pycom08g20710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014830
Physical Location & Seq
Reverse (-)
20395407 .. 20395964
558 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20710.1

Sequence Viewer

Length: 468 bp
ATGTGGGACTTATTCTTTAAGCATATCCTCGTAGTACTCTTTGGTACGAACTTACGAACGTTCGAAATTGGTTATTCAAGGTTTAGTGTAAACGGAGGGAGGAAAGGAAAGAAGAAAAAAAAAAAAAAAACAGGGAAGAGAGCTTCCCGTGCCCCCATACCCGCGCCCCCACGAAACCACCTTCCAAGGCCGATTCCGGCCAACTCCGGTGGAGTTTTTCGATGCCACCACCACCATCTTGAAGCTCTCATTCCCCTCTACAAAACTCACCCAAGAACCACCTTGATTGACCATGGTATGAGACGGTTTGAGGCCACGAAAGTTGACGAAAATCAATGGTGCTCCGGCCACCCTTTTCGATTTTCCGGCCAATCGGCAAAGCAATGGCCGATGCCACCACTTGGGCTTTGTAGCCCACAATCCCAGGAGCAAAACCCAACCAACCTTGACCCCGTTGGACCACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.59

Weight (kDa)

10.55

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 401
AccII CGCG 1 cut(s) 164
AciI CCGC 1 cut(s) 162
AclI AACGTT 1 cut(s) 59
AcoI YGGCCR 4 cut(s) 198, 346, 367, 386
AfaI GTAC 2 cut(s) 36, 46
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 2 cut(s) 78, 242
AjnI CCWGG 1 cut(s) 423
AluBI AGCT 2 cut(s) 143, 245
AluI AGCT 2 cut(s) 143, 245
Alw21I GWGCWC 1 cut(s) 344
Alw26I GTCTC 1 cut(s) 295
AoxI GGCC 6 cut(s) 188, 198, 312, 346, 367, 386
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 1 cut(s) 458
AsuHPI GGTGA 1 cut(s) 260
AsuII TTCGAA 1 cut(s) 63
AvaII GGWCC 1 cut(s) 458
BaeGI GKGCMC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 344
BccI CCATC 1 cut(s) 243
BciT130I CCWGG 1 cut(s) 425
BcoDI GTCTC 1 cut(s) 295
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 425
Bme18I GGWCC 1 cut(s) 458
BmgT120I GGNCC 1 cut(s) 458
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 2 cut(s) 212, 381
Bpu14I TTCGAA 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 185, 292, 423
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse3DI GCAATG 1 cut(s) 389
BseBI CCWGG 1 cut(s) 425
BseDI CCNNGG 3 cut(s) 185, 292, 423
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 389
BseSI GKGCMC 1 cut(s) 154
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 6 cut(s) 190, 200, 314, 348, 369, 388
BsiHKAI GWGCWC 1 cut(s) 344
BsiSI CCGG 4 cut(s) 197, 207, 345, 366
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 295
BsmBI CGTCTC 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 6 cut(s) 190, 200, 314, 348, 369, 388
Bsp119I TTCGAA 1 cut(s) 63
Bsp1286I GDGCHC 2 cut(s) 154, 344
Bsp19I CCATGG 1 cut(s) 292
BspACI CCGC 1 cut(s) 162
BspANI GGCC 6 cut(s) 190, 200, 314, 348, 369, 388
BspFNI CGCG 1 cut(s) 164
BspT104I TTCGAA 1 cut(s) 63
BsrDI GCAATG 1 cut(s) 389
BssECI CCNNGG 3 cut(s) 185, 292, 423
BssT1I CCWWGG 2 cut(s) 185, 292
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 2 cut(s) 306, 465
Bst6I CTCTTC 1 cut(s) 131
BstBI TTCGAA 1 cut(s) 63
BstDSI CCRYGG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 164
BstHHI GCGC 1 cut(s) 166
BstMAI GTCTC 1 cut(s) 295
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 423
BstSLI GKGCMC 1 cut(s) 154
BstUI CGCG 1 cut(s) 164
BsuRI GGCC 6 cut(s) 190, 200, 314, 348, 369, 388
BtgI CCRYGG 1 cut(s) 292
CfoI GCGC 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 458
Csp6I GTAC 2 cut(s) 35, 45
CspCI CAANNNNNGTGG 6 cut(s) 190, 225, 268, 303, 304, 339
CviAII CATG 1 cut(s) 293
CviQI GTAC 2 cut(s) 35, 45
EaeI YGGCCR 4 cut(s) 198, 346, 367, 386
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 2 cut(s) 185, 292
Eco47I GGWCC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 423
EcoT14I CCWWGG 2 cut(s) 185, 292
ErhI CCWWGG 2 cut(s) 185, 292
Esp3I CGTCTC 1 cut(s) 295
FaeI CATG 1 cut(s) 296
FaiI YATR 4 cut(s) 24, 158, 294, 299
FaqI GGGAC 1 cut(s) 20
FatI CATG 1 cut(s) 292
FauI CCCGC 1 cut(s) 169
GlaI GCGC 1 cut(s) 165
HaeIII GGCC 6 cut(s) 190, 200, 314, 348, 369, 388
HapII CCGG 4 cut(s) 197, 207, 345, 366
HhaI GCGC 1 cut(s) 166
Hin1II CATG 1 cut(s) 296
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 1 cut(s) 193
HpaII CCGG 4 cut(s) 197, 207, 345, 366
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 2 cut(s) 91, 325
Hpy188III TCNNGA 1 cut(s) 239
Hpy8I GTNNAC 2 cut(s) 91, 325
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 2 cut(s) 306, 465
HpyCH4IV ACGT 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpySE526I ACGT 1 cut(s) 59
Hsp92II CATG 1 cut(s) 296
HspAI GCGC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 347, 427
LpnPI CCDG 7 cut(s) 117, 210, 220, 358, 379, 410, 437
LweI GCATC 2 cut(s) 212, 381
MaeII ACGT 1 cut(s) 59
MboII GAAGA 2 cut(s) 124, 148
MhlI GDGCHC 2 cut(s) 154, 344
MluCI AATT 1 cut(s) 66
MmeI TCCRAC 1 cut(s) 436
MnlI CCTC 5 cut(s) 38, 89, 93, 266, 304
MseI TTAA 1 cut(s) 18
MspI CCGG 4 cut(s) 197, 207, 345, 366
MspR9I CCNGG 1 cut(s) 425
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 164
MwoI GCNNNNNNNGC 1 cut(s) 149
NcoI CCATGG 1 cut(s) 292
NlaIII CATG 1 cut(s) 296
NspV TTCGAA 1 cut(s) 63
PfeI GAWTC 1 cut(s) 193
PflMI CCANNNNNTGG 1 cut(s) 401
Psp1406I AACGTT 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 423
PspGI CCWGG 1 cut(s) 423
PspPI GGNCC 1 cut(s) 458
RsaI GTAC 2 cut(s) 36, 46
RsaNI GTAC 2 cut(s) 35, 45
SaqAI TTAA 1 cut(s) 18
Sau96I GGNCC 1 cut(s) 458
ScaI AGTACT 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 2 cut(s) 154, 344
SetI ASST 7 cut(s) 62, 83, 145, 183, 247, 284, 447
SfaNI GCATC 2 cut(s) 212, 381
SfuI TTCGAA 1 cut(s) 63
SinI GGWCC 1 cut(s) 458
Sse9I AATT 1 cut(s) 66
SsiI CCGC 1 cut(s) 162
StyD4I CCNGG 1 cut(s) 423
StyI CCWWGG 2 cut(s) 185, 292
TaaI ACNGT 2 cut(s) 306, 465
TaiI ACGT 1 cut(s) 62
TaqI TCGA 3 cut(s) 63, 220, 358
TasI AATT 1 cut(s) 66
TatI WGTACW 1 cut(s) 34
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TspGWI ACGGA 1 cut(s) 108
Van91I CCANNNNNTGG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 458
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.