pycom06g11500

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013468
Physical Location & Seq
Reverse (-)
16800170 .. 16801481
1312 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
ATGGCAAACCTACTAAACAGAACATTTGGCTCTTTCATATCTTCCATTGCATGCTACTCTTACTTTAACAACAACGATCATCACCACCACCACCACCATCACTACCAAAGCTTCATCAACAATAAGAAAATCAAACACAGCATGCCAAGGGGTCGGCCGCTTTCGCTACAGACTGTGGAGCTCAAAGTCAGGATGTGCTGCACTGGCTGTGAAAGAGTGGTGAAAAATGCCATTTTCAAGCTCAGAGGGATTGATTCTGTGGATGTGAATCTACCATTGGAAAAGGTGACAGTAATTGGGTACGTTGATCGCAACAAAGTCCTCAAGGCAGTGAGGAGGGCTGGAAAAAGGGCCGAGTTCTGGCCCTACTCCAACCCTCCATTGTACTTCACATCTTCAAATGACTATTTTAAGGACACAACCAATGAATTCAAAGAGAGCTACAACTACTATAGGCATGGCTACAATGTTGGGGACAAACATGGGACCATTCCAGTGACTCAAAGGGGAGATGACAAAGTTAGCAACATGTTCAATGATGATAATGTCAATGCGTGTTGCCTTATGTAA

Protein Analysis

190

Amino Acids

21.94

Weight (kDa)

9.28

Isoelectric Point (pI)

26.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 62 - 116 3.5e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18196 AT2G18196
fragaria_vesca FvH4_5g10380 FvH4_5g10380
malus_domestica MD06G1124600.v1.1 MD14G1141600.v1.1
prunus_persica Prupe.5G135200_v2.0.a1
pyrus_communis pycom06g11500 pycom14g11670
rosa_chinensis RchiOBHm_Chr7g0187841
rosa_laevigata RLG00000004752
rosa_multiflora Rmu_sc0004483.1_g000045
rosa_roxburghii Rroxscaffold_3G00267010
rosa_rugosa Rorug06G0484700
rosa_samantha Rh7BG091600 Rh7CG090700 Rh7DG092400
rosa_wichuraiana Rw7G007760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 158
AcoI YGGCCR 1 cut(s) 155
AcsI RAATTY 1 cut(s) 428
AfaI GTAC 2 cut(s) 302, 386
AfiI CCNNNNNNNGG 1 cut(s) 360
AflIII ACRYGT 1 cut(s) 528
AgsI TTSAA 4 cut(s) 238, 399, 433, 535
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AluBI AGCT 4 cut(s) 111, 181, 241, 441
AluI AGCT 4 cut(s) 111, 181, 241, 441
Alw21I GWGCWC 1 cut(s) 183
AoxI GGCC 3 cut(s) 155, 351, 362
ApeKI GCWGC 1 cut(s) 198
ApoI RAATTY 1 cut(s) 428
AspS9I GGNCC 3 cut(s) 351, 363, 486
AsuHPI GGTGA 3 cut(s) 74, 232, 298
AvaII GGWCC 1 cut(s) 486
BanII GRGCYC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 185
BccI CCATC 1 cut(s) 105
BfmI CTRYAG 2 cut(s) 167, 451
BisI GCNGC 2 cut(s) 158, 199
BlsI GCNGC 2 cut(s) 159, 200
Bme18I GGWCC 1 cut(s) 486
BmgT120I GGNCC 3 cut(s) 351, 363, 486
BmiI GGNNCC 1 cut(s) 487
BpuEI CTTGAG 1 cut(s) 308
BsaBI GATNNNNATC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 1 cut(s) 360
Bse1I ACTGG 2 cut(s) 208, 494
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 2 cut(s) 198, 268
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 360
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 256
BseNI ACTGG 2 cut(s) 208, 494
BseRI GAGGAG 1 cut(s) 349
BseX3I CGGCCG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 185
BsgI GTGCAG 1 cut(s) 184
Bsh1285I CGRYCG 1 cut(s) 158
BshFI GGCC 3 cut(s) 157, 353, 364
BsiEI CGRYCG 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 183
BslFI GGGAC 2 cut(s) 488, 499
BslI CCNNNNNNNGG 1 cut(s) 360
BsmFI GGGAC 2 cut(s) 488, 499
BsnI GGCC 3 cut(s) 157, 353, 364
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 2 cut(s) 76, 307
BspACI CCGC 1 cut(s) 158
BspANI GGCC 3 cut(s) 157, 353, 364
BspCNI CTCAG 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 487
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 2 cut(s) 208, 494
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 2 cut(s) 76, 307
BssT1I CCWWGG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 175, 292
BstC8I GCNNGC 2 cut(s) 52, 143
BstDEI CTNAG 1 cut(s) 242
BstF5I GGATG 2 cut(s) 198, 268
BstKTI GATC 2 cut(s) 79, 310
BstMBI GATC 2 cut(s) 76, 307
BstMCI CGRYCG 1 cut(s) 158
BstMWI GCNNNNNNNGC 2 cut(s) 163, 204
BstNSI RCATGY 3 cut(s) 54, 145, 532
BstSFI CTRYAG 2 cut(s) 167, 451
BstV1I GCAGC 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 155
BsuRI GGCC 3 cut(s) 157, 353, 364
BtsCI GGATG 2 cut(s) 198, 268
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 3 cut(s) 201, 336, 501
Cac8I GCNNGC 2 cut(s) 52, 143
Cfr13I GGNCC 3 cut(s) 351, 363, 486
Csp6I GTAC 2 cut(s) 301, 385
CviAII CATG 5 cut(s) 51, 142, 458, 482, 529
CviQI GTAC 2 cut(s) 301, 385
DdeI CTNAG 1 cut(s) 242
DpnI GATC 2 cut(s) 78, 309
DpnII GATC 2 cut(s) 76, 307
EaeI YGGCCR 1 cut(s) 155
EagI CGGCCG 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 181
EclXI CGGCCG 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 486
Eco52I CGGCCG 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 181
EcoICRI GAGCTC 1 cut(s) 181
EcoRI GAATTC 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 146
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 5 cut(s) 54, 145, 461, 485, 532
FaiI YATR 8 cut(s) 38, 52, 143, 453, 459, 483, 530, 566
FaqI GGGAC 2 cut(s) 488, 499
FatI CATG 5 cut(s) 50, 141, 457, 481, 528
Fnu4HI GCNGC 2 cut(s) 158, 199
FokI GGATG 2 cut(s) 205, 275
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 2 cut(s) 158, 199
GluI GCNGC 2 cut(s) 158, 199
HaeIII GGCC 3 cut(s) 157, 353, 364
Hin1II CATG 5 cut(s) 54, 145, 461, 485, 532
HindIII AAGCTT 1 cut(s) 109
HinfI GANTC 3 cut(s) 254, 268, 499
HphI GGTGA 3 cut(s) 74, 232, 298
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 1 cut(s) 190
HpyCH4III ACNGT 2 cut(s) 175, 292
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 2 cut(s) 50, 201
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 204
HpyF3I CTNAG 1 cut(s) 242
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 5 cut(s) 54, 145, 461, 485, 532
Kzo9I GATC 2 cut(s) 76, 307
LmnI GCTCC 1 cut(s) 178
LpnPI CCDG 5 cut(s) 175, 189, 327, 346, 507
Lsp1109I GCAGC 1 cut(s) 185
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 286, 496
MalI GATC 2 cut(s) 78, 309
MboI GATC 2 cut(s) 76, 307
MboII GAAGA 2 cut(s) 33, 387
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 2 cut(s) 294, 428
MlyI GAGTC 1 cut(s) 493
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 5 cut(s) 239, 327, 330, 332, 387
MseI TTAA 2 cut(s) 66, 411
MslI CAYNNNNRTG 1 cut(s) 494
MwoI GCNNNNNNNGC 2 cut(s) 163, 204
NdeII GATC 2 cut(s) 76, 307
NlaIII CATG 5 cut(s) 54, 145, 461, 485, 532
NlaIV GGNNCC 1 cut(s) 487
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 2 cut(s) 286, 496
NspI RCATGY 3 cut(s) 54, 145, 532
PaeI GCATGC 2 cut(s) 54, 145
PciI ACATGT 1 cut(s) 528
PfeI GAWTC 2 cut(s) 254, 268
PkrI GCNGC 2 cut(s) 159, 200
PleI GAGTC 1 cut(s) 493
PpsI GAGTC 1 cut(s) 493
PscI ACATGT 1 cut(s) 528
Psp124BI GAGCTC 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 487
PspPI GGNCC 3 cut(s) 351, 363, 486
RsaI GTAC 2 cut(s) 302, 386
RsaNI GTAC 2 cut(s) 301, 385
RseI CAYNNNNRTG 1 cut(s) 494
SacI GAGCTC 1 cut(s) 183
SaqAI TTAA 2 cut(s) 66, 411
SatI GCNGC 2 cut(s) 158, 199
Sau3AI GATC 2 cut(s) 76, 307
Sau96I GGNCC 3 cut(s) 351, 363, 486
SchI GAGTC 1 cut(s) 493
SduI GDGCHC 1 cut(s) 183
SetI ASST 7 cut(s) 12, 113, 183, 243, 288, 306, 443
SfcI CTRYAG 2 cut(s) 167, 451
SinI GGWCC 1 cut(s) 486
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
SphI GCATGC 2 cut(s) 54, 145
Sse9I AATT 2 cut(s) 294, 428
SsiI CCGC 1 cut(s) 158
SstI GAGCTC 1 cut(s) 183
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 2 cut(s) 175, 292
TaiI ACGT 1 cut(s) 306
TasI AATT 2 cut(s) 294, 428
TatI WGTACW 1 cut(s) 384
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 2 cut(s) 254, 268
Tru1I TTAA 2 cut(s) 66, 411
Tru9I TTAA 2 cut(s) 66, 411
TscAI CASTG 3 cut(s) 208, 336, 501
TseFI GTSAC 2 cut(s) 286, 496
TseI GCWGC 1 cut(s) 198
Tsp45I GTSAC 2 cut(s) 286, 496
TspDTI ATGAA 3 cut(s) 25, 103, 441
TspRI CASTG 3 cut(s) 208, 336, 501
VpaK11BI GGWCC 1 cut(s) 486
XapI RAATTY 1 cut(s) 428
XceI RCATGY 3 cut(s) 54, 145, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.