cut(s) 641
PceI AGGCCT 2 cut(s) 442, 754
PciSI GCTCTTC 1 cut(s) 692
PctI GAATGC 1 cut(s) 723
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 2 cut(s) 509, 676
PinAI ACCGGT 1 cut(s) 493
PkrI GCNGC 7 cut(s) 8, 255, 296, 577, 625, 739, 742
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PshBI ATTAAT 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 702
PspEI GGTNACC 1 cut(s) 822
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 1 cut(s) 492
PstNI CAGNNNCTG 2 cut(s) 572, 740
PsuI RGATCY 2 cut(s) 471, 660
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 6 cut(s) 76, 239, 307, 375, 492, 800
RsaNI GTAC 6 cut(s) 75, 238, 306, 374, 491, 799
RseI CAYNNNNRTG 2 cut(s) 402, 653
SapI GCTCTTC 1 cut(s) 692
SaqAI TTAA 4 cut(s) 53, 258, 555, 603
SatI GCNGC 7 cut(s) 7, 254, 295, 576, 624, 738, 741
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 681, 704
SfaNI GCATC 4 cut(s) 397, 453, 536, 645
SfcI CTRYAG 2 cut(s) 467, 844
Sfr274I CTCGAG 1 cut(s) 641
SlaI CTCGAG 1 cut(s) 641
SmiMI CAYNNNNRTG 2 cut(s) 402, 653
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 2 cut(s) 38, 301
SseBI AGGCCT 2 cut(s) 442, 754
SsiI CCGC 4 cut(s) 251, 317, 351, 549
SspMI CTAG 4 cut(s) 27, 266, 546, 916
StuI AGGCCT 2 cut(s) 442, 754
StyD4I CCNGG 2 cut(s) 679, 702
StyI CCWWGG 3 cut(s) 186, 579, 833
TaaI ACNGT 2 cut(s) 91, 803
TaqI TCGA 2 cut(s) 642, 907
TasI AATT 2 cut(s) 38, 301
TatI WGTACW 2 cut(s) 305, 373
TfiI GAWTC 2 cut(s) 509, 676
Tru1I TTAA 4 cut(s) 53, 258, 555, 603
Tru9I TTAA 4 cut(s) 53, 258, 555, 603
TscAI CASTG 2 cut(s) 537, 655
TseFI GTSAC 1 cut(s) 822
TseI GCWGC 7 cut(s) 6, 253, 294, 575, 623, 737, 740
Tsp45I GTSAC 1 cut(s) 822
TspDTI ATGAA 1 cut(s) 4
TspGWI ACGGA 5 cut(s) 93, 475, 688, 762, 917
TspRI CASTG 2 cut(s) 537, 655
VspI ATTAAT 1 cut(s) 603
XapI RAATTY 1 cut(s) 38
XceI RCATGY 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 641
XmiI GTMKAC 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 4 cut(s) 27, 266, 546, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.