pycom06g14490

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011559
Physical Location & Seq
Forward (+)
19214021 .. 19215905
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1134 bp
ATGTGGGGGTTCATATGCCTTCTGGTTTTTGGGCTCTACAGTGCCTATGCAATCCGGCTTTTGGCTGCTTTTCACTTCATCGATGGCCAAAGGTTCATCAGATACAGAGATCTCATGGGTTTTTTATTTGGCAGAGAGATGTTTTACACCACTTGGGTTTTCCAATTTTTGACCCTTCTACTTGGAAACATGGGTTTCATTCTACTTGGGGGAAGAGCGCTCAAGGAAATTAACTCCGAATTCAGCGACTCACCATTTCGGCTTCAGTATTTTATCGCCATCACCGGAGTGACCTACTTTGTATTTGCATTCTTCATTCCAACATTGTCTGCAATGAGAAGTTGGCTCGGACCATCTACCGTTATCACCTTCGCTTACATTGTCATACTCTTGGTGGTAGTGGTTAAAGATGGGAAAGCTAACACCAAGAGAGATTATGCGATCCATGGAAACGAAGCAGACAGAGTACTCAATGCCTTTGGTGCAATTGCTGCTATTATCGTGTGCAACTCCTCCGGATTGCTACTCGAAATACAGTCAACACTAAGAGCTCCTGCAGTAACAAATATGAGGAAAGCTTTATATCTGCAGTTTACTGTGGGACTGGTATTTTACTATGGAGTGAGCATAGTAGGATACTGGGCTTATGGCTCAACTGTATCCGAATACCTTCCTCAAGAGCTAAGCGGTCCTAAATCGGTTAAGGTTCTCATAAATGCCGCTGTTTTTCTACAATCCATAGTCTCCCAACATATGTTCGTGGCACCAATCCACGAGTTCCTCGATACCAAGTTCCTCAAACTCGATAAGAGCATGAACTCAAAAGGAAATTTGAAGCGCAGATTGTATGTTCGAGCCCTCCTCTTTGCAGTAAATACATTTGTAACAGCAGCTTTTCCTTTCATGGGGGACTTTGTAAACTTGTTTGGATCCTTTACACTCGTTCCTCTAACTTTCGTGTTCCCGAGCATGATTTTCATCAAGGTGAAGGGAAAGGCAGCTTCACTGGAGAAGATTTTATGGCATTGGTTCAACATCGTTATTTTTTCGCTCCTTGCCGTCGTCACCACAATTTCTGCCGTTCGATTGATAGTCAACAATGTTAAGGAATATAGTTTCTTTGCAAATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

378

Amino Acids

42.55

Weight (kDa)

9.71

Isoelectric Point (pI)

27.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 2 - 360 3.6e-55 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 763
AccIII TCCGGA 1 cut(s) 515
AciI CCGC 2 cut(s) 687, 720
AclWI GGATC 3 cut(s) 436, 924, 937
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 2 cut(s) 239, 829
AcuI CTGAAG 1 cut(s) 248
AfaI GTAC 1 cut(s) 468
AfeI AGCGCT 1 cut(s) 219
AfiI CCNNNNNNNGG 2 cut(s) 61, 905
AgsI TTSAA 2 cut(s) 835, 1033
AleI CACNNNNGTG 1 cut(s) 287
AluBI AGCT 6 cut(s) 419, 551, 578, 682, 893, 1001
AluI AGCT 6 cut(s) 419, 551, 578, 682, 893, 1001
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 748
AlwI GGATC 3 cut(s) 436, 924, 937
Ama87I CYCGRG 1 cut(s) 964
Aor13HI TCCGGA 1 cut(s) 515
Aor51HI AGCGCT 1 cut(s) 219
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 4 cut(s) 65, 491, 890, 998
ApoI RAATTY 2 cut(s) 239, 829
Asp700I GAANNNNTTC 1 cut(s) 669
AspLEI GCGC 2 cut(s) 220, 840
AspS9I GGNCC 2 cut(s) 350, 689
AsuHPI GGTGA 5 cut(s) 243, 274, 358, 997, 1057
AvaI CYCGRG 1 cut(s) 964
AvaII GGWCC 2 cut(s) 350, 689
BalI TGGCCA 1 cut(s) 87
BamHI GGATCC 1 cut(s) 929
BanI GGYRCC 1 cut(s) 763
BanII GRGCYC 3 cut(s) 36, 553, 859
BauI CACGAG 1 cut(s) 773
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 4 cut(s) 52, 478, 902, 1010
BccI CCATC 4 cut(s) 77, 287, 361, 404
BceAI ACGGC 2 cut(s) 1043, 1064
BciVI GTATCC 2 cut(s) 629, 670
BcoDI GTCTC 1 cut(s) 748
BfmI CTRYAG 3 cut(s) 37, 555, 587
BfoI RGCGCY 1 cut(s) 221
BfuI GTATCC 2 cut(s) 629, 670
BglII AGATCT 1 cut(s) 109
BisI GCNGC 5 cut(s) 66, 492, 720, 891, 999
BlpI GCTNAGC 1 cut(s) 683
BlsI GCNGC 5 cut(s) 67, 493, 721, 892, 1000
BmcAI AGTACT 1 cut(s) 468
Bme18I GGWCC 2 cut(s) 350, 689
BmeT110I CYCGRG 1 cut(s) 964
BmgT120I GGNCC 2 cut(s) 350, 689
BmiI GGNNCC 2 cut(s) 765, 931
BmrI ACTGGG 1 cut(s) 649
BmuI ACTGGG 1 cut(s) 649
BplI GAGNNNNNCTC 2 cut(s) 846, 878
BpmI CTGGAG 1 cut(s) 1028
Bpu1102I GCTNAGC 1 cut(s) 683
BpuEI CTTGAG 2 cut(s) 206, 660
Bsa29I ATCGAT 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 977
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 2 cut(s) 284, 515
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 905
Bse1I ACTGG 3 cut(s) 609, 644, 1011
Bse3DI GCAATG 1 cut(s) 339
Bse8I GATNNNNATC 1 cut(s) 977
BseAI TCCGGA 1 cut(s) 515
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 445
BseJI GATNNNNATC 1 cut(s) 977
BseLI CCNNNNNNNGG 2 cut(s) 61, 905
BseMI GCAATG 1 cut(s) 339
BseNI ACTGG 3 cut(s) 609, 644, 1011
BseRI GAGGAG 2 cut(s) 502, 851
BseXI GCAGC 4 cut(s) 52, 478, 902, 1010
BshFI GGCC 1 cut(s) 87
BshNI GGYRCC 1 cut(s) 763
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 964
BsiSI CCGG 3 cut(s) 55, 285, 516
BslFI GGGAC 2 cut(s) 615, 923
BslI CCNNNNNNNGG 2 cut(s) 61, 905
BsmAI GTCTC 1 cut(s) 748
BsmFI GGGAC 2 cut(s) 615, 923
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 1 cut(s) 87
BsoBI CYCGRG 1 cut(s) 964
Bsp1286I GDGCHC 3 cut(s) 36, 553, 859
Bsp13I TCCGGA 1 cut(s) 515
Bsp143I GATC 3 cut(s) 109, 441, 929
Bsp1720I GCTNAGC 1 cut(s) 683
Bsp19I CCATGG 1 cut(s) 445
BspACI CCGC 2 cut(s) 687, 720
BspANI GGCC 1 cut(s) 87
BspDI ATCGAT 1 cut(s) 81
BspEI TCCGGA 1 cut(s) 515
BspLI GGNNCC 2 cut(s) 765, 931
BspMAI CTGCAG 2 cut(s) 559, 591
BspPI GGATC 3 cut(s) 436, 924, 937
BspQI GCTCTTC 1 cut(s) 208
BspT107I GGYRCC 1 cut(s) 763
BsrDI GCAATG 1 cut(s) 339
BsrI ACTGG 3 cut(s) 609, 644, 1011
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 3 cut(s) 109, 441, 929
BssSI CACGAG 1 cut(s) 773
BssT1I CCWWGG 1 cut(s) 445
Bst2BI CACGAG 1 cut(s) 773
Bst4CI ACNGT 5 cut(s) 41, 361, 537, 598, 658
Bst6I CTCTTC 1 cut(s) 208
BstAPI GCANNNNNTGC 1 cut(s) 491
BstDEI CTNAG 2 cut(s) 545, 683
BstDSI CCRYGG 1 cut(s) 445
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 2 cut(s) 220, 840
BstKTI GATC 3 cut(s) 112, 444, 932
BstMAI GTCTC 1 cut(s) 748
BstMBI GATC 3 cut(s) 109, 441, 929
BstMWI GCNNNNNNNGC 2 cut(s) 482, 491
BstSFI CTRYAG 3 cut(s) 37, 555, 587
BstV1I GCAGC 4 cut(s) 52, 478, 902, 1010
BstX2I RGATCY 2 cut(s) 109, 929
BstYI RGATCY 2 cut(s) 109, 929
Bsu15I ATCGAT 1 cut(s) 81
BsuI GTATCC 2 cut(s) 629, 670
BsuRI GGCC 1 cut(s) 87
BsuTUI ATCGAT 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 445
BtsIMutI CAGTG 2 cut(s) 46, 1004
CfoI GCGC 2 cut(s) 220, 840
Cfr13I GGNCC 2 cut(s) 350, 689
ClaI ATCGAT 1 cut(s) 81
Csp6I GTAC 1 cut(s) 467
CviAII CATG 7 cut(s) 115, 190, 446, 814, 904, 970, 1131
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 2 cut(s) 545, 683
DpnI GATC 3 cut(s) 111, 443, 931
DpnII GATC 3 cut(s) 109, 441, 929
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 551
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 3 cut(s) 36, 553, 859
Eco47I GGWCC 2 cut(s) 350, 689
Eco47III AGCGCT 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 551
Eco57I CTGAAG 1 cut(s) 248
Eco88I CYCGRG 1 cut(s) 964
EcoICRI GAGCTC 1 cut(s) 551
EcoRI GAATTC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 3 cut(s) 36, 553, 859
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 7 cut(s) 118, 193, 449, 817, 907, 973, 1134
FaqI GGGAC 2 cut(s) 615, 923
FatI CATG 7 cut(s) 114, 189, 445, 813, 903, 969, 1130
FauNDI CATATG 2 cut(s) 14, 753
Fnu4HI GCNGC 5 cut(s) 66, 492, 720, 891, 999
FriOI GRGCYC 3 cut(s) 36, 553, 859
Fsp4HI GCNGC 5 cut(s) 66, 492, 720, 891, 999
GlaI GCGC 2 cut(s) 219, 839
GluI GCNGC 5 cut(s) 66, 492, 720, 891, 999
GsuI CTGGAG 1 cut(s) 1028
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 1 cut(s) 87
HapII CCGG 3 cut(s) 55, 285, 516
HhaI GCGC 2 cut(s) 220, 840
Hin1II CATG 7 cut(s) 118, 193, 449, 817, 907, 973, 1134
Hin6I GCGC 2 cut(s) 218, 838
HinP1I GCGC 2 cut(s) 218, 838
HincII GTYRAC 2 cut(s) 540, 1096
HindII GTYRAC 2 cut(s) 540, 1096
HindIII AAGCTT 1 cut(s) 576
HinfI GANTC 1 cut(s) 248
HpaII CCGG 3 cut(s) 55, 285, 516
HphI GGTGA 5 cut(s) 243, 274, 358, 997, 1057
Hpy166II GTNNAC 4 cut(s) 540, 594, 919, 1096
Hpy188I TCNGA 4 cut(s) 101, 238, 350, 664
Hpy188III TCNNGA 3 cut(s) 516, 677, 964
Hpy8I GTNNAC 4 cut(s) 540, 594, 919, 1096
Hpy99I CGWCG 1 cut(s) 1064
HpyAV CCTTC 5 cut(s) 29, 185, 379, 680, 982
HpyCH4III ACNGT 5 cut(s) 41, 361, 537, 598, 658
HpyCH4V TGCA 9 cut(s) 50, 308, 332, 485, 507, 557, 589, 869, 1124
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 491
HpyF3I CTNAG 2 cut(s) 545, 683
Hsp92II CATG 7 cut(s) 118, 193, 449, 817, 907, 973, 1134
HspAI GCGC 2 cut(s) 218, 838
Kpn2I TCCGGA 1 cut(s) 515
Kzo9I GATC 3 cut(s) 109, 441, 929
LguI GCTCTTC 1 cut(s) 208
LmnI GCTCC 2 cut(s) 556, 1056
LpnPI CCDG 8 cut(s) 8, 68, 298, 529, 567, 590, 625, 992
Lsp1109I GCAGC 4 cut(s) 52, 478, 902, 1010
MaeIII GTNAC 4 cut(s) 289, 559, 883, 1063
MalI GATC 3 cut(s) 111, 443, 931
MboI GATC 3 cut(s) 109, 441, 929
MboII GAAGA 3 cut(s) 225, 304, 1024
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 2 cut(s) 109, 929
MhlI GDGCHC 3 cut(s) 36, 553, 859
MlsI TGGCCA 1 cut(s) 87
MluCI AATT 6 cut(s) 164, 228, 239, 486, 829, 1071
MluNI TGGCCA 1 cut(s) 87
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 1 cut(s) 344
MnlI CCTC 8 cut(s) 523, 564, 684, 791, 806, 869, 872, 957
Mox20I TGGCCA 1 cut(s) 87
MroI TCCGGA 1 cut(s) 515
MroXI GAANNNNTTC 1 cut(s) 669
MscI TGGCCA 1 cut(s) 87
MseI TTAA 4 cut(s) 231, 405, 702, 1104
MslI CAYNNNNRTG 2 cut(s) 287, 983
Msp20I TGGCCA 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 3 cut(s) 55, 285, 516
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 1 cut(s) 308
MwoI GCNNNNNNNGC 2 cut(s) 482, 491
NcoI CCATGG 1 cut(s) 445
NdeI CATATG 2 cut(s) 14, 753
NdeII GATC 3 cut(s) 109, 441, 929
NlaIII CATG 7 cut(s) 118, 193, 449, 817, 907, 973, 1134
NlaIV GGNNCC 2 cut(s) 765, 931
NmuCI GTSAC 2 cut(s) 289, 1063
OliI CACNNNNGTG 1 cut(s) 287
PciSI GCTCTTC 1 cut(s) 208
PcsI WCGNNNNNNNCGW 1 cut(s) 780
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 669
PkrI GCNGC 5 cut(s) 67, 493, 721, 892, 1000
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
Psp124BI GAGCTC 1 cut(s) 553
PspN4I GGNNCC 2 cut(s) 765, 931
PspPI GGNCC 2 cut(s) 350, 689
PstI CTGCAG 2 cut(s) 559, 591
PsuI RGATCY 2 cut(s) 109, 929
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
RseI CAYNNNNRTG 2 cut(s) 287, 983
SacI GAGCTC 1 cut(s) 553
SapI GCTCTTC 1 cut(s) 208
SaqAI TTAA 4 cut(s) 231, 405, 702, 1104
SatI GCNGC 5 cut(s) 66, 492, 720, 891, 999
Sau3AI GATC 3 cut(s) 109, 441, 929
Sau96I GGNCC 2 cut(s) 350, 689
ScaI AGTACT 1 cut(s) 468
SchI GAGTC 1 cut(s) 242
SduI GDGCHC 3 cut(s) 36, 553, 859
SfcI CTRYAG 3 cut(s) 37, 555, 587
SinI GGWCC 2 cut(s) 350, 689
SmiMI CAYNNNNRTG 2 cut(s) 287, 983
SmlI CTYRAG 2 cut(s) 221, 675
SmoI CTYRAG 2 cut(s) 221, 675
Sse9I AATT 6 cut(s) 164, 228, 239, 486, 829, 1071
SsiI CCGC 2 cut(s) 687, 720
SstI GAGCTC 1 cut(s) 553
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 5 cut(s) 41, 361, 537, 598, 658
TaqI TCGA 6 cut(s) 81, 528, 783, 804, 853, 1084
TasI AATT 6 cut(s) 164, 228, 239, 486, 829, 1071
TatI WGTACW 1 cut(s) 466
TauI GCSGC 1 cut(s) 722
Tru1I TTAA 4 cut(s) 231, 405, 702, 1104
Tru9I TTAA 4 cut(s) 231, 405, 702, 1104
TscAI CASTG 2 cut(s) 46, 1011
TseFI GTSAC 2 cut(s) 289, 1063
TseI GCWGC 4 cut(s) 65, 491, 890, 998
Tsp45I GTSAC 2 cut(s) 289, 1063
TspDTI ATGAA 7 cut(s) 67, 85, 187, 304, 830, 892, 967
TspRI CASTG 2 cut(s) 46, 1011
VpaK11BI GGWCC 2 cut(s) 350, 689
XapI RAATTY 2 cut(s) 239, 829
XmnI GAANNNNTTC 1 cut(s) 669
ZrmI AGTACT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.