pycom06g04730

Formyltetrahydrofolate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002503
Physical Location & Seq
Forward (+)
6293621 .. 6297256
3636 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 984 bp
ATGAACCTCGTCCAAAGGGCAGCTCCAAGCCTCCCCAAGCTTTTCGGATTTGCCAAGAGGCCCTTCAAGTCCTTGAGATTCCCAGGGGAGCCCCTCGACTCTTCATCTTCTCTCCCCCCTCTCTCCTACGGAATCCACGTCTTCCATTGCCCCGATGCAGTTGGGATTGTGGCCAAGCTATCGGATTGCATTGCTTCTAAAGGCGGAAACATTCTCGGCGCCAGCATCTTCGTGCCCGAAAACAAGCACGTTTTTTATTCCAGAAGCGAATTTATTTTCGATCCTCTTAAATGGCCAAGAAAGGAAATGGATGAGGATTTCCATAAGCTGTCGTTAACATACTCTGCAATGAGATCTGTTGTCCGGGTTCCAAATCTTGACCCCAAGTATAAGATTGGTGTCCTTGTCTCAAAACAGGATCATTGCCTGGTTGATTTATTACATCAGTGGCAGGAAGGCAGGCTTCCAGTAGATATAACTTGTGTAATAAGTAATCATCATCGAGGTCCGAACACCCATGTGCATCGTTTTCTAGAAAGACACGGTATACCTTACCATTATCTAAATACAACCATGGATGACAAAAGAGAAGGGGAGATACTAGAGTTGGTTCAAAACACTGATTTTTTAGTTCTTGCCAGGTACATGCAGATATTATCCGGCGATTTTTTAAAGAGCTATGGAAAGGATATAATTAACATTCATCATGGCCTTTTGCCGTCATTCAAAGGTGGAAAACCATCTAAACAGGCTTTTGATGCAGGGGTGAAGCTTATTGGTGCAACAACTCATTTTGTGACGCAAGAACTTGATGCTGGGCCTATTATTGAACAGATGGTTGCGAGAGTTTCACACAGAGATAACTTGCAGACTTTTGTGCAGAAATCGGAGAACCTCGAAAAACAATGCCTAACGATGGCTATCAAGTCATACTGTGAGCTGCGAGTATTACCTTACGAGGATAACAAGACTGTTGTATTTTGA

Protein Analysis

328

Amino Acids

37.05

Weight (kDa)

8.61

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Formyl_trans_N PF00551 131 - 307 2.5e-34 Formyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 547
AciI CCGC 1 cut(s) 204
AclWI GGATC 2 cut(s) 275, 426
AcoI YGGCCR 2 cut(s) 171, 293
AcsI RAATTY 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 1 cut(s) 644
AfiI CCNNNNNNNGG 1 cut(s) 916
AgsI TTSAA 4 cut(s) 67, 614, 727, 830
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 3 cut(s) 82, 426, 638
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
AleI CACNNNNGTG 1 cut(s) 518
AluBI AGCT 7 cut(s) 23, 40, 178, 328, 678, 772, 940
AluI AGCT 7 cut(s) 23, 40, 178, 328, 678, 772, 940
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 2 cut(s) 275, 426
AoxI GGCC 5 cut(s) 59, 171, 293, 709, 818
ApeKI GCWGC 2 cut(s) 20, 940
ApoI RAATTY 1 cut(s) 269
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 3 cut(s) 60, 506, 818
AsuC2I CCSGG 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 778
AvaII GGWCC 1 cut(s) 506
BaeGI GKGCMC 1 cut(s) 237
BaeI ACNNNNGTAYC 2 cut(s) 590, 623
BalI TGGCCA 2 cut(s) 173, 295
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 93
BarI GAAGNNNNNNTAC 2 cut(s) 582, 614
BbsI GAAGAC 1 cut(s) 133
BbvI GCAGC 2 cut(s) 32, 927
BccI CCATC 3 cut(s) 748, 829, 910
BceAI ACGGC 1 cut(s) 703
BciT130I CCWGG 3 cut(s) 84, 428, 640
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 1 cut(s) 412
BfaI CTAG 2 cut(s) 533, 602
BfoI RGCGCY 1 cut(s) 222
BglII AGATCT 1 cut(s) 353
BisI GCNGC 2 cut(s) 21, 941
BlsI GCNGC 2 cut(s) 22, 942
Bme1390I CCNGG 4 cut(s) 84, 365, 428, 640
Bme18I GGWCC 1 cut(s) 506
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 3 cut(s) 60, 506, 818
BmiI GGNNCC 3 cut(s) 90, 220, 369
BmrFI CCNGG 4 cut(s) 84, 365, 428, 640
BmsI GCATC 5 cut(s) 145, 234, 532, 748, 802
BpiI GAAGAC 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 94
BpuMI CCSGG 1 cut(s) 365
BsaBI GATNNNNATC 1 cut(s) 920
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 82, 83, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 916
Bse1I ACTGG 1 cut(s) 467
Bse3DI GCAATG 4 cut(s) 145, 189, 354, 421
Bse8I GATNNNNATC 1 cut(s) 920
BseBI CCWGG 3 cut(s) 84, 428, 640
BseDI CCNNGG 3 cut(s) 82, 83, 573
BseGI GGATG 2 cut(s) 316, 583
BseJI GATNNNNATC 1 cut(s) 920
BseLI CCNNNNNNNGG 1 cut(s) 916
BseMI GCAATG 4 cut(s) 145, 189, 354, 421
BseNI ACTGG 1 cut(s) 467
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 2 cut(s) 32, 927
BseYI CCCAGC 1 cut(s) 815
BsgI GTGCAG 1 cut(s) 899
BshFI GGCC 5 cut(s) 61, 173, 295, 711, 820
BshNI GGYRCC 1 cut(s) 218
BsiSI CCGG 2 cut(s) 364, 660
BslI CCNNNNNNNGG 1 cut(s) 916
BsmAI GTCTC 1 cut(s) 412
BsnI GGCC 5 cut(s) 61, 173, 295, 711, 820
Bsp1286I GDGCHC 2 cut(s) 93, 237
Bsp143I GATC 3 cut(s) 280, 353, 418
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 1 cut(s) 204
BspANI GGCC 5 cut(s) 61, 173, 295, 711, 820
BspLI GGNNCC 3 cut(s) 90, 220, 369
BspPI GGATC 2 cut(s) 275, 426
BspT107I GGYRCC 1 cut(s) 218
BsrDI GCAATG 4 cut(s) 145, 189, 354, 421
BsrI ACTGG 1 cut(s) 467
BssECI CCNNGG 3 cut(s) 82, 83, 573
BssMI GATC 3 cut(s) 280, 353, 418
BssNAI GTATAC 1 cut(s) 548
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 573
Bst1107I GTATAC 1 cut(s) 548
Bst2UI CCWGG 3 cut(s) 84, 428, 640
Bst4CI ACNGT 3 cut(s) 545, 935, 973
Bst6I CTCTTC 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 223, 461
BstDSI CCRYGG 1 cut(s) 573
BstF5I GGATG 2 cut(s) 316, 583
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 3 cut(s) 283, 356, 421
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 3 cut(s) 280, 353, 418
BstMWI GCNNNNNNNGC 1 cut(s) 758
BstNI CCWGG 3 cut(s) 84, 428, 640
BstNSI RCATGY 1 cut(s) 649
BstSCI CCNGG 4 cut(s) 82, 363, 426, 638
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 32, 927
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BstZ17I GTATAC 1 cut(s) 548
BsuRI GGCC 5 cut(s) 61, 173, 295, 711, 820
BtgI CCRYGG 1 cut(s) 573
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 2 cut(s) 316, 583
BtsIMutI CAGTG 2 cut(s) 452, 618
Cac8I GCNNGC 2 cut(s) 223, 461
CfoI GCGC 1 cut(s) 221
Cfr13I GGNCC 3 cut(s) 60, 506, 818
CseI GACGC 1 cut(s) 808
Csp6I GTAC 1 cut(s) 643
CviAII CATG 4 cut(s) 518, 574, 646, 707
CviQI GTAC 1 cut(s) 643
DinI GGCGCC 1 cut(s) 220
DpnI GATC 3 cut(s) 282, 355, 420
DpnII GATC 3 cut(s) 280, 353, 418
DraI TTTAAA 1 cut(s) 672
EaeI YGGCCR 2 cut(s) 171, 293
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
EciI GGCGGA 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 573
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 60
EcoRII CCWGG 3 cut(s) 82, 426, 638
EcoT14I CCWWGG 1 cut(s) 573
EcoT38I GRGCYC 1 cut(s) 93
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 4 cut(s) 521, 577, 649, 710
FalI AAGNNNNNCTT 4 cut(s) 47, 79, 447, 479
FatI CATG 4 cut(s) 517, 573, 645, 706
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 21, 941
FokI GGATG 2 cut(s) 323, 590
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 2 cut(s) 21, 941
FspBI CTAG 2 cut(s) 533, 602
GlaI GCGC 1 cut(s) 220
GluI GCNGC 2 cut(s) 21, 941
GsaI CCCAGC 1 cut(s) 819
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 5 cut(s) 61, 173, 295, 711, 820
HapII CCGG 2 cut(s) 364, 660
HgaI GACGC 1 cut(s) 808
HhaI GCGC 1 cut(s) 221
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 521, 577, 649, 710
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 2 cut(s) 38, 770
HinfI GANTC 3 cut(s) 78, 98, 132
HpaI GTTAAC 1 cut(s) 336
HpaII CCGG 2 cut(s) 364, 660
HphI GGTGA 1 cut(s) 778
Hpy166II GTNNAC 2 cut(s) 336, 548
Hpy188I TCNGA 4 cut(s) 47, 184, 510, 889
Hpy188III TCNNGA 3 cut(s) 261, 377, 533
Hpy8I GTNNAC 2 cut(s) 336, 548
HpyAV CCTTC 3 cut(s) 73, 449, 584
HpyCH4III ACNGT 3 cut(s) 545, 935, 973
HpyCH4IV ACGT 2 cut(s) 138, 249
HpyCH4V TGCA 9 cut(s) 158, 189, 347, 523, 649, 761, 782, 868, 880
HpyF10VI GCNNNNNNNGC 1 cut(s) 758
HpySE526I ACGT 2 cut(s) 138, 249
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 521, 577, 649, 710
HspAI GCGC 1 cut(s) 219
KasI GGCGCC 1 cut(s) 218
KspAI GTTAAC 1 cut(s) 336
Kzo9I GATC 3 cut(s) 280, 353, 418
LmnI GCTCC 2 cut(s) 28, 88
Lsp1109I GCAGC 2 cut(s) 32, 927
LweI GCATC 5 cut(s) 145, 234, 532, 748, 802
MaeI CTAG 2 cut(s) 533, 602
MaeII ACGT 2 cut(s) 138, 249
MaeIII GTNAC 1 cut(s) 796
MalI GATC 3 cut(s) 282, 355, 420
MboI GATC 3 cut(s) 280, 353, 418
MboII GAAGA 4 cut(s) 93, 99, 133, 220
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 2 cut(s) 93, 237
MlsI TGGCCA 2 cut(s) 173, 295
MluCI AATT 2 cut(s) 269, 693
MluNI TGGCCA 2 cut(s) 173, 295
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 1 cut(s) 92
Mox20I TGGCCA 2 cut(s) 173, 295
MscI TGGCCA 2 cut(s) 173, 295
MseI TTAA 4 cut(s) 288, 335, 671, 696
MslI CAYNNNNRTG 2 cut(s) 230, 518
Msp20I TGGCCA 2 cut(s) 173, 295
MspI CCGG 2 cut(s) 364, 660
MspR9I CCNGG 4 cut(s) 84, 365, 428, 640
MvaI CCWGG 3 cut(s) 84, 428, 640
MwoI GCNNNNNNNGC 1 cut(s) 758
NarI GGCGCC 1 cut(s) 219
NciI CCSGG 1 cut(s) 365
NcoI CCATGG 1 cut(s) 573
NdeII GATC 3 cut(s) 280, 353, 418
NlaIII CATG 4 cut(s) 521, 577, 649, 710
NlaIV GGNNCC 3 cut(s) 90, 220, 369
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 796
NspI RCATGY 1 cut(s) 649
OliI CACNNNNGTG 1 cut(s) 518
PasI CCCWGGG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PfeI GAWTC 2 cut(s) 78, 132
PkrI GCNGC 2 cut(s) 22, 942
PleI GAGTC 1 cut(s) 92
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 92
Psp6I CCWGG 3 cut(s) 82, 426, 638
PspFI CCCAGC 1 cut(s) 815
PspGI CCWGG 3 cut(s) 82, 426, 638
PspN4I GGNNCC 3 cut(s) 90, 220, 369
PspPI GGNCC 3 cut(s) 60, 506, 818
PsuI RGATCY 1 cut(s) 353
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
RseI CAYNNNNRTG 2 cut(s) 230, 518
SaqAI TTAA 4 cut(s) 288, 335, 671, 696
SatI GCNGC 2 cut(s) 21, 941
Sau3AI GATC 3 cut(s) 280, 353, 418
Sau96I GGNCC 3 cut(s) 60, 506, 818
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 4 cut(s) 84, 365, 428, 640
SduI GDGCHC 2 cut(s) 93, 237
SfaNI GCATC 5 cut(s) 145, 234, 532, 748, 802
SfoI GGCGCC 1 cut(s) 220
SinI GGWCC 1 cut(s) 506
SmiMI CAYNNNNRTG 2 cut(s) 230, 518
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 2 cut(s) 269, 693
SsiI CCGC 1 cut(s) 204
SspDI GGCGCC 1 cut(s) 218
SspMI CTAG 2 cut(s) 533, 602
StyD4I CCNGG 4 cut(s) 82, 363, 426, 638
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 3 cut(s) 545, 935, 973
TaiI ACGT 2 cut(s) 141, 252
TaqI TCGA 4 cut(s) 96, 279, 502, 897
TasI AATT 2 cut(s) 269, 693
TfiI GAWTC 2 cut(s) 78, 132
Tru1I TTAA 4 cut(s) 288, 335, 671, 696
Tru9I TTAA 4 cut(s) 288, 335, 671, 696
TscAI CASTG 2 cut(s) 452, 625
TseFI GTSAC 1 cut(s) 796
TseI GCWGC 2 cut(s) 20, 940
Tsp45I GTSAC 1 cut(s) 796
TspDTI ATGAA 3 cut(s) 17, 93, 692
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 2 cut(s) 452, 625
VpaK11BI GGWCC 1 cut(s) 506
XapI RAATTY 1 cut(s) 269
XbaI TCTAGA 1 cut(s) 532
XceI RCATGY 1 cut(s) 649
XmiI GTMKAC 1 cut(s) 547
XspI CTAG 2 cut(s) 533, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.