pycom12g17710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014830
Physical Location & Seq
Reverse (-)
20138435 .. 20138925
491 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g17710.2

Sequence Viewer

Length: 405 bp
ATGGACAAGAAGAAGGGAAGCTGCAGCCAATCAGAAAGAAAGAAGAAAAAAGAAAAAAAAAAAAAAAAAAGGGGGAAAGCTTCTCGTGCACCCATACCCGCACCACCGCGAGTCCATCTTCCAAGGCCGATTCCGGCCAACTCCGGTGGATTTTTTGGATGCCATCACCACCATCTTGAAGCTCTCATTCCCCTCTACAAAACCCACCCAAGAACCACCTTGATTAACCATGGTATGGGGCGGTTTGAGGCCACGAAAGTTGACGAAAATCAATGGTGCTCCGGCCACCCTTTTCGATTTTCCGGCAAATCCGCAAAGCGATGGCTGATGCCACCACTTGGGCTTTGTAGCCCATCATCCCAGGAGAAAAACCCAACCAACCTTGACCCCGTTGGACCACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.99

Weight (kDa)

10.33

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 235, 338
AccII CGCG 1 cut(s) 109
AciI CCGC 4 cut(s) 99, 107, 241, 312
AcoI YGGCCR 2 cut(s) 135, 283
AfiI CCNNNNNNNGG 2 cut(s) 235, 338
AgsI TTSAA 1 cut(s) 179
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 3 cut(s) 21, 80, 182
AluI AGCT 3 cut(s) 21, 80, 182
Alw21I GWGCWC 2 cut(s) 91, 281
Alw44I GTGCAC 1 cut(s) 87
AoxI GGCC 4 cut(s) 125, 135, 249, 283
ApaLI GTGCAC 1 cut(s) 87
ApeKI GCWGC 2 cut(s) 21, 24
AspS9I GGNCC 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 158
AvaII GGWCC 1 cut(s) 395
BaeGI GKGCMC 1 cut(s) 91
BauI CACGAG 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 91, 281
BbvI GCAGC 2 cut(s) 8, 36
BccI CCATC 5 cut(s) 123, 171, 180, 315, 361
BciT130I CCWGG 1 cut(s) 362
BfmI CTRYAG 1 cut(s) 22
BisI GCNGC 2 cut(s) 22, 25
BlsI GCNGC 2 cut(s) 23, 26
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 395
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 2 cut(s) 149, 318
BsaJI CCNNGG 3 cut(s) 122, 229, 360
BsaWI WCCGGW 1 cut(s) 143
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 338
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 122, 229, 360
BseGI GGATG 2 cut(s) 164, 356
BseLI CCNNNNNNNGG 2 cut(s) 235, 338
BseSI GKGCMC 1 cut(s) 91
BseXI GCAGC 2 cut(s) 8, 36
Bsh1236I CGCG 1 cut(s) 109
BshFI GGCC 4 cut(s) 127, 137, 251, 285
BsiHKAI GWGCWC 2 cut(s) 91, 281
BsiSI CCGG 4 cut(s) 134, 144, 282, 303
BslI CCNNNNNNNGG 2 cut(s) 235, 338
BsnI GGCC 4 cut(s) 127, 137, 251, 285
Bsp1286I GDGCHC 2 cut(s) 91, 281
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 4 cut(s) 99, 107, 241, 312
BspANI GGCC 4 cut(s) 127, 137, 251, 285
BspFNI CGCG 1 cut(s) 109
BspMAI CTGCAG 1 cut(s) 26
BssECI CCNNGG 3 cut(s) 122, 229, 360
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 2 cut(s) 122, 229
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 1 cut(s) 402
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 2 cut(s) 164, 356
BstFNI CGCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
BstSFI CTRYAG 1 cut(s) 22
BstSLI GKGCMC 1 cut(s) 91
BstUI CGCG 1 cut(s) 109
BstV1I GCAGC 2 cut(s) 8, 36
BsuRI GGCC 4 cut(s) 127, 137, 251, 285
BtgI CCRYGG 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 334
BtsCI GGATG 2 cut(s) 164, 356
Cfr13I GGNCC 1 cut(s) 395
CspCI CAANNNNNGTGG 4 cut(s) 127, 162, 241, 276
CviAII CATG 1 cut(s) 230
EaeI YGGCCR 2 cut(s) 135, 283
Eco130I CCWWGG 2 cut(s) 122, 229
Eco47I GGWCC 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 2 cut(s) 122, 229
ErhI CCWWGG 2 cut(s) 122, 229
FaeI CATG 1 cut(s) 233
FaiI YATR 3 cut(s) 95, 231, 236
FatI CATG 1 cut(s) 229
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 22, 25
FokI GGATG 2 cut(s) 171, 343
Fsp4HI GCNGC 2 cut(s) 22, 25
GluI GCNGC 2 cut(s) 22, 25
HaeIII GGCC 4 cut(s) 127, 137, 251, 285
HapII CCGG 4 cut(s) 134, 144, 282, 303
Hin1II CATG 1 cut(s) 233
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 2 cut(s) 111, 130
HpaII CCGG 4 cut(s) 134, 144, 282, 303
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 2 cut(s) 89, 262
Hpy188I TCNGA 1 cut(s) 34
Hpy188III TCNNGA 1 cut(s) 176
Hpy8I GTNNAC 2 cut(s) 89, 262
HpyAV CCTTC 1 cut(s) 7
HpyCH4III ACNGT 1 cut(s) 402
HpyCH4V TGCA 2 cut(s) 24, 89
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
Hsp92II CATG 1 cut(s) 233
LmnI GCTCC 1 cut(s) 284
LpnPI CCDG 6 cut(s) 147, 157, 295, 316, 347, 374
Lsp1109I GCAGC 2 cut(s) 8, 36
LweI GCATC 2 cut(s) 149, 318
MboII GAAGA 3 cut(s) 22, 55, 110
MhlI GDGCHC 2 cut(s) 91, 281
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 373
MnlI CCTC 2 cut(s) 203, 241
MseI TTAA 1 cut(s) 225
MspI CCGG 4 cut(s) 134, 144, 282, 303
MspR9I CCNGG 1 cut(s) 362
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 86
NcoI CCATGG 1 cut(s) 229
NlaIII CATG 1 cut(s) 233
PfeI GAWTC 1 cut(s) 130
PflMI CCANNNNNTGG 2 cut(s) 235, 338
PkrI GCNGC 2 cut(s) 23, 26
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspPI GGNCC 1 cut(s) 395
PstI CTGCAG 1 cut(s) 26
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 2 cut(s) 22, 25
Sau96I GGNCC 1 cut(s) 395
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 2 cut(s) 91, 281
SetI ASST 5 cut(s) 23, 82, 184, 221, 384
SfaNI GCATC 2 cut(s) 149, 318
SfcI CTRYAG 1 cut(s) 22
SinI GGWCC 1 cut(s) 395
SsiI CCGC 4 cut(s) 99, 107, 241, 312
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 2 cut(s) 122, 229
TaaI ACNGT 1 cut(s) 402
TaqI TCGA 1 cut(s) 295
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TseI GCWGC 2 cut(s) 21, 24
Van91I CCANNNNNTGG 2 cut(s) 235, 338
VneI GTGCAC 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.