pycom05g32400

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008511
Physical Location & Seq
Forward (+)
31808403 .. 31811148
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 774 bp
ATGGCGGCAGATCTGGTGCTCGACACAGCCATCAGAGACTGGGTTCTCATCCCTCTCTCGGTGGTCATGGTCCTCATCGGCGTCCTCCGATACTTCGTCTCCAAGCTCATGCGCTCCAACCAAGTCCCCGATGCCAAAATCGTCAAAGAAGGGTATTACACTTGCTTACTATACTACATGGATCTGCAATTAATTATTAGGGCTCGGAATTTACGTGCTGCTGCTAATTTCATACCTCCAAAGTCGTTTCGTGCTCGCCGATTGTATTATAACAATGAGGAAAATGGGCTGTTAATTGTTCCTAAGGGTCAGGCACAGAATCCACAAGCGCAAATGTTCTCTGATCCCAACATGGCCATGGATATGATGAAGAAAAATCTGTCCATGATAATACCCCAGACTCTTACTTTTGCATGGGTCAATTTTTTCTTCTCTGGATTTGTTGCAGCCAAAATTCCCTTTCCTCTTACTCAAAGGTTCAGGTCTATGCTGCAGAATGGGATTGACTTGAGCACTGTTGATGTTAGCTACGTCAGTAGCCGCTCATGGTACTTTCTTAATCTGTTTGGATTAAGAGGTTTGTTCAGTCTCATTTTGGGAGAAGAAAATGCCATGGATGATACTCAGAAAATGATGCAAATGAGTGGCTTTGGCTTTGATCCATCAAAGAGTTTGGGAGCCGAGAAGGACAGCCTCGATATAGTCCAGCATGACTGGGCCTTACCAAAATTTGAGCATCGCGCTGAAGCAGTATTGAGAAAACGTGCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF106

Protein Analysis

258

Amino Acids

29.46

Weight (kDa)

9.49

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC3_TMCO1 PF01956 4 - 203 4.8e-50 Integral membrane protein EMC3/TMCO1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 543
AccII CGCG 1 cut(s) 741
AciI CCGC 2 cut(s) 5, 541
AclWI GGATC 3 cut(s) 189, 338, 653
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 3 cut(s) 208, 453, 728
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 551
AfiI CCNNNNNNNGG 1 cut(s) 58
AluBI AGCT 2 cut(s) 106, 528
AluI AGCT 2 cut(s) 106, 528
Alw21I GWGCWC 3 cut(s) 21, 256, 515
Alw26I GTCTC 3 cut(s) 30, 103, 593
AlwI GGATC 3 cut(s) 189, 338, 653
AlwNI CAGNNNCTG 1 cut(s) 39
AoxI GGCC 2 cut(s) 354, 717
ApeKI GCWGC 4 cut(s) 218, 221, 446, 490
ApoI RAATTY 3 cut(s) 208, 453, 728
AseI ATTAAT 1 cut(s) 191
AspLEI GCGC 3 cut(s) 114, 331, 743
AspS9I GGNCC 2 cut(s) 70, 717
AvaII GGWCC 1 cut(s) 70
AxyI CCTNAGG 1 cut(s) 303
BalI TGGCCA 1 cut(s) 356
BanII GRGCYC 1 cut(s) 205
Bbv12I GWGCWC 3 cut(s) 21, 256, 515
BbvI GCAGC 4 cut(s) 205, 208, 458, 477
BccI CCATC 2 cut(s) 38, 670
BcoDI GTCTC 3 cut(s) 30, 103, 593
BfmI CTRYAG 1 cut(s) 491
BglII AGATCT 1 cut(s) 10
BisI GCNGC 6 cut(s) 6, 219, 222, 447, 491, 541
BlsI GCNGC 6 cut(s) 7, 220, 223, 448, 492, 542
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 70, 717
BmiI GGNNCC 1 cut(s) 679
BmrI ACTGGG 2 cut(s) 49, 724
BmsI GCATC 3 cut(s) 121, 624, 745
BmuI ACTGGG 2 cut(s) 49, 724
BpuEI CTTGAG 1 cut(s) 529
BsaAI YACGTR 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 357, 612
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 44, 719
Bse21I CCTNAGG 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 357, 612
BseGI GGATG 2 cut(s) 48, 622
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMII CTCAG 1 cut(s) 638
BseNI ACTGG 2 cut(s) 44, 719
BseXI GCAGC 4 cut(s) 205, 208, 458, 477
Bsh1236I CGCG 1 cut(s) 741
BshFI GGCC 2 cut(s) 356, 719
BsiHKAI GWGCWC 3 cut(s) 21, 256, 515
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 3 cut(s) 30, 103, 593
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 2 cut(s) 356, 719
Bsp1286I GDGCHC 4 cut(s) 21, 205, 256, 515
Bsp143I GATC 4 cut(s) 10, 181, 343, 658
Bsp19I CCATGG 2 cut(s) 357, 612
BspACI CCGC 2 cut(s) 5, 541
BspANI GGCC 2 cut(s) 356, 719
BspCNI CTCAG 1 cut(s) 637
BspFNI CGCG 1 cut(s) 741
BspLI GGNNCC 1 cut(s) 679
BspMAI CTGCAG 1 cut(s) 495
BspPI GGATC 3 cut(s) 189, 338, 653
BsrBI CCGCTC 1 cut(s) 543
BsrI ACTGG 2 cut(s) 44, 719
BssECI CCNNGG 2 cut(s) 357, 612
BssMI GATC 4 cut(s) 10, 181, 343, 658
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 2 cut(s) 357, 612
Bst4CI ACNGT 1 cut(s) 517
BstACI GRCGYC 1 cut(s) 81
BstBAI YACGTR 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 2 cut(s) 303, 624
BstDSI CCRYGG 2 cut(s) 357, 612
BstF5I GGATG 2 cut(s) 48, 622
BstFNI CGCG 1 cut(s) 741
BstHHI GCGC 3 cut(s) 114, 331, 743
BstKTI GATC 4 cut(s) 13, 184, 346, 661
BstMAI GTCTC 3 cut(s) 30, 103, 593
BstMBI GATC 4 cut(s) 10, 181, 343, 658
BstSFI CTRYAG 1 cut(s) 491
BstUI CGCG 1 cut(s) 741
BstV1I GCAGC 4 cut(s) 205, 208, 458, 477
BstX2I RGATCY 2 cut(s) 10, 181
BstYI RGATCY 2 cut(s) 10, 181
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 2 cut(s) 356, 719
BtgI CCRYGG 2 cut(s) 357, 612
BtgZI GCGATG 1 cut(s) 722
BtsCI GGATG 2 cut(s) 48, 622
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 1 cut(s) 256
CaiI CAGNNNCTG 1 cut(s) 39
CfoI GCGC 3 cut(s) 114, 331, 743
Cfr13I GGNCC 2 cut(s) 70, 717
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 550
CviQI GTAC 1 cut(s) 550
DdeI CTNAG 2 cut(s) 303, 624
DpnI GATC 4 cut(s) 12, 183, 345, 660
DpnII GATC 4 cut(s) 10, 181, 343, 658
EaeI YGGCCR 1 cut(s) 354
Eco130I CCWWGG 2 cut(s) 357, 612
Eco24I GRGCYC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 765
Eco81I CCTNAGG 1 cut(s) 303
EcoT14I CCWWGG 2 cut(s) 357, 612
EcoT38I GRGCYC 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 357, 612
Esp3I CGTCTC 1 cut(s) 103
FaqI GGGAC 1 cut(s) 110
Fnu4HI GCNGC 6 cut(s) 6, 219, 222, 447, 491, 541
FokI GGATG 2 cut(s) 35, 629
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 6 cut(s) 6, 219, 222, 447, 491, 541
GlaI GCGC 3 cut(s) 113, 330, 742
GluI GCNGC 6 cut(s) 6, 219, 222, 447, 491, 541
HaeIII GGCC 2 cut(s) 356, 719
HgaI GACGC 1 cut(s) 70
HhaI GCGC 3 cut(s) 114, 331, 743
Hin1I GRCGYC 1 cut(s) 81
Hin6I GCGC 3 cut(s) 112, 329, 741
HinP1I GCGC 3 cut(s) 112, 329, 741
HinfI GANTC 2 cut(s) 319, 400
Hpy188I TCNGA 5 cut(s) 35, 89, 207, 343, 627
Hpy188III TCNNGA 1 cut(s) 435
HpyAV CCTTC 2 cut(s) 143, 679
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4IV ACGT 3 cut(s) 214, 531, 763
HpyCH4V TGCA 5 cut(s) 187, 413, 446, 493, 637
HpyF3I CTNAG 2 cut(s) 303, 624
HpySE526I ACGT 3 cut(s) 214, 531, 763
Hsp92I GRCGYC 1 cut(s) 81
HspAI GCGC 3 cut(s) 112, 329, 741
Kzo9I GATC 4 cut(s) 10, 181, 343, 658
LmnI GCTCC 2 cut(s) 119, 677
LpnPI CCDG 7 cut(s) 25, 296, 410, 420, 466, 700, 719
Lsp1109I GCAGC 4 cut(s) 205, 208, 458, 477
LweI GCATC 3 cut(s) 121, 624, 745
MaeII ACGT 3 cut(s) 214, 531, 763
MalI GATC 4 cut(s) 12, 183, 345, 660
MbiI CCGCTC 1 cut(s) 543
MboI GATC 4 cut(s) 10, 181, 343, 658
MboII GAAGA 3 cut(s) 382, 421, 614
MflI RGATCY 2 cut(s) 10, 181
MhlI GDGCHC 4 cut(s) 21, 205, 256, 515
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 8 cut(s) 188, 192, 208, 226, 294, 421, 453, 728
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 8 cut(s) 63, 83, 95, 246, 271, 474, 569, 704
Mox20I TGGCCA 1 cut(s) 356
MscI TGGCCA 1 cut(s) 356
MseI TTAA 4 cut(s) 191, 293, 558, 572
MslI CAYNNNNRTG 2 cut(s) 356, 362
Msp20I TGGCCA 1 cut(s) 356
MvnI CGCG 1 cut(s) 741
NcoI CCATGG 2 cut(s) 357, 612
NdeII GATC 4 cut(s) 10, 181, 343, 658
NlaIV GGNNCC 1 cut(s) 679
NmeAIII GCCGAG 1 cut(s) 706
PcsI WCGNNNNNNNCGW 2 cut(s) 211, 256
PfeI GAWTC 1 cut(s) 319
PkrI GCNGC 6 cut(s) 7, 220, 223, 448, 492, 542
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
Ppu21I YACGTR 1 cut(s) 215
PshBI ATTAAT 1 cut(s) 191
PsiI TTATAA 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 679
PspPI GGNCC 2 cut(s) 70, 717
PstI CTGCAG 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 39
PsuI RGATCY 2 cut(s) 10, 181
RsaI GTAC 1 cut(s) 551
RsaNI GTAC 1 cut(s) 550
RseI CAYNNNNRTG 2 cut(s) 356, 362
SaqAI TTAA 4 cut(s) 191, 293, 558, 572
SatI GCNGC 6 cut(s) 6, 219, 222, 447, 491, 541
Sau3AI GATC 4 cut(s) 10, 181, 343, 658
Sau96I GGNCC 2 cut(s) 70, 717
SchI GAGTC 1 cut(s) 394
SduI GDGCHC 4 cut(s) 21, 205, 256, 515
SetI ASST 9 cut(s) 108, 217, 238, 479, 485, 530, 534, 580, 766
SfaNI GCATC 3 cut(s) 121, 624, 745
SfcI CTRYAG 1 cut(s) 491
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 2 cut(s) 356, 362
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
Sse9I AATT 8 cut(s) 188, 192, 208, 226, 294, 421, 453, 728
SsiI CCGC 2 cut(s) 5, 541
StyI CCWWGG 2 cut(s) 357, 612
TaaI ACNGT 1 cut(s) 517
TaiI ACGT 3 cut(s) 217, 534, 766
TaqI TCGA 2 cut(s) 21, 696
TasI AATT 8 cut(s) 188, 192, 208, 226, 294, 421, 453, 728
TauI GCSGC 2 cut(s) 8, 543
TfiI GAWTC 1 cut(s) 319
Tru1I TTAA 4 cut(s) 191, 293, 558, 572
Tru9I TTAA 4 cut(s) 191, 293, 558, 572
TscAI CASTG 1 cut(s) 520
TseI GCWGC 4 cut(s) 218, 221, 446, 490
TspDTI ATGAA 2 cut(s) 220, 383
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 70
VspI ATTAAT 1 cut(s) 191
XapI RAATTY 3 cut(s) 208, 453, 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.