pycom06g10120

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006747
Physical Location & Seq
Forward (+)
15496708 .. 15500207
3500 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1155 bp
ATGGACAGTGCATTCGAGGATGAGGCTGAGCACACGGTTTCGATCCAAGAGTATCTTAAGGATGTTGAGGAGCAGGAACTGGAAGCTGATTTGGTTTTGGGAGGCGATGAAGGCAAGGTGTGTACCTATAATAATGGTTATATGAAGAGGCAAGCAATTTTTTCATGTCTGACTTGCACCCCAGAAGGGAATGCTGGAGTTTGCACAGCTTGTAGCTTGTCTTGCCATGATGGCCATGAGATTGTAGAACTGTGGACCAAAAGATATTTTAGATGTGATTGTGGAAATTCAAAATTTGGAGAGTTCTTTTGCAAGCTTTCCCCCAATAAAGATGTAGAAAACGTTGAAAATTCTTACAATCAAAATTTCAAGGGATCATACTGCACCTGCAGTCGCCCCTATCCTGATCCAGATGTTGAAGATCAAGTAGAGATGATACAATGCTGTGTGTGTGAAGACTGGTTCCATGAGGAACACATCGGTCTTGACTCTTCTGATGAGATTCCAAGAGATGAGGAAGGTGAACCACTTTATGAAGATTTTATTTGCAAGGGATGCTCAGCAGTTTGCTGTTTTATCAGACTATACAATCAAAATATCTTGGCATCTGGCAGACGAAAGGATGCTCCCATCGAGTCTGCCAAGGATAAAGAGGTTTTGAGCAATATGCCTTTACCTTGTGGATCTAGAAAGCTAGAGAATGATATTTCTCAAAGTTGTAATACCATTTCTGAACCTGTATCCGGTGGGGATAGATTGCTACATGGGGAAAATTCTGGGAAGAATGCAGATGCGGGTCAATGCACTACAGATGCCAATCCACCTGTTACTTGTGTTCTTGGAGTTAATCTGGTGGTTGCTTCCCCTGTTTTAGAGTGTAAACCAATGTTTCTTTCTAAAAACTGGAGGGATGGACTCTGCAAGTGTGAAAAATGCAGTGAGTTTTACGTACAGAAGCATATTAGGTTTCTGCTTGACAAGGAAGACTCGATCATGGAGTATGAGAAAATGGCAAAGCTGAAGAGAGAGGAAAAATTGCAGGAACAGGAGGGGGCTGAGTTGACTATGCTCGATAAACTTGGCCATGTAGAGAAAATGGAGATTCTGAGTGGCATTGCAGACATGAAAGATGAGCTTCATTCTTTCCTGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-UBR

Protein Analysis

385

Amino Acids

43.17

Weight (kDa)

4.63

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBR PF02207 41 - 106 4.9e-13 Putative zinc finger in N-recognin (UBR box)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 395
Acc36I ACCTGC 1 cut(s) 395
AciI CCGC 1 cut(s) 794
AclI AACGTT 1 cut(s) 342
AclWI GGATC 4 cut(s) 37, 382, 401, 691
AcoI YGGCCR 2 cut(s) 232, 1081
AcsI RAATTY 5 cut(s) 286, 293, 349, 364, 772
AcuI CTGAAG 1 cut(s) 1040
AfaI GTAC 2 cut(s) 124, 951
AfiI CCNNNNNNNGG 2 cut(s) 186, 743
AflII CTTAAG 1 cut(s) 56
AgsI TTSAA 4 cut(s) 291, 347, 370, 419
AjnI CCWGG 1 cut(s) 1146
AjuI GAANNNNNNNTTGG 2 cut(s) 877, 909
AluBI AGCT 7 cut(s) 86, 209, 216, 316, 694, 1018, 1135
AluI AGCT 7 cut(s) 86, 209, 216, 316, 694, 1018, 1135
Alw21I GWGCWC 1 cut(s) 33
AlwI GGATC 4 cut(s) 37, 382, 401, 691
AlwNI CAGNNNCTG 1 cut(s) 79
AoxI GGCC 2 cut(s) 232, 1081
ApoI RAATTY 5 cut(s) 286, 293, 349, 364, 772
ArsI GACNNNNNNTTYG 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 533
AvaII GGWCC 1 cut(s) 255
BalI TGGCCA 2 cut(s) 234, 1083
BbsI GAAGAC 2 cut(s) 462, 990
Bbv12I GWGCWC 1 cut(s) 33
BccI CCATC 3 cut(s) 224, 638, 905
BciT130I CCWGG 1 cut(s) 1148
BciVI GTATCC 1 cut(s) 751
BfaI CTAG 2 cut(s) 687, 695
BfmI CTRYAG 2 cut(s) 388, 807
BfrI CTTAAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 395
BfuI GTATCC 1 cut(s) 751
BglI GCCNNNNNGGC 1 cut(s) 231
BlpI GCTNAGC 2 cut(s) 27, 559
Bme1390I CCNGG 1 cut(s) 1148
Bme18I GGWCC 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 464
BmrFI CCNGG 1 cut(s) 1148
BmsI GCATC 5 cut(s) 545, 613, 614, 781, 802
BpiI GAAGAC 2 cut(s) 462, 990
BpmI CTGGAG 2 cut(s) 216, 925
Bpu1102I GCTNAGC 2 cut(s) 27, 559
BsaAI YACGTR 1 cut(s) 949
BsaBI GATNNNNATC 1 cut(s) 816
BsaJI CCNNGG 2 cut(s) 642, 1147
BsaWI WCCGGW 1 cut(s) 743
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 743
Bse1I ACTGG 3 cut(s) 84, 464, 908
Bse3DI GCAATG 1 cut(s) 1113
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 1 cut(s) 1148
BseDI CCNNGG 2 cut(s) 642, 1147
BseGI GGATG 5 cut(s) 25, 67, 560, 628, 916
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 2 cut(s) 186, 743
BseMI GCAATG 1 cut(s) 1113
BseMII CTCAG 4 cut(s) 18, 573, 1047, 1097
BseNI ACTGG 3 cut(s) 84, 464, 908
BseRI GAGGAG 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 367
BshFI GGCC 2 cut(s) 234, 1083
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 744
BslI CCNNNNNNNGG 2 cut(s) 186, 743
BsmI GAATGC 3 cut(s) 11, 196, 790
BsnI GGCC 2 cut(s) 234, 1083
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 6 cut(s) 42, 374, 406, 421, 683, 990
Bsp1720I GCTNAGC 2 cut(s) 27, 559
BspACI CCGC 1 cut(s) 794
BspANI GGCC 2 cut(s) 234, 1083
BspCNI CTCAG 4 cut(s) 19, 572, 1048, 1098
BspLI GGNNCC 1 cut(s) 464
BspMAI CTGCAG 1 cut(s) 392
BspMI ACCTGC 1 cut(s) 395
BspPI GGATC 4 cut(s) 37, 382, 401, 691
BspTI CTTAAG 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 1113
BsrI ACTGG 3 cut(s) 84, 464, 908
BssECI CCNNGG 2 cut(s) 642, 1147
BssMI GATC 6 cut(s) 42, 374, 406, 421, 683, 990
BssT1I CCWWGG 1 cut(s) 642
Bst2UI CCWGG 1 cut(s) 1148
Bst4CI ACNGT 3 cut(s) 8, 37, 252
Bst6I CTCTTC 3 cut(s) 140, 496, 1016
BstAFI CTTAAG 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 555
BstBAI YACGTR 1 cut(s) 949
BstC8I GCNNGC 2 cut(s) 153, 314
BstDEI CTNAG 4 cut(s) 27, 559, 1056, 1106
BstF5I GGATG 5 cut(s) 25, 67, 560, 628, 916
BstKTI GATC 6 cut(s) 45, 377, 409, 424, 686, 993
BstMBI GATC 6 cut(s) 42, 374, 406, 421, 683, 990
BstMWI GCNNNNNNNGC 4 cut(s) 111, 222, 231, 555
BstNI CCWGG 1 cut(s) 1148
BstSCI CCNGG 1 cut(s) 1146
BstSFI CTRYAG 2 cut(s) 388, 807
BstSNI TACGTA 1 cut(s) 949
BstV2I GAAGAC 2 cut(s) 462, 990
BstX2I RGATCY 1 cut(s) 683
BstYI RGATCY 1 cut(s) 683
BsuI GTATCC 1 cut(s) 751
BsuRI GGCC 2 cut(s) 234, 1083
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 5 cut(s) 25, 67, 560, 628, 916
BtsI GCAGTG 1 cut(s) 943
BtsIMutI CAGTG 2 cut(s) 13, 943
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 2 cut(s) 153, 314
CaiI CAGNNNCTG 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 255
Csp6I GTAC 2 cut(s) 123, 950
CviAII CATG 8 cut(s) 165, 227, 236, 467, 764, 994, 1085, 1123
CviQI GTAC 2 cut(s) 123, 950
DdeI CTNAG 4 cut(s) 27, 559, 1056, 1106
DpnI GATC 6 cut(s) 44, 376, 408, 423, 685, 992
DpnII GATC 6 cut(s) 42, 374, 406, 421, 683, 990
EaeI YGGCCR 2 cut(s) 232, 1081
Eam1104I CTCTTC 3 cut(s) 140, 496, 1016
EarI CTCTTC 3 cut(s) 140, 496, 1016
Eco105I TACGTA 1 cut(s) 949
Eco130I CCWWGG 1 cut(s) 642
Eco47I GGWCC 1 cut(s) 255
Eco57I CTGAAG 1 cut(s) 1040
EcoRII CCWGG 1 cut(s) 1146
EcoT14I CCWWGG 1 cut(s) 642
ErhI CCWWGG 1 cut(s) 642
FaeI CATG 8 cut(s) 168, 230, 239, 470, 767, 997, 1088, 1126
FalI AAGNNNNNCTT 4 cut(s) 39, 71, 1119, 1151
FatI CATG 8 cut(s) 164, 226, 235, 466, 763, 993, 1084, 1122
FauI CCCGC 1 cut(s) 787
FokI GGATG 5 cut(s) 32, 74, 567, 635, 923
FspBI CTAG 2 cut(s) 687, 695
GsuI CTGGAG 2 cut(s) 216, 925
HaeIII GGCC 2 cut(s) 234, 1083
HapII CCGG 1 cut(s) 744
Hin1II CATG 8 cut(s) 168, 230, 239, 470, 767, 997, 1088, 1126
HincII GTYRAC 1 cut(s) 1062
HindII GTYRAC 1 cut(s) 1062
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 6 cut(s) 488, 502, 635, 915, 986, 1102
HpaII CCGG 1 cut(s) 744
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 5 cut(s) 123, 255, 524, 881, 1062
Hpy188I TCNGA 5 cut(s) 171, 496, 581, 733, 1107
Hpy188III TCNNGA 4 cut(s) 404, 410, 485, 687
Hpy8I GTNNAC 5 cut(s) 123, 255, 524, 881, 1062
HpyAV CCTTC 3 cut(s) 104, 179, 512
HpyCH4III ACNGT 3 cut(s) 8, 37, 252
HpyCH4IV ACGT 2 cut(s) 342, 948
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 222, 231, 555
HpyF3I CTNAG 4 cut(s) 27, 559, 1056, 1106
HpySE526I ACGT 2 cut(s) 342, 948
Hsp92II CATG 8 cut(s) 168, 230, 239, 470, 767, 997, 1088, 1126
Kzo9I GATC 6 cut(s) 42, 374, 406, 421, 683, 990
LmnI GCTCC 2 cut(s) 70, 631
LweI GCATC 5 cut(s) 545, 613, 614, 781, 802
MaeI CTAG 2 cut(s) 687, 695
MaeII ACGT 2 cut(s) 342, 948
MaeIII GTNAC 1 cut(s) 826
MalI GATC 6 cut(s) 44, 376, 408, 423, 685, 992
MboI GATC 6 cut(s) 42, 374, 406, 421, 683, 990
MboII GAAGA 8 cut(s) 157, 431, 467, 483, 548, 793, 995, 1033
MflI RGATCY 1 cut(s) 683
MhlI GDGCHC 1 cut(s) 33
MlsI TGGCCA 2 cut(s) 234, 1083
MluCI AATT 7 cut(s) 156, 286, 293, 349, 364, 772, 1034
MluNI TGGCCA 2 cut(s) 234, 1083
MlyI GAGTC 4 cut(s) 482, 644, 909, 980
Mox20I TGGCCA 2 cut(s) 234, 1083
MscI TGGCCA 2 cut(s) 234, 1083
MseI TTAA 2 cut(s) 57, 846
Msp20I TGGCCA 2 cut(s) 234, 1083
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 1 cut(s) 744
MspR9I CCNGG 1 cut(s) 1148
Mva1269I GAATGC 3 cut(s) 11, 196, 790
MvaI CCWGG 1 cut(s) 1148
MwoI GCNNNNNNNGC 4 cut(s) 111, 222, 231, 555
NdeII GATC 6 cut(s) 42, 374, 406, 421, 683, 990
NlaIII CATG 8 cut(s) 168, 230, 239, 470, 767, 997, 1088, 1126
NlaIV GGNNCC 1 cut(s) 464
PaqCI CACCTGC 1 cut(s) 395
PctI GAATGC 3 cut(s) 11, 196, 790
PfeI GAWTC 2 cut(s) 502, 1102
PleI GAGTC 4 cut(s) 482, 643, 909, 980
PpsI GAGTC 4 cut(s) 482, 643, 909, 980
Ppu21I YACGTR 1 cut(s) 949
Psp1406I AACGTT 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 1146
PspGI CCWGG 1 cut(s) 1146
PspN4I GGNNCC 1 cut(s) 464
PspPI GGNCC 1 cut(s) 255
PstI CTGCAG 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 79
PsuI RGATCY 1 cut(s) 683
RsaI GTAC 2 cut(s) 124, 951
RsaNI GTAC 2 cut(s) 123, 950
SaqAI TTAA 2 cut(s) 57, 846
Sau3AI GATC 6 cut(s) 42, 374, 406, 421, 683, 990
Sau96I GGNCC 1 cut(s) 255
SchI GAGTC 4 cut(s) 482, 644, 909, 980
ScrFI CCNGG 1 cut(s) 1148
SduI GDGCHC 1 cut(s) 33
SfaNI GCATC 5 cut(s) 545, 613, 614, 781, 802
SfcI CTRYAG 2 cut(s) 388, 807
SinI GGWCC 1 cut(s) 255
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SnaBI TACGTA 1 cut(s) 949
Sse9I AATT 7 cut(s) 156, 286, 293, 349, 364, 772, 1034
SsiI CCGC 1 cut(s) 794
SspMI CTAG 2 cut(s) 687, 695
StyD4I CCNGG 1 cut(s) 1146
StyI CCWWGG 1 cut(s) 642
TaaI ACNGT 3 cut(s) 8, 37, 252
TaiI ACGT 2 cut(s) 345, 951
TaqI TCGA 5 cut(s) 15, 41, 633, 989, 1071
TaqII GACCGA 1 cut(s) 470
TasI AATT 7 cut(s) 156, 286, 293, 349, 364, 772, 1034
TfiI GAWTC 2 cut(s) 502, 1102
Tru1I TTAA 2 cut(s) 57, 846
Tru9I TTAA 2 cut(s) 57, 846
TscAI CASTG 2 cut(s) 13, 943
TspDTI ATGAA 6 cut(s) 123, 153, 158, 549, 1127, 1139
TspRI CASTG 2 cut(s) 13, 943
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 255
XapI RAATTY 5 cut(s) 286, 293, 349, 364, 772
XbaI TCTAGA 1 cut(s) 686
XspI CTAG 2 cut(s) 687, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.