pycom12g15410

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014830
Physical Location & Seq
Reverse (-)
17757072 .. 17757713
642 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g15410.3

Sequence Viewer

Length: 549 bp
ATGTGGGACTTATTCTTTAACCGTTTGCGCGTCCGGATTATAACGGTATATAAACCCCCACTTTGTGGGGGAAGCCCAAAAATCAGAAACAGAGGGAGGAAAGAAAGAAAGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGGAAGAGAGCTTCCCGTGCACCCATACCCGCGCCACCACGAGACCATCTTCCAAGGCCGATTCCGGCCAACTCCGGTGGATTTTTTCGATGCCACCACCACCATCTTGAAGCTCTCATTCCCCTCTACAAAACCCACCCAAGAACCACCTTGATTAACCATGGTATGGAGCGGTTTGAGGCCACGAAAGTTGACGAAAATCAATGGTGCTCCGGCCACCCTTTTCGATTTTCCGGCAAATCGGCAAAGCGATGGCCGATGCCACCACTTGGGCTTCGTAGCCCATCATCCCAGGAGCAAAACCCAACCAACCTTGACCCCGTTGGACCACCGTGGACGACGAATCGACGTCGGGAAGTTTTAGGCACCCGCCGGCTTCGTGGGCAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

21.35

Weight (kDa)

11.52

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
AatII GACGTC 1 cut(s) 508
AccB1I GGYRCC 1 cut(s) 521
AccB7I CCANNNNNTGG 3 cut(s) 65, 322, 425
AccBSI CCGCTC 1 cut(s) 328
AccII CGCG 2 cut(s) 30, 188
AccIII TCCGGA 1 cut(s) 33
AciI CCGC 3 cut(s) 186, 328, 526
AcoI YGGCCR 3 cut(s) 222, 370, 410
AcyI GRCGYC 1 cut(s) 505
AdeI CACNNNGTG 1 cut(s) 65
AfiI CCNNNNNNNGG 3 cut(s) 65, 322, 425
AgsI TTSAA 1 cut(s) 266
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 2 cut(s) 167, 269
AluI AGCT 2 cut(s) 167, 269
Alw21I GWGCWC 2 cut(s) 178, 368
Alw26I GTCTC 1 cut(s) 192
Alw44I GTGCAC 1 cut(s) 174
Aor13HI TCCGGA 1 cut(s) 33
AoxI GGCC 5 cut(s) 212, 222, 336, 370, 410
ApaLI GTGCAC 1 cut(s) 174
AspLEI GCGC 2 cut(s) 30, 190
AspS9I GGNCC 1 cut(s) 482
AvaII GGWCC 1 cut(s) 482
BaeGI GKGCMC 1 cut(s) 178
BanI GGYRCC 1 cut(s) 521
BauI CACGAG 1 cut(s) 195
Bbv12I GWGCWC 2 cut(s) 178, 368
BccI CCATC 4 cut(s) 210, 267, 402, 448
BciT130I CCWGG 1 cut(s) 449
BcoDI GTCTC 1 cut(s) 192
Bme1390I CCNGG 1 cut(s) 449
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 1 cut(s) 523
BmrFI CCNGG 1 cut(s) 449
BmsI GCATC 2 cut(s) 236, 405
BsaHI GRCGYC 1 cut(s) 505
BsaI GGTCTC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 209, 316, 447, 488
BsaWI WCCGGW 2 cut(s) 33, 230
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 322, 425
Bse118I RCCGGY 1 cut(s) 528
BseAI TCCGGA 1 cut(s) 33
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 4 cut(s) 209, 316, 447, 488
BseGI GGATG 1 cut(s) 443
BseLI CCNNNNNNNGG 3 cut(s) 65, 322, 425
BseSI GKGCMC 1 cut(s) 178
Bsh1236I CGCG 2 cut(s) 30, 188
BshFI GGCC 5 cut(s) 214, 224, 338, 372, 412
BshNI GGYRCC 1 cut(s) 521
BsiHKAI GWGCWC 2 cut(s) 178, 368
BsiSI CCGG 6 cut(s) 34, 221, 231, 369, 390, 529
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 3 cut(s) 65, 322, 425
BsmAI GTCTC 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 5 cut(s) 214, 224, 338, 372, 412
Bso31I GGTCTC 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 178, 368
Bsp13I TCCGGA 1 cut(s) 33
Bsp19I CCATGG 1 cut(s) 316
BspACI CCGC 3 cut(s) 186, 328, 526
BspANI GGCC 5 cut(s) 214, 224, 338, 372, 412
BspEI TCCGGA 1 cut(s) 33
BspFNI CGCG 2 cut(s) 30, 188
BspLI GGNNCC 1 cut(s) 523
BspT107I GGYRCC 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 192
BsrBI CCGCTC 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 528
BssAI RCCGGY 1 cut(s) 528
BssECI CCNNGG 4 cut(s) 209, 316, 447, 488
BssNI GRCGYC 1 cut(s) 505
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 2 cut(s) 209, 316
Bst2BI CACGAG 1 cut(s) 195
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 3 cut(s) 23, 46, 489
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 505
BstC8I GCNNGC 1 cut(s) 530
BstDSI CCRYGG 2 cut(s) 316, 488
BstF5I GGATG 1 cut(s) 443
BstFNI CGCG 2 cut(s) 30, 188
BstHHI GCGC 2 cut(s) 30, 190
BstMAI GTCTC 1 cut(s) 192
BstMWI GCNNNNNNNGC 2 cut(s) 173, 538
BstNI CCWGG 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 447
BstSLI GKGCMC 1 cut(s) 178
BstUI CGCG 2 cut(s) 30, 188
BsuRI GGCC 5 cut(s) 214, 224, 338, 372, 412
BtgI CCRYGG 2 cut(s) 316, 488
BtgZI GCGATG 1 cut(s) 421
BtsCI GGATG 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 530
CfoI GCGC 2 cut(s) 30, 190
Cfr10I RCCGGY 1 cut(s) 528
Cfr13I GGNCC 1 cut(s) 482
CseI GACGC 1 cut(s) 19
CspCI CAANNNNNGTGG 4 cut(s) 214, 249, 328, 363
CviAII CATG 1 cut(s) 317
DraIII CACNNNGTG 1 cut(s) 65
EaeI YGGCCR 3 cut(s) 222, 370, 410
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Eco130I CCWWGG 2 cut(s) 209, 316
Eco31I GGTCTC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 2 cut(s) 209, 316
ErhI CCWWGG 2 cut(s) 209, 316
FaeI CATG 1 cut(s) 320
FaiI YATR 6 cut(s) 41, 49, 51, 182, 318, 323
FaqI GGGAC 1 cut(s) 20
FatI CATG 1 cut(s) 316
FauI CCCGC 2 cut(s) 193, 533
FokI GGATG 1 cut(s) 430
GlaI GCGC 2 cut(s) 29, 189
HaeIII GGCC 5 cut(s) 214, 224, 338, 372, 412
HapII CCGG 6 cut(s) 34, 221, 231, 369, 390, 529
HgaI GACGC 1 cut(s) 19
HhaI GCGC 2 cut(s) 30, 190
Hin1I GRCGYC 1 cut(s) 505
Hin1II CATG 1 cut(s) 320
Hin6I GCGC 2 cut(s) 28, 188
HinP1I GCGC 2 cut(s) 28, 188
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HinfI GANTC 2 cut(s) 217, 499
HpaII CCGG 6 cut(s) 34, 221, 231, 369, 390, 529
Hpy166II GTNNAC 3 cut(s) 176, 349, 492
Hpy188I TCNGA 1 cut(s) 86
Hpy188III TCNNGA 3 cut(s) 34, 263, 509
Hpy8I GTNNAC 3 cut(s) 176, 349, 492
Hpy99I CGWCG 3 cut(s) 499, 507, 510
HpyCH4III ACNGT 3 cut(s) 23, 46, 489
HpyCH4IV ACGT 1 cut(s) 505
HpyCH4V TGCA 1 cut(s) 176
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 538
HpySE526I ACGT 1 cut(s) 505
Hsp92I GRCGYC 1 cut(s) 505
Hsp92II CATG 1 cut(s) 320
HspAI GCGC 2 cut(s) 28, 188
Kpn2I TCCGGA 1 cut(s) 33
KroI GCCGGC 1 cut(s) 528
KroNI GCCGGC 1 cut(s) 530
LmnI GCTCC 3 cut(s) 325, 371, 451
LpnPI CCDG 8 cut(s) 47, 234, 244, 382, 403, 434, 461, 542
LweI GCATC 2 cut(s) 236, 405
MaeII ACGT 1 cut(s) 505
MbiI CCGCTC 1 cut(s) 328
MboII GAAGA 2 cut(s) 172, 197
MhlI GDGCHC 2 cut(s) 178, 368
MluCI AATT 1 cut(s) 544
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 4 cut(s) 86, 90, 290, 328
MroI TCCGGA 1 cut(s) 33
MroNI GCCGGC 1 cut(s) 528
MseI TTAA 2 cut(s) 18, 312
MspI CCGG 6 cut(s) 34, 221, 231, 369, 390, 529
MspR9I CCNGG 1 cut(s) 449
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 2 cut(s) 30, 188
MwoI GCNNNNNNNGC 2 cut(s) 173, 538
NaeI GCCGGC 1 cut(s) 530
NcoI CCATGG 1 cut(s) 316
NgoMIV GCCGGC 1 cut(s) 528
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 1 cut(s) 523
PdiI GCCGGC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 217, 499
PflMI CCANNNNNTGG 3 cut(s) 65, 322, 425
PsiI TTATAA 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 447
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 1 cut(s) 523
PspPI GGNCC 1 cut(s) 482
SaqAI TTAA 2 cut(s) 18, 312
Sau96I GGNCC 1 cut(s) 482
ScrFI CCNGG 1 cut(s) 449
SduI GDGCHC 2 cut(s) 178, 368
SetI ASST 5 cut(s) 169, 271, 308, 471, 508
SfaNI GCATC 2 cut(s) 236, 405
SinI GGWCC 1 cut(s) 482
Sse9I AATT 1 cut(s) 544
SsiI CCGC 3 cut(s) 186, 328, 526
StyD4I CCNGG 1 cut(s) 447
StyI CCWWGG 2 cut(s) 209, 316
TaaI ACNGT 3 cut(s) 23, 46, 489
TaiI ACGT 1 cut(s) 508
TaqI TCGA 3 cut(s) 244, 382, 502
TasI AATT 1 cut(s) 544
TfiI GAWTC 2 cut(s) 217, 499
Tru1I TTAA 2 cut(s) 18, 312
Tru9I TTAA 2 cut(s) 18, 312
Van91I CCANNNNNTGG 3 cut(s) 65, 322, 425
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 1 cut(s) 482
ZraI GACGTC 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.