pycom06g05490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014830
Physical Location & Seq
Reverse (-)
7950302 .. 7950815
514 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g05490.1

Sequence Viewer

Length: 423 bp
ATGAACAAAAGTGAAGCTTGCAGCCAATCAAAAAGAAAAAAGAAAAGAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGAAAAAAGCTTCCCCTGCACCCATACCCGCACCACCGCGAGTCCATCTTCCAAGGCCGATTCCGGCCAACTCCGGTGGAGTTTTTGGATGCCACCACTACCATCTTGAAGCTCTCATTCCCCTCTACAAAACCCACCCAAGAACCACCTTGATTAACCATGGTATGGGGCGGTTTGAGGCCACGAAAGTTGACGAAAATCAATGGTGCTCCGGCCACCCTTTTCGATTTTCCGGCAAATCGGCAAAGCAATGGCCAATGCCACCACTTGGGCTTTGTAGCCCATCATCCCATGAGCAAAACCCAACCAACCTTGACCCTGTTGGACCACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.67

Weight (kDa)

10.33

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 253, 356
AccII CGCG 1 cut(s) 127
AciI CCGC 3 cut(s) 117, 125, 259
AcoI YGGCCR 3 cut(s) 153, 301, 341
AfiI CCNNNNNNNGG 2 cut(s) 253, 356
AgsI TTSAA 1 cut(s) 197
AluBI AGCT 3 cut(s) 17, 98, 200
AluI AGCT 3 cut(s) 17, 98, 200
Alw21I GWGCWC 1 cut(s) 299
AoxI GGCC 5 cut(s) 143, 153, 267, 301, 341
ApeKI GCWGC 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 413
AvaII GGWCC 1 cut(s) 413
BalI TGGCCA 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 1 cut(s) 33
BccI CCATC 3 cut(s) 141, 198, 379
BfmI CTRYAG 1 cut(s) 419
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
Bme18I GGWCC 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 413
BmsI GCATC 1 cut(s) 167
BsaJI CCNNGG 2 cut(s) 140, 247
BsaWI WCCGGW 1 cut(s) 161
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 356
Bse3DI GCAATG 1 cut(s) 344
BseDI CCNNGG 2 cut(s) 140, 247
BseGI GGATG 2 cut(s) 182, 374
BseLI CCNNNNNNNGG 2 cut(s) 253, 356
BseMI GCAATG 1 cut(s) 344
BseXI GCAGC 1 cut(s) 33
BsgI GTGCAG 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 127
BshFI GGCC 5 cut(s) 145, 155, 269, 303, 343
BsiHKAI GWGCWC 1 cut(s) 299
BsiSI CCGG 4 cut(s) 152, 162, 300, 321
BslI CCNNNNNNNGG 2 cut(s) 253, 356
BsnI GGCC 5 cut(s) 145, 155, 269, 303, 343
Bsp1286I GDGCHC 1 cut(s) 299
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 3 cut(s) 117, 125, 259
BspANI GGCC 5 cut(s) 145, 155, 269, 303, 343
BspFNI CGCG 1 cut(s) 127
BsrDI GCAATG 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 140, 247
BssT1I CCWWGG 2 cut(s) 140, 247
Bst4CI ACNGT 1 cut(s) 420
BstC8I GCNNGC 1 cut(s) 19
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 2 cut(s) 182, 374
BstFNI CGCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 419
BstUI CGCG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 33
BsuRI GGCC 5 cut(s) 145, 155, 269, 303, 343
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 2 cut(s) 182, 374
BtsIMutI CAGTG 1 cut(s) 416
Cac8I GCNNGC 1 cut(s) 19
Cfr13I GGNCC 1 cut(s) 413
CspCI CAANNNNNGTGG 4 cut(s) 145, 180, 259, 294
CviAII CATG 2 cut(s) 248, 380
EaeI YGGCCR 3 cut(s) 153, 301, 341
Eco130I CCWWGG 2 cut(s) 140, 247
Eco47I GGWCC 1 cut(s) 413
EcoT14I CCWWGG 2 cut(s) 140, 247
ErhI CCWWGG 2 cut(s) 140, 247
FaeI CATG 2 cut(s) 251, 383
FaiI YATR 4 cut(s) 113, 249, 254, 381
FalI AAGNNNNNCTT 1 cut(s) 33
FatI CATG 2 cut(s) 247, 379
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 1 cut(s) 22
FokI GGATG 2 cut(s) 189, 361
Fsp4HI GCNGC 1 cut(s) 22
GluI GCNGC 1 cut(s) 22
HaeIII GGCC 5 cut(s) 145, 155, 269, 303, 343
HapII CCGG 4 cut(s) 152, 162, 300, 321
Hin1II CATG 2 cut(s) 251, 383
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 2 cut(s) 15, 96
HinfI GANTC 2 cut(s) 129, 148
HpaII CCGG 4 cut(s) 152, 162, 300, 321
Hpy166II GTNNAC 1 cut(s) 280
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 1 cut(s) 280
HpyCH4III ACNGT 1 cut(s) 420
HpyCH4V TGCA 2 cut(s) 21, 107
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
Hsp92II CATG 2 cut(s) 251, 383
LmnI GCTCC 1 cut(s) 302
LpnPI CCDG 5 cut(s) 117, 165, 175, 313, 334
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 1 cut(s) 167
MboII GAAGA 1 cut(s) 128
MhlI GDGCHC 1 cut(s) 299
MlsI TGGCCA 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 343
MlyI GAGTC 1 cut(s) 138
MmeI TCCRAC 1 cut(s) 391
MnlI CCTC 2 cut(s) 221, 259
Mox20I TGGCCA 1 cut(s) 343
MscI TGGCCA 1 cut(s) 343
MseI TTAA 1 cut(s) 243
Msp20I TGGCCA 1 cut(s) 343
MspI CCGG 4 cut(s) 152, 162, 300, 321
MvnI CGCG 1 cut(s) 127
MwoI GCNNNNNNNGC 1 cut(s) 104
NcoI CCATGG 1 cut(s) 247
NlaIII CATG 2 cut(s) 251, 383
PfeI GAWTC 1 cut(s) 148
PflMI CCANNNNNTGG 2 cut(s) 253, 356
PkrI GCNGC 1 cut(s) 23
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 1 cut(s) 22
Sau96I GGNCC 1 cut(s) 413
SchI GAGTC 1 cut(s) 138
SduI GDGCHC 1 cut(s) 299
SetI ASST 5 cut(s) 19, 100, 202, 239, 402
SfaNI GCATC 1 cut(s) 167
SfcI CTRYAG 1 cut(s) 419
SinI GGWCC 1 cut(s) 413
SsiI CCGC 3 cut(s) 117, 125, 259
StyI CCWWGG 2 cut(s) 140, 247
TaaI ACNGT 1 cut(s) 420
TaqI TCGA 1 cut(s) 313
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 1 cut(s) 423
TseI GCWGC 1 cut(s) 21
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 423
Van91I CCANNNNNTGG 2 cut(s) 253, 356
VpaK11BI GGWCC 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.