pycom1481g00070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00001481
OG0014830
Physical Location & Seq
Forward (+)
184984 .. 185483
500 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom1481g00070.1

Sequence Viewer

Length: 414 bp
ATGGACAAAATAAAAGTGAAGCTTGCAGCCAATCAAAAAGAAAGAAGAAAAGAAAAGAAAAAAAGAAAAAAAAAAAAAGGGAAGAAAGCTTCCCGTGCACCCATACCCGCACCACCGCGAGTCCATCTTCCAAGGCCGATTCCGGCCAACTCCGGTGAAGTTTTTCGATGCCACCACCACCATCTTGAAGCTCTCATTCCCCTCTACAAAACCCACCCAAGAACCACCTTGATTAACCATGGTATGGGGCGGTTTGAGGCCACGAAAGTTGACGAAAATCAATGGTGCTCCGGCCACCCTTTTCGATTTTCCGGCAAATCGGCAAAGCAATGGCCGATGCCACCACTTGGGCTTTGTAGCCCATCATCCCAGGAGCAAAACCCAACCAACCTTGACCCTGTTGGACCACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.51

Weight (kDa)

10.43

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 244, 347
AccII CGCG 1 cut(s) 118
AciI CCGC 3 cut(s) 108, 116, 250
AcoI YGGCCR 3 cut(s) 144, 292, 332
AfiI CCNNNNNNNGG 2 cut(s) 244, 347
AgsI TTSAA 1 cut(s) 188
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 3 cut(s) 22, 89, 191
AluI AGCT 3 cut(s) 22, 89, 191
Alw21I GWGCWC 2 cut(s) 100, 290
Alw44I GTGCAC 1 cut(s) 96
AoxI GGCC 5 cut(s) 134, 144, 258, 292, 332
ApaLI GTGCAC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 26
Asp700I GAANNNNTTC 1 cut(s) 162
AspS9I GGNCC 1 cut(s) 404
AsuHPI GGTGA 1 cut(s) 167
AvaII GGWCC 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 100
Bbv12I GWGCWC 2 cut(s) 100, 290
BbvI GCAGC 1 cut(s) 38
BccI CCATC 3 cut(s) 132, 189, 370
BciT130I CCWGG 1 cut(s) 371
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 371
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 404
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 2 cut(s) 158, 327
BsaJI CCNNGG 3 cut(s) 131, 238, 369
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 347
Bse3DI GCAATG 1 cut(s) 335
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 3 cut(s) 131, 238, 369
BseGI GGATG 1 cut(s) 365
BseLI CCNNNNNNNGG 2 cut(s) 244, 347
BseMI GCAATG 1 cut(s) 335
BseSI GKGCMC 1 cut(s) 100
BseXI GCAGC 1 cut(s) 38
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 5 cut(s) 136, 146, 260, 294, 334
BsiHKAI GWGCWC 2 cut(s) 100, 290
BsiSI CCGG 4 cut(s) 143, 153, 291, 312
BslI CCNNNNNNNGG 2 cut(s) 244, 347
BsnI GGCC 5 cut(s) 136, 146, 260, 294, 334
Bsp1286I GDGCHC 2 cut(s) 100, 290
Bsp19I CCATGG 1 cut(s) 238
BspACI CCGC 3 cut(s) 108, 116, 250
BspANI GGCC 5 cut(s) 136, 146, 260, 294, 334
BspFNI CGCG 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 335
BssECI CCNNGG 3 cut(s) 131, 238, 369
BssT1I CCWWGG 2 cut(s) 131, 238
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 411
BstC8I GCNNGC 1 cut(s) 24
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 365
BstFNI CGCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstNI CCWGG 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 100
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 38
BsuRI GGCC 5 cut(s) 136, 146, 260, 294, 334
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 1 cut(s) 365
Cac8I GCNNGC 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 404
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviAII CATG 1 cut(s) 239
EaeI YGGCCR 3 cut(s) 144, 292, 332
Eco130I CCWWGG 2 cut(s) 131, 238
Eco47I GGWCC 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 369
EcoT14I CCWWGG 2 cut(s) 131, 238
ErhI CCWWGG 2 cut(s) 131, 238
FaeI CATG 1 cut(s) 242
FaiI YATR 3 cut(s) 104, 240, 245
FalI AAGNNNNNCTT 1 cut(s) 38
FatI CATG 1 cut(s) 238
FauI CCCGC 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 1 cut(s) 352
Fsp4HI GCNGC 1 cut(s) 27
GluI GCNGC 1 cut(s) 27
HaeIII GGCC 5 cut(s) 136, 146, 260, 294, 334
HapII CCGG 4 cut(s) 143, 153, 291, 312
Hin1II CATG 1 cut(s) 242
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 2 cut(s) 20, 87
HinfI GANTC 2 cut(s) 120, 139
HpaII CCGG 4 cut(s) 143, 153, 291, 312
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 2 cut(s) 98, 271
Hpy188III TCNNGA 1 cut(s) 185
Hpy8I GTNNAC 2 cut(s) 98, 271
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4V TGCA 2 cut(s) 26, 98
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
Hsp92II CATG 1 cut(s) 242
LmnI GCTCC 2 cut(s) 293, 373
LpnPI CCDG 6 cut(s) 156, 166, 304, 325, 356, 383
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 2 cut(s) 158, 327
MboII GAAGA 3 cut(s) 57, 94, 119
MhlI GDGCHC 2 cut(s) 100, 290
MlyI GAGTC 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 382
MnlI CCTC 2 cut(s) 212, 250
MroXI GAANNNNTTC 1 cut(s) 162
MseI TTAA 1 cut(s) 234
MspI CCGG 4 cut(s) 143, 153, 291, 312
MspR9I CCNGG 1 cut(s) 371
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 1 cut(s) 95
NcoI CCATGG 1 cut(s) 238
NlaIII CATG 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 1 cut(s) 139
PflMI CCANNNNNTGG 2 cut(s) 244, 347
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspPI GGNCC 1 cut(s) 404
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 1 cut(s) 27
Sau96I GGNCC 1 cut(s) 404
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 2 cut(s) 100, 290
SetI ASST 5 cut(s) 24, 91, 193, 230, 393
SfaNI GCATC 2 cut(s) 158, 327
SinI GGWCC 1 cut(s) 404
SsiI CCGC 3 cut(s) 108, 116, 250
StyD4I CCNGG 1 cut(s) 369
StyI CCWWGG 2 cut(s) 131, 238
TaaI ACNGT 1 cut(s) 411
TaqI TCGA 2 cut(s) 166, 304
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TseI GCWGC 1 cut(s) 26
Van91I CCANNNNNTGG 2 cut(s) 244, 347
VneI GTGCAC 1 cut(s) 96
VpaK11BI GGWCC 1 cut(s) 404
XmnI GAANNNNTTC 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.