pycom06g03030

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007990
Physical Location & Seq
Forward (+)
3819056 .. 3821972
2917 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1617 bp
ATGGGTAGGAGGAAACCCAAGGACCCAGAAACCGACGCCGTTTCAGCAGCTCAGTCCGACGTCTTCAAGACCCTATTCGGCGACGCCACCAGCCACGACGCCGCCCCTGCCATCTTCTCCGAGAGCAACCCCTTCAGGAGGAAGAACCAGGAATCTGGGTTTGGGTTTGTTGCCCCTTCTCAACCTGCTGAGAACACCAATAATGGCGAGTCTGAGAACAATGGCGATGACGGGGACGGCGAGGTGCAGAAACGGAAGCGGAAGAACAAAGAAAAGGCGGCTTCCGGGTTGATTAACGAAGAAGCTCCACAGGGTCCTGTGGAGTTGGAGGGCGAAAAACCAAAGAAAAATAAACTGCCGGAAACGCTCAATTCTGAAAACCCTAATTTGGGCTCTGAGTTAGAAGGGGTGTCAAGACAGGAAAATGATGAACCCTCCGAGACGACGAATGCGGTGGCGAAGAAGAAGGATGGGAGGAAGAGGAAAAGGGATGAAGTTGAGAGAGAATATGAGGTGAAGAAGTACGGCGTGAAGGAGGGCGAGGAGGATGGGGACAAGGAGAAGAGGACTGTTGGAGAGAAGAGGAAGACAGTTGATAATCCAGCTGATATGTTGGTTTCCAACGAAGGTTTCGACGACGAGAGTAAGCTCTTGAGGACTGTTTTTGTTGGAAATTTGCCCTTGAAGGTCAAGAAAAAAGCTTTGATGAAGGAGTTCACTAAATTTGGAGAGGTGGAGTCTGTGAGGATTCGATCCGTGCCAATTTTGGATACCAAAAGGCCCAGAAAGGGTGCAATACTCACAAAACAAATCCATGATAAAGCTGACAGCGTTAATGCCTACATTGTTTTCAAAACCGAGGAATCGGCACAGGCTTCTTTGTCCCATAACATGGCTGTGGTTGAAGGACATCATATCCGTGTTGACAGGGCTTGCCCACCTCGTAAGAAGCTGAAAGGGGAGAGTGCTACTGTTTATGATCACACGAGAACAGTTTTTGTGGGTAACCTTCCATTTGATGTAAAGGATGAAGAAGTTTATCAGTTGTTTTGTGGTATCAACAATCTTGGATCCAGCGTTGAAGCTATTCGCATAATCAGGGATCCTAATTATGGTATCGGAAAGGGCATTGCTTATGTTTTATTTAGAACAAGGGAAACTGCAAATTTGGTCGTTAAGAAGCGAAACTTGAAGCTTGGGGATCGGGAGCTCAGGCTGTCTCATGCAAAACCAGAGTCCACCCCAACTAAGAGAAAGAACCCCTCATCAGCATCAGAAGCTAATTCTTCAGCCAAAAAGCGAGCTGTGGACTCGAGGAGTCCAGATTTTAACAAGTCGAGTTCAAGGGCATCTTACCAGGGCCTGCGTGCAGGTAAGCCTGGTGTCCAAAAGAAGTTCAATGCAAAAGGCAATAGACCAGATAAGTTTGAATCAAAATCACCAAGTGGAGTGAAGCCGAAAGAACGGAAAGATAAACGACCATCTGTTGCTGCGAGAAAGGCTAAAGAAGCACTTAAAGGTGGCGCTGCTTCAAAACAAACAGGTACAAAGCGTAAGCTTGATAGCCGGACTCCAGATAGCTCCCAGAAGAAAAAGAAATTCATGAAATATAGGTAG

Protein Analysis

539

Amino Acids

59.75

Weight (kDa)

9.77

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 332 - 405 4.2e-09 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
Acc36I ACCTGC 2 cut(s) 193, 1361
AccB7I CCANNNNNTGG 1 cut(s) 892
AciI CCGC 4 cut(s) 102, 259, 278, 452
AclWI GGATC 6 cut(s) 747, 1065, 1078, 1097, 1110, 1209
AcsI RAATTY 4 cut(s) 673, 722, 1165, 1598
AcuI CTGAAG 2 cut(s) 118, 1272
AcyI GRCGYC 4 cut(s) 36, 60, 84, 99
AdeI CACNNNGTG 1 cut(s) 1445
AfaI GTAC 2 cut(s) 524, 1546
AfiI CCNNNNNNNGG 6 cut(s) 138, 388, 389, 788, 892, 1112
AjnI CCWGG 3 cut(s) 147, 1356, 1378
AjuI GAANNNNNNNTTGG 4 cut(s) 144, 176, 614, 646
Alw21I GWGCWC 1 cut(s) 1212
Alw26I GTCTC 2 cut(s) 434, 1224
AlwI GGATC 6 cut(s) 747, 1065, 1078, 1097, 1110, 1209
AlwNI CAGNNNCTG 1 cut(s) 1363
Ama87I CYCGRG 1 cut(s) 1312
AoxI GGCC 2 cut(s) 779, 1360
ApeKI GCWGC 3 cut(s) 47, 1490, 1526
ApoI RAATTY 4 cut(s) 673, 722, 1165, 1598
ArsI GACNNNNNNTTYG 2 cut(s) 1220, 1252
Asp700I GAANNNNTTC 2 cut(s) 713, 1086
AspLEI GCGC 1 cut(s) 1526
AspS9I GGNCC 4 cut(s) 22, 314, 780, 1360
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 2 cut(s) 526, 1431
AvaI CYCGRG 1 cut(s) 1312
AvaII GGWCC 2 cut(s) 22, 314
BamHI GGATCC 2 cut(s) 1070, 1102
BanII GRGCYC 2 cut(s) 395, 1212
BauI CACGAG 1 cut(s) 985
BbsI GAAGAC 2 cut(s) 55, 593
Bbv12I GWGCWC 1 cut(s) 1212
BbvI GCAGC 3 cut(s) 59, 1477, 1513
BccI CCATC 4 cut(s) 119, 464, 542, 1489
BceAI ACGGC 3 cut(s) 23, 253, 541
BciT130I CCWGG 3 cut(s) 149, 1358, 1380
BciVI GTATCC 1 cut(s) 763
BclI TGATCA 1 cut(s) 979
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 2 cut(s) 434, 1224
BfoI RGCGCY 1 cut(s) 1527
BfuAI ACCTGC 2 cut(s) 193, 1361
BfuI GTATCC 1 cut(s) 763
BisI GCNGC 5 cut(s) 48, 102, 279, 1491, 1527
BlsI GCNGC 5 cut(s) 49, 103, 280, 1492, 1528
Bme1390I CCNGG 4 cut(s) 149, 286, 1358, 1380
Bme18I GGWCC 2 cut(s) 22, 314
BmeT110I CYCGRG 1 cut(s) 1312
BmgT120I GGNCC 4 cut(s) 22, 314, 780, 1360
BmiI GGNNCC 4 cut(s) 24, 315, 1072, 1104
BmrFI CCNGG 4 cut(s) 149, 286, 1358, 1380
BmsI GCATC 2 cut(s) 1280, 1358
BpiI GAAGAC 2 cut(s) 55, 593
BpmI CTGGAG 1 cut(s) 1557
Bpu10I CCTNAGC 1 cut(s) 1211
BpuEI CTTGAG 1 cut(s) 673
BpuMI CCSGG 1 cut(s) 286
BsaHI GRCGYC 4 cut(s) 36, 60, 84, 99
BsaJI CCNNGG 3 cut(s) 18, 858, 1357
Bsc4I CCNNNNNNNGG 6 cut(s) 138, 388, 389, 788, 892, 1112
Bse3DI GCAATG 1 cut(s) 1128
BseBI CCWGG 3 cut(s) 149, 1358, 1380
BseDI CCNNGG 3 cut(s) 18, 858, 1357
BseGI GGATG 4 cut(s) 475, 496, 553, 1033
BseLI CCNNNNNNNGG 6 cut(s) 138, 388, 389, 788, 892, 1112
BseMI GCAATG 1 cut(s) 1128
BseMII CTCAG 5 cut(s) 65, 180, 204, 387, 1225
BseRI GAGGAG 2 cut(s) 557, 1330
BseXI GCAGC 3 cut(s) 59, 1477, 1513
BsgI GTGCAG 2 cut(s) 266, 1389
BshFI GGCC 2 cut(s) 781, 1362
BsiHKAI GWGCWC 1 cut(s) 1212
BsiHKCI CYCGRG 1 cut(s) 1312
BsiSI CCGG 3 cut(s) 285, 359, 1567
BslFI GGGAC 3 cut(s) 248, 566, 868
BslI CCNNNNNNNGG 6 cut(s) 138, 388, 389, 788, 892, 1112
BsmAI GTCTC 2 cut(s) 434, 1224
BsmBI CGTCTC 1 cut(s) 434
BsmFI GGGAC 3 cut(s) 248, 566, 868
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 2 cut(s) 781, 1362
BsoBI CYCGRG 1 cut(s) 1312
Bsp1286I GDGCHC 2 cut(s) 395, 1212
Bsp143I GATC 5 cut(s) 752, 979, 1070, 1102, 1201
BspACI CCGC 4 cut(s) 102, 259, 278, 452
BspANI GGCC 2 cut(s) 781, 1362
BspCNI CTCAG 5 cut(s) 64, 181, 205, 388, 1224
BspHI TCATGA 1 cut(s) 1602
BspLI GGNNCC 4 cut(s) 24, 315, 1072, 1104
BspMI ACCTGC 2 cut(s) 193, 1361
BspPI GGATC 6 cut(s) 747, 1065, 1078, 1097, 1110, 1209
BsrDI GCAATG 1 cut(s) 1128
BssECI CCNNGG 3 cut(s) 18, 858, 1357
BssMI GATC 5 cut(s) 752, 979, 1070, 1102, 1201
BssNI GRCGYC 4 cut(s) 36, 60, 84, 99
BssSI CACGAG 1 cut(s) 985
BssT1I CCWWGG 1 cut(s) 18
Bst2BI CACGAG 1 cut(s) 985
Bst2UI CCWGG 3 cut(s) 149, 1358, 1380
Bst4CI ACNGT 5 cut(s) 571, 592, 661, 973, 994
Bst6I CTCTTC 3 cut(s) 473, 557, 575
BstACI GRCGYC 4 cut(s) 36, 60, 84, 99
BstC8I GCNNGC 4 cut(s) 934, 1302, 1364, 1368
BstDEI CTNAG 6 cut(s) 51, 189, 213, 396, 1211, 1248
BstEII GGTNACC 1 cut(s) 1004
BstENI CCTNNNNNAGG 1 cut(s) 136
BstF5I GGATG 4 cut(s) 475, 496, 553, 1033
BstH2I RGCGCY 1 cut(s) 1527
BstHHI GCGC 1 cut(s) 1526
BstKTI GATC 5 cut(s) 755, 982, 1073, 1105, 1204
BstMAI GTCTC 2 cut(s) 434, 1224
BstMBI GATC 5 cut(s) 752, 979, 1070, 1102, 1201
BstMWI GCNNNNNNNGC 6 cut(s) 44, 107, 364, 1277, 1499, 1508
BstNI CCWGG 3 cut(s) 149, 1358, 1380
BstPI GGTNACC 1 cut(s) 1004
BstSCI CCNGG 4 cut(s) 147, 284, 1356, 1378
BstV1I GCAGC 3 cut(s) 59, 1477, 1513
BstV2I GAAGAC 2 cut(s) 55, 593
BstX2I RGATCY 2 cut(s) 1070, 1102
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 2 cut(s) 1070, 1102
BsuI GTATCC 1 cut(s) 763
BsuRI GGCC 2 cut(s) 781, 1362
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 4 cut(s) 475, 496, 553, 1033
BveI ACCTGC 2 cut(s) 193, 1361
Cac8I GCNNGC 4 cut(s) 934, 1302, 1364, 1368
CaiI CAGNNNCTG 1 cut(s) 1363
CciI TCATGA 1 cut(s) 1602
CfoI GCGC 1 cut(s) 1526
Cfr13I GGNCC 4 cut(s) 22, 314, 780, 1360
CseI GACGC 3 cut(s) 44, 92, 107
Csp6I GTAC 2 cut(s) 523, 1545
CviAII CATG 4 cut(s) 815, 892, 1223, 1603
CviQI GTAC 2 cut(s) 523, 1545
DdeI CTNAG 6 cut(s) 51, 189, 213, 396, 1211, 1248
DpnI GATC 5 cut(s) 754, 981, 1072, 1104, 1203
DpnII GATC 5 cut(s) 752, 979, 1070, 1102, 1201
DraIII CACNNNGTG 1 cut(s) 1445
Eam1104I CTCTTC 3 cut(s) 473, 557, 575
EarI CTCTTC 3 cut(s) 473, 557, 575
Ecl136II GAGCTC 1 cut(s) 1210
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 395, 1212
Eco47I GGWCC 2 cut(s) 22, 314
Eco53kI GAGCTC 1 cut(s) 1210
Eco57I CTGAAG 2 cut(s) 118, 1272
Eco88I CYCGRG 1 cut(s) 1312
Eco91I GGTNACC 1 cut(s) 1004
EcoICRI GAGCTC 1 cut(s) 1210
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoO109I RGGNCCY 3 cut(s) 22, 314, 1360
EcoO65I GGTNACC 1 cut(s) 1004
EcoRII CCWGG 3 cut(s) 147, 1356, 1378
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 395, 1212
ErhI CCWWGG 1 cut(s) 18
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 4 cut(s) 818, 895, 1226, 1606
FalI AAGNNNNNCTT 6 cut(s) 1172, 1204, 1336, 1368, 1497, 1529
FaqI GGGAC 3 cut(s) 248, 566, 868
FatI CATG 4 cut(s) 814, 891, 1222, 1602
FbaI TGATCA 1 cut(s) 979
Fnu4HI GCNGC 5 cut(s) 48, 102, 279, 1491, 1527
FokI GGATG 4 cut(s) 482, 503, 560, 1040
FriOI GRGCYC 2 cut(s) 395, 1212
Fsp4HI GCNGC 5 cut(s) 48, 102, 279, 1491, 1527
GlaI GCGC 1 cut(s) 1525
GluI GCNGC 5 cut(s) 48, 102, 279, 1491, 1527
GsuI CTGGAG 1 cut(s) 1557
HaeII RGCGCY 1 cut(s) 1527
HaeIII GGCC 2 cut(s) 781, 1362
HapII CCGG 3 cut(s) 285, 359, 1567
HgaI GACGC 3 cut(s) 44, 92, 107
HhaI GCGC 1 cut(s) 1526
Hin1I GRCGYC 4 cut(s) 36, 60, 84, 99
Hin1II CATG 4 cut(s) 818, 895, 1226, 1606
Hin6I GCGC 1 cut(s) 1524
HinP1I GCGC 1 cut(s) 1524
HincII GTYRAC 1 cut(s) 925
HindII GTYRAC 1 cut(s) 925
HindIII AAGCTT 3 cut(s) 699, 1193, 1556
HpaII CCGG 3 cut(s) 285, 359, 1567
HphI GGTGA 2 cut(s) 526, 1431
Hpy166II GTNNAC 4 cut(s) 717, 925, 1239, 1309
Hpy188I TCNGA 8 cut(s) 58, 121, 214, 376, 397, 439, 1121, 1276
Hpy188III TCNNGA 9 cut(s) 67, 136, 414, 652, 691, 1205, 1322, 1574, 1603
Hpy8I GTNNAC 4 cut(s) 717, 925, 1239, 1309
Hpy99I CGWCG 7 cut(s) 38, 62, 86, 101, 448, 638, 641
HpyCH4III ACNGT 5 cut(s) 571, 592, 661, 973, 994
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 6 cut(s) 247, 794, 1163, 1226, 1370, 1403
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 107, 364, 1277, 1499, 1508
HpyF3I CTNAG 6 cut(s) 51, 189, 213, 396, 1211, 1248
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 4 cut(s) 36, 60, 84, 99
Hsp92II CATG 4 cut(s) 818, 895, 1226, 1606
HspAI GCGC 1 cut(s) 1524
Ksp22I TGATCA 1 cut(s) 979
Kzo9I GATC 5 cut(s) 752, 979, 1070, 1102, 1201
LmnI GCTCC 3 cut(s) 310, 1207, 1586
Lsp1109I GCAGC 3 cut(s) 59, 1477, 1513
LweI GCATC 2 cut(s) 1280, 1358
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 1004
MalI GATC 5 cut(s) 754, 981, 1072, 1104, 1203
MboI GATC 5 cut(s) 752, 979, 1070, 1102, 1201
MflI RGATCY 2 cut(s) 1070, 1102
MhlI GDGCHC 2 cut(s) 395, 1212
MluCI AATT 9 cut(s) 370, 385, 673, 722, 762, 1108, 1165, 1282, 1598
MlyI GAGTC 6 cut(s) 218, 746, 1244, 1304, 1327, 1564
MmeI TCCRAC 5 cut(s) 81, 306, 553, 645, 649
MroXI GAANNNNTTC 2 cut(s) 713, 1086
MseI TTAA 5 cut(s) 294, 834, 1176, 1329, 1515
MslI CAYNNNNRTG 2 cut(s) 896, 918
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 3 cut(s) 285, 359, 1567
MspR9I CCNGG 4 cut(s) 149, 286, 1358, 1380
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 3 cut(s) 149, 1358, 1380
MwoI GCNNNNNNNGC 6 cut(s) 44, 107, 364, 1277, 1499, 1508
NciI CCSGG 1 cut(s) 286
NdeII GATC 5 cut(s) 752, 979, 1070, 1102, 1201
NlaIII CATG 4 cut(s) 818, 895, 1226, 1606
NlaIV GGNNCC 4 cut(s) 24, 315, 1072, 1104
PaeR7I CTCGAG 1 cut(s) 1312
PagI TCATGA 1 cut(s) 1602
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 630
PctI GAATGC 1 cut(s) 454
PdmI GAANNNNTTC 2 cut(s) 713, 1086
PfeI GAWTC 4 cut(s) 152, 748, 863, 1430
PflMI CCANNNNNTGG 1 cut(s) 892
PkrI GCNGC 5 cut(s) 49, 103, 280, 1492, 1528
PleI GAGTC 6 cut(s) 217, 745, 1243, 1304, 1326, 1564
PpsI GAGTC 6 cut(s) 217, 745, 1243, 1304, 1326, 1564
PpuMI RGGWCCY 2 cut(s) 22, 314
Psp124BI GAGCTC 1 cut(s) 1212
Psp5II RGGWCCY 2 cut(s) 22, 314
Psp6I CCWGG 3 cut(s) 147, 1356, 1378
PspEI GGTNACC 1 cut(s) 1004
PspGI CCWGG 3 cut(s) 147, 1356, 1378
PspN4I GGNNCC 4 cut(s) 24, 315, 1072, 1104
PspPI GGNCC 4 cut(s) 22, 314, 780, 1360
PspPPI RGGWCCY 2 cut(s) 22, 314
PspXI VCTCGAGB 1 cut(s) 1312
PstNI CAGNNNCTG 1 cut(s) 1363
PsuI RGATCY 2 cut(s) 1070, 1102
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 2 cut(s) 524, 1546
RsaNI GTAC 2 cut(s) 523, 1545
RseI CAYNNNNRTG 2 cut(s) 896, 918
SacI GAGCTC 1 cut(s) 1212
SaqAI TTAA 5 cut(s) 294, 834, 1176, 1329, 1515
SatI GCNGC 5 cut(s) 48, 102, 279, 1491, 1527
Sau3AI GATC 5 cut(s) 752, 979, 1070, 1102, 1201
Sau96I GGNCC 4 cut(s) 22, 314, 780, 1360
SchI GAGTC 6 cut(s) 218, 746, 1244, 1304, 1327, 1564
ScrFI CCNGG 4 cut(s) 149, 286, 1358, 1380
SduI GDGCHC 2 cut(s) 395, 1212
SfaNI GCATC 2 cut(s) 1280, 1358
Sfr274I CTCGAG 1 cut(s) 1312
SinI GGWCC 2 cut(s) 22, 314
SlaI CTCGAG 1 cut(s) 1312
SmiMI CAYNNNNRTG 2 cut(s) 896, 918
SmlI CTYRAG 2 cut(s) 652, 1312
SmoI CTYRAG 2 cut(s) 652, 1312
Sse9I AATT 9 cut(s) 370, 385, 673, 722, 762, 1108, 1165, 1282, 1598
SsiI CCGC 4 cut(s) 102, 259, 278, 452
SstI GAGCTC 1 cut(s) 1212
StyD4I CCNGG 4 cut(s) 147, 284, 1356, 1378
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 5 cut(s) 571, 592, 661, 973, 994
TaiI ACGT 1 cut(s) 63
TaqI TCGA 4 cut(s) 633, 751, 1313, 1337
TasI AATT 9 cut(s) 370, 385, 673, 722, 762, 1108, 1165, 1282, 1598
TauI GCSGC 2 cut(s) 104, 281
TfiI GAWTC 4 cut(s) 152, 748, 863, 1430
Tru1I TTAA 5 cut(s) 294, 834, 1176, 1329, 1515
Tru9I TTAA 5 cut(s) 294, 834, 1176, 1329, 1515
TseI GCWGC 3 cut(s) 47, 1490, 1526
TspDTI ATGAA 5 cut(s) 444, 507, 722, 1044, 1591
TspGWI ACGGA 4 cut(s) 268, 745, 908, 1480
Van91I CCANNNNNTGG 1 cut(s) 892
VpaK11BI GGWCC 2 cut(s) 22, 314
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 4 cut(s) 673, 722, 1165, 1598
XhoI CTCGAG 1 cut(s) 1312
XmnI GAANNNNTTC 2 cut(s) 713, 1086
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.