pycom06g10930

HpcH/HpaI aldolase/citrate lyase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012383
Physical Location & Seq
Reverse (-)
16134503 .. 16135582
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1080 bp
ATGGCCACACTGACTTACCCCGCCGCCGCCGCCACAGTCAGAGCCACCAAATCAACAACCCCACCAGCTCTCAGGAAAACCATCTCCGTCATCCCAATCAAACCCAATTCCATCTCCCCACTCAAAACCCTAAACCGCACCTCCAAACCCACCACCTTCTCAATCAAATCCACCGCCTCCTCCGACCACTCCTCCACCGCCACAACCACAATCACCACCACCACCTCCATCTCCCCCTCCCCACTAACCCTCAAGTCCCGCCTCCAAAATGGCGATACCCTATATGGCCTCTTCCTCCTCTCCTTCTCCCCTACCCTCGCCGAGATTGCTGGCCTCTCCGGCTACGACTTCGTCGTCGTCGACATGGAGCACGGCCACGGTGGCATACCCGGAGCCCTCTCCTGCCTCCACGCCTTGGCCGCCACCCAAACCCCCGCCATTCTTCGCTTGCCCGAGACCTCCGCAACTTGGGCCAAGAAAGCCCTAGATCTGGGCCCACAAGGCATCATGTTCCCCATGATCGAATCCTCCAAGGCCGCCAAGGAGGCCGTCTCCTACTGCCGCTTCCCTCCTGCCGGCGTCCGCGGATCCGCCCATACCGTCGTTCGCGCCTCCAGCTACGGCATCGACGAGGGGTATTTGAGTAATTACGAGAACCAGCTGCTGATCATGTGCCAGGTGGAGAGCGAGGAGGGCGTGAAGCACGCGGAGGACATCGCGACCGTTGATGGGATCGACTGTATTCAGATGGGACCGTTGGATCTGAGCGCCAGCTTGGGATACCTGTGGGACCCAGCGAACAAGAAGGTGAGGAAAATGATGGAGGTGGCCGAGAAGGCAGTGCTGGGGTCCGACCCGAAAGAGGGCGGGGCCTACTTGGCCGGGTTCGCTATGCCCCACGATGGGCCTCTCGATTTGGGGACACGTGGATACCACATGGTGTCCGGAGCCGTTGATGTTGGGCTGTTTAGGGGCGCGGCGGTGGAGGACGTGAAGAGGTTCAAGATGAGCTTGATGGACGGTTTAGATGATGAGCAGGAGGATGAGAAGGATGCTGATGAGAAGTACTGGAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HpcH_HpaI

Protein Analysis

360

Amino Acids

38.28

Weight (kDa)

5.45

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HpcH_HpaI PF03328 95 - 324 6.1e-49 HpcH/HpaI aldolase/citrate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10750
fragaria_vesca FvH4_5g11021 FvH4_5g11021
malus_domestica MD06G1117400.v1.1
prunus_persica Prupe.5G128500_v2.0.a1
pyrus_communis pycom06g10930
rosa_chinensis RchiOBHm_Chr7g0186781
rosa_laevigata RLG00000004816
rosa_multiflora Rmu_co8215596.1_g000001 Rmu_sc0009734.1_g000001 Rmu_sc0040245.1_g000001 Rmu_ssc0000024.1_g000045
rosa_roxburghii Rroxscaffold_3G00267830
rosa_rugosa Rorug06G0476600
rosa_samantha Rh7AG063900 Rh7BG080400 Rh7CG083600 Rh7DG084500
rosa_wichuraiana Rw7G007250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
AccB7I CCANNNNNTGG 1 cut(s) 415
AccI GTMKAC 1 cut(s) 360
AccII CGCG 5 cut(s) 585, 609, 707, 719, 977
AccIII TCCGGA 1 cut(s) 944
AclWI GGATC 4 cut(s) 582, 595, 740, 768
AcoI YGGCCR 5 cut(s) 3, 373, 417, 828, 879
AcvI CACGTG 1 cut(s) 926
AcyI GRCGYC 1 cut(s) 579
AdeI CACNNNGTG 1 cut(s) 940
AfaI GTAC 1 cut(s) 1067
AfiI CCNNNNNNNGG 9 cut(s) 415, 468, 490, 575, 729, 862, 863, 902, 903
AflIII ACRYGT 1 cut(s) 923
AgsI TTSAA 1 cut(s) 1003
AjiI CACGTC 1 cut(s) 991
AjnI CCWGG 1 cut(s) 675
AluBI AGCT 5 cut(s) 68, 618, 661, 774, 1011
AluI AGCT 5 cut(s) 68, 618, 661, 774, 1011
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 2 cut(s) 449, 556
AlwI GGATC 4 cut(s) 582, 595, 740, 768
AlwNI CAGNNNCTG 1 cut(s) 664
Ama87I CYCGRG 1 cut(s) 452
Aor13HI TCCGGA 1 cut(s) 944
ApaI GGGCCC 1 cut(s) 497
ApeKI GCWGC 1 cut(s) 661
Asp700I GAANNNNTTC 1 cut(s) 998
AspLEI GCGC 3 cut(s) 611, 770, 977
AspS9I GGNCC 8 cut(s) 471, 493, 494, 752, 790, 849, 870, 905
AsuC2I CCSGG 2 cut(s) 390, 883
AsuHPI GGTGA 2 cut(s) 205, 820
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 3 cut(s) 752, 790, 849
BaeGI GKGCMC 1 cut(s) 497
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 587
BanII GRGCYC 2 cut(s) 397, 497
BbrPI CACGTG 1 cut(s) 926
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 648
BccI CCATC 8 cut(s) 89, 119, 236, 722, 742, 814, 896, 1009
BceAI ACGGC 4 cut(s) 388, 533, 637, 935
BciT130I CCWGG 1 cut(s) 677
BciVI GTATCC 2 cut(s) 773, 923
BclI TGATCA 1 cut(s) 666
BcnI CCSGG 2 cut(s) 390, 883
BcoDI GTCTC 2 cut(s) 449, 556
BfaI CTAG 1 cut(s) 485
BfoI RGCGCY 1 cut(s) 771
BfuI GTATCC 2 cut(s) 773, 923
BglI GCCNNNNNGGC 6 cut(s) 339, 381, 501, 545, 836, 878
BglII AGATCT 1 cut(s) 487
BisI GCNGC 8 cut(s) 24, 27, 30, 420, 537, 562, 662, 978
BlsI GCNGC 8 cut(s) 25, 28, 31, 421, 538, 563, 663, 979
BmcAI AGTACT 1 cut(s) 1067
Bme1390I CCNGG 3 cut(s) 390, 677, 883
Bme18I GGWCC 3 cut(s) 752, 790, 849
BmeT110I CYCGRG 1 cut(s) 452
BmgBI CACGTC 1 cut(s) 991
BmgT120I GGNCC 8 cut(s) 471, 493, 494, 752, 790, 849, 870, 905
BmiI GGNNCC 9 cut(s) 394, 495, 589, 753, 791, 792, 850, 871, 949
BmrFI CCNGG 3 cut(s) 390, 677, 883
BmsI GCATC 3 cut(s) 513, 633, 1042
BplI GAGNNNNNCTC 2 cut(s) 536, 568
BpmI CTGGAG 1 cut(s) 598
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 2 cut(s) 390, 883
BsaAI YACGTR 1 cut(s) 926
BsaHI GRCGYC 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 5 cut(s) 376, 414, 531, 540, 583
BsaWI WCCGGW 1 cut(s) 944
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 176, 206
Bsc4I CCNNNNNNNGG 9 cut(s) 415, 468, 490, 575, 729, 862, 863, 902, 903
Bse118I RCCGGY 1 cut(s) 575
Bse1I ACTGG 1 cut(s) 1073
BseAI TCCGGA 1 cut(s) 944
BseBI CCWGG 1 cut(s) 677
BseDI CCNNGG 5 cut(s) 376, 414, 531, 540, 583
BseGI GGATG 3 cut(s) 90, 1048, 1057
BseLI CCNNNNNNNGG 9 cut(s) 415, 468, 490, 575, 729, 862, 863, 902, 903
BseMII CTCAG 2 cut(s) 85, 755
BseNI ACTGG 1 cut(s) 1073
BseRI GAGGAG 4 cut(s) 169, 181, 287, 704
BseSI GKGCMC 1 cut(s) 497
BseXI GCAGC 1 cut(s) 648
BseYI CCCAGC 2 cut(s) 793, 844
Bsh1236I CGCG 5 cut(s) 585, 609, 707, 719, 977
Bsh1285I CGRYCG 1 cut(s) 723
BsiEI CGRYCG 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 5 cut(s) 339, 390, 576, 882, 945
BslFI GGGAC 4 cut(s) 241, 765, 803, 934
BslI CCNNNNNNNGG 9 cut(s) 415, 468, 490, 575, 729, 862, 863, 902, 903
BsmAI GTCTC 2 cut(s) 449, 556
BsmBI CGTCTC 1 cut(s) 556
BsmFI GGGAC 4 cut(s) 241, 765, 803, 934
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 452
Bsp120I GGGCCC 1 cut(s) 493
Bsp1286I GDGCHC 3 cut(s) 372, 397, 497
Bsp13I TCCGGA 1 cut(s) 944
Bsp143I GATC 6 cut(s) 487, 519, 587, 666, 732, 760
Bsp68I TCGCGA 1 cut(s) 719
BspCNI CTCAG 2 cut(s) 84, 756
BspEI TCCGGA 1 cut(s) 944
BspFNI CGCG 5 cut(s) 585, 609, 707, 719, 977
BspLI GGNNCC 9 cut(s) 394, 495, 589, 753, 791, 792, 850, 871, 949
BspPI GGATC 4 cut(s) 582, 595, 740, 768
BspTNI GGTCTC 1 cut(s) 449
BsrFI RCCGGY 1 cut(s) 575
BsrI ACTGG 1 cut(s) 1073
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 5 cut(s) 376, 414, 531, 540, 583
BssMI GATC 6 cut(s) 487, 519, 587, 666, 732, 760
BssNI GRCGYC 1 cut(s) 579
BssT1I CCWWGG 3 cut(s) 414, 531, 540
Bst2UI CCWGG 1 cut(s) 677
Bst4CI ACNGT 7 cut(s) 37, 380, 601, 724, 740, 756, 1022
Bst6I CTCTTC 2 cut(s) 296, 989
BstACI GRCGYC 1 cut(s) 579
BstBAI YACGTR 1 cut(s) 926
BstC8I GCNNGC 5 cut(s) 331, 449, 577, 705, 772
BstDEI CTNAG 2 cut(s) 71, 764
BstDSI CCRYGG 2 cut(s) 376, 583
BstF5I GGATG 3 cut(s) 90, 1048, 1057
BstFNI CGCG 5 cut(s) 585, 609, 707, 719, 977
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 3 cut(s) 611, 770, 977
BstKTI GATC 6 cut(s) 490, 522, 590, 669, 735, 763
BstMAI GTCTC 2 cut(s) 449, 556
BstMBI GATC 6 cut(s) 487, 519, 587, 666, 732, 760
BstMCI CGRYCG 1 cut(s) 723
BstNI CCWGG 1 cut(s) 677
BstSCI CCNGG 3 cut(s) 388, 675, 881
BstSLI GKGCMC 1 cut(s) 497
BstUI CGCG 5 cut(s) 585, 609, 707, 719, 977
BstV1I GCAGC 1 cut(s) 648
BstX2I RGATCY 3 cut(s) 487, 587, 760
BstYI RGATCY 3 cut(s) 487, 587, 760
BsuI GTATCC 2 cut(s) 773, 923
BtgI CCRYGG 2 cut(s) 376, 583
BtgZI GCGATG 1 cut(s) 700
BtrI CACGTC 1 cut(s) 991
BtsCI GGATG 3 cut(s) 90, 1048, 1057
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 2 cut(s) 8, 846
BtuMI TCGCGA 1 cut(s) 719
Cac8I GCNNGC 5 cut(s) 331, 449, 577, 705, 772
CaiI CAGNNNCTG 1 cut(s) 664
CfoI GCGC 3 cut(s) 611, 770, 977
Cfr10I RCCGGY 1 cut(s) 575
Cfr13I GGNCC 8 cut(s) 471, 493, 494, 752, 790, 849, 870, 905
Cfr42I CCGCGG 1 cut(s) 586
CseI GACGC 1 cut(s) 568
Csp6I GTAC 1 cut(s) 1066
CviAII CATG 5 cut(s) 364, 508, 517, 670, 937
CviQI GTAC 1 cut(s) 1066
DdeI CTNAG 2 cut(s) 71, 764
DpnI GATC 6 cut(s) 489, 521, 589, 668, 734, 762
DpnII GATC 6 cut(s) 487, 519, 587, 666, 732, 760
DraIII CACNNNGTG 1 cut(s) 940
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 5 cut(s) 3, 373, 417, 828, 879
Eam1104I CTCTTC 2 cut(s) 296, 989
EarI CTCTTC 2 cut(s) 296, 989
EciI GGCGGA 1 cut(s) 580
Eco130I CCWWGG 3 cut(s) 414, 531, 540
Eco24I GRGCYC 2 cut(s) 397, 497
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 3 cut(s) 752, 790, 849
Eco72I CACGTG 1 cut(s) 926
Eco88I CYCGRG 1 cut(s) 452
EcoO109I RGGNCCY 2 cut(s) 790, 870
EcoRII CCWGG 1 cut(s) 675
EcoT14I CCWWGG 3 cut(s) 414, 531, 540
EcoT38I GRGCYC 2 cut(s) 397, 497
ErhI CCWWGG 3 cut(s) 414, 531, 540
Esp3I CGTCTC 1 cut(s) 556
FaeI CATG 5 cut(s) 367, 511, 520, 673, 940
FalI AAGNNNNNCTT 2 cut(s) 995, 1027
FaqI GGGAC 4 cut(s) 241, 765, 803, 934
FatI CATG 5 cut(s) 363, 507, 516, 669, 936
FauI CCCGC 4 cut(s) 28, 266, 442, 860
FbaI TGATCA 1 cut(s) 666
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 8 cut(s) 24, 27, 30, 420, 537, 562, 662, 978
FokI GGATG 3 cut(s) 77, 1055, 1064
FriOI GRGCYC 2 cut(s) 397, 497
Fsp4HI GCNGC 8 cut(s) 24, 27, 30, 420, 537, 562, 662, 978
FspBI CTAG 1 cut(s) 485
GlaI GCGC 3 cut(s) 610, 769, 976
GluI GCNGC 8 cut(s) 24, 27, 30, 420, 537, 562, 662, 978
GsaI CCCAGC 2 cut(s) 797, 848
GsuI CTGGAG 1 cut(s) 598
HaeII RGCGCY 1 cut(s) 771
HapII CCGG 5 cut(s) 339, 390, 576, 882, 945
HgaI GACGC 1 cut(s) 568
HhaI GCGC 3 cut(s) 611, 770, 977
Hin1I GRCGYC 1 cut(s) 579
Hin1II CATG 5 cut(s) 367, 511, 520, 673, 940
Hin6I GCGC 3 cut(s) 609, 768, 975
HinP1I GCGC 3 cut(s) 609, 768, 975
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 1 cut(s) 524
HpaII CCGG 5 cut(s) 339, 390, 576, 882, 945
HphI GGTGA 2 cut(s) 205, 820
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 5 cut(s) 41, 184, 747, 765, 853
Hpy188III TCNNGA 5 cut(s) 73, 718, 911, 945, 1003
Hpy8I GTNNAC 1 cut(s) 361
Hpy99I CGWCG 5 cut(s) 356, 359, 362, 605, 632
HpyAV CCTTC 5 cut(s) 166, 313, 799, 829, 1042
HpyCH4III ACNGT 7 cut(s) 37, 380, 601, 724, 740, 756, 1022
HpyCH4IV ACGT 2 cut(s) 925, 990
HpyF3I CTNAG 2 cut(s) 71, 764
HpySE526I ACGT 2 cut(s) 925, 990
Hsp92I GRCGYC 1 cut(s) 579
Hsp92II CATG 5 cut(s) 367, 511, 520, 673, 940
HspAI GCGC 3 cut(s) 609, 768, 975
KflI GGGWCCC 1 cut(s) 790
Kpn2I TCCGGA 1 cut(s) 944
KroI GCCGGC 1 cut(s) 575
KroNI GCCGGC 1 cut(s) 577
Ksp22I TGATCA 1 cut(s) 666
KspI CCGCGG 1 cut(s) 586
Kzo9I GATC 6 cut(s) 487, 519, 587, 666, 732, 760
LmnI GCTCC 3 cut(s) 367, 392, 947
Lsp1109I GCAGC 1 cut(s) 648
LweI GCATC 3 cut(s) 513, 633, 1042
MaeI CTAG 1 cut(s) 485
MaeII ACGT 2 cut(s) 925, 990
MalI GATC 6 cut(s) 489, 521, 589, 668, 734, 762
MboI GATC 6 cut(s) 487, 519, 587, 666, 732, 760
MboII GAAGA 3 cut(s) 283, 434, 1006
MflI RGATCY 3 cut(s) 487, 587, 760
MhlI GDGCHC 3 cut(s) 372, 397, 497
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 106, 646
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 207, 738, 876
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 944
MroNI GCCGGC 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 998
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 585, 661
MspI CCGG 5 cut(s) 339, 390, 576, 882, 945
MspR9I CCNGG 3 cut(s) 390, 677, 883
MvaI CCWGG 1 cut(s) 677
MvnI CGCG 5 cut(s) 585, 609, 707, 719, 977
NaeI GCCGGC 1 cut(s) 577
NciI CCSGG 2 cut(s) 390, 883
NdeII GATC 6 cut(s) 487, 519, 587, 666, 732, 760
NgoMIV GCCGGC 1 cut(s) 575
NlaIII CATG 5 cut(s) 367, 511, 520, 673, 940
NlaIV GGNNCC 9 cut(s) 394, 495, 589, 753, 791, 792, 850, 871, 949
NmeAIII GCCGAG 2 cut(s) 346, 856
NruI TCGCGA 1 cut(s) 719
PcsI WCGNNNNNNNCGW 3 cut(s) 351, 357, 627
PdiI GCCGGC 1 cut(s) 577
PdmI GAANNNNTTC 1 cut(s) 998
PfeI GAWTC 1 cut(s) 524
PflFI GACNNNGTC 1 cut(s) 350
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 8 cut(s) 25, 28, 31, 421, 538, 563, 663, 979
PmaCI CACGTG 1 cut(s) 926
PmlI CACGTG 1 cut(s) 926
Ppu21I YACGTR 1 cut(s) 926
PpuMI RGGWCCY 1 cut(s) 790
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 1 cut(s) 675
PspCI CACGTG 1 cut(s) 926
PspFI CCCAGC 2 cut(s) 793, 844
PspGI CCWGG 1 cut(s) 675
PspN4I GGNNCC 9 cut(s) 394, 495, 589, 753, 791, 792, 850, 871, 949
PspOMI GGGCCC 1 cut(s) 493
PspPI GGNCC 8 cut(s) 471, 493, 494, 752, 790, 849, 870, 905
PspPPI RGGWCCY 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 664
PsuI RGATCY 3 cut(s) 487, 587, 760
PsyI GACNNNGTC 1 cut(s) 350
PvuII CAGCTG 1 cut(s) 661
RruI TCGCGA 1 cut(s) 719
RsaI GTAC 1 cut(s) 1067
RsaNI GTAC 1 cut(s) 1066
SacII CCGCGG 1 cut(s) 586
SalI GTCGAC 1 cut(s) 359
SatI GCNGC 8 cut(s) 24, 27, 30, 420, 537, 562, 662, 978
Sau3AI GATC 6 cut(s) 487, 519, 587, 666, 732, 760
Sau96I GGNCC 8 cut(s) 471, 493, 494, 752, 790, 849, 870, 905
ScaI AGTACT 1 cut(s) 1067
ScrFI CCNGG 3 cut(s) 390, 677, 883
SduI GDGCHC 3 cut(s) 372, 397, 497
SfaNI GCATC 3 cut(s) 513, 633, 1042
SfiI GGCCNNNNNGGCC 1 cut(s) 878
Sfr303I CCGCGG 1 cut(s) 586
SgrBI CCGCGG 1 cut(s) 586
SinI GGWCC 3 cut(s) 752, 790, 849
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 2 cut(s) 106, 646
SspMI CTAG 1 cut(s) 485
StyD4I CCNGG 3 cut(s) 388, 675, 881
StyI CCWWGG 3 cut(s) 414, 531, 540
TaaI ACNGT 7 cut(s) 37, 380, 601, 724, 740, 756, 1022
TaiI ACGT 2 cut(s) 928, 993
TaqI TCGA 5 cut(s) 360, 522, 627, 735, 912
TasI AATT 2 cut(s) 106, 646
TatI WGTACW 1 cut(s) 1065
TauI GCSGC 7 cut(s) 26, 29, 32, 422, 539, 564, 980
TfiI GAWTC 1 cut(s) 524
TscAI CASTG 2 cut(s) 15, 846
TseI GCWGC 1 cut(s) 661
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 2 cut(s) 15, 846
Tth111I GACNNNGTC 1 cut(s) 350
Van91I CCANNNNNTGG 1 cut(s) 415
VpaK11BI GGWCC 3 cut(s) 752, 790, 849
XmiI GTMKAC 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 998
XspI CTAG 1 cut(s) 485
ZrmI AGTACT 1 cut(s) 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.