pycom08g11140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014830
Physical Location & Seq
Forward (+)
9441486 .. 9442055
570 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11140.1

Sequence Viewer

Length: 483 bp
ATGTGGGACTTATTCTTTAAGCATATCCTCGTAGTACTCTTTGGTACGAACTTACGGACGTTCGAAATTGGTTATTCAAGCCCAAAATCAGAAACGGAGGGAGGAAAGAAAGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGAAGAGAGCTTCCCGTGCACCCATACCTGCGCCACCACGAGACCATCTTCCAAGGCCGATTCTGGCCAACTCCGGTGGAGTTTTTCGATGCCACCACCACCATCTTGAAGCTCTCATTCCCCTCTACAAAACCCACCCAAGAACCACCTTGATTGACCATGGTATGAGGCGGTTTGAGGCCACGAAAGTTGACGAAAATCAATGGTGCTCCGGCCACCCTTTTCGATTTTTCGGCAAATCGGCAAAGCAATGGCCGATGCCACCACTTGGGCTTTGTAGCCCATCATCCCAGGAGCAAAACCCAACCAACCTTGACCCCGTTGGACCACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.27

Weight (kDa)

10.21

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 416
AciI CCGC 1 cut(s) 319
AcoI YGGCCR 3 cut(s) 213, 361, 401
AfaI GTAC 2 cut(s) 36, 46
AfiI CCNNNNNNNGG 1 cut(s) 416
AgsI TTSAA 2 cut(s) 78, 257
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 2 cut(s) 158, 260
AluI AGCT 2 cut(s) 158, 260
Alw21I GWGCWC 2 cut(s) 169, 359
Alw26I GTCTC 1 cut(s) 183
Alw44I GTGCAC 1 cut(s) 165
AoxI GGCC 5 cut(s) 203, 213, 327, 361, 401
ApaLI GTGCAC 1 cut(s) 165
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 1 cut(s) 473
AsuII TTCGAA 1 cut(s) 63
AvaII GGWCC 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 169
BalI TGGCCA 1 cut(s) 215
BauI CACGAG 1 cut(s) 186
Bbv12I GWGCWC 2 cut(s) 169, 359
BccI CCATC 3 cut(s) 201, 258, 439
BciT130I CCWGG 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 184
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 440
Bme18I GGWCC 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 473
BmrFI CCNGG 1 cut(s) 440
BmsI GCATC 2 cut(s) 227, 396
Bpu14I TTCGAA 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 3 cut(s) 200, 307, 438
BsaWI WCCGGW 1 cut(s) 221
Bsc4I CCNNNNNNNGG 1 cut(s) 416
Bse3DI GCAATG 1 cut(s) 404
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 3 cut(s) 200, 307, 438
BseGI GGATG 1 cut(s) 434
BseLI CCNNNNNNNGG 1 cut(s) 416
BseMI GCAATG 1 cut(s) 404
BseSI GKGCMC 1 cut(s) 169
BshFI GGCC 5 cut(s) 205, 215, 329, 363, 403
BsiHKAI GWGCWC 2 cut(s) 169, 359
BsiSI CCGG 2 cut(s) 222, 360
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 416
BsmAI GTCTC 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 5 cut(s) 205, 215, 329, 363, 403
Bso31I GGTCTC 1 cut(s) 183
Bsp119I TTCGAA 1 cut(s) 63
Bsp1286I GDGCHC 2 cut(s) 169, 359
Bsp19I CCATGG 1 cut(s) 307
BspACI CCGC 1 cut(s) 319
BspANI GGCC 5 cut(s) 205, 215, 329, 363, 403
BspMI ACCTGC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 63
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 404
BssECI CCNNGG 3 cut(s) 200, 307, 438
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 200, 307
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 1 cut(s) 480
Bst6I CTCTTC 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 63
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 1 cut(s) 434
BstHHI GCGC 1 cut(s) 181
BstMAI GTCTC 1 cut(s) 183
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 440
BstSCI CCNGG 1 cut(s) 438
BstSLI GKGCMC 1 cut(s) 169
BsuRI GGCC 5 cut(s) 205, 215, 329, 363, 403
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 1 cut(s) 434
BveI ACCTGC 1 cut(s) 184
CfoI GCGC 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 473
Csp6I GTAC 2 cut(s) 35, 45
CspCI CAANNNNNGTGG 6 cut(s) 205, 240, 283, 318, 319, 354
CviAII CATG 1 cut(s) 308
CviQI GTAC 2 cut(s) 35, 45
EaeI YGGCCR 3 cut(s) 213, 361, 401
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
Eco130I CCWWGG 2 cut(s) 200, 307
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 2 cut(s) 200, 307
ErhI CCWWGG 2 cut(s) 200, 307
FaeI CATG 1 cut(s) 311
FaiI YATR 4 cut(s) 24, 173, 309, 314
FaqI GGGAC 1 cut(s) 20
FatI CATG 1 cut(s) 307
FokI GGATG 1 cut(s) 421
GlaI GCGC 1 cut(s) 180
HaeIII GGCC 5 cut(s) 205, 215, 329, 363, 403
HapII CCGG 2 cut(s) 222, 360
HhaI GCGC 1 cut(s) 181
Hin1II CATG 1 cut(s) 311
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 1 cut(s) 208
HpaII CCGG 2 cut(s) 222, 360
Hpy166II GTNNAC 2 cut(s) 167, 340
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 2 cut(s) 167, 340
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 1 cut(s) 167
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpySE526I ACGT 1 cut(s) 59
Hsp92II CATG 1 cut(s) 311
HspAI GCGC 1 cut(s) 179
LmnI GCTCC 2 cut(s) 362, 442
LpnPI CCDG 6 cut(s) 189, 197, 235, 373, 425, 452
LweI GCATC 2 cut(s) 227, 396
MaeII ACGT 1 cut(s) 59
MboII GAAGA 2 cut(s) 163, 188
MhlI GDGCHC 2 cut(s) 169, 359
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 1 cut(s) 66
MluNI TGGCCA 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 451
MnlI CCTC 6 cut(s) 38, 91, 95, 281, 309, 319
Mox20I TGGCCA 1 cut(s) 215
MscI TGGCCA 1 cut(s) 215
MseI TTAA 1 cut(s) 18
Msp20I TGGCCA 1 cut(s) 215
MspI CCGG 2 cut(s) 222, 360
MspR9I CCNGG 1 cut(s) 440
MvaI CCWGG 1 cut(s) 440
MwoI GCNNNNNNNGC 1 cut(s) 164
NcoI CCATGG 1 cut(s) 307
NlaIII CATG 1 cut(s) 311
NspV TTCGAA 1 cut(s) 63
PfeI GAWTC 1 cut(s) 208
PflMI CCANNNNNTGG 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspPI GGNCC 1 cut(s) 473
RsaI GTAC 2 cut(s) 36, 46
RsaNI GTAC 2 cut(s) 35, 45
SaqAI TTAA 1 cut(s) 18
Sau96I GGNCC 1 cut(s) 473
ScaI AGTACT 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 440
SduI GDGCHC 2 cut(s) 169, 359
SetI ASST 6 cut(s) 62, 160, 178, 262, 299, 462
SfaNI GCATC 2 cut(s) 227, 396
SfuI TTCGAA 1 cut(s) 63
SinI GGWCC 1 cut(s) 473
Sse9I AATT 1 cut(s) 66
SsiI CCGC 1 cut(s) 319
StyD4I CCNGG 1 cut(s) 438
StyI CCWWGG 2 cut(s) 200, 307
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 1 cut(s) 62
TaqI TCGA 3 cut(s) 63, 235, 373
TasI AATT 1 cut(s) 66
TatI WGTACW 1 cut(s) 34
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TspGWI ACGGA 2 cut(s) 70, 110
Van91I CCANNNNNTGG 1 cut(s) 416
VneI GTGCAC 1 cut(s) 165
VpaK11BI GGWCC 1 cut(s) 473
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.