pycom06g21550
NAC Family

Nascent polypeptide-associated complex subunit beta

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011463
Physical Location & Seq
Reverse (-)
24125588 .. 24127695
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGATTGGGGATGGATTATTGAGCAGAGCACCCACTATGAATAGGGAGAAACTTATGAAGATGGCTGGTGCGGTTCGCACTGGTGGAAAGGGCAGCGTGAGGAGAAAGAAGAAGGCTGTTCACAAGACAACTACCACAGACGACAAACGGCTTCAGAGCACCCTCAAGAGGATAGGGGTGAATGCTATTCCTGCAATCGAGGAAGTTAACATATTCAAGGATGATGTAGTTATCCAGTTCCTAAACCCCAAAGTTCAAGCTTCAATTGCTGCCAACACCTGGGTTGTTAGCGGTTCTCCTCAAACAAAGAAGTTGCAAGATATTCTTCCCAGTATTATCAGCCACTTGGGGCCAGATAACTTGGACAATTTGAAGAAGTTGGCTGAGCAGTTCCAGAAGCAAGCACCTGGTGCTGGTGGAGCCACTGCACAAGAGGACGATGATGATGAGGTCCCTGAGCTTGTGGCCGGGGAGACCTTTGAAGCTGCTGCGGAGGAGAAGGACAAGGAGGAGAATCAAGCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04214 map04214
Pfam Domains
Protein Families
NAC

Protein Analysis

176

Amino Acids

18.92

Weight (kDa)

6.17

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAC PF01849 48 - 103 8.9e-20 NAC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 279
AciI CCGC 3 cut(s) 71, 291, 491
AcoI YGGCCR 1 cut(s) 465
AcuI CTGAAG 1 cut(s) 137
AdeI CACNNNGTG 1 cut(s) 410
AfiI CCNNNNNNNGG 3 cut(s) 168, 279, 413
AgsI TTSAA 5 cut(s) 217, 257, 264, 373, 482
AjnI CCWGG 2 cut(s) 278, 406
AluBI AGCT 4 cut(s) 260, 460, 485, 521
AluI AGCT 4 cut(s) 260, 460, 485, 521
Alw21I GWGCWC 2 cut(s) 31, 161
Alw26I GTCTC 1 cut(s) 467
AoxI GGCC 2 cut(s) 350, 465
ApeKI GCWGC 4 cut(s) 93, 269, 485, 488
AspS9I GGNCC 2 cut(s) 350, 451
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 1 cut(s) 190
AvaII GGWCC 1 cut(s) 451
Bbv12I GWGCWC 2 cut(s) 31, 161
BbvI GCAGC 4 cut(s) 105, 256, 472, 475
BccI CCATC 2 cut(s) 5, 55
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 2 cut(s) 280, 408
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 467
BisI GCNGC 4 cut(s) 94, 270, 486, 489
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 4 cut(s) 95, 271, 487, 490
Bme1390I CCNGG 3 cut(s) 280, 408, 469
Bme18I GGWCC 1 cut(s) 451
BmgT120I GGNCC 2 cut(s) 350, 451
BmiI GGNNCC 3 cut(s) 351, 421, 453
BmrFI CCNGG 3 cut(s) 280, 408, 469
BmrI ACTGGG 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 456
Bpu1102I GCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 469
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 2 cut(s) 279, 468
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 279, 413
Bse1I ACTGG 3 cut(s) 85, 235, 330
BseBI CCWGG 2 cut(s) 280, 408
BseDI CCNNGG 2 cut(s) 279, 468
BseGI GGATG 2 cut(s) 16, 226
BseLI CCNNNNNNNGG 3 cut(s) 168, 279, 413
BseMII CTCAG 2 cut(s) 375, 447
BseNI ACTGG 3 cut(s) 85, 235, 330
BseRI GAGGAG 4 cut(s) 115, 288, 509, 524
BseXI GCAGC 4 cut(s) 105, 256, 472, 475
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 2 cut(s) 352, 467
BsiHKAI GWGCWC 2 cut(s) 31, 161
BsiSI CCGG 1 cut(s) 468
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 3 cut(s) 168, 279, 413
BsmAI GTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 437
BsmI GAATGC 1 cut(s) 187
BsnI GGCC 2 cut(s) 352, 467
Bso31I GGTCTC 1 cut(s) 467
Bsp1286I GDGCHC 2 cut(s) 31, 161
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 3 cut(s) 71, 291, 491
BspANI GGCC 2 cut(s) 352, 467
BspCNI CTCAG 2 cut(s) 376, 448
BspLI GGNNCC 3 cut(s) 351, 421, 453
BspTNI GGTCTC 1 cut(s) 467
BsrI ACTGG 3 cut(s) 85, 235, 330
BssECI CCNNGG 2 cut(s) 279, 468
Bst2UI CCWGG 2 cut(s) 280, 408
BstAPI GCANNNNNTGC 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 3 cut(s) 384, 456, 525
BstF5I GGATG 2 cut(s) 16, 226
BstMAI GTCTC 1 cut(s) 467
BstMWI GCNNNNNNNGC 4 cut(s) 191, 266, 410, 419
BstNI CCWGG 2 cut(s) 280, 408
BstSCI CCNGG 3 cut(s) 278, 406, 467
BstV1I GCAGC 4 cut(s) 105, 256, 472, 475
BsuRI GGCC 2 cut(s) 352, 467
BtsCI GGATG 2 cut(s) 16, 226
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 2 cut(s) 78, 423
Cac8I GCNNGC 1 cut(s) 402
Cfr13I GGNCC 2 cut(s) 350, 451
CsiI ACCWGGT 1 cut(s) 406
DdeI CTNAG 3 cut(s) 384, 456, 525
DraIII CACNNNGTG 1 cut(s) 410
EaeI YGGCCR 1 cut(s) 465
Eco31I GGTCTC 1 cut(s) 467
Eco47I GGWCC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 451
EcoRII CCWGG 2 cut(s) 278, 406
FaiI YATR 3 cut(s) 38, 56, 212
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 437
Fnu4HI GCNGC 4 cut(s) 94, 270, 486, 489
FokI GGATG 2 cut(s) 23, 233
Fsp4HI GCNGC 4 cut(s) 94, 270, 486, 489
GluI GCNGC 4 cut(s) 94, 270, 486, 489
HaeIII GGCC 2 cut(s) 352, 467
HapII CCGG 1 cut(s) 468
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 2 cut(s) 258, 519
HinfI GANTC 1 cut(s) 514
HpaI GTTAAC 1 cut(s) 208
HpaII CCGG 1 cut(s) 468
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 2 cut(s) 121, 208
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 166, 394
Hpy8I GTNNAC 2 cut(s) 121, 208
HpyAV CCTTC 2 cut(s) 106, 493
HpyCH4V TGCA 3 cut(s) 194, 316, 428
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 266, 410, 419
HpyF3I CTNAG 3 cut(s) 384, 456, 525
KspAI GTTAAC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 419
Lsp1109I GCAGC 4 cut(s) 105, 256, 472, 475
MabI ACCWGGT 1 cut(s) 406
MboII GAAGA 4 cut(s) 70, 121, 317, 385
MfeI CAATTG 1 cut(s) 264
MhlI GDGCHC 2 cut(s) 31, 161
MluCI AATT 2 cut(s) 264, 367
MnlI CCTC 9 cut(s) 93, 162, 173, 193, 309, 427, 442, 487, 502
MseI TTAA 1 cut(s) 207
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 3 cut(s) 280, 408, 469
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 187
MvaI CCWGG 2 cut(s) 280, 408
MwoI GCNNNNNNNGC 4 cut(s) 191, 266, 410, 419
NciI CCSGG 1 cut(s) 469
NlaIV GGNNCC 3 cut(s) 351, 421, 453
PctI GAATGC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 514
PflMI CCANNNNNTGG 1 cut(s) 279
PkrI GCNGC 4 cut(s) 95, 271, 487, 490
PpuMI RGGWCCY 1 cut(s) 451
Psp5II RGGWCCY 1 cut(s) 451
Psp6I CCWGG 2 cut(s) 278, 406
PspGI CCWGG 2 cut(s) 278, 406
PspN4I GGNNCC 3 cut(s) 351, 421, 453
PspPI GGNCC 2 cut(s) 350, 451
PspPPI RGGWCCY 1 cut(s) 451
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 4 cut(s) 94, 270, 486, 489
Sau96I GGNCC 2 cut(s) 350, 451
ScrFI CCNGG 3 cut(s) 280, 408, 469
SduI GDGCHC 2 cut(s) 31, 161
SetI ASST 8 cut(s) 262, 281, 409, 453, 462, 479, 487, 523
SexAI ACCWGGT 1 cut(s) 406
SinI GGWCC 1 cut(s) 451
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 2 cut(s) 264, 367
SsiI CCGC 3 cut(s) 71, 291, 491
StyD4I CCNGG 3 cut(s) 278, 406, 467
TaqI TCGA 1 cut(s) 198
TasI AATT 2 cut(s) 264, 367
TfiI GAWTC 1 cut(s) 514
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 2 cut(s) 85, 430
TseI GCWGC 4 cut(s) 93, 269, 485, 488
TspDTI ATGAA 2 cut(s) 53, 71
TspRI CASTG 2 cut(s) 85, 430
Van91I CCANNNNNTGG 1 cut(s) 279
VpaK11BI GGWCC 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.