pycom05g25390

Small RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002993
Physical Location & Seq
Reverse (-)
26756056 .. 26763608
7553 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3087 bp
ATGTGCGGTCCACTTGTGTTAGAGTCTTTGCTTTCCTTCCTCCAAAACCTCTCTCTCTCTCTCTCTCTCTCTATATCGTTTCTGCTGCCCCACCAAAACCCCACCAACGCACACATTATTCACTCTCTCTCTCTGCTCTGTGCCTCTCTCTCTCCCTCCTCTCTCAGGCTCGGCCATTGCCGTTGTGAATGCGAAAAGTTGAAGACCTCTTGGGTACAGATGGAAACTGAAGGAGCCGCAACTGTTGCAGTGCGAAACACTACCTTTACGCCTAAGGCTATCATACACAAAAAGTTCGGTAGCACAGCCTGCTATGAAGTAGAGGAAGTGCATGAGTCTCCTCAAAATGGATGTCCAGGGTTGGCCATTATGCAGAAGGGCCCTTGCCTTTATCGCTGTACCTTGAAACTTCCAGAAGTTACTGTCGTATCTGGAACCTTTAAAAAGAAGAAGGACGCAGAACAGTCTGCCGCTGAATTGGCCTTAGAAAAGCTAGGCATTAATCCCTCAACAAAAGTCCCTACTCTGCAGGAAGCATGGGATGAGTTAGTCTCTCGTGTCAATTTTTTATTTTCAGACGAGTTTCTTCCAGCTCTTCATCCTCTGAGTGGTCACTTCAGAGCAGCATTGCAGAGAGAAGGTGATCTTTCTGGTCAAATTCCTGCTGCAGTCATTGCTGTCTTTGACGCAACACTTTTTAATATGTGTAAATCCATTAATCCCAAGGTGGAGTCAATTCCTTTCTTGGCTATATTATATGTTATGAGGGCTGCTGCTAAATTATCTGGACTTCTTGCTACTTCTGAGGAAGATCTCTGGATTAGGAGGAAAAATCCATACACTCCTGAAATAATAGAGTCATCATCTGTTGAGCAATCAGACTCCAAAGAAATATTTCCGATTGAAGCCATAAATGTACCATGTTCATTAGAGAAGAGTGTTGAAAGAGTAATCCTAAATCTCTCATCTTCTGGATATTTCCTGGATGTTATAGCAAAACAACTTGGGTTATTGAACGCTGCTGACATTCTGATCTCAAGGCCTATAGGTAAAGCTTCCTCTGAGACAAGACTGTACTTTGCTGCTCCAAAGAAATCTCTGTTGGATATCTCTTCAGATCTTCTAAATGCCAAAGGAGCTTGTAATTCTGAAGGATCTTTCAATGCAAGAGCAAGTTACCTGTCGGGCCAAGATATATATGGTGACGCTGTTTTGGCATCCATTGGATACACATGGAGGTCCAAAGACCTTTTCTATGAAGATGTATCCCTGCAATCATATCACAGGATGGTCATTGGCAAGACACCAAGTGGAATTTACAAGTTGTCCAGAGAAGCAATACTTGCAGCTGAGTTGCCTTTAGCATTTACTGCTAAGGCAAAATGGAAGGGTTCGATTCCAAGGGAGATACTGTGTACATTCTGCCGCCAGCACCGGCTTGAGCCCATCTTCTCTCCTCAAAACACTCTAGAAGAATCATCCGAGTCACCAATATCACACAAGAAGTTAAAGGTGACAGACTTGGCTGGGAAAGAGACACAATATGCAAATGGCTGTGTTGTTGATGCTGGTGTAAAGGAGATAGTTGAATCCGGAGGTAGCTTTAGATGTGAAGTAAAAATAATTTCTAAGTTTCAGGATTTTATTTTAGAGTGCTCACCAAAAGAGACTTTTAAGAAGCAGAGTGATGCCATCCAGAATGTTTCTTTGAAAGTTCTGTTATGGTTAAATGCATATTTTGGGGATCTAACTATGCCTTTAGAGAGACTAAACGCTTCTGCTGATGGCCACAACATTAGATTCGATTCCCAAAACTTTATTAAAACATTTATGCTGTGCCATCATATTCATACTGTTGGGCATAAAGAGACTGAAGAAGGAAAATTATCATTTTCCAACTCTGTGAATGCACTGTTTGCTCTCCCTGGAATTGAAATTTGTTCCGTAAGTATAGAAGGACCGGATTCTGGTGTTTGTCCATCTATTGGGTCCTTAGCATGTGTAAGCTATTCTACTTCTTTGGTGACTGAAGGCAAACATACGAAAGAACTTTTGGAAAGTAGTGATGACTTTGAGTTTGAGATAGCATCTGGAGCAGTGATCCCACATCTCGAGTCAGTTATTACACAAATAACTGTTGGTCAGTCGGCTTTTTTCAGTATGGATTTGCCACCTCAAGAGTTGGTTTTAGCCGCAGCTGGTGATTCTGCTAGGATGCTTTCCTTATTGTCTTCAGAAACCTGCTATTTGGAGTACACTATAACTTTGTTGGGGGTAACCGAACCACTAGAGGATAGAATGGAGCAGGCTTTTTTCAGCCCTCCACTTTCAAAACAGCGTGTGGAATATGCAGTGCAAAGCATCAAAGAATCTTGTGCAACTACTTTGGTTGACTTTGGATGTGGCTCTGGTAGTTTGTTGGATTCTTTATTAAACTATCCAACTTCTCTGGAAAAAATTGCTGGTGTTGATATTTCACGAAAGAGTCTTGCTCGTGCGGCAAAGATACTTCATTCCAAATTAAACTCAAATTCAGATGCCATTACGTCAGCCATGAATTCAGCAATTCTTTACGATGGTTCAATTACAAATTTTGATTCCCGGTTGAGTGGATTTGATATTGGCACTTGCTTAGAGGTAATAGAGCATATGGAGGAAGATCAAGCATCTGAGTTTGGCAACGTGGTGCTAAGTTTATTTCGTCCAAGGGTTCTTATTGTCTCCACTCCAAACTACGAATACAATGTGATACTCCAGAAATCCAATTTGTCAAGCCAAGAAGATGACCCGGAGGACAAAAGTCAAGCACAATGTAAATTTCGTAACCATGACCACAAGTTCGAGTGGACGAGAGAGCAGTTCAATTGCTGGGCGACTGAATTGGCTGCGAAGCACAACTACAGTGTTGAGTTCAGTGGAGTTGGGGGTTCCGGTGATACAGAACCAGGTTTTGCTTCTCAGATTGCTGTCTTTAGACAGGAACACGTAACCAAGGAAGTTGCTTTTCCAGAGGTTTCAGATCTGGAACAACCTTTCGAAGTTATATGGGAATGGAATAGCAGCGATAGGCCAGGATCTGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001510 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009653 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010093 GO:0010305 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016740 GO:0016741 GO:0019222 GO:0022414 GO:0031047 GO:0031050 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035279 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048519 GO:0048580 GO:0048608 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0098542 GO:0098586 GO:0098795 GO:0099402 GO:0140098 GO:1901360 GO:1901698 GO:1901699 GO:1905392 GO:1905393 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKBP_C Methyltransf_11 Methyltransf_12 Methyltransf_31 dsrm

Protein Analysis

1029

Amino Acids

112.93

Weight (kDa)

5.28

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSRM_2 PF24995 96 - 166 6.5e-13 dsRNA binding domain
dsrm PF00035 130 - 165 9.9e-06 Double-stranded RNA binding motif
Hen1_LCD PF21224 178 - 280 3.9e-42 Small RNA 2'-O-methyltransferase-like, La-motif-containing domain
Hen1_Lam_C PF18441 301 - 434 7.4e-60 Hen1 La-motif C-terminal domain
dsRBD2 PF17842 436 - 580 6.3e-60 Double-stranded RNA binding domain 2
Methyltransf_23 PF13489 795 - 950 7.6e-06 Methyltransferase domain
Methyltransf_12 PF08242 797 - 891 7.3e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2249
AccB7I CCANNNNNTGG 1 cut(s) 1985
AccIII TCCGGA 1 cut(s) 1592
AciI CCGC 6 cut(s) 6, 237, 471, 1426, 2193, 2498
AclWI GGATC 4 cut(s) 1162, 1752, 2095, 3082
AcoI YGGCCR 3 cut(s) 172, 363, 1786
AcsI RAATTY 7 cut(s) 657, 1314, 1935, 2530, 2557, 2590, 2816
AcuI CTGAAG 7 cut(s) 249, 601, 1098, 1170, 1893, 2049, 2217
AdeI CACNNNGTG 1 cut(s) 1310
AfaI GTAC 6 cut(s) 216, 400, 918, 1076, 1417, 2255
AfiI CCNNNNNNNGG 5 cut(s) 165, 347, 608, 1967, 1985
AflIII ACRYGT 1 cut(s) 2983
AjnI CCWGG 5 cut(s) 355, 981, 1924, 2944, 3070
AjuI GAANNNNNNNTTGG 6 cut(s) 1085, 1117, 1235, 1267, 2036, 2068
AluBI AGCT 8 cut(s) 493, 593, 1055, 1139, 1349, 1602, 2007, 2198
AluI AGCT 8 cut(s) 493, 593, 1055, 1139, 1349, 1602, 2007, 2198
Alw21I GWGCWC 1 cut(s) 1658
Alw26I GTCTC 8 cut(s) 342, 556, 1058, 1529, 1661, 1759, 1862, 2725
AlwI GGATC 4 cut(s) 1162, 1752, 2095, 3082
AlwNI CAGNNNCTG 1 cut(s) 3077
Ama87I CYCGRG 1 cut(s) 2111
Aor13HI TCCGGA 1 cut(s) 1592
AoxI GGCC 8 cut(s) 172, 363, 379, 480, 1040, 1186, 1786, 3068
ApaI GGGCCC 1 cut(s) 383
ApoI RAATTY 7 cut(s) 657, 1314, 1935, 2530, 2557, 2590, 2816
ArsI GACNNNNNNTTYG 6 cut(s) 337, 369, 2060, 2092, 2822, 2854
AseI ATTAAT 2 cut(s) 501, 717
Asp700I GAANNNNTTC 1 cut(s) 894
AspS9I GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
AsuC2I CCSGG 2 cut(s) 2602, 2789
AsuHPI GGTGA 8 cut(s) 653, 1214, 1479, 1525, 1650, 2035, 2213, 2945
AsuII TTCGAA 1 cut(s) 3036
AvaI CYCGRG 1 cut(s) 2111
AvaII GGWCC 4 cut(s) 8, 1239, 1958, 1989
AxyI CCTNAGG 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 383
BaeI ACNNNNGTAYC 2 cut(s) 411, 444
BalI TGGCCA 2 cut(s) 365, 1788
BanII GRGCYC 2 cut(s) 383, 1446
BarI GAAGNNNNNNTAC 2 cut(s) 2882, 2914
BauI CACGAG 2 cut(s) 555, 2493
BbsI GAAGAC 2 cut(s) 209, 2223
Bbv12I GWGCWC 1 cut(s) 1658
BccI CCATC 8 cut(s) 214, 1282, 1454, 1700, 1778, 1848, 1987, 2570
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
BciVI GTATCC 2 cut(s) 1220, 1276
BcnI CCSGG 2 cut(s) 2602, 2789
BcoDI GTCTC 8 cut(s) 342, 556, 1058, 1529, 1661, 1759, 1862, 2725
BfaI CTAG 4 cut(s) 494, 1469, 2211, 2288
BfmI CTRYAG 4 cut(s) 527, 666, 1044, 2899
BfuAI ACCTGC 1 cut(s) 2249
BfuI GTATCC 2 cut(s) 1220, 1276
BglII AGATCT 3 cut(s) 811, 1117, 3019
Bme1390I CCNGG 7 cut(s) 357, 983, 1926, 2602, 2789, 2946, 3072
Bme18I GGWCC 4 cut(s) 8, 1239, 1958, 1989
BmeT110I CYCGRG 1 cut(s) 2111
BmgT120I GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
BmiI GGNNCC 5 cut(s) 235, 381, 436, 1990, 2929
BmrFI CCNGG 7 cut(s) 357, 983, 1926, 2602, 2789, 2946, 3072
BmsI GCATC 8 cut(s) 1226, 1555, 1678, 2096, 2205, 2370, 2527, 2675
BoxI GACNNNNGTC 1 cut(s) 2799
BpiI GAAGAC 2 cut(s) 209, 2223
BplI GAGNNNNNCTC 4 cut(s) 536, 568, 2476, 2508
BpmI CTGGAG 2 cut(s) 2112, 2738
Bpu10I CCTNAGC 2 cut(s) 1374, 1993
Bpu14I TTCGAA 1 cut(s) 3036
BpuEI CTTGAG 3 cut(s) 1021, 1460, 2160
BpuMI CCSGG 2 cut(s) 2602, 2789
BsaAI YACGTR 1 cut(s) 2986
BsaJI CCNNGG 6 cut(s) 356, 723, 1400, 1924, 2705, 2991
BsaWI WCCGGW 3 cut(s) 1592, 1960, 2930
BsaXI ACNNNNNCTCC 2 cut(s) 1397, 1427
Bsc4I CCNNNNNNNGG 5 cut(s) 165, 347, 608, 1967, 1985
Bse118I RCCGGY 1 cut(s) 1434
Bse21I CCTNAGG 1 cut(s) 273
Bse3DI GCAATG 3 cut(s) 175, 626, 672
BseAI TCCGGA 1 cut(s) 1592
BseBI CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
BseDI CCNNGG 6 cut(s) 356, 723, 1400, 1924, 2705, 2991
BseLI CCNNNNNNNGG 5 cut(s) 165, 347, 608, 1967, 1985
BseMI GCAATG 3 cut(s) 175, 626, 672
BseMII CTCAG 7 cut(s) 178, 596, 795, 1053, 1341, 2661, 2972
BseRI GAGGAG 3 cut(s) 148, 330, 1446
BseSI GKGCMC 1 cut(s) 383
BseYI CCCAGC 2 cut(s) 1526, 2868
BshFI GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
BsiHKAI GWGCWC 1 cut(s) 1658
BsiHKCI CYCGRG 1 cut(s) 2111
BsiSI CCGG 6 cut(s) 1435, 1593, 1961, 2602, 2789, 2931
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 5 cut(s) 165, 347, 608, 1967, 1985
BsmAI GTCTC 8 cut(s) 342, 556, 1058, 1529, 1661, 1759, 1862, 2725
BsmFI GGGAC 1 cut(s) 503
BsmI GAATGC 2 cut(s) 194, 1912
BsnI GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
BsoBI CYCGRG 1 cut(s) 2111
Bsp119I TTCGAA 1 cut(s) 3036
Bsp120I GGGCCC 1 cut(s) 379
Bsp1286I GDGCHC 3 cut(s) 383, 1446, 1658
Bsp13I TCCGGA 1 cut(s) 1592
Bsp1407I TGTACA 1 cut(s) 1415
BspACI CCGC 6 cut(s) 6, 237, 471, 1426, 2193, 2498
BspANI GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
BspCNI CTCAG 7 cut(s) 177, 597, 796, 1054, 1342, 2662, 2971
BspEI TCCGGA 1 cut(s) 1592
BspLI GGNNCC 5 cut(s) 235, 381, 436, 1990, 2929
BspMAI CTGCAG 2 cut(s) 531, 670
BspMI ACCTGC 1 cut(s) 2249
BspPI GGATC 4 cut(s) 1162, 1752, 2095, 3082
BspQI GCTCTTC 1 cut(s) 600
BspT104I TTCGAA 1 cut(s) 3036
BsrDI GCAATG 3 cut(s) 175, 626, 672
BsrFI RCCGGY 1 cut(s) 1434
BsrGI TGTACA 1 cut(s) 1415
BssAI RCCGGY 1 cut(s) 1434
BssECI CCNNGG 6 cut(s) 356, 723, 1400, 1924, 2705, 2991
BssSI CACGAG 2 cut(s) 555, 2493
BssT1I CCWWGG 4 cut(s) 723, 1400, 2705, 2991
Bst2BI CACGAG 2 cut(s) 555, 2493
Bst2UI CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
Bst4CI ACNGT 9 cut(s) 244, 424, 465, 1074, 1413, 1855, 1914, 2137, 2903
Bst6I CTCTTC 3 cut(s) 600, 929, 1117
BstAPI GCANNNNNTGC 6 cut(s) 245, 309, 674, 1343, 1370, 1916
BstAUI TGTACA 1 cut(s) 1415
BstBAI YACGTR 1 cut(s) 2986
BstBI TTCGAA 1 cut(s) 3036
BstC8I GCNNGC 3 cut(s) 310, 1430, 2307
BstEII GGTNACC 1 cut(s) 2275
BstENI CCTNNNNNAGG 1 cut(s) 163
BstMAI GTCTC 8 cut(s) 342, 556, 1058, 1529, 1661, 1759, 1862, 2725
BstNI CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
BstNSI RCATGY 1 cut(s) 2001
BstPAI GACNNNNGTC 1 cut(s) 2799
BstPI GGTNACC 1 cut(s) 2275
BstSCI CCNGG 7 cut(s) 355, 981, 1924, 2600, 2787, 2944, 3070
BstSFI CTRYAG 4 cut(s) 527, 666, 1044, 2899
BstSLI GKGCMC 1 cut(s) 383
BstV2I GAAGAC 2 cut(s) 209, 2223
BstX2I RGATCY 6 cut(s) 811, 1117, 1154, 1744, 3019, 3074
BstYI RGATCY 6 cut(s) 811, 1117, 1154, 1744, 3019, 3074
Bsu36I CCTNAGG 1 cut(s) 273
BsuI GTATCC 2 cut(s) 1220, 1276
BsuRI GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
BtsI GCAGTG 3 cut(s) 255, 2103, 2358
BtsIMutI CAGTG 6 cut(s) 255, 1910, 2103, 2358, 2908, 2920
BveI ACCTGC 1 cut(s) 2249
Cac8I GCNNGC 3 cut(s) 310, 1430, 2307
CaiI CAGNNNCTG 1 cut(s) 3077
Cfr10I RCCGGY 1 cut(s) 1434
Cfr13I GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
CseI GACGC 3 cut(s) 464, 695, 1214
CsiI ACCWGGT 1 cut(s) 2944
Csp6I GTAC 6 cut(s) 215, 399, 917, 1075, 1416, 2254
CviAII CATG 7 cut(s) 332, 537, 921, 1233, 1998, 2554, 2828
CviQI GTAC 6 cut(s) 215, 399, 917, 1075, 1416, 2254
DraI TTTAAA 1 cut(s) 442
DraIII CACNNNGTG 1 cut(s) 1310
EaeI YGGCCR 3 cut(s) 172, 363, 1786
Eam1104I CTCTTC 3 cut(s) 600, 929, 1117
EarI CTCTTC 3 cut(s) 600, 929, 1117
Eco130I CCWWGG 4 cut(s) 723, 1400, 2705, 2991
Eco147I AGGCCT 1 cut(s) 1042
Eco24I GRGCYC 2 cut(s) 383, 1446
Eco32I GATATC 1 cut(s) 1108
Eco47I GGWCC 4 cut(s) 8, 1239, 1958, 1989
Eco57I CTGAAG 7 cut(s) 249, 601, 1098, 1170, 1893, 2049, 2217
Eco81I CCTNAGG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 2111
Eco91I GGTNACC 1 cut(s) 2275
EcoNI CCTNNNNNAGG 1 cut(s) 163
EcoO109I RGGNCCY 3 cut(s) 379, 380, 1989
EcoO65I GGTNACC 1 cut(s) 2275
EcoRI GAATTC 1 cut(s) 2557
EcoRII CCWGG 5 cut(s) 355, 981, 1924, 2944, 3070
EcoRV GATATC 1 cut(s) 1108
EcoT14I CCWWGG 4 cut(s) 723, 1400, 2705, 2991
EcoT22I ATGCAT 1 cut(s) 1735
EcoT38I GRGCYC 2 cut(s) 383, 1446
ErhI CCWWGG 4 cut(s) 723, 1400, 2705, 2991
FaeI CATG 7 cut(s) 335, 540, 924, 1236, 2001, 2557, 2831
FalI AAGNNNNNCTT 4 cut(s) 630, 662, 1326, 1358
FaqI GGGAC 1 cut(s) 503
FatI CATG 7 cut(s) 331, 536, 920, 1232, 1997, 2553, 2827
FauNDI CATATG 1 cut(s) 2649
FriOI GRGCYC 2 cut(s) 383, 1446
FspBI CTAG 4 cut(s) 494, 1469, 2211, 2288
GsaI CCCAGC 2 cut(s) 1530, 2872
GsuI CTGGAG 2 cut(s) 2112, 2738
HaeIII GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
HapII CCGG 6 cut(s) 1435, 1593, 1961, 2602, 2789, 2931
HgaI GACGC 3 cut(s) 464, 695, 1214
Hin1II CATG 7 cut(s) 335, 540, 924, 1236, 2001, 2557, 2831
HincII GTYRAC 1 cut(s) 2392
HindII GTYRAC 1 cut(s) 2392
HindIII AAGCTT 1 cut(s) 1053
HpaII CCGG 6 cut(s) 1435, 1593, 1961, 2602, 2789, 2931
HphI GGTGA 8 cut(s) 653, 1214, 1479, 1525, 1650, 2035, 2213, 2945
Hpy166II GTNNAC 5 cut(s) 11, 1416, 2256, 2392, 2847
Hpy8I GTNNAC 5 cut(s) 11, 1416, 2256, 2392, 2847
HpyCH4III ACNGT 9 cut(s) 244, 424, 465, 1074, 1413, 1855, 1914, 2137, 2903
HpyCH4IV ACGT 3 cut(s) 2546, 2682, 2985
HpySE526I ACGT 3 cut(s) 2546, 2682, 2985
Hsp92II CATG 7 cut(s) 335, 540, 924, 1236, 2001, 2557, 2831
Kpn2I TCCGGA 1 cut(s) 1592
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 5 cut(s) 233, 1090, 1136, 2093, 2302
LweI GCATC 8 cut(s) 1226, 1555, 1678, 2096, 2205, 2370, 2527, 2675
MabI ACCWGGT 1 cut(s) 2944
MaeI CTAG 4 cut(s) 494, 1469, 2211, 2288
MaeII ACGT 3 cut(s) 2546, 2682, 2985
MfeI CAATTG 1 cut(s) 2863
MflI RGATCY 6 cut(s) 811, 1117, 1154, 1744, 3019, 3074
MhlI GDGCHC 3 cut(s) 383, 1446, 1658
MlsI TGGCCA 2 cut(s) 365, 1788
MluNI TGGCCA 2 cut(s) 365, 1788
MlyI GAGTC 8 cut(s) 32, 344, 740, 866, 875, 1493, 2123, 2494
MmeI TCCRAC 4 cut(s) 1083, 1920, 2400, 2465
Mox20I TGGCCA 2 cut(s) 365, 1788
Mph1103I ATGCAT 1 cut(s) 1735
MroI TCCGGA 1 cut(s) 1592
MroXI GAANNNNTTC 1 cut(s) 894
MscI TGGCCA 2 cut(s) 365, 1788
Msp20I TGGCCA 2 cut(s) 365, 1788
MspA1I CMGCKG 3 cut(s) 473, 1349, 2198
MspI CCGG 6 cut(s) 1435, 1593, 1961, 2602, 2789, 2931
MspR9I CCNGG 7 cut(s) 357, 983, 1926, 2602, 2789, 2946, 3072
MunI CAATTG 1 cut(s) 2863
Mva1269I GAATGC 2 cut(s) 194, 1912
MvaI CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
NciI CCSGG 2 cut(s) 2602, 2789
NdeI CATATG 1 cut(s) 2649
NlaIII CATG 7 cut(s) 335, 540, 924, 1236, 2001, 2557, 2831
NlaIV GGNNCC 5 cut(s) 235, 381, 436, 1990, 2929
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 5 cut(s) 611, 1202, 1485, 1513, 2023
NsiI ATGCAT 1 cut(s) 1735
NspI RCATGY 1 cut(s) 2001
NspV TTCGAA 1 cut(s) 3036
PaeR7I CTCGAG 1 cut(s) 2111
PceI AGGCCT 1 cut(s) 1042
PciSI GCTCTTC 1 cut(s) 600
PctI GAATGC 2 cut(s) 194, 1912
PdmI GAANNNNTTC 1 cut(s) 894
PflMI CCANNNNNTGG 1 cut(s) 1985
PfoI TCCNGGA 1 cut(s) 981
PleI GAGTC 8 cut(s) 31, 343, 739, 865, 875, 1492, 2122, 2493
PpsI GAGTC 8 cut(s) 31, 343, 739, 865, 875, 1492, 2122, 2493
Ppu21I YACGTR 1 cut(s) 2986
PpuMI RGGWCCY 1 cut(s) 1989
PshAI GACNNNNGTC 1 cut(s) 2799
PshBI ATTAAT 2 cut(s) 501, 717
Psp5II RGGWCCY 1 cut(s) 1989
Psp6I CCWGG 5 cut(s) 355, 981, 1924, 2944, 3070
PspEI GGTNACC 1 cut(s) 2275
PspFI CCCAGC 2 cut(s) 1526, 2868
PspGI CCWGG 5 cut(s) 355, 981, 1924, 2944, 3070
PspN4I GGNNCC 5 cut(s) 235, 381, 436, 1990, 2929
PspOMI GGGCCC 1 cut(s) 379
PspPI GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
PspPPI RGGWCCY 1 cut(s) 1989
PstI CTGCAG 2 cut(s) 531, 670
PstNI CAGNNNCTG 1 cut(s) 3077
PsuI RGATCY 6 cut(s) 811, 1117, 1154, 1744, 3019, 3074
PvuII CAGCTG 2 cut(s) 1349, 2198
RsaI GTAC 6 cut(s) 216, 400, 918, 1076, 1417, 2255
RsaNI GTAC 6 cut(s) 215, 399, 917, 1075, 1416, 2254
SapI GCTCTTC 1 cut(s) 600
Sau96I GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
SchI GAGTC 8 cut(s) 32, 344, 740, 866, 875, 1493, 2123, 2494
ScrFI CCNGG 7 cut(s) 357, 983, 1926, 2602, 2789, 2946, 3072
SduI GDGCHC 3 cut(s) 383, 1446, 1658
SexAI ACCWGGT 1 cut(s) 2944
SfaNI GCATC 8 cut(s) 1226, 1555, 1678, 2096, 2205, 2370, 2527, 2675
SfcI CTRYAG 4 cut(s) 527, 666, 1044, 2899
Sfr274I CTCGAG 1 cut(s) 2111
SfuI TTCGAA 1 cut(s) 3036
SinI GGWCC 4 cut(s) 8, 1239, 1958, 1989
SlaI CTCGAG 1 cut(s) 2111
SmlI CTYRAG 4 cut(s) 1036, 1439, 2111, 2175
SmoI CTYRAG 4 cut(s) 1036, 1439, 2111, 2175
SseBI AGGCCT 1 cut(s) 1042
SsiI CCGC 6 cut(s) 6, 237, 471, 1426, 2193, 2498
SspI AATATT 1 cut(s) 894
SspMI CTAG 4 cut(s) 494, 1469, 2211, 2288
StuI AGGCCT 1 cut(s) 1042
StyD4I CCNGG 7 cut(s) 355, 981, 1924, 2600, 2787, 2944, 3070
StyI CCWWGG 4 cut(s) 723, 1400, 2705, 2991
TaaI ACNGT 9 cut(s) 244, 424, 465, 1074, 1413, 1855, 1914, 2137, 2903
TaiI ACGT 3 cut(s) 2549, 2685, 2988
TaqI TCGA 5 cut(s) 1394, 1803, 2112, 2841, 3036
TatI WGTACW 3 cut(s) 1074, 1415, 2253
TauI GCSGC 5 cut(s) 239, 473, 1428, 2195, 2501
TscAI CASTG 6 cut(s) 255, 1917, 2103, 2358, 2908, 2920
TseFI GTSAC 5 cut(s) 611, 1202, 1485, 1513, 2023
Tsp45I GTSAC 5 cut(s) 611, 1202, 1485, 1513, 2023
TspDTI ATGAA 7 cut(s) 330, 587, 915, 1272, 1838, 2501, 2570
TspGWI ACGGA 1 cut(s) 1933
TspRI CASTG 6 cut(s) 255, 1917, 2103, 2358, 2908, 2920
Van91I CCANNNNNTGG 1 cut(s) 1985
VpaK11BI GGWCC 4 cut(s) 8, 1239, 1958, 1989
VspI ATTAAT 2 cut(s) 501, 717
XagI CCTNNNNNAGG 1 cut(s) 163
XapI RAATTY 7 cut(s) 657, 1314, 1935, 2530, 2557, 2590, 2816
XbaI TCTAGA 1 cut(s) 1468
XceI RCATGY 1 cut(s) 2001
XcmI CCANNNNNNNNNTGG 1 cut(s) 1196
XhoI CTCGAG 1 cut(s) 2111
XmnI GAANNNNTTC 1 cut(s) 894
XspI CTAG 4 cut(s) 494, 1469, 2211, 2288
Zsp2I ATGCAT 1 cut(s) 1735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.