pycom06g20190

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005619
Physical Location & Seq
Forward (+)
23327033 .. 23329655
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGGGAAGTGTCTTGGATTCCAATTTCTTAGCTCTCACTGCCATTGTCACTGTGGGGTATCAGTTGTTGTTCTTCATAGTCACTGCTCTTCTCAAGTTCGACAAAGTCACTGACTTTGCTGGAAGTACCAATTTTGTTATACTTGCTATTCTGACCTTGGTTGTCAAAGGTTCATGGCATTTTAGACAGGTAGTGTTGAGTTTGCTTGTGATAATATGGGGTCTTCGCCTCGGATTTTTTCTACTGATGAGGATTTTGCAATGGGGAGAGGATCGGCGTTTTGATGAAATACGTGGTAATCTAGGGAAGTTAGCAGTTTTCTGGATATTTCAGGCTATGTGGGTATGGACTGTGAGTTTACCTGTAACAGTTGCTAATGCAAGCAACAGAACGCCATTGATCCAAGCTGCAGACATAATTGGTTGGATTATGTGGTCTGTTGGTTTTTCAGTTGAAGCTGCAGCTGATCAGCAAAAGTTAACTTTCAAAAGTTCTGCACAAAATAGAGGAAAGTGGTGCAATGTCGGACTATGGAAATACACTCGTCATCCAAACTATTTTGGTGAGATATTCCTTTGGTGGGGAGTATTTGTGGCTTCCACACCCGTATTGGAGGGTGCAGAGTGGCTGGTGATTCTTGGACCAATCTTTCTCACGTTGTTGCTTCTTTTCATCAGTGGCATCCCGTTGCTTGAGGAATCTACGGATAAGAAGTTTGGAAACATAGACGAATATAGGCTGTATAAAAGATCGACTAGCCCTCTGACACCGCTACCTCCAGTTATATACAAGAACTTGCCGTTGTGGTTCAAAACAACTTTTCTCTTTGAGTTTCCTCTCTACAGCCGTAATCTTCCTCGAGAAAAGCCTAACTGGAGAACAAGCCGGGTAGATCGAAGAAGCGATGGATTGAAGATCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1295

Protein Analysis

308

Amino Acids

35.22

Weight (kDa)

9.76

Isoelectric Point (pI)

26.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1295 PF06966 24 - 252 1.7e-87 Protein of unknown function (DUF1295)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 770
AclWI GGATC 2 cut(s) 279, 394
AfaI GTAC 1 cut(s) 127
AfiI CCNNNNNNNGG 1 cut(s) 580
AgsI TTSAA 4 cut(s) 455, 487, 811, 913
AluBI AGCT 4 cut(s) 32, 407, 458, 464
AluI AGCT 4 cut(s) 32, 407, 458, 464
AlwI GGATC 2 cut(s) 279, 394
Ama87I CYCGRG 1 cut(s) 858
ApeKI GCWGC 3 cut(s) 407, 458, 461
AspS9I GGNCC 1 cut(s) 641
AsuC2I CCSGG 1 cut(s) 887
AsuHPI GGTGA 2 cut(s) 575, 643
AvaI CYCGRG 1 cut(s) 858
AvaII GGWCC 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 3 cut(s) 394, 445, 473
BccI CCATC 1 cut(s) 899
BceAI ACGGC 2 cut(s) 784, 831
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 1 cut(s) 887
BfaI CTAG 3 cut(s) 302, 756, 922
BfmI CTRYAG 3 cut(s) 408, 459, 841
BisI GCNGC 3 cut(s) 408, 459, 462
BlsI GCNGC 3 cut(s) 409, 460, 463
Bme1390I CCNGG 1 cut(s) 887
Bme18I GGWCC 1 cut(s) 641
BmeT110I CYCGRG 1 cut(s) 858
BmgT120I GGNCC 1 cut(s) 641
BmrFI CCNGG 1 cut(s) 887
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 215
BpmI CTGGAG 2 cut(s) 762, 895
BpuEI CTTGAG 2 cut(s) 77, 713
BpuMI CCSGG 1 cut(s) 887
BsaAI YACGTR 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 156, 229
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 1 cut(s) 580
Bse1I ACTGG 2 cut(s) 779, 878
Bse3DI GCAATG 2 cut(s) 266, 526
BseDI CCNNGG 2 cut(s) 156, 229
BseGI GGATG 2 cut(s) 547, 681
BseLI CCNNNNNNNGG 1 cut(s) 580
BseMI GCAATG 2 cut(s) 266, 526
BseNI ACTGG 2 cut(s) 779, 878
BseXI GCAGC 3 cut(s) 394, 445, 473
BsgI GTGCAG 2 cut(s) 480, 639
BsiHKCI CYCGRG 1 cut(s) 858
BsiSI CCGG 1 cut(s) 886
BslI CCNNNNNNNGG 1 cut(s) 580
BsoBI CYCGRG 1 cut(s) 858
Bsp143I GATC 6 cut(s) 271, 399, 466, 749, 892, 915
BspACI CCGC 1 cut(s) 770
BspMAI CTGCAG 2 cut(s) 412, 463
BspPI GGATC 2 cut(s) 279, 394
BspQI GCTCTTC 1 cut(s) 93
BsrDI GCAATG 2 cut(s) 266, 526
BsrI ACTGG 2 cut(s) 779, 878
BssECI CCNNGG 2 cut(s) 156, 229
BssMI GATC 6 cut(s) 271, 399, 466, 749, 892, 915
BssT1I CCWWGG 1 cut(s) 156
Bst4CI ACNGT 3 cut(s) 52, 352, 370
Bst6I CTCTTC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 1 cut(s) 28
BstF5I GGATG 2 cut(s) 547, 681
BstKTI GATC 6 cut(s) 274, 402, 469, 752, 895, 918
BstMBI GATC 6 cut(s) 271, 399, 466, 749, 892, 915
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 885
BstSFI CTRYAG 3 cut(s) 408, 459, 841
BstV1I GCAGC 3 cut(s) 394, 445, 473
BstV2I GAAGAC 1 cut(s) 215
BtgZI GCGATG 1 cut(s) 918
BtsCI GGATG 2 cut(s) 547, 681
BtsI GCAGTG 2 cut(s) 36, 81
BtsIMutI CAGTG 5 cut(s) 36, 48, 81, 108, 682
Cac8I GCNNGC 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 641
Csp6I GTAC 1 cut(s) 126
CviAII CATG 1 cut(s) 174
CviQI GTAC 1 cut(s) 126
DdeI CTNAG 1 cut(s) 28
DpnI GATC 6 cut(s) 273, 401, 468, 751, 894, 917
DpnII GATC 6 cut(s) 271, 399, 466, 749, 892, 915
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 641
Eco88I CYCGRG 1 cut(s) 858
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 1 cut(s) 177
FatI CATG 1 cut(s) 173
FbaI TGATCA 1 cut(s) 466
Fnu4HI GCNGC 3 cut(s) 408, 459, 462
FokI GGATG 2 cut(s) 534, 668
Fsp4HI GCNGC 3 cut(s) 408, 459, 462
FspBI CTAG 3 cut(s) 302, 756, 922
GluI GCNGC 3 cut(s) 408, 459, 462
GsuI CTGGAG 2 cut(s) 762, 895
HapII CCGG 1 cut(s) 886
Hin1II CATG 1 cut(s) 177
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HinfI GANTC 3 cut(s) 17, 634, 698
HpaI GTTAAC 1 cut(s) 480
HpaII CCGG 1 cut(s) 886
HphI GGTGA 2 cut(s) 575, 643
Hpy166II GTNNAC 2 cut(s) 359, 480
Hpy188I TCNGA 4 cut(s) 153, 233, 527, 765
Hpy188III TCNNGA 2 cut(s) 322, 860
Hpy8I GTNNAC 2 cut(s) 359, 480
HpyCH4III ACNGT 3 cut(s) 52, 352, 370
HpyCH4IV ACGT 2 cut(s) 292, 656
HpyCH4V TGCA 7 cut(s) 259, 380, 410, 461, 497, 519, 620
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 28
HpySE526I ACGT 2 cut(s) 292, 656
Hsp92II CATG 1 cut(s) 177
Ksp22I TGATCA 1 cut(s) 466
KspAI GTTAAC 1 cut(s) 480
Kzo9I GATC 6 cut(s) 271, 399, 466, 749, 892, 915
LguI GCTCTTC 1 cut(s) 93
LpnPI CCDG 9 cut(s) 105, 173, 307, 317, 375, 614, 792, 859, 899
Lsp1109I GCAGC 3 cut(s) 394, 445, 473
LweI GCATC 1 cut(s) 690
MaeI CTAG 3 cut(s) 302, 756, 922
MaeII ACGT 2 cut(s) 292, 656
MaeIII GTNAC 4 cut(s) 46, 79, 106, 364
MalI GATC 6 cut(s) 273, 401, 468, 751, 894, 917
MboI GATC 6 cut(s) 271, 399, 466, 749, 892, 915
MboII GAAGA 5 cut(s) 64, 80, 215, 845, 909
MluCI AATT 3 cut(s) 22, 130, 417
MmeI TCCRAC 2 cut(s) 404, 505
MseI TTAA 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 1 cut(s) 886
MspR9I CCNGG 1 cut(s) 887
MwoI GCNNNNNNNGC 1 cut(s) 38
NciI CCSGG 1 cut(s) 887
NdeII GATC 6 cut(s) 271, 399, 466, 749, 892, 915
NlaIII CATG 1 cut(s) 177
NmuCI GTSAC 3 cut(s) 46, 79, 106
PaeR7I CTCGAG 1 cut(s) 858
PciSI GCTCTTC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 17, 634, 698
PflFI GACNNNGTC 1 cut(s) 104
PkrI GCNGC 3 cut(s) 409, 460, 463
Ppu21I YACGTR 1 cut(s) 293
PspPI GGNCC 1 cut(s) 641
PstI CTGCAG 2 cut(s) 412, 463
PsyI GACNNNGTC 1 cut(s) 104
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 1 cut(s) 479
SatI GCNGC 3 cut(s) 408, 459, 462
Sau3AI GATC 6 cut(s) 271, 399, 466, 749, 892, 915
Sau96I GGNCC 1 cut(s) 641
ScrFI CCNGG 1 cut(s) 887
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 3 cut(s) 408, 459, 841
Sfr274I CTCGAG 1 cut(s) 858
SinI GGWCC 1 cut(s) 641
SlaI CTCGAG 1 cut(s) 858
SmlI CTYRAG 3 cut(s) 92, 692, 858
SmoI CTYRAG 3 cut(s) 92, 692, 858
Sse9I AATT 3 cut(s) 22, 130, 417
SsiI CCGC 1 cut(s) 770
SspMI CTAG 3 cut(s) 302, 756, 922
StyD4I CCNGG 1 cut(s) 885
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 3 cut(s) 52, 352, 370
TaiI ACGT 2 cut(s) 295, 659
TaqI TCGA 4 cut(s) 99, 752, 859, 895
TasI AATT 3 cut(s) 22, 130, 417
TfiI GAWTC 3 cut(s) 17, 634, 698
Tru1I TTAA 1 cut(s) 479
Tru9I TTAA 1 cut(s) 479
TscAI CASTG 5 cut(s) 43, 55, 88, 115, 682
TseFI GTSAC 3 cut(s) 46, 79, 106
TseI GCWGC 3 cut(s) 407, 458, 461
Tsp45I GTSAC 3 cut(s) 46, 79, 106
TspDTI ATGAA 4 cut(s) 64, 162, 300, 661
TspGWI ACGGA 1 cut(s) 719
TspRI CASTG 5 cut(s) 43, 55, 88, 115, 682
Tth111I GACNNNGTC 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 641
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 607
XhoI CTCGAG 1 cut(s) 858
XspI CTAG 3 cut(s) 302, 756, 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.